The Effects of Exercise of Male and Female Pulse Count and Blood Pressure By: David Pfeilsticker April 7, 2013 TA: JINLING LIU Introduction: The human circulatory system is a collection of tiny structures through which blood flows. The blood is used as a medium to supply tissues with oxygen and nutrients for physical activity, metabolism, growth, and waste removal. When a person exercises, his or her body focuses on the immediate demand for gas exchange and adapts accordingly. The body adapts by increasing heart rate and blood pressure to accommodate the physical demand (“Cardiovascular Physiology”). Gas exchange is a complex process that involves two body systems: the circulatory system and the respiratory system. The circulatory system consists of the heart, blood vessels, and blood. The second system involved in gas exchange is the respiratory system, which consists of the lungs, trachea, and bronchia tree. The organs of the circulatory system and respiratory system that play a role in gas exchange work together to insure that all tissues in the body receive an adequate amount of oxygen and that excess carbon dioxide is removed (“Cardiovascular Physiology”) The circulatory system is a lot more complex than most people realize. The heart is the core of the circulatory system. The heart consists of four chambers: two atria and two ventricles. The ventricles pump the blood and the atria collect the blood. Blood is pumped out of the right ventricle and though the pulmonary semilunar valve. After this, the blood flows though the pulmonary arteries to the lungs. In the lungs, gas exchange occurs in the capillary beds. This process is known as the pulmonary circuit. Blood then returns to the heart though pulmonary veins and enters the left atrium, where it passes though the left atrioventricular valve, and then into the left ventricle. The left ventricle then pumps blood though the aortic semilunar valve and into the aorta. The aorta distributes the blood to the upper and lower body, and gas exchange occurs in the capillary beds of the body’s tissues. This process is known as the systemic circuit. Lastly, the blood returns to the heart thought the superior and inferior vena cava, and enters the right atrium (Campbell and Reece 898-927). Like many things in the body, heartbeat is coordinated. First atria contract together, following that, the ventricles contract together. The heart sounds we hear are the results of the contractions, but are made from the closing of valves. The closing of valves between atria and ventricles causes the first low “lub” sound, as the ventricles contract. The closing of the pulmonary and aortic semilunar valves causes the second “dub” sound as the ventricles relax (“Cardiovascular Physiology”). Blood pressure is also an interesting phenomenon associated with the circulatory system. Cardiac output and peripheral resistance are the two factors responsible for blood pressure in the arteries. Cardiac output is the amount of blood pumped by the left ventricle per unit time. Peripheral resistance is the resistance to flow though arterioles and capillaries that keeps the pressure from dropping to zero between heartbeats. The contraction of the left ventricle causes a high peak in blood pressure. This high peak in blood pressure is called the systolic pressure. The lowest level of blood pressure is caused by ventricular relaxation and filling. This is termed diastole. It is worth noting that blood pressure is high in arteries nearest to the heart (“Cardiovascular Physiology”). It is common knowledge that when a person sprints up a flight of stairs, his or her breathing rate increases. But does this change physiology differ in men and woman? The purpose of this experiment was to find the effect of physical activity on heart rate and blood pressure, and to see how this phenomenon differed between males and females. The hypothesis that our lab group formulated was that there would be a change in both pulse and systolic/diastolic blood pressure. The difference would arise in the rate at which they increase. Men having less of a percent change than women due to the greater physical condition and expected work load. Bigger muscle mass will contribute to less “work” put into exercising and therefore a lower response by the heart. Materials and Methods: The materials that were used in this experiment included: a stethoscope, a sphygmomanometer, a clock, alcohol swabs, a wooden stepping box, and a metronome. The initial data regarding age, sex, weight, height, alcoholic drinks a week, tobacco products smoked a week, caffeinated drinks consumed a week, and exercise per week was collected a week before the experiment was conducted. However, this experiment mainly focused on the difference between pulse count, and systolic/diastolic blood pressure after exercise in regards to gender. Students had a chance to practice using a sphygmomanometer to measure blood pressure before the actual experiment took place to familiarize with the equipment. The class was divided into six groups that consisted of four group members. Each group member was assigned to a task; to either, keep track of the time, count the pulse, or measure the blood pressure. All group members had to partake in the experiment and their resulting data was taking into account. All group members worked simultaneously; while one was measuring the pulse count, the other would measure his blood pressure. On the day of the experiment, each