LONG-TERM MONITORING OF TAMMAR (Macropus eugenii derbianus) AND BLACK-GLOVED WALLABIES (Macropus irma) IN THE FITZ-STIRLING OPERATIONAL AREA OF GONDWANA LINK MONITORING PROTOCOLS AND MONITORING SITE DESIGN FINAL REPORT February 2010 Tammar captured on remote camera (right; with joey in pouch) Black-gloved wallaby captured on remote camera (right; with joey in pouch) 1 Contents List of Figures and Tables .................................................................................................................. 3 ACKNOWLEDGEMENTS ............................................................................................................................. 3 1. INTRODUCTION ..................................................................................................................................... 4 2. REAPPRAISAL OF THE SPRING 2008 MONITORING SEASON................................................................. 4 a) Detection by scats and remote cameras .......................................................................................... 5 b) Detection by remote hair collection ................................................................................................. 5 c) Distance animal observed from site.................................................................................................. 6 d) Species recorded previously or subsequently at the site within a year of a monitoring session. ... 6 Revised results for Spring 2008 Monitoring Season ................................................................................. 7 Faecal DNA as an alternative detection method .............................................................................. 7 3. TARGETED SEARCH FOR TAMMAR POPULATIONS ............................................................................... 8 4. MONITORING PROTOCOLS ................................................................................................................. 10 Detection methods ................................................................................................................................. 10 1. Signs ................................................................................................................................................ 10 Scats ................................................................................................................................................ 10 Footprints ........................................................................................................................................ 12 Remote hair collection .................................................................................................................... 15 2. Direct observation........................................................................................................................... 17 Spotlighting ..................................................................................................................................... 17 Remote sensing cameras ................................................................................................................ 18 Detection rates for direct observation methods (spotlighting and remote cameras) ....................... 20 5. SUMMARY OF RESULTS – MARCH 2007 to DECEMBER 2009. ............................................................ 22 6. MONITORTING PROTOCOLS FOR TAMMARS AND BLACK-GLOVED WALLABIES ................................ 23 a) Black-gloved wallabies............................................................................................................... 23 i. Detection methods .................................................................................................................... 23 ii. Monitoring site design and review of FLP target and objective ................................................ 24 iii. Recommendations..................................................................................................................... 24 b) Tammars ............................................................................................................................................. 27 i. Detection methods .................................................................................................................... 27 ii. Monitoring site design and review of FLP target and objective ................................................ 27 iii. Recommendations..................................................................................................................... 28 Options for monitoring site design. ................................................................................................ 28 Possible alternatives for FLP objective ........................................................................................... 29 7. APPENDICES ........................................................................................................................................ 30 Appendix A: Spring 2008 monitoring site codes and GPS coordinates............................................... 30 Appendix B: Spatial datasets relating to the project .......................................................................... 33 Appendix C: TripWatch recording sheet ............................................................................................. 35 Appendix D: Wallaby occurrence and survey effort on GLink Properties .......................................... 38 8. REFERENCES ........................................................................................................................................ 43 2 List of Figures and Tables Table 1: Detection and non-detection of suspected wallaby scats at the permanent monitoring sites...... 7 Figure 1: All known records for Tammars and Black-gloved wallabies throughout the Fitz-Stirling Operational Area.............................................................................................................................. 9 Figure 2: Comparison of the size and morphology of scats of the three Macropod species present in the area. ............................................................................................................................................... 11 Figure 3: Comparisons between the size and morphology of the foot (short pes) and footprints of the three Macropod species present in the area................................................................................. 14 Figure 4: A Tammar inspects a hair arch at the Nowanup NE corner site, captured on remote camera. . 15 Figure 5: A Tammar about to move under a fence at the Nowanup NE corner site, captured on remote camera. .......................................................................................................................................... 16 Figure 6: A horizontal sticky pole fixed between two moort trees at the Corackerup NR southern boundary Tammar site. .................................................................................................................. 16 Table 3: Remote camera results for sites where Tammars were ultimately detected. Sites in bold required more than one monitoring session before an animal was detected. ............................. 21 Table 4: Summary of the number of Tammar and Black-gloved wallaby sightings over the three years of the project...................................................................................................................................... 22 Figure 7: Locations of permanent monitoring sites and their occupancy status in Spring 2008. .............. 26 ACKNOWLEDGEMENTS This project could not have been possible without the generous assistance of many volunteers and the cooperation and interest of a number of landholders in the area. So thank you to all those that helped on field trips and to those that gave up valuable time to assist me on their properties. The project was funded by Greening Australia and Bush Heritage Australia. A special thank you to Angela Sanders (Bush Heritage) and Paul Deegan (formerly Greening Australia) and Amanda Keesing from the Gondwana Link Office for valuable support throughout the project. 