BIO 226
JOHN A. LOGAN COLLEGE
J. Forer – SP 14
GENERAL MICROBIOLOGY
4 cr. (2-4)
COURSE DESCRIPTION:
An introduction to the study of microorganisms, including their morphology, physiology,
cultivation, classification, pathogenicity, economic importance, control, and immunity.
Laboratory experiments guide students in development of laboratory procedures, sterile
techniques, and data interpretation.
PREREQUISITES: None
COURSE OBJECTIVES:
The students should learn to:
1.
Identify and apply key concepts outlined by the American Society for
Microbiology’s Curriculum Recommendations: Introductory Course in
Microbiology. http://www.asm.org/Education/index.asp?bid=10051
a. Microbial cell biology, genetics, evolution and diversity
b. Interactions and impact of microorganisms and humans
c. Interactions and impact of microorganisms in the environment
2.
Explain and practice safe microbiological procedures.
3.
Carry out the following laboratory procedures from the core lab skills portion of
the ASM’s Curriculum Recommendations: Introductory Course in Microbiology.
a. Use a bright field light microscope to view and interpret slides
b. Properly prepare slides for microbiological examination
c. Properly use aseptic techniques for the transfer and handling of
microorganisms and instruments
d. Use appropriate microbiological media and test systems
e. Estimate the number of microbes in a sample using serial dilution techniques
f. Use standard microbiology laboratory equipment correctly
4.
Practice the following scientific activities processes:
Cognitive processes, including:
a. predicting expected results
b. following an experimental protocol
Analysis skills, including:
a. Collecting and organizing data in a systematic fashion
b. Presenting data in an appropriate form
c. Drawing appropriate conclusions based on the results
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Communication skills, including:
a. Discussing and presenting lab results or findings
Interpersonal and citizenry skills, including:
a. Working effectively in teams or groups so that the task, results, and analysis
are shared
b. Integrating knowledge and making informed judgments about microbiology in
everyday life
5.
Manipulate and interpret quantitative and qualitative information relating to
microbial numbers and microbial identification. Relate collected or observed
results to reference material in graphic or tabular form. Construct graph and
tabular representations of results.
6.
Read and summarize microbiological information from several different sources.
Identify reliable sources of microbiological information.
COURSE OUTLINE:
Lecture: Chapters refer to Chapters in Tortora, Funke and Case
Week 1
Week 2
Week 3
Week 4
Week 5
Chapter 1
Chapter 2
Chapter 4
Chapter 14
Exam 1;
start Chapter 5 –
Week 6
Chapter 5
Week 7
Chapter 7
Week 8
Chapter 13
Week 9
Exam 2;
start Chapter 8 –
Week 10
Chapter 8
Week 11
Chapter 9
Week 12
Chapter 20
Week 13
Exam 3;
Chapter 15
Chapter 16
Week 14
Chapter 10
(in lab only) Chapter 12
The Microbial World and You
Chemical Principles
Functional Anatomy of Cells
Principles of Disease and Epidemiology
Microbial Metabolism
Microbial Metabolism
Control of Microbial Growth
Viruses, Viroids and Prions
Microbial Genetics
Microbial Genetics
Biotechnology and Recombinant DNA
Antimicrobial Drugs
Mechanisms of Pathogenicity
Nonspecific Defenses
Classification of Microorganisms
The Eukaryotic Microbes: Fungi, Algae, Protozoa, and
Helminths
Exam 4
Week 14
Chapter 17
Specific Defenses
Chapter 18
Practical Applications of Immunology
Week 15
Chapter 19
Disorders of the Immune System
Exam 5
Final during Finals week at scheduled time.
