iNtRON Biotechnology
GENERAL USE
PRO-STAINTM Prestained Protein Marker II
[for Dual color]
Cat. No.
24061
250㎕
DESCRIPTION
PRO-STAINTM Prestained Protein Marker II is a mixture of
purified proteins covalently coupled to a blue and violet dye
that resolved to 6 bands between 19.3 and 116kDa when
electrophoresed. The product contain high-concentration of
PRO-STAINTM Prestained Protein Marker II, therefore little
amount is necessary of them. It designed for monitoring of
protein separation during SDS-PAGE, verifying western blot
transfer efficiency, perfect for CBB staining, silver staining,
and negative staining. Color remain the same even after
blotting. PRO-STAINTM Prestained Protein Marker II is supplied
in gel loading buffer and is ready-to-use.
1. Let PRO-STAINTM Prestained Protein Marker II adjust to
room temperature before use. After complet thawning, mix
them throughly .
2. For mini-gel load 5-10㎕ to the bottom of the wells by the
pipettor. For full-size gel (ex. 16 x 20 cm) load 10-20㎕.
3. Load samples.
4. Connect the power supply and start electrophoresis.
kDa
116
94
37
27.5
Store at -20℃, the quaranteed date is 2yr.
Must be aliquot the protein marker in several tube, if more
than stable.
CHARACTERISTICS
- The separation state of sample can be presumed during
eletrophoresis.
- Contain high-concentration of pre-stained protein, therefore
little amount is necessary of them.
- Very good lot-to-lot consistency.
- Perfect for CBB staining, silver staining and negative staining.
- Ready-to-use.
STORAGE BUFFER
3(W/V)% SDS
10mM DTT
2mM EDTA-2Na
0.01(W/V)% NaN3
10mM Tris-HCl (pH 6.8)
33(V/V)% Glycerol
19.3
PRO-STAINTM Prestained Protein Marker II
(for Dual color)
Apparent
PROTEIN
MW (Da)
COLOR
β-Galactosidase
Bovine serum albumin
Ovalbumin
Carbonic anhydrase
Myoglobin
Lysozyme
116,000
94,000
47,000
37,000
27,500
19,300
Blue
Violet
Blue
Violet
Blue
Violet
iNtRON Biotechnology
GENERAL USE
PRO-STAINTM Prestained Protein Marker II
[for Dual color]
Cat. No.
24061
250㎕
DESCRIPTION
PRO-STAINTM Prestained Protein Marker II is a mixture of
purified proteins covalently coupled to a blue and violet dye
that resolved to 6 bands between 19.3 and 116kDa when
electrophoresed. The product contain high-concentration of
PRO-STAINTM Prestained Protein Marker II, therefore little
amount is necessary of them. It designed for monitoring of
protein separation during SDS-PAGE, verifying western blot
transfer efficiency, perfect for CBB staining, silver staining,
and negative staining. Color remain the same even after
blotting. PRO-STAINTM Prestained Protein Marker II is supplied
in gel loading buffer and is ready-to-use.
1. Let PRO-STAINTM Prestained Protein Marker II adjust to
room temperature before use. After complet thawning, mix
them throughly .
2. For mini-gel load 5-10㎕ to the bottom of the wells by the
pipettor. For full-size gel (ex. 16 x 20 cm) load 10-20㎕.
3. Load samples.
4. Connect the power supply and start electrophoresis.
kDa
116
94
- The separation state of sample can be presumed during
eletrophoresis.
- Contain high-concentration of pre-stained protein, therefore
little amount is necessary of them.
- Very good lot-to-lot consistency.
- Perfect for CBB staining, silver staining and negative staining.
- Ready-to-use.
•
•
•
•
•
•
3(W/V)% SDS
10mM DTT
2mM EDTA-2Na
0.01(W/V)% NaN3
10mM Tris-HCl (pH 6.8)
33(V/V)% Glycerol
Apply 5㎕ on mini-gel and electrophoresed for 1hr at 15% SDS-PAGE
gel. After electrophoresed, transfer for
2hr at 80 volt.
37
27.5
Store at -20℃, the quaranteed date is 2yr.
Must be aliquot the protein marker in several tube, if more
than stable.
CHARACTERISTICS
8-20% SDS-PAGE Blot
47
STORAGE
STORAGE BUFFER
Apply 5㎕ on mini-gel and electrophoresed for 1hr at 15% SDS-PAGE
gel. After electrophoresed, transfer for
2hr at 80 volt.
47
STORAGE
•
•
•
•
•
•
8-20% SDS-PAGE Blot
19.3
PRO-STAINTM Prestained Protein Marker II
(for Dual color)
Apparent
PROTEIN
MW (Da)
COLOR
β-Galactosidase
Bovine serum albumin
Ovalbumin
Carbonic anhydrase
Myoglobin
Lysozyme
116,000
94,000
47,000
37,000
27,500
19,300
Blue
Violet
Blue
Violet
Blue
Violet