3 1. INTRODUCTION The Wallaby Project commenced in March 2007 with the objective of monitoring long-term changes in abundance and distribution of Tammar (Macropus eugenii derbianus) and Black-gloved (Macropus irma) wallabies within the Fitz-Stirling Operational Area of Gondwana Link. The long-term aim of the project was to assess the impact on wallaby populations of an integrated regional scale fox baiting program and provision of more habitat through restoration and increased linkages. In the Functional Landscape Plan (FLP) for the Fitz-Stirling Operation Area (FLP 2008) the objective relating to the wallabies target is to: By 2017, increase the populations of Tammars and Black-gloved Wallabies within the Fitz-Stirling area by 30%. This objective was provisional on establishing current / baseline population levels with a higher degree of confidence than was currently available. It is planned to be reviewed by 2010. Preliminary investigations in 2007 recommended that the attribute most suitable to measure this target was the level of occupancy of each species (calculated by the proportion of sites occupied across the landscape (Gilfillan 2008a-Monitoring Site Design). Permanent monitoring sites were set up during 2008 and the first monitoring session occurred in Spring 2008. A Report was produced outlining the Spring 2008 monitoring results (Gilfillan 2008b – Spring 2008 Monitoring Results). This Final Report outlines the following: A reappraisal of the Spring 2008 monitoring results based on further investigations in 2009. Results of a targeted search for Tammars during 2009. Summary of results for the three year period. Monitoring protocols and recommendations for monitoring site design for both species. Information on spatial datasets. A review of the FLP objective and target for these species. 2. REAPPRAISAL OF THE SPRING 2008 MONITORING SEASON The first season of monitoring in Spring 2008 (outlined in detail in Gilfillan 2008), was successful in establishing a baseline level of occupancy for Black-gloved wallabies of 0.3666 (+/-0.0754) (PRESENCE program, McKenzie et al. 2002). The detection methods employed were successful for this species, 4 being detected at 6 out of the 18 sites surveyed: two sites via remote camera and four sites via spotlighting. Animals were not detected on any site via both methods. The level of occupancy for Tammars in the first monitoring season was zero; it was not detected on any site via either spotlighting or remote cameras. However, this is not believed to be a robust estimate of Tammar abundance due the apparently very patchy nature of Tammar populations across the landscape in relation to the density and number of monitoring sites, and possibly to the inefficiency in detecting this species by the survey techniques employed. Possible Brush wallaby or Tammar scats were detected at 12 of the 18 sites (Table 1). However, at the time of survey the presence of scats was not used to indicate presence of wallaby species due to the inaccuracy of determining species through scat morphology. With additional data on wallaby occurrence collected over the subsequent year and refinement of monitoring techniques (increased expertise in distinguishing scats) the conclusions reached in Spring 2008 should be reappraised as follows. a) Detection by scats and remote cameras Increased confidence and expertise in distinguishing wallaby scats based on morphology was developed during 2009. Distinctions between Western Grey Kangaroo and “wallaby” scats could be made with a large degree of confidence and some possible distinction could be made between the two wallaby species, although with less confidence. Therefore during Spring 2009 all the monitoring sites were searched again for potential wallaby scats (during a revisit to install permanent markers). Suspected wallaby scats were found at 10 out of 18 sites (9 of these were the same sites as Spring 2008 and one additional site where they were not detected in Spring 2008) (Table 1). In the light of this discrepancy in detection between animal signs (scats ) and actual sightings (remote camera or spotlighting), three of the sites where wallabies were not detected by these techniques but scats were found in both Spring 2008 and 2009 were re-sampled in Spring 2009 using remote cameras. However, four remote cameras were used at one camera site instead of only one camera, as was the case in Spring 2008, to assess whether additional cameras would increase the probability of detection of the species. In addition, a further three non-monitoring sites where suspected scats were detected were surveyed with four cameras. Despite scats being present, no wallabies were detected with four cameras at any of the sites. b) Detection by remote hair collection An additional detection technique of remote hair collection was trialed in 2009. These trials were conducted at sites where Tammars or Black-gloved wallabies were known to occur (previously detected by spotlighting or remote cameras).) Remote hair collection was conducted at two monitoring sites where wallaby scats were detected in Spring 2008 or Spring 2009 (WA02 and WA06). However, the technique provided limited success in detecting Tammars and no success in detecting Black-gloved wallabies where they were known to occur (see below), so nothing can be concluded about occupancy at these two sites based on this technique. 5 c) Distance animal observed from site In the Spring 2008 Monitoring Season a site was considered to be occupied by a species if an animal was detected “within 1 km of the point location of the site”. This distance was based on the upper limits of home range estimates of the two wallaby species determined from data in other parts of the species range (c. 50 ha, Christensen (1980); Bamford and Bamford (2002)), and a reasonable assessment of the distance animals would move in a night, based on known aspects of species ecology. However a less rigid and more reasonable assessment of occupancy might be based on whether there is similar continuous habitat between where the animal was observed and the site location, with no obstruction to movement such as large creeks or large areas of pasture between them. For example; the NE corner of Nowanup is occupied by Tammars. WA17 is 1.7 km from this site. No tammars were detected at WA17 within the period of monitoring or subsequently, however there is continuous similar habitat with no barriers to movement between the two sites and it is therefore possible that it could be occupied as part of expansion (seasonal?) of the subpopulation at NE corner site. at WA18 a Black-gloved wallaby was detected 200 m away during the monitoring session and the site was therefore classed as occupied. However the animal was observed in a different habitat type to that of the site and at a much higher position in the landscape. No scats which appeared to be Black-gloved wallaby were found on site. at WA17 a Black-gloved wallaby was detected 620 m away from the site during the monitoring session and the site was therefore classed as occupied. However the animal was observed in a different habitat type to that of the site and no wallaby scats were found at the site. d) Species recorded previously or subsequently at the site within a year of a monitoring session. At two sites where wallabies were not detected during a monitoring session they were detected by direct methods at the site, or close to, within a year of the monitoring session. Also these sites had likely indirect evidence of the species presence at the time of monitoring. WA01- Tammar subsequently detected 700m away in September 2009. In addition, likely Tammar scats and footprints were found in 2008 and 2009. WA18 - Tammar detected 600m away from the site previously in July 2007. In addition, likely Tammar scats and footprints were found in 2008 and 2009. WA06 – a Black-gloved wallaby was observed on the monitoring site by the landholders (Eddy and Donna Wajon) in October 2008. Possible scats were also detected in Spring 2008. 