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Laboratory Schedule:
Week
1 (1/13)
(V) Microbial Universe
2 (1/20)
(V) Disease Warriors
3 (1/27)
(V) Plague Fighters
(V) Deadly Messengers
(Tuesday only)
4 (2/03)
(V) Microbial Ecology
First article summary
due
5 (2/10)
(V) Enzymes
6 (2/17)*
(V) Microbial Evolution
video
(V) H5N1 flu video
7 (2/24)
(V) Microbial
Metabolism
8 (3/03)
(V) Superbugs video
9 (3/17)
(V) Control of Microbial
Growth
(V) Microbial Gene
Transfer
10 (3/24)
(V) Gene Expression
(V) HIV Drugs (Holliday
lecture)
Activity
Microbiology laboratory instructions
Data Interpretation
A food poisoning outbreak
Use and Handling of the microscope Flu simulation
Aseptic technique and introduction to Dermatitis
culture media
Observing microbial growth in lab
media
Staining bacteria for microscopy:
Heat fixed smears and simple stains
Gram staining
Purification of bacteria by plate
streaking
Gram staining
Use of light microscope to
examine live bacteria and protists
Scientific notation
Measurement of bacterial numbersstandard plate count
Dilutions and plate counts
Metabolism I - Selective and
differential media, Enzyme tests
Factors that affect bacterial growth
Bacterial growth and growth curves
Scientific notation and
dilutions
Yogurt production
ELISA
Oxygen questions
Lab Exam 1 (second day)
Bacterial transformation: Control of
microbes by heat, radiation, and
Hand washing:
Use-dilution test
Kirby-Bauer technique
Chicken marinade
Multitest system and bacteria of the
intestine
Plate count problems
Microbial growth activities
Fibronectin and Biofilm
formation
Gene expression activity
Antibiotics and drug
resistance in the
microbiota
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Week
11 (3/31)
(V) Universe Within
(Mountain Climber)
(V) Cell Wars
12 (4/07)
(V) Microbes and
Disease
(V) How Safe Are We?
Second article
summary due
13 (4/14)
(V) Gene technology
(V) Eating Us Alive
14 (4/21)
Activity
Bacteriophage
Begin work on unknown
Data Interpretation
Flowchart practice
Work on unknown
Microbiota of skin and throat
Wed. classes, PCR
PCR and DNA
Electrophoresis
Finish unknown work
Classification and Eukaryotic
pathogens
15 (4/28)
(V) Surviving AIDS
* - Indicates No Monday classes
Iron Microarray analysis
Lab Exam 2
METHOD OF EVALUATION:
To succeed in the course, students will need to:
1. Fulfill their responsibilities as a student as described by the college publication
Student Rights and Responsibilities, generally available at Admissions and major
college entrances.
2. Demonstrate competence in laboratory safety and techniques.
3. Perform satisfactorily on quizzes, exams and other assignments.
Grading:
Lecture exams .................................................40%
Final exam (over lecture).................................15%
Lab exams (2) .................................................15%
Lecture attendance and quizzes/activities .......15%
Lab attendance and quizzes/activities .............15%
Final grades will be determined as follows:
90% or more ........................................................A
80 - 89% ..............................................................B
70 - 79% ............................................................ C
60 - 69% ............................................................. D
Less than 60% ....................................................E
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Your instructor will strive to assist you in mastering course material and laboratory skills,
but the responsibility for your academic success must rest primarily on your
shoulders. It is your responsibility to seek help when you need it, to maintain a realistic
work and class load for your life circumstances, and to find sufficient time outside of
class to prepare adequately. You are likely to need 6-8 hours a week of study and
classwork time in addition to your in-class time for this class (based on the standard of 2
hours study/hour of lecture and 1 hour study/hour of lab typical for a college course). If
you are unable to commit this amount of time, your performance in the class may suffer.
Chronic tardiness is disruptive to the rest of the class, may lead to lab safety problems
and will not be tolerated.
Regular attendance in both lecture and lab is required for successful completion of the
class; this is both a college policy and a policy for this particular class. A significant
portion of your grade depends on your presence in the classroom and laboratory. Your
instructor may not permit you to return to class after 3 consecutive absences or 5
accumulated absences. Please contact your instructor promptly if you must miss class;
it may be possible to arrange to make up work with another section to reduce the impact
on your education and your grade.
CLASS DECORUM
You are entitled to expect civility and respect from your fellow students and instructor
throughout the semester, and you are expected to provide the same courtesies to them.
Please be considerate of fellow students and your instructor, particularly during lecture.
Walking in or out of the room, having conversations unrelated to class with other
students when the instructor is talking, or using electronic devices during lecture are
examples of disruptive behaviors that make it hard for others to concentrate and learn.
If you must leave the room to deal with an emergency or illness, I expect you to do so,
but otherwise, respect the needs of others in the classroom to have an environment free
of distractions.
