DNA and RNA Purification
Selection Guide
Spend less time isolating DNA or RNA and more time doing experiments and
interpreting results! Whatever your starting material or your application we
have the solution…
Choosing a suitable DNA or RNA isolation system for your downstream application
is vital for the success of the experiment. For ease-of-use, Promega offers an array
of conveniently packaged purification products to isolate plasmid, genomic and
fragment/PCR products quickly, using safe methods via manual or
automated procedures.
DNA Purification
≤200μl
Sample volume
Technology
Time to Process
(or amount)
Yield
Format
(*V=variable)
(Auto/Manual)
100 preps
£194
A5082
250 preps
£455
A1120
100 isolations x 300μl
£166
50μl to 10ml
Wizard® Genomic DNA Purification Kit
Simple, solution-based isolation of DNA from white blood cells, tissue culture
cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative
bacteria. Reagent volumes can be scaled to correspond to the amount of
material processed.
A1125
500 isolations x 300μl
£328
A1620
100 isolations x 10ml
£706
A1751
96 x 10ml to 960 x 1ml preps
£860
3 - 10ml
ReliaPrep™ HT gDNA Isolation System
Scalable isolation from 3–10ml of blood. No centrifugation or hazardous
chemicals. Can be automated on Tecan Freedom Evo Workstation for walkaway
purification, regardless of sample storage or shipping conditions. For
low-throughput isolation of up to 32 samples, the ReliaPrep™ LV 32
Heater Shaker Magnet can be used in a manual mode.
1cm (mouse tail)
or up to 25mg
one buccal swab
ReliaPrep™ gDNA Tissue Miniprep System
Isolate pure, intact gDNA without alcohol washes or precipitations from
up to 25mg of tissue, a buccal (cheek) swab, or a 1cm mouse tail snip.
Ready-to-use reagents. Good recovery and purity in as little as 50μl and
with better A260/A230 ratios than competitors.
A2051
100 preps
£207
A2052
250 preps
£461
11mg (mouse liver)
0.5 -1cm (mouse tail)
40mg (tomato leaf)
Wizard® Genomic DNA Purification Kit
Simple, solution-based isolation of DNA from white blood cells, tissue culture
cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative
bacteria. Reagent volumes can be scaled to correspond to the amount of
material processed.
A1120
100 isolations x 300μl
£166
A1125
500 isolations x 300μl
£328
A1620
100 isolations x 10ml
£706
1.2cm (mouse tail)
or up to 20mg
Wizard® SV Genomic DNA Purification System
Fast, simple, membrane-based technique delivers high yields of pure DNA.
Use either a microcentrifuge (spin) or vacuum protocol.
A2360
50 preps
£80
A2361
250 preps
£332
BLOOD
A5081
≤200μl
ReliaPrep™ Blood gDNA Miniprep System
Isolate pure, intact gDNA without alcohol washes or precipitations from fresh or
frozen blood, with ready-to-go reagents. Good recovery and purity in as little as
50μl and with better A260/A230 ratios than competitors.
TISSUE
TISSUE
BLOOD
Applications include: in vitro transcription, cloning, probe generation, sequencing, PCR, restriction digest and transformation.
1 x 10 to 8 x 10
culture cells
CELLS
6
1 x 104 to 5 x 106
culture cells
600μl to 3ml
bacterial culture
25 to 100ml
PLASMIDS
6
Up to 250ml
Technology
(or amount)
Time to Process
Yield
Format
(*V=variable)
(Auto/Manual)
ReliaPrep™ FFPE gDNA Miniprep System
Complete, all-inclusive method for formalin-fixed, paraffin-embedded tissue
without using hazardous solvents or overnight digestion and with minimal
hands-on time. Optimised lysis and binding conditions reverse modifications
introduced by the fixation process, resulting in intact, amplifiable DNA.
A2351
10 preps
£52
A2352
100 preps
£309
Wizard® Genomic DNA Purification Kit
Simple, solution-based isolation of DNA from white blood cells, tissue culture
cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative
bacteria. Reagent volumes can be scaled to correspond to the amount of
material processed.