6 WA14 – a Black-gloved wallaby was observed by the landholder (Fred Powell), approximately 300m away in 2007/8, likely scats were detected in 2009. Revised results for Spring 2008 Monitoring Season In the light of the foregoing discussion it is “possible that” 12 sites could be considered to be occupied by Black-gloved wallabies or Tammars in Spring 2008 (if scats are used to indicate suspected presence), plus two sites by Tammars, and two by Black-gloved wallabies, based on subsequent or previous detection (Table 1). This is an alternative scenario to original conclusions made about occupancy (6 sites by Black-gloved wallabies and none by Tammars). Although these sites cannot be considered occupied in terms of calculating % occupancy, the non detection within the monitoring session likely reflects the limitations of the detection methods and therefore these sites should be considered occupied when devising management actions, or for detecting future changes in occupancy. Table 1: Detection and non-detection of suspected wallaby scats at the permanent monitoring sites. Monitoring Site WA01 WA02 WA03 WA04 WA05 WA06 WA07 WA08 WA10 WA11 WA13 WA14 WA15 WA16 WA17 WA18 WA19 WA20 Wallaby scats detectedSpring 2008 y y y y y y n y y y n n n n n y y y Wallaby scats detectedSpring 2009 y y y n y n n n y y n y n n n y y y Actual sighting (Spring 2008) Tammar BG wallaby Possible alternative occupancy scenario Tammar BG Either wallaby species n n n n n n n n n n n n n n n n n n y n n n n n n n n n n n n n n y n n y n n n n n n y y n n n n n y y n y y n n n n y n y y n n y n n n n n y y y y y y y n y y y n n n n n y y y Faecal DNA as an alternative detection method The above anomalies between detection via spotlighting or cameras and possible occupancy based on 7 presence of scats are a strong argument for the use of faecal DNA to distinguish species and hence determine presence. The use of faecal DNA has been suggested as a non-invasive technique to track individual animals to gain an understanding of the demograhic, behavioural and life-history strategies of individuals and populations (Piggott and Taylor 1993, Piggott et al. 2006, Berry et. al 2007). This technique has been successfully trialled in the identification of the presence of sympatric Quokka, Tammar Wallaby (WA), Brush Wallaby and Western Grey Kangaroo (Alacs et al. 2003). 3. TARGETED SEARCH FOR TAMMAR POPULATIONS The level of occupancy for Tammars in the first monitoring season was zero; it was not detected on any site via either survey method. However, this is not believed to be a robust estimate of Tammar abundance due the apparently very patchy nature of Tammar populations across the landscape in relation to the density and number of monitoring sites, and possibly to the inefficiency in detecting this species by the survey techniques employed The two species appear to be distributed throughout the landscape quite differently. The Black-gloved wallaby is quite widespread, occurring in a number of different vegetation types, and observed to be largely solitary. In contrast, the Tammar, based on limited observations within the Fitz-Stirling, occurs in small possibly isolated patches, and are likely to be more habitat specific. The type of habitat they occur in may also be quite uncommon in the landscape (results of habitat model, being developed by Ayesha Tulloch of University of Queensland, will support or refute this). They are also known in the forest region to be group territorial, with small patches of habitat occupied by groups of 30-40 individuals (Christensen 1980). Again, based on limited observations, this also appears to be the case within the Fitz-Stirling, although groups appear to be smaller. A targeted search for Tammars was therefore the priority for 2009 in an attempt to establish a baseline distribution of this species across the landscape. The detection techniques used during this targeted search were similar to those used in the Spring 2008 monitoring season, with some modifications and trialing of new techniques (see below). Survey for the presence of tammars was concentrated in areas of past records, where landholders had reported sightings in the past, or where suspected tammar scats had been detected. Fourteen sites were surveyed during this time. All sites used remote cameras as the detection method. In addition spotlighting was carried out 6 sites, and remote hair collection at 10. Tammars were detected at five of these fourteen sites (including the BHA property Chereninup), increasing the total number of sites known to be currently occupied by tammars in the Fitz-Stirling to 12. Presence of tammars at all of the five sites was confirmed by remote cameras. Presence at two sites was confirmed by remote hair collection and at one site by spotlighting also (Figure 1). 8 Figure 1: All known records for Tammars and Black-gloved wallabies throughout the Fitz-Stirling Operational Area. Grouped into records from 1996-2000 and 2001 – 2010. 9 4. MONITORING PROTOCOLS Detection methods Non-invasive detection methods can either be indirect (observations of signs left by animals) or direct (observations of animals themselves). During this study both methods were employed to detect the presence of animals at a site. 1. Signs Only one other macropod species is known to occur in the area, the Western Grey Kangaroo (Macropus fuliginosus). This species is much larger than the wallaby species. Woylies (Bettongia penicillata) are known historically form the area but no current records exist for this species. It is possible that they still do occur, however if so they do so they would likely be in low densities, therefore when making distinctions of signs only the Western Grey Kangaroo is considered. Scats Distinctions between Western Grey Kangaroo and “wallaby” scats based on morphology can be made with a large degree of confidence (although some Western Grey Kangaroo scats can look similar to wallaby scats) (Figure 2). Definitive distinction between the two wallaby species scats based on morphology is not possible (Figure 2). This has also been found in other studies (Alacs et al. (2003) found that the scats of these two species were hard to distinguish based on size and morphology). However, combined with other information (eg. known presence, habitat type), some conclusions can be made about which species the scat is likely to represent, therefore this technique has some use in determining presence. Scats can therefore be used to: o provide reasonable confirmation that animals are still present at a known occupied site o provide an initial quick assessment of possible occupancy by a wallaby species o target areas for more definitive survey techniques (spot lighting and remote cameras) o guide remote camera placement. 10 Tammar scats from Middle Island, Recherche Archipelago (1cm grid) Tammar scats from Nowanup (1cm grid) Western Grey Kangaroo scats (1cm grid) Unknown scats (1cm grid) 2cm Black-gloved wallaby scats Figure 2: Comparison of the size and morphology of scats of the three Macropod species present in the area. Arrows indicate scats that look similar. Limitations: Tammar scats can be very sparse, and often hard to find (based on searches at sites where they are known to occur), especially if the population is at low densities. The age of scats is hard to determine, so that a distinction between “past” or “current” occupancy cannot always be made. 11 Costs: No initial equipment cost No equipment running cost Low labour intensity – 1 person for 1 hr per site Vehicle hire - 1 day for 5 sites Footprints Size differences occur between the three Macropod species present in the area (Figure 3), however there is possibly an overlap in sizes between adult Black-gloved wallabies and young Western Grey Kangaroos, and between young Black-gloved wallabies and Tammars. Young Black-gloved wallabies that are out of the mothers pouch will only be present in the population between October and January and by February should be close to adult size. Young Western Grey Kangaroos can be present in the population at any time of the year. Some differences occur in the shape of the foot between the three species. The toe to short pes ratio is smallest in Tammars (c.1:1.4), compared with c.1:1.6 in Black-gloved wallabies, and c. 1:2.0 in Western Grey Kangaroos (ie. Tammars have longer toes in relation to their foot than Western Grey Kangaroos) (Figure 3). Generally, any footprint measuring less than 8 cm short pes length (including nail) is likely to be a wallaby species, and any footprint measuring less than 6 cm short pes length (including nail) is likely to be a Tammar (although these latter could still possibly represent a young Black-gloved wallaby if observed between approximately October and January). Combined with other information (eg. known presence, habitat type, scats) an assessment can be made about which species the footprint is likely to represent, therefore this technique has some use in determining presence. Footprints can be used to: o provide reasonable confirmation that animals are still present at a known occupied site o provide an initial quick assessment of possible occupancy by a wallaby species o target