group member measured his or hers resting heart rate and resting blood pressure. Additionally, both systolic and diastolic blood pressures were taken by another student. At the conclusion of the experiment, blood pressure and heart rate were calculated for each group member after completing the step exercise for 15 steps a minute, and then 30 steps a minute. A metronome was used during the step exercise to calibrate the steps. Then we compiled our class data with two other class sections and analyzed the results. All the data was compiled together and put into Microsoft Excel. A crucial thing to remember is that the data obtained for the pulse count of each individual participant was for 30 seconds interval, and not per minute. The experimental group consisted of 30 females and 23 males of age ranging from 18 to 26. Calculations were made to determine standard deviation, averages, percent differences, and standard error. We then put the data into graphs and tables to be further analyzed. Results: After the process of experimentation, the data was analyzed and the results are as follows... Graphs showing the resting values of the experiment, as well as others that display the average percent difference in varies categories. ^As you can see, the values for pulse count are around the same area for both males and females. Systolic and Diastolic BP for males seems to be slightly larger. ^The percent difference between males and females pulse count was not that far off either. Both hovering around the same area. ^This is where the main difference between males and females is. The percent difference in the systolic BP after exercise seems to differ greatly. The standard error bars do not over lap which means there is some variation within the data. ^Although there may seem like this is some variation within the percent difference between the diastolic BP, the standard error bars do overlap, so it is possible that there is no variation in the data. Also the percent difference is very small to begin with. The data that was displayed in graphic form can also be looked at in table form... Tables for Data Collection - Exercise Physiology Lab - Bio 240W Spring 2008 Resting Level Values: Table 1: Resting level values: Average Pulse count Sys.BP Dias.BP Males 35.627 131.08 81.26 Females 36.31 121.3 76.56 SE Males 0.937 2.77 2.688 Females 0.902 2.387 2.06 Exercise Data: Table 2: Normalized average change in pulse count/30sec after exercise av. % diff Pulse count 15-steps 30-steps Males 19.205 54.473 Exercise Data: Table 3: Normalized average change in systolic After looking at the BP after exercise Females 18.338 51.45 SE Males 4.79 5.779 Females 3.79 5.162 average numbers and average percent differences, we can av. % diff SE then look at the results from the TTEST thatFemales was performed to achieve our Females P values. P Sys.BP Males Males 15-steps 9.772 2.07 2.299 1.235 values .05 show that the two data sets2.68 (male and female), 30-stepsaround or below 19.3 12.64 1.96 for a certain category, show some statistical significance. Meaning that there is a significant change Exercise Data: in the 4: data and it should be examined. The P values from the TTEST are as followed. Table Normalized average change in diastolic BP after exercise av. % diff Dias.BP 15-steps 30-steps Males 4.32 5.61 Females -2.18 -0.0578 SE Males 2.95 3.44 Females 2.689 2.776 P Values Resting % Diff. 15 Steps % Diff. 30 Steps Pulse Count 0.606 0.886 0.699 Significant (Y/N) N N N Systolic BP ^As you can see, the only significant P values would be the ones pertaining to the systolic BP. This 0.009 0.0028 0.046 means betweenSignificant the two groups there is a meaningful difference that occurs in the (Y/N) (male Y and female) Y Y BP before and during exercise. Diastolic BP systolic 0.1044 0.108 0.2011 Significant (Y/N) N N N Also, there are other factors that could pertain to that data the was presented above. All participants were required to fill out a general lifestyle form which asked for sex, height, weight, tobacco products smoked a week, caffeine drinks and day, alcoholic drinks consumed a week, and day exercised a week. Since we analyzed the data from a male and female perspective the data will be displayed in the a similar manner. An average of the numbers will be used. Age Height Smoke/W Caffeine/ Alcohol/ Exercise k Day Wk for the (Day/Wk) ^Differences arise in weight, height, smoke/wk, alcohol/wk, and exercise. Although smoking Males 20.73 Females 20 Discussion: Weight category for males there is an outlier with the value of 50. 173.91 70.91 2.956 1.44 8.22 2.86 134.1 64.4 0.166 1.2 4.733 3.56 The data that was collected shows promising results. The biggest coming with the findings of multiple significant p values for systolic blood pressure. Although there is some variation within the other data collected, this is what gives us our results. The average resting values for pulse count and diastolic blood pressure seem to be the same for both males and females, but there is some variation with the resting systolic blood pressure since the p value is .009. This is good since it somewhat makes it easier to compare data at the end of the experiment and also made the findings more uniform, with less chance of error. Also, the average percent difference in pulse count after exercise for both males and females is also consistent within one another. This means that all of the variation should arise in the different blood pressure