CELL AND MOLECULAR BIOLOGY BIOL305.001 (FALL 2015)
PROFESSOR: Dr. William J. Mackay
OFFICE: Cooper Hall Room 168
TELEPHONE: 732-2926
OFFICE HOURS: MW 5:15pm – 6:30pm; T Th 4:45pm – 6:00pm
Email: wmackay@edinboro.edu
TEXT: Cell and Molecular Biology, Gerald Karp (seventh edition) (John Wiley & Sons, Inc.)
(2013), ISBN-13: 978-1118-20673-1
A Laboratory Course in Cell & Molecular Biology, Modern Biology Series 5, John N. Anderson
(1993; 2007) http://www.wjmmackay.com/cellmol-labs.html
DR. MACKAY’S WEB PAGE: www.wjmmackay.com
Class Notes: http://www.wjmmackay.com/cellmol-notes.html
TENTATIVE LECTURE/LAB SCHEDULE
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
M
W
M
W
24 AUG
26 AUG
31 AUG
02 SEP
RELEVANT READINGS
Introduction/The Chemical Basis of Life
The Chemical Basis of Life/Bioenergetics
Bioenergetics, Enzymes, and Metabolism/DNA
LAB: Anatomy & Evolution of the Genome EXP-1001
Background Lab Information
M 07 SEP LABOR DAY (NO CLASS)
W 09 SEP LAB: Analysis of a Mutant Hemoglobin Gene IND-6B
M 14 SEP Nature of the Gene and the Genome/DNA Replication
W 16 SEP LAB: Genotype to Phenotype – Part 1 EXP-1004
M 21 SEP Gene Expression: From Transcription to Translation
W 23 SEP LAB: Genotype to Phenotype – Part 2 EXP-1004
Purification of Plasmid DNA
M 28 SEP The Structure & Function of the Plasma Membrane
W 30 SEP LAB: Electrophoretic Separation of Proteins EXP-101
M 05 OCT Cell Signaling & Signal Transduction
W 07 OCT EXAM 1
M 12 OCT Cytoplasmic Membrane Systems
W 14 OCT LAB: Serum Proteins Using Western Blots – 1 EXP-801
M 19 OCT Interactions Between Cells & Their Environment
Aerobic Respiration and the Mitochondrion
Photosynthesis & the Chloroplast
W 21 OCT LAB: Serum Proteins Using Western Blots – 2 EXP-801
M 26 OCT Photosynthesis & the Chloroplast
The Cytoskeleton & Cell Motility
W 28 OCT EXAM 2
M 02 NOV Prokaryotic Genome/ DNA Repair
W 04 NOV LAB: Enzyme Cytochemistry EXP-701
M 09 NOV Recombinant DNA Technology
Chapters 1, 2
Chapters 2, 3
Chapters 3, 10
pp. 129-140
pp. 6-16; 110-128
Chapters 10, 13
pp 162-174
Chapter 11
pp 162-174
handout
Chapter 4
Chapter 15
Chapter 8
pp. 24-34
Chapter 7
Chapter 5
Chapter 6
pp. 24-34
Chapter 6
Chapter 9
Chapters 12, 13
pp 61 - 75
Chapter 18
24
25
26
27
28
29
29
W
M
W
M
M
W
M
11 NOV
16 NOV
18 NOV
23 NOV
30 NOV
02 DEC
07 DEC
LAB: Localizing Tubulin by Immunohistochemistry 1 IND-26
Cellular Reproduction
Chapter 14
LAB: Localizing Tubulin by Immunohistochemistry 2 IND-26
Cancer
Chapter 16
Cancer
Chapter 16
MAKE-UP EXAM
FINAL EXAM (6-8PM CH141)
1. Determination of grade:
Quizzes* (see below)
Exam 1
Exam 2
25
25
25
FINAL Exam
Attendance** (see below)
25
0-5
TOTAL
105
A: 90 – 105
B+: 85 – 89
B: 80 – 84
C+: 75 – 79
C: 70 - 74
D+: 65 - 69
D: 60 - 64
F: < 60
2. *Weekly lecture quizzes: There will be periodic ten-minute quizzes, which will cover
lecture/lab material from the previous quiz. Final quiz points will be determined as follows:
Total quiz points  [# of quizzes X 10] X 25 = final quiz points. [e.g. (108 total quiz points 
12 quizzes) = 22.5 final quiz points
3. Examinations: Examinations 1, 2, and the final exam WILL be given on the dates specified.
You will have three hours to take each exam. Any question format may be expected on the
exams. Examinations will be primarily based on information presented in the lecture/lab
sessions. Each exam, however, will also contain information (5 - 25%) that will be drawn from
textbook readings and handouts that may not have been directly covered in lecture or lab. Thus,
it is to your benefit to read the assigned chapters in your textbook.
4. **Absences: Students may attend lectures and labs at their own discretion. In accordance
with University policy, attendance will be taken at each lecture/lab meeting. Students who
maintain a perfect lecture/lab attendance will receive five bonus points to your final grade.
Students that have 1 absence (for any reason) will earn 3 points and students that have 2 absences
(for any reason) will earn 1 point to your final grade. It is my experience that students with 3 or
more absences rarely obtain a grade higher than C. In short, it is to your benefit to attend all
lecture and lab sessions.
5. Make-up work: Students are required to take all exams and to complete all assignments on
time. Exceptions will be made in rare and unusual circumstances and only by PRIOR
arrangement. Each case will be considered individually. Make-up exams, when justified, will be
given at the earliest mutually convenient time.