areas where more definitive survey techniques can be employed (spotlighting and remote cameras) o guide remote camera placement Limitations: Very clear prints are needed to determine differences in size and shape. Clearer prints are produced in wet conditions rather than dry and on clayey soils rather than sandy. Footprints can generally only be seen on tracks or bare areas, so these must be present at a site in order to use this method. Footprints are often very sparse and hard to find. 12 Costs: No initial equipment cost No equipment running cost Low labour intensity – 1 person for 1 hr per site Vehicle hire - 1 day for 5 sites 13 a) Size differences bewtween the short pes of the three Macropod species c) b) Western Grey Kangaroo foot (right) and footprint (left), toe to short pes ratio c. 1:2.0 c) Tammar foot (right) and footprint (left), toe to short pes ratio c. 1:1.4 d) Black-gloved wallaby foot (right) and footprint (left), toe to short pes ratio c. 1:1.6 Figure 3: Comparisons between the size and morphology of the foot (short pes) and footprints of the three Macropod species present in the area. 14 Remote hair collection An additional detection technique of remote hair collection was trialed in 2009. These trials were conducted at sites where Tammars or Black-gloved wallabies were known to occur (previously detected by spotlighting or remote cameras). Hair can be identified to species using a number of characteristics visible under a monocular microscope (x 40 magnification) (Triggs and Brunner 2002). Due to time constraints and other priorities, limited time was given to these trials (they were trialed at 18 locations). All the remote hair collection techniques provided limited success in detecting Tammars where they were known to occur (4 out of 18 sites) but no success in detecting Black-gloved wallabies (no hairs collected at any site). This technique has potential but will need to be trialed further before it can be successfully applied. The following hair collection traps were trialed: 1. Hair arches (Figure 4)- these consisted of a piece of thick plastic bent over into an arch and secured into the ground with wire, and baited with peanut butter and apple. Hair arches have been used successfully for remote hair collection in small mammals (Mills et al. 2002), however they had limited success in hair collection of wallabies, likely because the animals are too large for their snout to fit under the arch. Out of 981 hair trap nights wallaby (Tammar) hair was collected on only two traps. Hair arch Figure 4: A Tammar inspects a hair arch at the Nowanup NE corner site, captured on remote camera. 2. Fence tape (Figure 5)- this consisted of wrapping double-sided tape around the bottom wire of a fence where it was apparent that an animal was moving under the fence. Out of three known Tammar sites, likely Tammar hair was collected at all three sites via this method. 3. Vertical sticky poles (Figure 5)- these consisted of thin poles in the ground wrapped with double sided tape, placed in areas where animals were thought to be moving. A bait (apple and peanut butter) was placed next to the pole to attract animal to brush against the pole. Out of two known Tammar sites, likely Tammar hair was collected at one site via this method. 15 Fence tape Vertical sticky pole Figure 5: A Tammar about to move under a fence at the Nowanup NE corner site, captured on remote camera. 4. Horizontal sticky poles (Figure 6)- these consisted of a length of plastic pipe strung at approximately wallaby chest height between two trees or between two fence droppers placed in the ground. Double-sided sticky tape was placed on the underside of the pipe. Bait was placed under the pole so that the animal would brush against the tape when retrieving the bait. Out of two known Tammar sites, likely Tammar hair was collected both sites via this method. Figure 6: A horizontal sticky pole fixed between two moort trees at the Corackerup NR southern boundary Tammar site. Further trails should involve developing the most efficient use of these traps (that which provides the best detection rate), by determining: the optimum density and number of traps at a site for animal detection the most appropriate trap type in different situations 16 captive animal (or remote camera video footage) trials to determine animal behavior in taking bait from a trap Limitations: Require animal to be attracted to bait and to take bait in a manner that leaves hair on the tape. In wet conditions the sticky tape had limited “stickiness” (2-3 nights). This was more so if the tape was exposed as in fence tape and vertical sticky poles. Fence tape can only be used where there is a fence on the site that animals appear to be moving under. Costs: Low initial equipment cost (c. $10 per trap for pipe and star pickets) Low equipment running cost (cost of sticky tape, $20 per roll) Medium labour intensity – 1 person for 2 hrs per site (20-30 traps) plus hair identification (2 samples per hour) Vehicle hire- 1 day for 3 sites 2. Direct observation Spotlighting Spotlighting from a vehicle is used extensively for detecting or determining the abundance of Macropods (Southwell 1989). Spotlighting was used in this project: for detecting both wallaby species during 2007 to provide initial estimates of their distribution. during the monitoring sessions at the long-term monitoring sites in Spring 2008 for detecting both species, to a limited extent during the non-monitoring season in 2009 specifically to locate new populations of Tammars. Despite its extensive use, spotlighting has many limitations and the following apply specifically to detecting wallaby species during this project. Limitations: Weather conditions-these have been proved to affect detectability or actual emergence of animals (Southwell 1989). However, based on observation only, the weather conditions did not appear to affect sightings of Black-gloved wallabies (sightings of Tammars via spotlighting was too low to comment). 17 Access- can only be employed in areas where vehicle access is possible, and only in dry soil conditions due to the risk of the spread of phytophthora dieback, specifically on DEC reserves and GLink properties. Local Shire imposed vehicle movement bans also can occur during hot weather. Observer error- due to observer error in detecting species the same observer should be used for each spotlighting session in order to make valid comparisons between seasons or sites. However, this often proved difficult to enforce as volunteers were often too inexperienced to act as driver, therefore taking the role of observer and leaving the project officer the task of driver. As a result the observer was not always consistent. Visibility – whether the track passes through or adjacent to open areas (eg. pasture) or whether it is though thick high remnant vegetation can effect detection. Crops that are late in the season and are up to a metre high can make detecting wallabies difficult. Revegetation and rergrowth can also hinder visibility. For example at both Chingarrup and Chereninup spotlighting is difficult as the track is bordered by 1-2 m high vegetation. This will become a problem at, for example, Peniup Creek Property in about 5 years time when the revegetation reaches a certain density and height. Costs: Medium initial cost (c. $400 for spotlight) No equipment running cost High labour intensity - 2 people for 2 hrs per site, overnight trip required. Vehicle -hire- 1 day (overnight) per site. Remote sensing cameras Remote cameras are increasingly being used as a non-invasive technique to detect the presence and sometimes abundance of a wide range of fauna (Claridge et al. 2004). See photos front page for examples of images captured during this project. Remote sensing cameras were used in this project: as a technique for detecting both wallaby species in 2007 to provide initial estimation of their distribution; during the monitoring sessions at the long-term monitoring sites in Spring 2008 for detecting both species, and during the non-monitoring season in 2009 specifically to locate new populations of Tammars. Advantages: Can be left in place for a long period of time (only limited by battery life or memory card capacity). Are easy to install, if site has vehicle access. Can be installed away from vehicle access. Do not require personnel to be present to monitor, therefore do not require an overnight field trip. 18 The “Beast” remote camera set up on a star picket