calculations that have a constant and consistent equal pulse rate with one another. Looking at the average percent difference in systolic blood pressure, you can find variation between the two data sets (male and female.) The standard error bars do not overlap within the graphs which shows a reliable indication of statistical difference. Also, the fact the p values for systolic blood pressure are all below .05 gives us the ultimate indication that there is some statistical significance between the two groups. Analyzing the average percent difference in diastolic blood pressure we can see that it seems as if there is some variation, but the percent difference is very small. The p values are also close to .05 but do not go any lower than .1 which would not qualify as statistically significant in the terms that we are using. By only looking at the average data and not the individual data, we can see that there is some statistical significance between males and females when it comes to the average percent difference in systolic blood pressure. It seems as if during exercise, the pulse rate of both men and women increase at the same rate and the diastolic blood pressure also changes in consistency with one another. The systolic blood pressure which was different between the two groups in the beginning of the experiment still stays different, but does not change consistently within males and females. Meaning, the systolic blood pressure starts off different and rises on different levels for males and females during exercise. Comparing these findings with the average individual data such as height and weight we can try to figure out what causes such differences in systolic blood pressure. The average age and caffeine drinks are around the same value. Average tobacco products a week for males is exaggerated a bit since there is one outlier with a value of 50 that drives up all the other values, because of this the smoke/wk category can be treated the same and constant. The males have a larger average height, weight, and alcohol/wk consumption, while women seem to exercise more. These categories such as exercise and moderate alcohol consumption, can lower your blood pressure while being overweight can help increase your blood pressure. Although we have specific data for each category it is outlandish to just say that because women exercise .7 more days a week their systolic blood pressure should be statistically different than men. The real cause for the difference in systolic blood pressure is found within the hight and weight difference of the two groups. Men being taller and weighing more have been found to naturally have a higher blood pressure. A study conducted to find the difference between the blood pressure in genders found that women have a lower blood pressure in peripheral arteries than males due to the shorter body height, which in turn means shorter distance to reflecting sites and an increase in aortic tightening (London, 1995). Besides a difference in the height and weight of the two genders, there are other factors that need to be looked at. Men and women both produce a different amount of gender specific chemicals such as testosterone and estrogen, and it may certainly be that the combination of certain differences in the life styles between men and women and the natural physical differences between the genders that ultimately contributes to the difference in blood pressure. Either way, we have come to the conclusion that the systolic blood pressure in males is statistically different that that of females while resting and during exercise. Which is somewhat different than our lap groups original hypothesis. Although we would like to think our results are 100% correct, in reality they may be a little skewed. Students that took blood pressure measurements are not professionals at using the correct apparatus for doing so, and because of this, our blood pressure numbers might be a little off, which would have a pretty sounding effect on our data. Also, pulse counts could have been miscounted which would also skewed our results. Even though there might have been some error within our experiment, I would like to think that we did an acceptable job in recording accurate data and that our research would not go unnoticed. It would be very interesting if experiments were done to see the effects of testosterone and estrogen, and other generally gender specific chemicals effect blood pressure, and to correlate that with the specific life style of participants, in such a way as we did in this experiment. Blood pressure and vascular health is one of the most important aspects of physical health that people should be concerned about. If blood can’t get to a certain location then neither can the nutrients that use it as a medium of transportation. Everybody has a heart, why shouldn’t we try to find out all that we can about it and better our own understanding of ourselves. References: Campbell, Neil A., Jane B. Reece, Lisa A. Urry, Michael L. Cain, and Steven A. Wasserman. Biology. Eighth ed. San Francisco: Pearson, 2008. 898-927. Print. “Cardiovascular Physiology: The relationship between Gas Exchange and Cardiac Activity” Edited by Nelson, K, and Burpee, D. Department of Biology, The Pennsylvania State University, PA (2013). London, Gerard, Alain Guerin, Bruno Bruno Pannier, Sylvain Marchais, and Michael Stimpel. "Influence of Sex on Arterial Hemodynamics and Blood Pressure." PubMed (1995). Print.