A1120
100 isolations x 300μl
£166
A1125
500 isolations x 300μl
£328
A1620
100 isolations x 10ml
£706
A2360
50 preps
£80
A2361
250 preps
£332
A1223
100 preps
£123
A1222
200 preps
£279
A2492
25 preps
£139
A2495
100 preps
£492
A2496
300 preps
£1364
A2392
10 preps
£121
A2393
25 preps
£283
Wizard® SV Genomic DNA Purification System
Fast, simple, membrane-based technique delivers high yields of pure DNA.
Use either a microcentrifuge (spin) or vacuum protocol.
Miniprep
Midiprep
Maxiprep
PureYield™ Plasmid Systems
Rapidly isolate transfection-quality plasmid DNA. Vacuum protocol
greatly reduces the time spent on purification compared to silica
resin or other membrane-column methods. Unique Endotoxin
Removal Wash removes substantial amounts of protein, RNA and
endotoxin contaminants from purified plasmid DNA to improve the
robustness of sensitive applications such as eukaryotic transfection,
in vitro transcription and coupled in vitro transcription/translation.
No isopropanol precipitation of purified plasmid DNA or extensive
centrifugation, providing rapid purification from a single method.
FFPE
Sample volume
CELLS
5 - 50μm tissue
sections
≤200μl
PLASMIDS
FFPE
DNA Purification
1 - 10ml culture
Wizard® Plus SV Miniprep DNA Purification System
Silica membrane-based. Purified plasmid DNA can be used directly for automated
fluorescent BigDye® terminator DNA sequencing and standard molecular biology
techniques without further manipulation. No phenol extractions or precipitations.
Use either a microcentrifuge (spin) or vacuum protocol.
40D600 culture
Wizard® SV 96 Plasmid DNA Purification System
Silica-membrane, 96-well, high-throughput format. Purified plasmid can be used
directly for automated, fluorescent DNA sequencing and standard molecular
biology techniques, including restriction enzyme digestion. Either manual or
automated on Beckman Coulter or PerkinElmer instruments.
60D600 culture
Wizard® MagneSil® Plasmid DNA Purification System
96-well, high-throughput format. Purified plasmid can be used directly for
automated fluorescent sequencing, like BigDye® terminator sequencing
chemistry, and standard molecular biology techniques. MagneSil® Paramagnetic
Particles for lysate clearing as well as DNA capture circumvent the need for
centrifugation or vacuum manifolds, making the system ideal for full automation
on many different automated instruments.
≤200μl
Sample volume
Technology
(or amount)
Time to Process
Yield
Format
(*V=variable)
(Auto/Manual)
A1330
50 preps
£64
A1460
250 preps
£263
A1465
1000 preps
£942
A2250
1 x 96 preps
£200
A2255
5 x 96 preps
£801
A2258
100 x 96 preps
£7308
A1630
4 x 96 preps
£344
A1631
8 x 96 preps
£649
A1635
100 x 96 preps
£6688
PLASMIDS
PLASMIDS
DNA Purification
Up to 200μl PCR
reaction
Wizard® SV 96 PCR Clean-Up System
High-throughput purification of 100bp to 10kb PCR products from excess
nucleotides, primers and primer dimers with recovery of >90%. Purified
DNA can be used for automated fluorescent sequencing, cloning, labelling,
restriction digestion or microarray analysis without further manipulation.
Protocols are available for automated instruments from Beckman Coulter,
Eppendorf, PerkinElmer and Tecan.
20μl sequencing
reaction
20mg tissue
Wizard® MagneSil® Sequencing Reaction Clean-Up System
High-throughput purification of sequencing reactions, including BigDye®
Terminator reactions, performed using MagneSil® GREEN Paramagnetic
Particles with standard, nonskirted 96-well amplification plates. No user
intervention required until the samples are ready to load onto sequencer.
Protocols are available for automated instruments from Beckman
Coulter, Eppendorf, PerkinElmer and Tecan.
Wizard® SV Genomic DNA Purification System
Fast, simple, membrane-based technique delivers high yields of pure DNA.