in Moort woodland. Photo Amanda Keesing. Setting out bait in front of the PiXController Digital Eye remote camera on Eddy and Donna Wajons property Chingarup. Photo Eddy Wajon Despite their increasing use, remote cameras have some limitations and the following apply specifically to detecting wallaby species during this project. Limitations: Reliability of camera function o Two brands of remote cameras were used. A Perth made model (Beast) and a DigitalEye from PixContoller (US). On a number of occasions a camera was non-functional during a monitoring session (one camera at two of the 18 monitoring sites in the Spring 2008 monitoring season), due to such things as flat batteries. o The DigitalEye cameras stopped functioning all together on three occasions and on two occasions was fixed locally, but on one of these occasions it had to be sent back to US for repair and was therefore was out of action for 5 weeks. o Both cameras experienced excessive false triggering in hot, windy weather due to the sensing of moving trees and to hot conditions triggering the temperature sensor. Difficulty of handling- carrying remote cameras to sites away from vehicles was difficult as the cameras needed to be secured onto large star pickets which are quite heavy. Only two cameras and two star picket could be carried at once by one person. 19 Costs: High initial cost (c. $600 per camera) Medium equipment running cost (9v batteries- c. 1 per camera per month) Medium labour intensity - 1 person for 1 hr per camera installation, no overnight trip required. Vehicle hire- 1 day per 2 sites. Table 2: Summary of detection methods Method Signs Direct observation Scats Footprints Hair Spotlighting Remote cameras Definitive presence N N Y Y Y Effort Low Low Med High High Costoverall Low Low Low Med High Detection rates for direct observation methods (spotlighting and remote cameras) As the main priority of the project was to locate populations of animals, time constraints dictated that limited time was available for determining detection rates which require multiple sampling at sites where animals were known to occur. However, limited data was obtained which could be used to calculate detection rates for Tammars. This analysis was carried out by Ayesha Tulloch (UQ). Preliminary results indicate that spotlighting provides a better detection rate than cameras (effort vs result): Mean detection rate for cameras (based on all sites with known tammar occurrence) = 0.10 Mean detection rate for spotlighting (based on all sites with known tammar occurrence) = 0.34 In addition to these calculated detection rates, observations can provide some insight into the efficiency of these methods. Table 3 provides a summary of remote camera results (raw data) at sites where Tammars were ultimately detected. The four sites where more than one monitoring session was required before an animal was detected (in bold) can provide some information on the efficiency of cameras in detecting Tammars. At all these sites, more cameras or more nights were the difference between non-detection and detection of an animal. 20 Table 3: Remote camera results for sites where Tammars were ultimately detected. Sites in bold required more than one monitoring session before an animal was detected. Site No. No. nights cameras No. camera nights Night first detected McGowans Property Corackerup NR East Pennas Nowanup NE corner 1 4 4 1 2 5 1 4 9 3 2 4 Monjebup Peniup – northern fork Millards Property Corackerup NRsouth Peter and Dev Moirs Property Chereninup TOTAL 1 No. nights detected (%) 4 (100) No. cameras detected on (%) 1 (100) 2 1 1 (50) 1 (100) 2 1 3 3 7 2 15 10 1 12 27 21 4 60 1 0 0 1 5 0 4 1 (50) 0 0 3 (100) 2 (28) 0 (0) 4 (26) 1 (20) 0 0 3 (33) 2 (66) 0 (0) 2 (50) 3 9 27 2 1 (11) 1 (33) 1 4 4 1 4 3 2 1 6 1 8 7 2 4 24 1 32 21 0 0 3 0 2 6 0 0 1 (16) 0 3 (37) 2 (28) 0 0 1 (25) 0 1 (25) 1 (33) In addition there were a number of sites where either Tammars or Black-gloved wallabies were known to occur (sighting) or where were highly suspected to occur (anecdotal information or scats detected), but no animals were detected with remote cameras. At Stockwell Rd, Tammars have been seen by the adjacent landholder, however no Tammars were detected in over 50 camera nights. At six sites where wallaby scats were detected and one camera had failed to detect an animal, four cameras were installed but no Tammars or Black-gloved wallabies were detected. At Fred Powell’s Stock Rd property the regular roo shooter reported seeing Tammars intermittently. Wallaby scats were also detected but an animal was not detected in 49 camera nights. Black-gloved wallabies are easier to see during spotlighting than Tammars due to differences in behavior. Black-gloved wallabies tend to venture further out into pasture and will sit still once spotlighted before moving away (sometimes don’t move away at all), Tammars however move very quickly into neighboring bush, leaving little time for positive identification. 21 Of the six monitoring sites where Black-gloved wallabies were ultimately detected during the Spring 2008 monitoring season, animals were not detected by spotlighting at all at two of these sites. Of the remaining four sites, three had detections on one out of the three nights and one on two out of the three nights. Animals were only detected by remote cameras at two out of the six occupied sites. This indicates that a combination of detection methods is preferable for increasing detection rates for this species. 5. SUMMARY OF RESULTS – MARCH 2007 to DECEMBER 2009. All data gathered over the course of the project is stored in an Access (2007) database entitled “Wallaby database”. The following is a summary of the results obtained. A total of 61 Tammars and 237 Black-gloved wallaby sightings were obtained over the three years. Of these 47 Black-gloved wallaby sightings were obtained during the Spring 2008 monitoring sessions at the permanent monitoring sites, but no Tammars were observed. One hundred and ninety Black-gloved wallaby and 61 Tammar sightings were obtained during surveys and opportunistic observations outside of the monitoring sessions at the monitoring sites (Table 3, Figure 1). The baseline level of occupancy for Black-gloved wallabies of 0.3666 (+/-0.0754) (PRESENCE program, McKenzie et al. 2002) was established during the Spring 2008 monitoring on permanent monitoring sites. The level of occupancy for Tammars in the first monitoring season was zero; it was not detected on any site via either spotlighting or remote cameras. During targeted Tammar surveys, they were detected at five of fourteen sites , increasing the total number of sites known to be currently occupied by Tammars in the Fitz-Stirling to 12 (Figure 1). The larger number of records obtained throughout the project for Black-gloved wallabies, although based largely on opportunistic sightings, likely reflects a combination of the greater numbers and distribution of this species but also possibly its higher detectability in comparison to Tammars. The occurrence of the two species on GLink properties is summarised in Appendix D. Table 4: Summary of the number of Tammar and Black-gloved wallaby sightings over the three years of the project. Monitoring Non-monitoring (Opportunistic) TOTAL Number of Tammar sightings 0 61 Number of Black-gloved wallaby sightings 47 190 61 237 22 6. MONITORTING PROTOCOLS FOR TAMMARS AND BLACK-GLOVED WALLABIES A combination of the above detection techniques can be employed to determine the presence of Blackgloved wallabies or Tammars at a site. Certain methods can be more effective in certain situations and therefore a combination of methods should be employed to determine the status of a site as occupied or unoccupied. The assumption for these detection methods are based on the following resources: Four remote cameras (currently available). One vehicle. One paid personnel, at least one volunteer for spotlighting. a) Black-gloved wallabies i. Detection methods Detection methods for Black-gloved wallabies largely follow the same methods as used in the Spring 2008 monitoring season (Gilfillan 2008b), as follows (additions or changes in bold). 1. Each site is surveyed over 3 consecutive nights using two non-invasive survey techniques; spotlighting from a vehicle and remote cameras, with two sites being surveyed simultaneously. 