Cells and Cellular Organization II. Cell Theory A. All living things are made of a single cell (bacteria) or many cells (most other organisms). 1. A single - celled organism is unicellular 2. A many – celled organism is multicellular B. A cell is the smallest unit of life, nothing smaller than a cell is considered living. C. All cells come from pre – existing cells through cell division III. Cell Structure A. Surface Area: Volume Ratio 1. Cells obtain substances (such as oxygen and glucose) by absorption. 2. Cells get rid of substances (carbon dioxide and wastes) by secretion. 3. Since cells have no complex method of transport, the cell must be small enough for the surface area of the cell to service the contents of the cell. 4. Cell size is limited. Cells must remain small. 5. Cells need large surface area: volume ratios B. Cell Shape 1. Cells keep shape with a skeleton just like yours (cytoskeleton) C. Cell Membrane 1. All cells have cell membranes that surround the cell to regulate contents going in and coming out. The membrane allows absorption of glucose and oxygen and allows excretion of carbon dioxide and wastes. The cell membrane is made of phospholipids, which makes sense because the membrane must be resistant to breakdown by water, which surrounds the cell. Selectively permeable. 2. Some cells (plant, bacteria) also have cell walls for support 3. Cytoplasm in the cell is a fluid that allows absorption and diffusion C. Cell Size 1. Cell size varies depending on the organism and function of the cell. 2. Most are microscopic, but some can be 1.0 meter in length. IV. Cell Types Prokaryotic No Nucleus Ribosomes, mitochondria Bacteria Eukaryotic Nucleus present Many organelles present Animal, Plant, Fungus Protist A. Prokaryotic 1. Lack organization, no nucleus, one single strand of DNA 2. Bacteria are examples B. Eukaryotic 1. Highly organized, many organelles, nucleus present 2. All multicellular organisms are made of eukaryotic cells, as well as some unicellular organisms C. Comparison of Prokaryotic and Eukaryotic Cells Please Play ME! Comparison of Prokaryote, Animal and Plant Cells by Rodney F. Boyer Cell Variation Color images of histological sections home page: http://www.udel.edu/Biology/Wags/histopage/colorpage/colorpage .htm Adipose http://www.udel.edu/Biology/Wags/histopage/colorpage/ca/wav.G IF Neuron http://www.udel.edu/Biology/Wags/histopage/colorpage/cne/cnem nvhl.GIF Sperm http://www.udel.edu/Biology/Wags/histopage/colorpage/cmr/cmrst 6.GIF Stratified Squamous Epithelium - Skin http://www.udel.edu/Biology/Wags/histopage/colorpage/cep/cepss q.GIF Ciliated epithelium http://www.udel.edu/Biology/Wags/histopage/colorpage/cep/cepcp e.GIF Skeletal muscle http://www.udel.edu/Biology/Wags/histopage/colorpage/cmu/cmus tmlt1.GIF Cardiac muscle http://www.udel.edu/Biology/Wags/histopage/colorpage/cmu/cmu cmid.GIF Cartilage http://www.udel.edu/Biology/Wags/histopage/colorpage/cc/ccfls.G IF Bone http://www.udel.edu/Biology/Wags/histopage/colorpage/cb/cblc.G IF Blood cells http://www.udel.edu/Biology/Wags/histopage/colorpage/ch/chbpe. GIF Elodea http://iws.ccccd.edu/biopage/BioLab/Unit%207/Elodea%20cells% 2010x.jpg Onion http://www.blc.arizona.edu/INTERACTIVE/cells3l/euk2.gif V. Cell Function A. A single cell functions as a self – sustaining unit B. Structures of the cell 1. Cell Membrane: (common to all cells) made of nonpolar phospholipids http://home.earthlink.net/~shalpine/anim/Life/memb.htm http://www.wiley.com/legacy/college/boyer/0470003790/a nimations/cell_structure/cell_structure.htm 2. Cell Wall: rigid, reinforced with protein and cellulose. http://micro.magnet.fsu.edu/cells/plants/cellwall.html 3. Cytoplasm: fluid, inner portion of a cell, acts as support and a transport media 4. Nucleus: only found in eukaryotes, contains genetic information, using this genetic information it indirectly controls the cell. 5. Organelles: Take the Online Cell Tour http://www.landmark.edu/institute/grants_research/biolog y_success/celltour/index.cfm#animal.cfm Cell wall Centrioles Chloroplasts Chromosomes Cytoskeleton Endoplasmic reticulum Nuclear membrane Nucleolus Golgi apparatus Lysosome Mitochondria Plasma membrane Ribosomes Vacuoles Use the following site to answer the following questions. http://www.tvdsb.on.ca/westmin/science/sbi3a1/Cells/cells.htm 1. Look at the animal cell on the left and the plant cell on the right. List three main differences between the two. a. b. c. 2. Go to the “chloroplasts”. Play the animation. What are the reactants (compounds that the reaction starts with) and what are the products (what things are made) of photosynthesis? 3. What is the function of the chloroplast? 4. Take the quiz! Using the following website, answer the following questions about diffusion and osmosis. http://www.stolaf.edu/people/giannini/biological%20anamations.html Under “Membrane Transport” choose diffusion. 1. What is this animation trying to show about the way most molecules/atoms move? Under “Membrane Transport” choose osmosis. 2. What happens when salt is added to one side of the semipermeable membrane? Using the following website, be able to identify the following structures of the cell membrane. http://home.earthlink.net/~shalpine/anim/Life/memb.htm Play the animation. 1. What is the main molecule of the cell membrane? 2. What does hydrophilic mean? 3. What does hydrophobic mean? 4. What are the function of the membrane proteins? VI. Transport in Cells http://www.wiley.com/legacy/college/boyer/0470003790/animations/me mbrane_transport/membrane_transport.htm (home page of biological animations) http://www.stolaf.edu/people/giannini/biological%20anam ations.html transport animations http://highered.mcgrawhill.com/sites/0070271348/student_view0/chapter4/elearning.html Transport site http://www.wiley.com/legacy/college/boyer/0470003790/animatio ns/membrane_transport/membrane_transport.htm A. Passive Transport: Does NOT require energy (“it just happens!”) 1. Diffusion: Random movement of particles from a highly concentrated area to an area of low concentration until equilibrium is reached. http://www.stolaf.edu/people/giannini/flashanimat/transpo rt/project.swf 2. Facilitated Diffusion: Membrane proteins are used. The proteins are specific to a specific molecule. Good for molecules such as glucose (large molecules). http://www.stolaf.edu/people/giannini/flashanimat/transpo rt/caryprot.swf 3. Osmosis: the diffusion of water http://www.stolaf.edu/people/giannini/flashanimat/transpo rt/osmosis.swf Movement from an area of high conc. of water to an area of low conc. of water. B. Active Transport: Energy expenditure required – substances are moved against a concentration gradient. UP Phagocytosis: http://www.stolaf.edu/people/giannini/flashanimat/cellstructures /cell.swf Movement against a concentration gradient: http://www.stolaf.edu/people/giannini/flashanimat/transport/anti port.swf http://www.wiley.com/college/pratt/0471393878/student/animat ions/membrane_transport/index.html VI. Origin of Eukaryotic Cells A. Endosymbiont Theory proposed by Lynn Margulis 1. Mitochondria and Chloroplasts have the following features: a. contain their own DNA b. have 2 membranes c. reproduce independently d. DNA resembles the DNA of prokaryotic cells, not eukaryotic cells 2. Conclusion is that mitochondria and chloroplasts were at one time free – living. These free – living organelles formed a mutual association (a symbiotic relationship) with prokaryotic cells, forming the first eukaryotic cells.