Cell Membrane

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Ch 1 - The Cell & Cell Processes
The Cell
P All cells have
< Cell membrane - phospholipid bilayer surrounding cell parts
< Cytoplasm - everything between cell membrane + nucleus
P Most cells have
< Nucleus - “brain” of the cell
< Contains DNA (instructions)
P Prokaryotes
< Have no nucleus in cell(s)
< Ex. Bacteria, red blood cells
P Eukaryotes
< Have well organized nucleus in cell(s)
< Ex. Most plant and animal cells
Nucleus
Site of DNA replication + transcription
P Nuclear membrane
< Membrane surrounding nucleus, has pores
< Controls what can enter or leave nucleus
P Nucleoplasm
< Jelly-like material inside nucleus
< Helps give nucleus shape, support
P Nucleolus (or nucleoli)
< Dense body rich in rRNA + subunits
< Involved in ribosome production
P Chromosomes (chromatin form)
< Each = protein core with DNA wrapped around it
< Contains instructions for cells; genes code for proteins ~ traits
Cell Membrane
“Selectively permeable phospholipid bilayer”
P Selectively permeable
< Controls what can enter and leave cell
P Phospholipid bilayer
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Phospholipids are special fats
Hydrophobic tails, hydrophilic heads
Arrange so protect hydrophobic parts
Tails go inside, heads twd water
Several proteins embedded in it
Cholesterol also embedded ~ rigidity
P “Fluid Mosaic”
Hydrophilic Head
Hydrophobic Tails
Membrane Proteins
embedded in phospholipid bilayer
P Signature molecules (glycoproteins)
< have sugar part that sticks out for identification/recognition of cell
P Gatekeepers
< Allow only certain materials to enter or leave cell
P Receptor sites
< Hormones bind to receptor and initiate cell response
P Enzymes
< Proteins that act as catalysts for reactions (speed up)
P Transport proteins (move substances across membrane)
< Active transport - against [ ] gradient; requires ATP
< Passive transport - down [ ] gradient; no ATP needed
Cytoplasm
P Contains:
< Water, nutrients, wastes
< Building blocks of materials needed by cell
< Organelles
P Organelles
< “small organs” that work together to keep cell alive
< Each type has a special function
< Note: some differences between plant and animal cells
Organelles
P Mitochondria (mitochondrion, s)
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“powerhouse of the cell”, oval shaped
Double membrane, smooth outer
Folded inner membrane
Folds (cristae) allow for more SA
Site of cellular respiration (food þ energy)
P Ribosomes
< “protein factory”, site of protein synthesis
< Reads message (mRNA) from nucleus
and puts amino acids in proper order
< Some free in cytoplasm, some stuck on ER
Organelles
P Endoplasmic reticulum
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Double membrane channel
Transports materials through cytoplasm
Smooth: no ribosomes attached (~ fats)
Rough: ribosomes attached (~ proteins)
P Golgi Body (Golgi Apparatus)
< “processing & packaging factory”
< Processes and packages into vesicles
< Contents sent to membrane or out of cell
P Lysosomes
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“suicide sacs”, small round bodies containing enzymes
fuse with “old cell parts” to break them down and recycle parts
fuse with foreign particles to destroy them (ex. in WBCs)
fuse with food vacuoles to help break down nutrients
Organelles
Cytoskeleton Parts
P Microfilaments
< Pipelike structures made of proteins (actin, myosin)
< Imp for structure + mvmt, changes in cell shape
< Ex. Muscle contraction, ameboid mvmt
P Microtubules
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Hollow cylinders made of spherical protein, tubulin
Transport materials through cytoplasm
Part of cytoskeleton, helps give cell shape
Involved in movement of cell (cilia or flagella)
– Cilia - tiny hairlike structures that aid movement (oars)
– Flagella - longer whiplike structures that propel cell forward
Organelles
P Vacuoles
< Storage spaces within cell for food, water, or wastes
< Animals have a few small ones
< Plants have very large central one for water
P Centrosome
< “Microtubule organizing center”
< Contains centrioles in animal cells
< Involved in spindle formation during cell division Centrioles
P Centrioles
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Chromosome
Only in animal cells
Composed of microtubules
Involved in spindle formation during cell division
Work with spindle to pull chromosomes apart during division
Special Plant Cell Features
P Cell Wall
< Outside layer provides support, protection, shape
