Product Datasheet
Anti-PKAc alpha/beta/gamma antibody - C-terminal
ab211265
2 Images
Overview
Product name
Anti-PKAc alpha/beta/gamma antibody - C-terminal
Description
Rabbit polyclonal to PKAc alpha/beta/gamma - C-terminal
Specificity
ab211265 recognizes endogenous levels of PKAc alpha/beta/gamma protein.
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig
Immunogen
Synthetic peptide within Human PKAc alpha/beta/gamma (C terminal) conjugated to Keyhole Limpet
Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P22694
Positive control
HEK293T and mouse NIH 3T3 whole cell lysates; mouse lung and rat lung lysates; Human brain tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid
freeze / thaw cycle.
Storage buffer
pH: 7.3
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab211265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with
IHC staining protocol.
WB
1/500 - 1/1000. Predicted molecular weight: 41 kDa.
Target
Relevance
PKA alpha, beta and gamma are members of the Ser/Thr protein kinase family and are a catalytic subunit of
cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP
exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through
phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two
regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of
regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
Cellular localization
Cytoplasmic and Nuclear
Anti-PKAc alpha/beta/gamma antibody - C-terminal images
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Product Datasheet
All lanes : Anti-PKAc alpha/beta/gamma antibody - Cterminal (ab211265) at 1/500 dilution
Lane 1 : HEK293T whole cell lysate
Lane 2 : mouse NIH 3T3 whole cell lysate
Lane 3 : mouse lung lysate
Lane 4 : rat lung lysate
Western blot - Anti-PKAc alpha/beta/gamma antibody
- C-terminal (ab211265)
Predicted band size : 41 kDa
Immunohistochemical analysis of formalin-fixed, paraffinembedded Human brain tissue labeling PKAc
alpha/beta/gamma with ab211265 at 1/100
dilution. Detected utilised an HRP conjugated compact
polymer system. DAB was used as the chromogen. The
section was then counterstained with haematoxylin and
mounted with DPX.
Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - Anti-PKAc alpha/beta/gamma
antibody - C-terminal (ab211265)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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Terms and conditions
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Visit us at: www.abcam.com
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