Electronic Equipment
ALL use of electronic devices (phones portable audio devices, texting devices, laptop
computers and other electronic equipment) during class is prohibited. Please keep any
devices turned OFF and put AWAY except during breaks. Video and audio recording of
lectures may be done only with consent of the instructor.
If you have special circumstances that require an exception to this policy, including use
of a laptop computer for notetaking and/or a recording device for recording lectures,
please meet with your instructor to obtain permission for limited use of the equipment in
a way that will not disturb other students. If permission is granted to record lectures, it
will be on the condition that these recordings are only used as a study aid by the
individual making the recording. Permission is withdrawn if you violate the terms of use
agreed upon by your instructor.
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Your instructor may have other more specific policies about the use of electronics
during class. They will be consistent with John A. Logan College’s Student Rights and
Responsibilities Handbook.
Part of the purpose of this class is to allow students to develop and practice safe lab
habits. Your instructor will talk to you if this becomes a problem. A student who
demonstrates that he or she is unwilling or unable to work safely in the laboratory will be
removed from lab until the problem can be resolved to the instructor’s satisfaction. The
first concern of the instructor is for the safety of all students and persons at the college.
The student may not be able to make up the grades missed during his or her absence
from lab.
A student who is in repeated violation of classroom policies or violates John A. Logan
College’s Student Code of Conduct (Rights and Responsibilities publication), may be
removed from class until he or she meets with the instructor and, if necessary, other
college personnel to resolve the problem. Written and verbal notice of the violation will
be given to the student at the time of removal, if possible.
Your grade is affected by lab attendance and participation. If you are absent more than
twice from lab, you will lose 10 points for each additional absence, in addition to missed
quiz, assignment, and lab safety points. If you are attending but your instructor is
concerned about your participation, he/she will discuss the problem with you so you can
improve your performance.
If you are absent when a graded assignment such as a lecture quiz or exam is given,
your instructor is not required to allow you to make up the missed grades.
Lecture exams are mostly multiple choice, sometimes accompanied by a few matching
or short answer questions. The final will be comprehensive with regard to lecture
material. The course pack contains a study guided from the final.
The lab exams require you to recall, evaluate, interpret, or carry out procedures or
calculations performed in lab. There will also be questions over videos and other lab
activities. The required responses will be very short answers (no more than a few
words).
You will summarize 2 microbiology articles. The due dates are listed on the lab
schedule (due the second session of the indicated week - Wednesday or Thursday.)
The articles are assigned and will be available on library databases over the Internet
and on reserve in the library under Microbiology articles.
Incompletes are only given to those who are passing at the time of the request and who
are missing no more than one major assignment. Because lab space is limited, it is
usually not possible to take an incomplete when significant lab work must be made up.
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Anyone caught cheating on an exam or other assignment will be asked to withdraw from
the class or will receive a failing grade. Formal disciplinary action may be taken at the
instructor’s discretion.
Your instructor may provide you with additional written information about scheduling of
activities, class procedures and policies.
Please talk to or e-mail your instructor if you have any questions about completing any
assignment for the class, or if you have any concerns about your safety in the
classroom or laboratory. Your instructor wants you to have a safe and useful
experience in the class and wants to help you learn as much as you can and do as well
as possible in the class! “If you send e-mail to your instructor, please include the
word MICRO in the subject heading.” This will make it much less likely that your email gets discarded as “junk” by the college’s spam filter. Thank you!”
Student Success Center (Room C219). Services available for students include tutoring
(both scheduled one-on-one tutoring and walk-in tutoring) and Disability Support
Services. Students can drop in during the hours of operation for tutoring in the Math
Help Room (C219 and C223) or the Biology Help Room (C243A). To receive one-onone tutoring, students should make a request early in the semester in the Student
Success Center. The Student Success Center is also the place to arrange
accommodations. John A. Logan College will make reasonable accommodations for
students with documented disabilities. Contact the coordinator of Disability Support
Services, Room C219B, ext. 8516, for more information. The coordinator must
determine eligibility and arrange appropriate academic accommodations. However, it is
the student’s responsibility to register in advance of a school term with Disability
Support Services and to turn in a class schedule each term to ensure accommodations
are arranged. Free educational workshops are also provided by the Student Success
Center throughout the semester. For more information, visit the Student Success
Center website at www.jalc.edu/student_success)
English Writing Center/Tutoring. For assistance with writing assignments in any college
courses, students are encouraged to visit the Writing Center in C214. English
instructors are available for one-on-one tutoring each semester during hours posted at
the center.