Use either a microcentrifuge (spin) or vacuum protocol.
Sample volume
Technology
(or amount)
Time to Process
Recovery
Format
(Auto/Manual)
A9281
50 preps
£67
A9282
250 preps
£299
A9285
1000 preps
£1071
A9340
1 x 96 preps
£119
A9341
4 x 96 preps
£430
A9342
8 x 96 preps
£740
A9345
100 x 96 preps
£8515
A1831
4 x 96 preps
£284
A1832
8 x 96 preps
£486
A1835
100 x 96 preps
£3567
A2360
50 preps
£80
A2361
250 preps
£332
FRAGMENTS
Up to 350mg agarose
gel slice or up to 1ml
enzymatic reaction
Wizard® SV Gel and PCR Clean-Up System
Extracts and purifies DNA fragments of 100bp to 10kb from standard
or low-melting agarose gels or purifies products directly from PCR
or restriction digests with up to 95% recovery. Binds up to 40μg
of DNA which can then be used for automated fluorescent DNA
sequencing, cloning, labelling, restriction enzyme digestion or
in vitro transcription/translation with further manipulation.
≤200μl
PLANT
PLANT
FRAGMENTS
DNA Purification
RNA Purification
≤200μl
Sample volume
Technology
(or amount)
Time to Process
Yield
Format
(*V=variable)
(Auto/Manual)
BLOOD
100μl blood
Up to 20μl blood
SV Total RNA Isolation System
Fast and simple preparation of intact total RNA from tissue, cultured cells and
white blood cells. DNase treatment on the minicolumn membrane substantially
reduces genomic DNA contamination, which can interfere with
amplification-based methodologies. No phenol:chloroform extractions
or ethanol precipitations, and no DNase carryover in the final RNA
preparation. Use either a microcentrifuge (spin) or vacuum protocol.
MagneSil® Total RNA Mini-Isolation System
High-throughput 96/384-well format for fast, simple preparation of intact
total RNA from tissue culture cells, tissue lysate or freshly isolated whole
blood. The protocol enables high-throughput automated purification on a
variety of liquid-handling workstations. No vacuum filtration, centrifugation
or precipitation. Elution volumes as low as 15μl provide concentrated RNA for
endpoint RT-PCR amplification and real-time RT-PCR for example.
Z3101
10 preps
£58
Z3100
50 preps
£198
Z3105
250 preps
£777
Z3351
4 plate
£883
BLOOD
Applications: gene expression analysis, microarrays, RNA-SEQ, RT-PCR and qRT-PCR.
≤200μl
Sample volume
Technology
(or amount)
Time to Process
Yield
(*V=variable)
Format
(Auto/Manual)
10 preps
£58
Z3100
50 preps
£198
Z3105
250 preps
£777
Z3500
1 x 96 each
£276
150 - 300mg
SV 96 Total RNA Isolation System
High throughput technique with novel vacuum manifold to eliminate
waste handling and novel plate design to prevent cross-contamination.
DNase treatment step substantially reduces gDNA contamination.
No phenol:chloroform extraction or ethanol precipitation.
Z3505
5 x 96 each
£1216
Z3351
4 plate
£883
≤2mg tissue lysate
in 100μl
MagneSil® Total RNA Mini-Isolation System
High-throughput 96/384-well format for fast, simple preparation of intact
total RNA from tissue culture cells, tissue lysate or freshly isolated whole
blood. The protocol enables high-throughput automated purification on a
variety of liquid-handling workstations. No vacuum filtration, centrifugation
or precipitation. Elution volumes as low as 15μl provide concentrated RNA
for endpoint RT-PCR amplification and real-time RT-PCR for example.
Z1001
10 reactions
£62
5 -50μm tissue
sections
ReliaPrep™ FFPE Total RNA Miniprep System
All-inclusive method for quality total RNA from formalin-fixed
paraffin-embedded tissue without using hazardous solvents or overnight
digestion. Total RNA can be isolated from FFPE tissue quickly and with
minimal hands-on time, resulting in high quality, intact, amplifiable RNA.
