BIOL 3340.01, Lab
Summer 08, Quiz 2A
Name:_____________________
1. Which of the following is the overall objective for Unit 2?
a. learn how to identify bacteria by biochemical testing
b. basic culturing techniques using agar plates and broths
c. examine the factors controlling the growth of bacteria
d. use of the light microscope for viewing stained microorganisms
2. Which of the following microscopes will be used in this lab for the routine
observation of bacteria, yeast and other microbes?
a. simple lens microscope
b. compound light microscope
c. phase contrast microscope
d. compound dark field microscope
3. Resolution of a microscope can be improved by decreasing the wavelength of
light used and by increasing the amount of light that is gathered by the objective
lens. What is the property of gathering light known as?
a. spherical aberration
b. total magnification
c. numerical aperture
d. chromatic aberration
4. The resolution limit (resolving power) of a bright field microscope is 0.22 μm. If
the space between two objects in close proximity to each other is less than .22
um, what will you observe?
a. the two objects will appear as a single object
b. the two objects will appear as two separate objects
c. the two objects will appear as three separate objects
d. none of the above
5. Whereas the ability to see detail is called resolution, the ability to enlarge an
image of a specimen is called:
a. amplification
b. magnification
c. inflation
d. exacerbation
6. Which of the following is NOT a lens in the compound microscope?
a. ocular lens (eyepiece) that magnifies the image ten times actual size
b. condenser lens that focuses light onto to the specimen
c. objective lens that magnifies the image from 4 to 100 times actual size
d. illuminating lens that emits light onto the specimen
7. The oil immersion lens on a microscope has the highest magnification. If it is
being used to view a specimen, what is the total magnification of the image
assuming the lenses in the eyepiece magnify 10x?
a. 10 times actual size
b. 100 times actual size
c. 1000 times actual size
d. 2000 times actual size
8. Which of the following is an uncommon bacterial shape?
a. bacillus
b. coccus
c. columnar
d. spirillum/spirochete
9. Crystal violet, iodine, acetone-alcohol and safranin are the staining reagents
used for which of the following common bacterial stains?
a. capsule stain
b. Gram stain
c. endospore stain
d. acid fast stain
10. Which stain would you use to stain and see the internal structures produced
by members of Bacillus and Clostridium after they sporulate?
a. flagella stain
b. capsule stain
c. endospore stain
d. Acid fast stain