Program of Study
:
General Medicine
Course
:
Biochemistry 2
Abbreviation
:
LCH/VAA20
Schedule
:
60 hours of lectures
60 hours of exercises
Course Distribution :
2nd year, 3rd.semester
Numer of Credits
:
16
Course Form
:
Lectures, exercises
Lectures :
Teachers :
Prof. RNDr. Jitka Ulrichová, CSc.
Doc. RNDr. Daniela Walterová, CSc.
Doc. Mgr. Jitka Vostálová, Ph.D.
MUDr. Norbert Cibiček, Ph.D.
Study :
Continuous
Date
1 16.09.2015
17.09.2015
2 23.09.2015
24.09.2015
3 30.09.2015
01.10.2015
4 07.10.2015
08.10.2015
5 14.10.2015
15.10.2015
6 21.10.2015
22.10.2015
7 28.10.2015
29.10.2015
Subject
Lipid metabolism – overview. Lipoproteins.
Fatty acid degradation.
Ketone bodies. Fatty acid biosynthesis.
Cholesterol metabolism. Bile acids.
Polyunsaturated fatty acids. Eicosanoids.
Steroids. Vitamin D.
Triacylglycerol, phospholipid and glycolipid
metabolism.
Insulin, glucagon – effect on carbohydrate and
lipid metabolism.
Overview of the pathobiochemistry of saccharides.
Overview of the pathobiochemistry of lipids.
Proteolysis. General pathways of amino acids
catabolism. Urea cycle.
Amino acids biosynthesis. Metabolism of aromatic
amino acids. Catecholamines.
State holiday.
Tetrapyrroles – heme biosynthesis. Bile pigments.
Iron metabolism.
No. of
less.
2
2
2
2
2
2
2
Teacher
Walterová
Vostálová
Vostálová
Vostálová
Ulrichová
Vostálová
Walterová
2
Walterová
2
2
2
Cibiček
Cibiček
Walterová
2
Ulrichová
2
2
Walterová
8 04.11.2015 Glycoproteins. Glycosaminoglycans.
Proteoglycans.
05.11.2015 Osteosynthesis. Extracellular matrix - proteins.
9 11.11.2015 Biochemistry of liver.
12.11.2015 Metabolism of xenobiotics.
10 18.11.2015 Gastrointestinal digestion and absorption.
19.11.2015 Biologically important radicals. Oxidative stress.
Antioxidants.
11 25.11.2015 NO - Biosynthesis and function.
26.11.2015 Biochemistry of muscle contraction – energy
supply in skeletal and muscle heart.
12 02.12.2015 Nucleotides-structure, function, metabolism.
03.12.2015 Nucleic acid – analysis.
13 09.12.2015 Biochemistry of neurodegenerative diseases.
Biochemistry of vision.
10.12.2015 Minerals, trace elements – metabolic and biological
role.
14 16.12.2015 Biochemical markers in clinical diagnosis I.
17.12.2015 Biochemical markers in clinical diagnosis II.
15 06.01.2016 Biochemical markers in clinical diagnosis III.
07.01.2016 Overview of metabolic pathways.
2
Vostálová
2
2
2
2
2
Walterová
Cibiček
Cibíček
Cibiček
Ulrichová
2
Cibiček
Walterová
2
2
2
2
Ulrichová
Ulrichová
Ulrichová
2
Cibiček
2
2
2
2
Cibiček
Cibiček
Cibiček
Ulrichová
Exercises :
Leading Teacher :
Mgr. Pavel Kosina, Ph.D.
Study :
Continuous
Week from-to
1
2
3
4
5
6
Subject
14.09. – 18.09.2015 Information sources, databases. Training of laboratory
skills. Blood collection systems.
21.09. – 25.09.2015 Enzymes I.
Demonstration of substrate specificity of -amylase and
sucrase.
Determination of optimum temperature for trypsin.
28.09. – 02.10.2015 Acid-basic equilibrium.
28.09. state holiday
05.10. – 09.10.2015 Enzymes II.
Determination of Michaelis constant of alkaline
phosphatase (ALP) in serum.
Inhibition of catalase activity.
12.10. – 16.10.2015 Enzyme III.
Determination of lactate dehydrogenase (LD) aspartate
aminotransferase (AST) and alanine aminotransferase
(ALT) activity in serum.
19.10. – 23.10.2015 Immunoanalytical methods.
No. of
less.
4
4
4
4
4
4
7
8
9
10
11
12
13
14
15
Point of care testing.
Determination of specific antigen by ELISA method.
Determination of rheumatoid factor in serum by Latex
agglutination test.
26.10. – 30.10.2015 Revision of key metabolic pathways.
28.10. state holiday
02.11. – 06.11.2015 Metabolism of saccharides.
Determination of glucose in blood and urine (Oral glucose
tolerance test).
Determination of glucose in blood by glucometer.
Determination of glucose and ketones in urine by
diagnostic test strips.
Determination of glycated hemoglobin.
09.11. – 13.11.2015 Lipid metabolism.
Determination of diagnostically important atherosclerosis
parameters in serum (total cholesterol, HDL-cholesterol,
LDL-cholesterol, triglycerides).
16.11. – 20.11.2015 Chapters from biochemistry (presentation).
17.11. state holiday
23.11. – 27.11.2015 Amino acids metabolism. Digestion of proteins.
Determination of creatinine in serum and urine.
Determination of cystatin C in serum.
Clearance and glomerular filtration calculations.
Semi-quantitative determination of albuminuria.
30.11. – 04.12.2015 Tetrapyrrols metabolism.
Determination of hemoglobin in blood.
Determination of bilirubin in serum.
Test for blood in faeces and urine.
Gallstone analysis.
07.12. – 11.12.2015 Analysis of gastric juice. Microscopy of urinary
sediment, urinalysis.
Urinalysis, detection of selected parameters in urine.
Determination of gastric output.
14.12. – 18.12.2015 Practical test.
04.01. – 08.01.2016 Revision of key metabolic pathways.
Completed by :
Requirements :
Literature :
4
4
4
4
4
4
4
4
4
Course unit credit
100% presence in practical lecture; obtain at least 70 % points in
continuous test/or at least 70 % points in course unit credit test
on principles of methods and calculations in chemistry; presentation of
one selected biochemical topic; pass the final practical test
Champe P. C., Harvey R.A.: Biochemistry (6th ed.), Lippincott
Williams & Wilkins 2013.
Devlin T.M. Textbook of biochemistry with clinical correlations (7th
ed.), John Wiley & Sons, Inc. 2010.
Murray R.K., Bender D.A., Botham K.M., Kennelly P.J., Rodwell V.W.,
Weil P.A. Harpers Illustrated Biochemistry (29th ed.), Lange Medical
Book 2012.
Koolman J. Color Atlas of Biochemistry (3rd ed.), Georg Thieme Verlag
2012
Newsholme E., Leech T. Functional Biochemistry in Health and Disease
(2nd ed.), Wiley-Blackwell, 2010
Dvořáčková S., Dvořák Z., Valentová K., Vičar J. Biochemistry.
Laboratory classes. 2007.