Cell Culture BTC 285 (2-3-4)
Instructor: Dr. Sandol Johnson
Fall 2012
Class Time: Tuesday and Thursday 1:00 to 3:45 PM
Bailey Room 5332
Phone: 704-669-4125
E-mail: johnsons@clevelandcc.edu
Office: Bailey 5329
Office Hours: Office Hours: 1:00 to 3:00 PM Mon, 1:00 PM to 2:30 PM Wed, 12:00 PM to 1:00 PM Fri
I. Course Description: This course introduces the theory and practices required to successfully initiate and maintain plant
and animal cell cultures. Topics include aseptic techniques, the growth environment, routine maintenance of cell cultures,
specialized culture techniques, and various applications. Upon completion, students should be able to demonstrate the
knowledge and skills required to grown, maintain, and manipulate cells in culture. Prerequisites: BIO 175 or BIO 275
II. Required Text Materials:
Text: Biotechnology, Science for the New Millennium by Ellyn Daughterty, Latest Edt. ISBN: 978-0-76384-284-0,
Paradigm Publisher
Lab Manual: Biotechnology Laboratory Manual by Ellyn Daughterty, Latest Edt., ISBN: 978-0-76384-286-4,
Paradigm Publisher
Reference books (not required):
Culture of Animal Cells, A Manual of Basic Technique by R. Ian Freshney ISBN 0-471-45329-3
5th Edt., Publ.: John Wiley & Sons, Ltd.
Basic Cell Culture, A Practical Approach, Edited by J. M. Davis, ISBN13: 9780199638536
ISBN10: 019963853, 2nd Edt., Publ. IRL Press at Oxford University Press., Oxford.
(http:// NowDownloadall.com)
Computer: Ability to hear and see videos online on Blackboard including www.youtube.com
Lab Manual: “Composition Book,” notebook to keep lab reports.
III. Course Objectives:
 To introduce students to the theory and practices of cell culture.
 To understand the processes in plant and animal cell culture.
 To understand the aseptic technique.
 To introduce students to the proper techniques in cell growth and maintenance.
 To study various materials and media used in cell growth and maintenance.
 To gain knowledge in manipulation of cells in culture.
IV. Methods of Instructions:
 Online notes and lectures.
 Power Point presentations online.
 Various videos from company sources.
 Objective and discussion quizzes to reinforce learning.
 Demonstration of various lab techniques.
 Hands on labs for students to increase their confidence and skills.
V. Learning Activities:
 Discuss the history of tissue culture.
 Discuss the importance of tissue culture to biotechnology.
 Observe and practice aseptic technique.
 Observe and practice plant cell and tissue culture.
 Observe and practice animal cell and tissue culture.
BTC 285, F2012
Johnson
Page 1
VI. Critical/Analytical Thinking Skills:
 Analyze how tissue and cell culture is used in industry.
 Consider the importance of aseptic technique.
 Analyze the proper techniques and their outcomes.
 Critique the final outcomes of the lab assignments.
VII. Student Learning Outcomes: Upon completion of this course, the student will be able to:
 Students should understand the history and future of cell culture.
 Students should familiar with aseptic techniques.
 Students should be able initiate and maintain plant cell cultures.
 Students should be able initiate and maintain animal cell cultures.
 Students should be able to maintain proper environment for cell growth.
 Students should be knowledgeable in specialized cell techniques.
VIII. Student Requirement:
 Visit the Blackboard site twice weekly
 Review the assigned Power Points and extra material for that week
 Attend all scheduled labs, remember this is an 8 week course. Each absence will deduct 10% from your
Final Attendance grade
 Be prepared before coming into the lab
 Complete all assignments for the week on time
IX. Evaluation:




Weekly online quizzes………....30%
Attendance—Lab and Online.....20%
Lab Notebooks………………....30%
Final Project…………………....20%
X. Attendance Policy/Make-up Policy:
Since this is an 8 week Hybrid class, you are expected to keep up with the Blackboard material and be in the scheduled lab
days. The labs will be very hard to make up. Under EXTREME situations, we will discuss your options.
Inclement Weather: Check the college website for closing and then check the Blackboard for assignment.
XI. Academic Honesty Policy:
Cleveland Community College expects students to practice academic honesty at all times. Refer to the Academic Honesty
Policy under Blackboard that is taken from pages 23 in the Academic Bulletin & Student Handbook, 2010-2011.
XII. Grading Scale:
A
B
C
D
F
I
93-100
85-92
77-84
70-76
Below 70
Incomplete
Important Dates:
August 16
September 3
October 1-6
November 15
November 22-24
December 17
BTC 285, F2012
Johnson
Class begins
Labor Day (No Class)
Fall Break (No Class)
Last Day for Official Withdrawal/Audit
Easter Break
Last Day of Fall Semester
Page 2
WEEK
1
2
3
4
5
6
7
8
9
10
11
12
PROJECTED WEEKLY SCHEDULE
Introduction to Cell and Tissue Culture
Tissue Culture Facilities and Safety
Microscope, Cell Count, Aseptic Technique Labs 2C-D
Quiz online
Continuation of Microscope, Cell Count, Aseptic Technique
Quiz online
Cells and Microscopes and Sterile Technique
Cell Structures, Labs 2A-B
Quiz online
Cell Structures Labs 2 E-F
Quiz online
Labs 2C-D
Comparisons of plant tissue and animal cell
Quiz online
Cell comparisons
Quiz online
Plant Cloning and Propagation Labs 10 A, B, and C
Quiz online
Plant Cloning and Propagation Lab 11
Quiz online
Plant Cloning and Propagation Continuation of Lab 11
Cell Cycle and Apoptosis
Quiz online
Review videos and prep for Animal Cell Line Propagation
Quiz online
Animal Cell Lines and Propagation
Media Preparation, Aseptic Techniques, Cell Counting
Quiz online
Animal Cell Lines and Propagation
Media Preparation, Aseptic Techniques, Cell Counting
Prepare for Final Project
Quiz online
13
Animal Cell Lines and Propagation
Media Preparation, Aseptic Techniques, Cell Counting
Prepare for Final Project
14
Work on Final Project
15
Final Project Analysis, Turn in Lab Notebooks
16
Clean up Lab, review procedures
BTC 285, F2012
Johnson
Page 3