Financial Aid. Students who receive financial assistance and completely withdraw from
classes prior to 60% of the semester being completed (approximately 2-3 weeks after
midterm) could be responsible to return a portion of their Federal Pell Grant award.
Prior to withdrawing from courses, students should contact the Financial Aid Office.
Course Withdrawal Information. It is expected that you will attend this class regularly.
If you stop attending for any reason, you should contact your advisor and withdraw
officially to avoid the posting of a failing grade (an E) to your transcript. It is also
advisable to discuss the situation with your instructor before dropping.
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Administrative Withdrawal. A student may be withdrawn from a class for lack of
attendance/participation as defined below. Logging into an online course is not
sufficient to be considered participation. Participation for online courses is defined as
an active process and may include posting/sending assignments to the drop box or
instructor’s email, participating in online discussion boards, taking quizzes or exams, or
otherwise communicating and/or participating in some manner that is approved by the
instructor. A student who has been administratively withdrawn from class will receive
an email notification from the Admissions Office. This notification will outline the
student’s appeal options if the student wishes to re-enroll in the course.
Face-to-Face Class
(8 or 16 weeks)
Online Class
(8 week)
Online Class
(16 week)
Student may be withdrawn from class if the student has missed
four (4) consecutive class periods without prior notification to the
instructor of the absences.
Student may be withdrawn from class if the student has not
participated (as indicated by activity reports) in the online class
for the equivalent of one week.
Student may be withdrawn from class if the student has not
participated (as indicated by activity reports) in the online class
for the equivalent of two weeks.
Safety Review Request. Our safety at JALC is everyone’s responsibility!
Faculty, staff, and students are encouraged to use the Safety Review Request,
https://secure.jalc.edu/campus_safety/safety_review_request_form.php, link on the
JALC home page or contact Campus Safety directly (Ext. 8218 or Room E105) to report
safety concerns or questions. This statement comes from the John A. Logan College
Campus Violence Prevention Committee.
METHOD OF PRESENTATION:
Lecture-discussion, small group activities, reading assignments, and supervised lab
work
TEXT:
Microbiology: An Introduction. (10th edition, 2009) Tortora, Funke and Case.
Publisher: Pearson/Benjamin Cummings. (Required) ISBN-13: 978-0-321-55007-1
Microbiology Place website subscription: recommended but not required if your book
does not come with a free subscription.
Coursepack available at College Bookstore. (Required)
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INSTRUCTOR:
Jo Forer
Office: C241
Phone Ext: 8519
Email: joforer@jalc.edu
DATE:
Spring, 2014
John A. Logan College Telephone Numbers
Carterville Campus
Carterville and Williamson County ................................. (618) 985-2828, (618) 985-3741
Carbondale and Jackson County .................................. (618) 457-7676, (618) 549-7335
Du Quoin area .......................................................................................... (618) 542-8612
West Frankfort area.................................................................................. (618) 937-3438
Crab Orchard, Gorham, and Trico Areas ................................................. 1-800-851-4720
Alongi DQ Extension Center ................................................................. (618) 542-9210
West Frankfort Extension Center........................................................... (618) 932-6639
John A. Logan College does not discriminate on the basis of race,
religion, color, national origin, disability, age, sexual orientation, or gender orientation.
11-21-13-3d SP 14
rev 01-16-14 ab
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Addendum to Syllabus for BIO 226. Policies for Jo Forer’s sections BIO 226
You will have small assignments covering lecture material almost every class period.
Some will be quizzes done on Desire 2 Learn, others will be individual or group work
done in class, and a few (study journals, article summaries) will be completed ahead of
time and brought to class. Most are worth 5 points, but sometimes 1 or 2 bonus points
are included. Your lowest 5-7 scores in this category will be dropped, to keep 150 points
possible of work. No makeups are allowed – missed scores can only be dropped. Most
semesters we drop 5-7 low lectures scores.