2. At each site spotlighting is carried out over three consecutive nights, covering the entire length of the established spotlight route each night (maps available). Most spotlight routes follow the vegetation / pasture or crop interface, but some routes encompass tracks through remnant vegetation patches. Spotlighting is carried out by shining the spotlight (50 Watt globe- a red filter can be used if prolonged shining onto the animal is needed) from the window of the vehicle, into the pasture or crop if possible. The vehicle travels at c. 20-30km an hour. A GPS coordinate (UTM Zone 50) is taken of each wallaby seen. All other animals seen are counted only (except for rabbits). Weather conditions on each night are also recorded. In addition the estimated distance that an animal is seen at a perpendicular angle to the vehicle should be recorded (See Section on TripWatch) 3. At each site two remote cameras are placed at permanently marked sites which are within a 500m radius of the site point location, and at least 500m apart. Markers are star pickets labeled with the remote camera site code (see Appendix A). Cameras are checked every day, as problems with malfunctioning batteries or large numbers of false triggers meant that they cannot be reliably left for three days without checking. 23 4. Sites are considered occupied on a particular night if a species was captured on either of the remote cameras, or if observed spotlighting within 1km of the site point location (an alternative is to determine occupancy based on the position of animal in relation to the site- see section 2 above) 5. In addition to spotlighting and remote cameras, a track and scat search is carried out at each site. Scats are collected in four 100 transects radiating out at 90 degree angles from each of the remote camera sites. Each transect is given a reference based on the compass direction pointing away from the camera site. The transect is walked, and any probable wallaby scats seen from the transect collected and placed in paper envelopes. Separate scats, or groups of scats, are placed in separate paper bags, and the transect reference recorded. Any footprints measuring less than 8 cm are also recorded along each transect (present or absent). As further funding becomes available then the priorities should be: purchase of additional remote cameras (at least 12 at $600ea) faecal DNA analysis for species identification in order to increase detection rates. ii. Monitoring site design and review of FLP target and objective The successful detection of Black-gloved wallabies using the methods and monitoring site design employed in Spring 2008 indicates that this monitoring protocol should be maintained for this species. The FLP objective of “By 2017, increase the populations of Tammars and Black-gloved Wallabies within the Fitz-Stirling area by 30%.” is also a reasonable objective for this species and due to the fairly widespread occurrence of this species should be able to be measured successfully (by occupancy rate). However the use of this species as a target for measuring the impact of fox control should be reviewed. Although this species is less abundant than it was 30-40 years ago (anecdotal evidence) it still maintains a relatively high level of abundance and a fairly widespread distribution. It almost certainly suffers from fox predation on some level (young animals are known to be taken), but it does not appear to have been impacted by fox predation to the degree that Tammars have (Kinnear et al. 2002). This species is a suitable target however to measure increased habitat occupancy through habitat restoration and increased connectivity. Being relatively widespread and occurring in vegetation types that are quite common across the landscape, and given that it has already been observed using revegetation for both moving through and resting its expansion onto currently unoccupied suitable habitat will almost certainly be enhanced by habitat restoration and increased connectivity. iii. Recommendations 1. The monitoring site design for Black-gloved wallabies should follow the same as that used in the Spring 2008 monitoring season (Gilfillan 2008b). Now that a baseline level of abundance (site occupancy) has been established the frequency of sampling can be carried out every two - five years. It is not necessary to sample all sites in one year, so that half or a third may be able to be sampled in one monitoring session and the remaining in subsequent monitoring sessions. 24 2. TripWatch In addition to the permanent monitoring sites a “program” called TripWatch should be introduced. TripWatch is a means of recording animals seen at any time on regular trips throughout Gondwana Link’s Fitz-Stirlings Operational Area. TripWatch can be carried out by anyone who drives throughout the area or on certain routes on a regular basis. As long as some simple rules are observed (see recording sheet in Appendix B) over a long time period of repeated observations on the same routes, valuable information on species abundance, distribution and habitat preferences can be gained. Personnel working in the area on a regular basis and any interested members of the community can be encouraged to fill out the TripWatch recording sheet. 3. Maintain the current FLP objective for this species but use it only as an indicator of increased habitat occupancy through habitat restoration and increased connectivity and not as an indicator for measuring the impacts of fox control. 4. As further funding becomes available a. Consider expanding the monitoring sites beyond the Corackerup Sub-catchment. 25 Former SFMA Properties Figure 7: Locations of permanent monitoring sites and their occupancy status in Spring 2008. GPS coordinates are given in Appendix 1. 26 b) Tammars i. Detection methods Detection methods for Tammars largely follow the same methods as used in the Spring 2008 monitoring season (Gilfillan 2008b), as outlined above. However, due to the patchy nature of Tammar distribution, where groups of animals appear to use a fairly small area of suitable habitat, detection methods need to be more targeted, therefore the following changes apply: Remote cameras Placing cameras randomly at a site will unlikely result in detection of an animal. Therefore, cameras should be placed where there is additional evidence of the species (scats or tracks). If no such indirect evidence is present then cameras should be placed in patches of potentially suitable habitat. Rather than using only two cameras per site at 500m apart, all four cameras should be placed in relatively close proximity to each other (within 100-200m). This dictates that only one site can be monitored at a time under the assumption of four available cameras. Optional method- Remote hair collection Tammars were detected (although at a low rate) with remote hair traps. These could be used in certain situations, for example in the absence of a sufficient number of cameras, where limited monitoring funds are available , or where vehicle access is limited and cameras cannot be efficiently carried on foot. Sticky horizontal poles appear to be the most successful in obtaining Tammar hair (based on limited trials- see above). These could be randomly placed throughout the site in large numbers (20-30). This method is not at a stage to be used as part of the standardised monitoring protocol at a permanent monitoring site, but the potential exists for their use in this situation once their efficiency has been determined???. It should be noted, although inexpensive, they are nearly as time consuming to install as remote cameras. When further funding is available then priorities are: purchase of additional remote cameras (12 at $600ea) faecal DNA analysis for species identification in order to increase detection rates. ii. Monitoring site design and review of FLP target and objective A robust estimate of Tammar abundance was not obtained with the design of the Spring 2008 monitoring sites, due the apparently very patchy nature of Tammar populations across the landscape in relation to the density and distribution of monitoring sites. This species therefore requires an alternative monitoring site design. In addition, while Tammars should be maintained as an indicator for measuring the impact of fox control as they have been shown to respond to fox baiting (Kinnear et al. 2002), their occurrence as groups occupying small patches of habitat (which may be quite uncommon) suggest that 27 they may not be a suitable target for measuring impacts of habitat restoration (the type of habitat being restored may not be suitable for Tammars) and increased connectivity. Keeping the Tammar as an indicator for the impacts of fox control, the FLP objective of “By 2017, increase the populations of Tammars (and Black-gloved Wallabies) within the Fitz-Stirling area by 30%.” should be reviewed. Although after targeted surveys for Tammars in 2009 a reasonable baseline of current distribution now exists, this was as a result of intensive searching in areas where the species was suspected to occur. A 30 % increase in abundance in the whole population across the landscape from this baseline is not easily or accurately measured under the current survey methods available and would require a very large number of sites (50-100) placed randomly or stratified randomly across the Fitz-Stirling area, which would be very labour intensive. iii. Recommendations Options for monitoring site design. The following options are recommended for potential monitoring site design for Tammars. 