Z1002
100 reactions
£464
TISSUE
Z3101
15 - 60mg
SV Total RNA Isolation System
Fast and simple preparation of intact total RNA from tissue, cultured cells and
white blood cells. DNase treatment on the minicolumn membrane substantially
reduces genomic DNA contamination, which can interfere with
amplification-based methodologies. No phenol:chloroform extractions
or ethanol precipitations, and no DNase carryover in the final RNA
preparation. Use either a microcentrifuge (spin) or vacuum protocol.
FFPE
FFPE
TISSUE
RNA Purification
RNA Purification
PLANT
≤5 x 106 tissue
culture cells
SV 96 Total RNA Isolation System
High throughput technique with novel vacuum manifold to eliminate
waste handling and novel plate design to prevent cross-contamination.
DNase treatment step substantially reduces gDNA contamination.
No phenol:chloroform extraction or ethanol precipitation.
≤1 x 10 tissue
culture cells
MagneSil® Total RNA Mini-Isolation System
High-throughput 96/384-well format for fast, simple preparation of intact
total RNA from tissue culture cells, tissue lysate or freshly isolated whole
blood. The protocol enables high-throughput automated purification on a
variety of liquid-handling workstations. No vacuum filtration, centrifugation
or precipitation. Elution volumes as low as 15μl provide concentrated RNA
for endpoint RT-PCR amplification and real-time RT-PCR for example.
Leaf tissue up
to 300mg
PureYield™ RNA Midiprep System
For intact, pure total RNA from essentially any sample and for use in a wide range
of applications such as RT-PCR. Novel Clearing Agent enables rapid purification
with undetectable levels of genomic DNA contamination without using DNase.
No organic solvents, protease digestions or alcohol precipitations. Designed for
use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum
Manifold) formats.
5
Technology
(or amount)
Time to Process
Yield
Format
(*V=variable)
(Auto/Manual)
Z6010
10 preps
£50
Z6011
50 preps
£158
Z6012
250 preps
£725
Z3101
10 preps
£58
Z3100
50 preps
£198
Z3105
250 preps
£777
Z3500
1 x 96 each
£276
Z3505
5 x 96 each
£1216
Z3351
4 plate
£883
Z3740
10 preps
£90
Z3741
50 preps
£375
CELLS
CELLS
≤5 x 106 tissue
culture cells
Sample volume
PLANT
100 to 5 x 106
tissue culture cells
ReliaPrep™ RNA Cell Miniprep System
Proprietary column/binding matrix efficiently captures RNA from very
small amounts of input material, isolating RNA eluted in a minimal
volume (less than 15μl). DNase treatment step directly on the
minicolumn membrane effectively removes substances that
inhibit downstream assays. No phenol:chloroform extractions
or ethanol precipitations. Delivers ready-to-use pure total RNA that
does not require concentration in demanding applications.
SV Total RNA Isolation System
Fast and simple preparation of intact total RNA from tissue, cultured cells
and white blood cells. DNase treatment on the minicolumn membrane
substantially reduces genomic DNA contamination, which can interfere
with amplification-based methodologies. No phenol:chloroform extractions
or ethanol precipitations, and no DNase carryover in the final RNA
preparation. Use either a microcentrifuge (spin) or vacuum protocol.
≤200μl
Maxwell® 16 System
Process more samples in less time. Reliable, rapid, automated
nucleic acid purification for any laboratory!
Automated extraction in a compact, benchtop system that easily
fits into a biosafety cabinet. Maxwell uses paramagnetic-particle
based technology for faster separations.
•
•
•
•
•
•
•
Process 1-16 samples simultaneously
Fast protocols ≈ 45minutes
Consistent high yields
No cross-contamination
Homogenisation for tissue samples
Free downloadable methods for DNA, RNA and protein extractions
Flexible configurations of standard or low elution volumes (SEV/LEV)
Extensive range of optimised, pre-filled extraction kits for all sample types:
tissue, blood, FFPE, viral, cultured cells, buccal swabs and other biological fluids and samples.
www.promega.com/maxwell
Promega UK Ltd
Delta House
Southampton Science Park
Southampton
SO16 7NS
www.promega.com