You will have similar small assignments in lab, including video worksheets (for which
you receive points based on attendance) and worksheets over experiments (which are
turned in and graded). Your lowest scores will be dropped here as well, to keep 150
points possible. Most semesters we drop about 5 low lab scores.
You cannot turn in worksheets for experiments you did not actually do.
If you were present when the experiment was done, but did not complete a worksheet
by the due date, I will accept worksheets up to one (1) class period late for reduced
points. In case of illness or emergency, this deadline may be extended – please notify
me by e-mail or phone as soon as possible for maximum accommodation.
As stated in the syllabus, every lab absence after the first 2 will result in loss of 10
points (1%) from your overall grade in the class. This is in addition to any points lost for
lab work you did not do. If you can make up that lab with a different section (it must be
a session that covers the material you missed in your absence) you will not lose points.
I will not deduct for absences for college-approved activities (provided you followed
correct procedures for notifying me – typically BEFOREHAND), although you do need to
make up the work you missed.
After you miss 3 classes in a row, or 5 classes total, you will not be able to attend class
again before you meet with me.
If exam dates must be changed, I will give you as much advance notice as possible. I
will try to give at least 2 weeks advance notice.
My lecture exams are 50 multiple choice questions. I try to make about 10% of the
exam application questions (5 questions) where you are asked to use information from
the class to figure out something about an unfamiliar situation that was not discussed in
class or in the readings. The other 45 questions are oriented towards recall of
definitions, concepts and processes from lecture and reading material.
Exam 5 will be a take-home exam. You will get the questions one (1) week before it is
due.
The equivalent of one lecture exam grade (“Exam 4”) comes from a one week lab
activity done near the end of the semester. It is multiple choice but is based on video
and poster presentations that occur during lab. This activity covers classification and
eukaryotic pathogens. Half the points come from the first day, the other half from the
second day. Make every effort to be there BOTH days!
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I do not give make-up lecture exams after the rest of the class has had the exam. If you
must miss a lecture exam for any reason (including approved college activities) and can
arrange to take it EARLY, I will be happy to do that. Your lowest lecture exam score will
be dropped from your grade. If you are unable to take a lecture exam for any reason
(even illness, even anything), that “0” will be dropped. I will do this automatically for
the lowest lecture exam score, whether it is a “0” or not.
College approved activities: I comply with college procedures for dealing with any
college-approved absence, which means that if you follow the correct procedures, you
will be able to make up any assignments you miss because of the activities. However,
please note that if at all possible, the college requires you to notify your instructors in
advance of any such absence and arrange to complete the missed work beforehand.
If this is not possible for a specific college-approved activity, you must notify me at the
EARLIEST POSSIBLE opportunity – use voice-mail or e-mail, don’t wait until the next
time you happen to be on campus!
Some lecture exams may be followed by a “lightning round” at my discretion. The
purpose of this activity is to encourage you to reflect upon and discuss the exam with
your fellow students, and hopefully to learn more from the exam. You can receive a
MAXIMUM of 5 points added to your score through the lightning round. More
information about this activity is available on the class Desire 2 Learn page.
Tentative Lecture Exam Schedule
Exam 1
Exam 2
Exam 3
Exam 4
Exam 5
2/11-12 (Tuesday and Wednesday)
3/19-20 (Wednesday and Thursday)
4/14-15
DONE ENTIRELY IN LAB the week of April 21 (half the exam is done
each day in lab this week)
This is a take-home exam. You will receive the questions on April 29 or 30
then the answers will be due May 6 or 7
Final: On a date to be determined when college finals schedule is published (usually
shortly after midterm).
Study aids: Your text, course pack and optional study guide contain a variety of study
aids. Please familiarize yourself with them. Mrs. Forer’s Bio 226 will have a Desire 2
Learn page, accessible from the college web site, this semester. A summary of
the study tools for the class and suggestions for using them is available from me by
request. There will be required activities on Desire 2 Learn.
Study journals: You will be asked to fill out a form called a study journal for each of
the first (3) lecture exams. The goal is to help you reflect upon your activities as a
student and, if necessary, improve your performance by changing your activities to ones
that are effective for YOU. You receive 10 points for each study journal you turn in.
This is counted as lecture points.