BISC300 --- INTRODUCTION TO MICROBIOLOGY
Spring 2011---LABORATORY SYLLABUS
• Eye protection is required in the laboratory. Purchase splash goggles or safety
glasses prior to the first lab.
• Bring marking pens to each lab meeting.
• Read lab exercises before coming to lab.
• Skills and techniques learned in this course are cumulative. It is expected that
you will master and use them repetitively.
• Use this syllabus each week to study and plan your work prior to lab. Written
work flow charts will be required starting Lab 2. A sample flow chart is
provided here as an example covering a lab with multiple exercises on a single
day.
• General information on the lab, including safety regulations and grading
procedure is contained at the front of the lab manual. Read very carefully.
• Students occasionally have legitimate reasons to miss lab on a given week,
usually because of illness. Since all sections are filled this semester, it is
essential that students who must miss a lab follow the procedures for
scheduling a makeup lab. Permission to take a makeup lab may be denied if the
reason is not an excused absence, as defined by the University.
LAB DATE
2/7
2/14
CONTENT
NO LABS these weeks
•
Purchase lab manual & goggles (Bookstore)
Bring these to Lab 1.
Reading assignment: Lab Manual Introduction /Safety + Ex. 1
1
2/21
SAFETY LECTURE
Ex. 1 Brightfield Microscopy
Purchase slides and latex-free gloves this week as you will need them
next week in lab. Meet a lab partner this week with whom you can
share these purchases as you will not need whole boxes individually.
They are available in Room 009 McKinly Lab, also known as The
Fisher Stockroom. Credit or debit cards are accepted..NO cash or
checks. The Stockroom is open 10am-noon and 1pm -4pm Monday
through Friday.
2
2/28
ASEPTIC TECHNIQUES LECTURE
Ex. 2 Microbiological Laboratory Techniques
Bring slides and Sharpie marking pens.
3
3/7
DISPLAY 1: BACTERIA WITH SPECIAL PROPERTIES
Ex 2. Lab Techniques, Week II
Ex. 3 The Gram Stain
4
3/14
Ex. 5 Pure Culture Techniques, Week I
DISPLAY 2: OXYGEN REQUIREMENTS
Ex. 2 Lab Techniques, Week III
Ex. 5 Pure Cultures, Week II
5
3/21
Dilution Worksheet…. ( beginning work for EX 4)
DISPLAY 4: DIFFERENTIATING BACILLUS SPECIES
Ex. 8 Staphylococci and Streptococci, Week I
Ex. 4 Bacterial Growth Curve- Standard Curve
Ex. 5 Pure Cultures, Week III…. Lab Report due: Ex 5
3/28
6
4/4
NO LABS – SPRING BREAK
Ex. 8 Staph and Strep, Week II
Ex 4 Bacterial Growth Curves continue
Ex. 7 Antibiotic Sensitivity Testing, Week I
Ex. 9 Gram Negative Enteric Pathogens,Week I
N.B. Students must come in 24 hours after lab time to record the results of
TSIA slants. Bring your lab manual to record your observations on the
proper report pages. Cell phone cameras are not an acceptable form of
recording your results.
7
4/11
Ex. 8 Staph and Strep, Week III…Ex 8 lab report due today
Ex. 7 Antibiotic Sensitivity Testing,Week II
Ex. 9 Gram Negative Enteric Pathogens, Week II
Lab Report due: Ex 4 The Growth Curve
8
4/18
Ex. 9 Gram Negative Enteric Pathogens,Week III
REVIEW OF MICROORGANISMS-Receive handout in lab
Ex. 11 Identification of Unknowns, Week I
N.B. Students must come in 24 hours
after lab time to record the results of
TSIA slants
9
4/25
10
5/2
Lab report due: Ex 7
Ex. 11 Identification of Unknowns, Week II
Lab Report Due today : Ex. 11 Unknowns
Ex. 10 Microbiology of Food, Week I
Ex 10 Microbiology of Food, Week II. Lab report Ex 10 due
Review Session for lab Practical and Exam
11
5/9
LAB PRACTICAL & LAB FINAL EXAM
Bring Sharpie marking pens, slides, gloves.