1. Concentrate monitoring sites initially on Chereninup only, as per Bush Heritage’s WA State NRM funding proposal (January 2010). (Monitor two known currently occupied sites, one on the western edge of the large remnant on Pennas property and one on the southern edge of the remnant on Chereninup, plus 6 sites with potentially suitable habitat for Tammars but currently unoccupied). 2. Set up sites across the whole of the Fiz-Stirling. A useful case study for sampling design for species with clumped distributions has been outlined by Witczuk et al. (2008). The following design is a modification of this. a) 5-10 sites - currently occupied, monitored to test for continued occupancy. b) 5-10 sites- not currently occupied but with historical occupancy. c) 5-10 sites - random selection of sites within potentially suitable habitat but not currently occupied and no historical evidence of occupancy. The latter two groups of sites are to test for new colonisation or expansion. Group a) sites will need to be chosen using the completed habitat model produced by Ayesha Tulloch. Each unoccupied site should have no physical barriers to dispersal (large expanses of cleared habitat) between them and a currently occupied site. A subset of monitoring sites would only need to be sampled per monitoring session (yearly or biannually) therefore allowing a greater number of sites in total. It is important to note that because it is not based on a random or stratified random (probabilistic) selection of sites, if this design is used then conclusions can only be made about the sites being monitored and no inferences can be made about the wider population (Elzinga 2001). Incorporating the use of existing sites 28 A number of existing sites could be incorporated into this design. WA18-occupied WA01- occupied WA17 - within 1.3 km of occupied site (no barrier to dispersal) WA10 – 3.7 km from an occupied site (no barrier to dispersal) Newbey’s Eucalyptus flocktoniea vegetation association sites (WA01, WA02, WA03) and Newbey’s Eucalyptus platypus (Moort) vegetation association sites (WA17, WA18) (or those sites found to be suitable by Ayesha Tulloch’s habitat model) – the advantage being that these sites have already been monitored once so that their occupancy status is already known. 3. An alternative approach would be to maintain the 30% increase objective but to measure changes in abundance at known sites. It has been suggested that abundance may be better measurement for management as changes in presence –absence may not be detected until a catastrophic decline has occurred (Field et al. 2005). This could be supplemented by a number of sites (5-10) in potentially suitable habitat but not currently occupied where presence or absence only is measured to test for new colonisation or expansion. This approach would require the use of either faecal DNA (determination of population numbers by the identification of individuals) or possibly by faecal pellet counts. The latter would require faecal deposition and decay rates to be determined. Possible alternatives for FLP objective Tammars should be maintained as an indicator for measuring the impact of fox control only. The objective should relate more specifically to the monitoring site design. For example if option 2 is adopted then the objective could be:“Expansion into 50% of suitable unoccupied monitoring sites under a fox control regime” 29 7. APPENDICES Appendix A: Spring 2008 monitoring site codes and GPS coordinates Monitoring site WA01 WA02 WA03 WA04 WA05 WA06 WA07 WA14 Location Tenure Corackerup NRDEC southern boundary Private Property (Kent Private Loc 1497), B and G Cake- Cnr of SC Hwy and Cowallelup Rd. Peniup NR, centre DEC Former SFMA property SFMA - Glen Orchids Private Property (Kent Private Loc 1828)- Greg Hyde Chingarup Private Easting Northing Contact Comment 652930 6212117 DEC (Sarah Comer, Peter Collins or Greg Freebury - 98424500) 665924 6210842 B and G. Cake (Greg Cake) 98361042 (0427361042) 667094 6217664 DEC (Sarah Comer, Peter Collins or Greg Freebury - 98424500) 676736 6235651 Unknown 656303 6226920 Owner-Lullfitz, leased to Greg and Sarah Hyde-98353047 656397 6203034 Eddie Wajon (wajonpub@wantree.com.au) UCL and Private 654664 6200534 UCL (DEC)/Peter Moir 98472058 (0427472058). UCL Corackerup Crk, adj. to Borden/Boxwood Hill Rd- adj Private Property (Peter Moir (Kent Loc 1881)) Fred Powell’s Private Cowellelup Rd Property (Ken Loc 1841), western boundary adj to Dixon 653831 6220699 Fred Powell (0427351096, 98351096) 30 Monitoring site WA08 WA09 WA10 WA11 WA12 WA13 WA15 WA16 WA17 WA18 Location Tenure property Peniup NR- western DEC boundary Tim Foster- Cnr Stock Private Rd and Cardininup Rd. (Kent Loc Chereninup Reserve Bush Heritage Crown Res and PPCorackerup Rd, N of Boxwood/Ongerup Rd. (Kent Loc 1827 and 1209) Peniup NR- SE cnrboundary with Greg Cake (Kent Loc. 1902) Terry Walters Property (Kent Loc 1413) Peniup Creek Property UCL/Private Easting Northing Contact Comment 664682 6225070 DEC (Sarah Comer, Peter Collins or Greg Freebury - 98424500) 660519 6234173 Tim Foster (98282124, NOT DONE- site not suitable0428367837)- lives off property remnant now partly cleared 662421 6222516 Bush Heritage (inform Mal Graham of visitmalgraham@westnet.com.au) 652481 6230305 Crown Reserve/Graham Jones 98353013 (to east- 1209) and Greg Hyde (to west- 1827) 98353047 DEC/Private 671529 6214329 DEC/Greg Cake (98361042 or NOT DONE- access difficult from 0427361042) for access from south south Private 671743 6232980 Terry Walter (98351147) Greening/Bush 672544 6227674 Greening Australia (Corrie Watts 08 Heritage 9335 8933) Bush Heritage (Jeff Crane- 98362026) Former SFMA property SFMA 667022 6236788 Unknown - South Yatelands Nowanup- SW corner- Greening 658778 6210074 Greening Australia (Corrie Watts adjacent to UCL on (93358933) Corackerup Ck Corackerup NRDEC 658062 6216315 DEC (Sarah Comer, Peter Collins or Normans Rd. Greg Freebury - 98424500) boundary, adjacent to Merrigup Creek crossing 31 Monitoring site WA19 WA20 Location Tenure Easting Northing Contact Comment Privite Property -R.J. Private/SFMA 672200 6238319 Former SFMA- unkown. Russell (Kent Loc R.J.(Ron)and Erica Russell 1402), and Former (98351108) SFMA propertiesTantanoola and Ankayarra Peniup Creek Property Greening/Bush 671949 6224070 Greening Australia (Corrie Watts Heritage 93358933), Bush Heritage (Jeff Crane- 98362026) 32 Appendix B: Spatial datasets relating to the project File Name Custodian Date Wallaby recordsgeneral Wallaby_records_all Greening Australia/ Gondwana Link Project duration from March 2007 to December 2009 From 1966 to Decemb er 2009 and ongoing Wallaby recordsmonitoring Wallaby_records_moni toring Greening Australia/ Gondwana Link September 2008 to November 2008 Sep 2008 to Nov 2008 Description Area covered File type Datum Comments Contains all known records of Tammar (Macropus eugenii derbianus) and Blackgloved wallabies (Macropus irma) within the study area collated from past records (other sources) and collected nonsystematically during the course of the study. Contains records of Tammar (Macropus eugenii derbianus) and Black-gloved wallabies (Macropus irma) obtained during systematic monitoring at specified monitoring sites. This dataset is a subset of Wallaby records-general Fitz-Stirling Operational Area plus surrounding area of approximately 50 km Access 2007, Excell 2007 and ArcGis shapefile GDA94 The records are from many sources and have varying levels of accuracy and precision. A subset of records collected