AP Biology Chapter 19 Assignment
After reading this chapter and attending lecture, you should be able to:
1. Explain how advances in recombinant DNA technology have helped scientists study
the eukaryotic genome.
2. Describe the natural function of restriction enzymes.
3. Describe how restriction enzymes and gel electrophoresis are used to isolate DNA
fragments.
4. Explain how the creation of sticky ends by restriction enzymes is useful in producing
a recombinant DNA molecule.
5. Outline the procedures for producing plasmid and phage vectors.
6. Explain how vectors are used in recombinant DNA technology.
7. List and describe the two major sources of genes for cloning.
8. Describe the function of reverse transcriptase in retroviruses and explain how they are
useful in recombinant DNA technology.
9. Describe how "genes of interest" can be identified with the use of a probe.
10. Describe how bacteria can be induced to produce eukaryotic gene products.
11. List some advantages for using yeast in the production of gene products.
12. Describe some practical applications of recombinant DNA technology in biological
research.
13. List and describe four complementary approaches used to map the human genome.
14. Explain how RFLP analysis and PCR can be applied to the Human Genome Project.
15. Describe how recombinant DNA technology can have medical applications such as
diagnosis of genetic disease, development of gene therapy, vaccine production, and
development of pharmaceutical products.
16. Describe how gene manipulation has practical applications for agriculture.
17. Describe how plant genes can be manipulated using the Ti plasmid carried by
Agrobacterium as a vector.
18. Describe how recombinant DNA studies and the biotechnology industry are regulated
with regards to safety and policy matters.