RIZAL TECHNOLOGICAL UNIVERSITY
Boni Avenue, Mandaluyong City, Philippines
COLLEGE OF ARTS & SCIENCES
COURSE SYLLABUS
Subject Code:
Section Title:
Credit:
Semester:
School Year:
Professor:
Biotechnology 2
PLANT PHYSIOLOGY & IN VITRO CULTURE
4 Units
1st & 2nd Semester
2008 – 2009
PROF. NORBERTO R. BAUTISTA
I. Course Description:
This course is a study of physiology and growth of plants as a prerequisite in
gaining the skills in plant tissue culture. It involves a deeper look into the chemical
processes in plants like photosynthesis, respiration, nutrition, protein and hormone
synthesis, and metabolism. The course also studies embryogenesis, seed maturation,
and germination, effect of plant growth regulators on plant growth, photoperiodism and
the transformation of vegetative stage to reproductive stage in plants. The study of plant
physiology is an effort to explain the concepts behind plant tissue culture and the
application of the subject to the plant industry.
Time:
Prerequisites:
College Offering the Subject:
3 Hrs Lecture; 3 Hrs Laboratory per week
CHEMISTRY 4 & BOTANY 1
CAS
II. Goal
To provide the students with some valuable insights on the deeper concept of
plant physiology in an effort for man to control plant growth in relation to plant tissue
culture and also in plant production as a whole.
III. Subject Objectives
At the end of the semester, students are expected to:
1. to motivate the students in studying plant physiology by providing vivid
introduction about the subject as a course;
2. to provide understanding of the nature and characteristics of plants; and
3. to understand the effect of the physical environment and genetics on plant
growth and how to simulate this environment for controlling plant growth and
reproduction for man’s benefit.
IV. Instructional Modes
A. Traditional Experiences: Lecture, Discussions, Special Report and
Demonstration through the use of blackboard / whiteboard, overhead
projector, Powerpoint presentation, hand-outs, the internet, LCD, VCD, DVD
and / or other instructional materials.
B. Laboratory Work: Hands-on experience in the laboratory on media
preparation, sterilization technique, inoculation, and nursery management.
C. Field Trip: Visitation to commercial and government plant tissue culture
laboratories and plant nurseries.
V. Subject Content.
No. Hrs.
Unit 1 - Concepts in Plant Physiology
Module 1 – Introduction to Plant Physiology / Plant tissue culture & Its
Application in Agriculture & Biotechnology
Module 2 -- History of Plant Physiology & Plant tissue culture
Module 3 – Foundation Concepts of Plant Tissue Culture
3.0
3.0
1.5
Unit 2 - A Deeper Look on Plants
Module 4 -- The Plant Cell & Tissue Types
Module 5 – Leaves & their Modifications
Module 6 – Plant Roots
Module 7 – Stems
Module 8 – Flowers
Module 9 – Fruits
Module 10 – Seeds
3.0
1.5
1.5
1.5
1.5
1.5
1.5
Unit 3 - Physiological Processes in Plants
Module 11 – Photosynthesis and Nutrient Transport
Module 12 -- Sink and Source relationships
Module 13 – Plant Respiration
Module 14 – Plant Nutrient Requirements & Absorption
Module 15 – Plant Maturation: Vegetative & Reproductive tendencies
Module 16 – Influence of Photoperiodism, Gravitopism and others
Module 17 -- Formation of Storage Organs
Module 18 – Embryogenesis
Module 19 – Dormancy in Plants
Module 20 -- Plant Reproduction
3.0
1.5
3.0
3.0
1.5
1.5
1.5
1.5
1.5
3.0
Unit 4 - Plant Growth Regulators (PGR): Their Biosynthesis, Transport & Action
Module 21 – Auxins
Module 22 – Cytokinins
Module 23 – Gibberellins
Module 24 -- Abscisic acid
Module 25 – Ethylene
Module 26 -- Other forms of PGR’s
1.5
1.5
1.5
1.5
1.5
1.5
Unit 5 - Concepts in Plant Tissue Culture
Module 27 – General Introduction to Plant Tissue Culture, Its History,
Botanical Basis, Uses, Advantages and Disadvantages.
Module 28 -- The Culture Medium
Module 29 -- The Plant Tissue Culture Laboratory
Module 30 -- Laboratory Practices & Techniques
Module 31 -- Costing in Establishing a PTC Laboratory
Module 32 – Applications of PTC in Agriculture & Forestry
*Field Trip to Laboratories & Plant Nurseries
3.0
3.0
3.0
3.0
3.0
3.0
VI. Course Requirements:
1. 3 Major Exams
2. Laboratory Exercises
3. 1 Special Report
VII. Grading System.
Lecture…………………………………….50%
Quizzes………………10%
Assignments…………10%
Exams………………..25%
Attendance……………05%
Laboratory…………………………………50%
Quizzes………………05%
Assignments…………20%
Exams………………..20%
Attendance……………05%
TOTAL:
100%
E. Bibliography
Biotechnology by Open Learning (BIOTOL) (1993) In Vitro Cultivation of Plant
Cells. Butterworth-Heinemann Ltd., London, UK.
Dizon, R.A. and Gonzales, R.A. (eds.) (1994) Plant Cell Culture: A Practical
Approach. Oxford University Press, New York.
Endress, R. (1994) Plant Cell Biotechnology. Springer-Verlag Berlin Heidelberg,
Germany.
Galbriath, D.W., Bohnert, H.J. and Bourque, D.P. (eds.) (1995) Methods in Plant
Cell Biology: Part A. Vol. 49. Academic press, Inc., California.
Hall, R.D. (ed.) (1999) Plant Cell Culture Protocols. Human Press, Totowa,
New Jersey.
Kyte, L. (1987) Plants From Test Tubes: An Introduction to Micropropagation.
Timber Press, Portland, Oregon.
Pierik, R. (1997) In Vitro Culture of Higher Plants. Academic Publishers,
London.
Rickwood, D. and Hames, B. (ed) (1994) Plant Cell Culture: A Practical
Approach. Oxford University Press, New York.
Weier, T., Stocking, C., Barbour, M. and Rost, T. (1982) Botany: An Introduction
to Plant Biology. John Wiley and Sons, New York.
Noted by:
Prepared by:
DR. MERLENE BERNAL
Dean, CAS
NORBERTO R. BAUTISTA
Instructor, BIOTECH 2
Date Submitted: 06-23-2008
Revised: 06-23-2008