< Composed of cellulose (provides roughage for us)
P Plastids
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Associated with production and storage of food
Usually contain pigments
Cells reflect the color of the pigment they contain
Chromoplasts - store pigments; yellow, orange, blue, red
Amyloplasts - store starch; no pigment so colorless
P Chloroplasts (another plastid)
< site of photosynthesis; contain chlorophyll (green pigment)
< Chlorophyll traps energy from sun for photosynthesis
< Grana = stacks of membranes that increase SA to trap light
Animal Cells vs Plant Cells
Animal Cells
Plant Cells
1. No cell wall
1. Have a cell wall
2. Few, v small vacuoles
2. Large central vacuole
3. Centrosome with centrioles
3. No centrosome or centrioles
4. No plastids
4. Have plastids (ex. Chloroplast)
5. No crystals
5. Have crystals (raphides, druse)
6. Store carbs as glycogen
6. Store carbs as starch
7. Can change their shape
7. Have fixed, rectangular shape
Animal Cell
Pinocytotic vesicle
Lysosome
Golgi vesicle
Mitochondrion
Golgi Body / Apparatus
Nucleolus
Rough Endoplasmic Reticulum
Smooth Endoplasmic Reticulum
Centrosome
Vacuole
Cell Membrane
Nucleoplasm
Nuclear Membrane
Centriole
Microtubule
Cytoplasm
Ribosome
Nucleus
Plant Cell
Golgi vesicle
Ribosome
Smooth Endoplasmic Reticulum
Nuclear Membrane
Nucleolus
Nucleus
Nucleoplasm
Rough Endoplasmic Reticulum
Cell Wall
Cell Membrane
Golgi Body / Apparatus
Chloroplast
Granum (grana, pl)
Raphide Crystal
Central Vacuole
Amyloplast
Druse Crystal
Mitochondrion
Cytoplasm
Cell Processes
P Passive Transport
< Diffusion
< Osmosis
< Solutions: hypotonic, hypertonic, isotonic
P Active Transport
< Transport proteins
< Endocytosis (Phagocytosis, Pinocytosis)
< Exocytosis
Passive Transport
Diffusion
P Description
< Mvmt of substance from hi [ ] / pressure to low [ ] / pressure
P Example
< O 2 diffuses fr blood (8 [ ] ) to tissues (lo [ ] ), CO 2 opposite
Passive Transport
Theory Behind Diffusion
P Brownian motion
< Mculs move about randomly and collide
< Collisions force molecules to spread apart (to fill an area)
P Factors that affect rate of diffusion
< Temperature - 8 temp, 8 speed of mculs, 8 collisions, 8 rate
< Concentration - 8 [ ] , 8 # particles colliding, 8 collisions, 8 rate
< Pressure - 8 pressure, 8 bunched tog, 8 collisions, 8 rate
Passive Transport
P Facilitated Diffusion
< Specialized carrier proteins aid and speed up mvmt of
certain mculs across membrane
< Ex. Glucose diffusion across membranes
Diffusion through Channel
Facilitated by Carrier Protein
Passive Transport
Osmosis
P Description
< Mvmt of water mculs fr area of hi [ ] to low [ ]
< Note: mvmt of WATER molecules not solute particles
< Misconception: easy to confuse with diffusion (mvmt of solute)
Ž water mcul
Ž solute mcul
Osmosis = mvmt of Ž fr hi to low [ ]
Passive Transport
Osmosis
P Solution Descriptors
< Hypertonic solution - has more solute than the one being referred to
< Hypotonic solution - has less solute than the one being referred to
< Isotonic solution - has the same amount of solute
Which Way Will H 2O Move?
Solute particle
Cell
Container of solution
Osmosis
H2 O moves in, cell swells
(In plant cells, causes turgor pressure)
(In animal cells, may burst = cytolysis)
Cell in hypotonic solution
Osmosis
Cell in hypertonic solution
H2 O moves out, cell shrinks (plasmolysis)
Active Transport
“Capture and Release”
P Theory
< In some cases, transport proteins capture or bind
solute mculs with proper shape and move them
across the cell membrane
Active Transport
Pumps
P Theory
< Some transport proteins actively pump substrates
across membrane
< Ex. Sodium-potassium pumps in nerve cells use
energy to pump Na+ and K+ to proper sides of nerve
cell membrane for nerve to fire
< 3 Na+ out for every 2 K+ in
Active Transport
Endocytosis
PGeneral Definition
< Process by which materials too large to fit through pores or
channels in membrane are moved from outside to inside cell
PPinocytosis
< Cell membrane pinches inward forming vesicle
< Brings in liquid material (sm particles)
PPhagocytosis
< Cell membrane extends around large object
< Forms vesicle as brings material in
< Ex. Amoeba feeding
< Ex. WBC capturing bacteria
Active Transport
Exocytosis
P General Definition
< Process by which large materials within the cell are transported to the
outside of the cell
< Materials are carried inside vesicle to cell membrane
< Vesicle fuses with membrane and releases contents
< Ex. Removal of wastes, secretion of proteins, neurotransmitter release
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