only during the project can be created. Corackerup Subcatchment Access 2007, Excell 2007 and ArcGis shapefile GDA94 Level of occupancy percentages have been derived from this dataset. Date of records Title 33 File Name Custodian Date Description Area covered File type Datum Comments Long-term wallaby monitoring sites Monitoring_sites Greening Australia/ Gondwana Link September 2008 to November 2008 set up and first monitoring season Point locations of long-term wallaby monitoring sites Corackerup Subcatchment Access 2007, Excell 2007 and ArcGis shapefile GDA94 Greening Australia/ Gondwana Link September 2008 to November 2008 set up and first monitoring season Locations of remote camera sites at longterm wallaby monitoring sites Corackerup Subcatchment Access 2007, Excell 2007 and ArcGis shapefile GDA94 Remote_camera_sites_ non_monitoring Greening Australia/ Gondwana Link Project duration from March 2007 to December 2009 NA Fitz-Stirling Operational Area Access 2007, Excell 2007 and ArcGis shapefile GDA94 Spotlight_routes Greening Australia/ Gondwana Link Project duration from March 2007 to December 2009 NA Locations of all points where remote cameras were installed outside of the permanent monitoring sites. Polylines of all spotlight routes undertaken over the course of the study The site point location of the long-term monitoring site, which encompasses 50 ha around this point. There are two remote camera locations per longterm monitoring site, each within a 500m radius of the site pint location and at leat 500 m apart. One remote camera is placed at each location. The number of cameras per site is variable. Long-term wallaby monitoring sitesremote camera locations Remote_camera_sites_ monitoring Remote camera locationsnonmonitoring Spotlight routes Fitz-Stirling Operational Area ArcGis shapefile GDA94 Date of records Title A subset of spotlight routes for the long-term monitoring sites can be extracted. 34 Appendix C: TripWatch recording sheet TripWatch is a means of recording animals seen on regular trips throughout Gondwana Link’s Fitz-Stirling Operational Area It can be carried out by anyone who drives throughout the area or on certain routes on a regular basis. As long as some simple rules are observed (see below), the observations can be used to gain valuable information on species abundance, distribution and habitat preferences. To be used for: Tammar, Black-gloved wallaby, Fox and Feral Cat FIRST FILL OUT THE DETAILS OF THE ROUTE TAKEN (ATTACHED ROUTE SHEET) FOR EACH ROUTE TAKEN: IF NO ANIMAL IS SEEN THROUGHT THE ROUTE THEN PUT A CROSS HERE____ (NB: A ZERO record is as important as a sighting) IF AN ANIMAL IS SEEN THEN RECORD THE SIGHTING (S) ON THE ATTACHED RECORD SHEET USING THE FOLLOWING INSTRUCTIONS: 1. DRIVE TO POINT WHERE YOU ARE AT RIGHT ANGLES TO WHERE THE ANIMAL WAS FIRST SEEN ( AND RECORD THE DISTANCE FROM HERE ( ) TO THE ANIMAL (A). ) ESTIMATE A CAR Line of sight 2. RECORD THE GPS COORDINATE (EASTING / NORTHING), OR MARK ON THE MAP THE POSITION OF 3. RECORD THE DIRECTION OF THE SIGHTING FROM THE ROAD/TRACK (Left or right) 4. RECORD THE TIME OF THE SIGHTING 35 FITZ-STIRLINGS TRIP WATCH - ROUTE DETAILS Start a new sheet for each separate route OBSERVER:___________________________________________________________________ ROUTE: (eg. Peniup to Chereninup via Marningarup and Carney Rds), OR DRAW THE ROUTE ON THE MAP PROVIDED. ______________________________________________________________________________ ______________________________________________________________________________ DATE___________________________TIME (24hr clock): START____________________FINISH___________________ WEATHER CONDITIONS (if night, then include moon status)_______________________________________________________________________ _____________________________________________________________________________ ADDITIONAL COMMENTS:__________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ A series of maps with aerial photo of whole of Fitz-Stirlings with roads, reserves and reserve tracks will accompany this sheet. 36 FITZ-STIRLINGS TRIP WATCH – RECORD SHEET USE SEPARATE RECORD SHEET FOR EACH ROUTE TAKEN Observation number Mark on the map with a dot and the observation number 1 Species seen Number of individuals seen Time (24 hour clock) Estimated distance (m) at right angles from direction of travel when first sighted (“A” in diagram) Direction animal seen from transect, (L) left (R) right Easting of Northing of 2 3 4 5 6 7 8 9 10 11 12 13 37 Appendix D: Wallaby occurrence and survey effort on GLink Properties The following is a summary of the status of Tammar and Black-gloved wallabies on Bush Heritage and Greening Australia Properties within the Fitz-Stirlings Operational Area of Gondwana Link. Specific locations of records for each property can be seen in Figure 1 in the body of the report. Suspected population (indirect sign) or record in close proximity Black-gloved wallaby Tammar Property Confirmed population (detected by spotlighting or remote camera) BlackTammar gloved wallaby Yarrabee Yes No - - Monjebup Monjebup North Nowanup Chereninup Peniup Creek No No Yes Yes Yes Yes No Yes Yes No Yes Yes - Yes Possible Yarrabee No permanent monitoring sites exist on this property. Survey effort Cameras-50 camera nights Spotlighting-1 night Hair poles- 6 for 3 nights Tammar No tammars have been detected on Yarrabee to date Survey effort was low No apparently suitable habitat exists (Ayesha) One record (a roadkill) occurs for the corner of Gnowellan and Kuch Rds in 2001. This may have been soon after an extensive wildfire in the adjacent Stirling Range NP so the animal could have travelled quite some distance ahead of the fire. Stirling Range NP- no evidence (indirect) was found during one search of the NE corner of the park, and no apparently suitable habitat was located, however status for this area of the park is still unknown. Black-gloved wallaby 38 Have been observed throughout Yarrabee; within regrowth, in the revegetation and along the Gnowellan Rd boundary. The animal in the revegetation was flushed from a Juncus pallidus clump within a large patch of Juncus during the day indicating that it was using this plant as a daytime shelter. All observations have been opportunistic sightings. Remote hair collection was trialed at the revegetation site but no hairs were collected. Four remote cameras were placed for three nights in the Jarrah woodland adjacent to the revegetation where animals have been observed opportunistically, but no animals were detected by this method. Monjebup No permanent monitoring sites occur on this property. Survey effort Cameras-21 camera nights (NE corner); 12 camera nights (near October 2008 campsite) Spotlighting-1 night (along southern central boundary) Remote hair collection - none Scat/footprint search- on 2 occasions on the southern central boundary, on one occasion adjacent to Monjebup Rd. Tammar Tammars have been detected in the NE corner, near Monjebup Rd. via remote camera. Black-gloved wallaby No records exist to date for Black-gloved wallabies Probable scats were found in SE corner The closest record occurs on Monjebup Rd. c 2km north of Monjebup Property Monjebup North No permanent monitoring sites occur on this property. Survey effort Remote cameras- 48 camera nights Spotlighting-1 night Remote hair- none Scat/footprint/ habitat search- 1 occasion Monjebup North was purchased in late 2009, only a few months prior to the current project end date, therefore survey effort has been minimal to date. 39 Tammar Tammars have not been detected to date. Tammars occur within Monjebup near the boundary of Monjebup Nth, therefore it is likely that tammars also occur here. A large area of possibly suitable habitat exists (Moort/ E.flocktoniea) exists, a one day search failed to find scats. Black-gloved wallaby A large amount of apparently suitable habitat exists and this species is likely to be detected with more survey effort. Nowanup One permanent monitoring site occurs on this property (WA17) Survey effort Remote cameras-6 camera nights (WA17); 54 camera nights in the NE corner Spotlighting- 3 nights monitoring; 3 nights non-monitoring Remote hair- yes Scat/footprint habitat search- at NE corner, WA17 and near house. Tammar One population of Tammars has been found in the NE corner, adjacent to the large remnant along Corackerup Creek. Animals have been seen feeding out in the regrowth, retreating into the remnant consisting of Moort and Eucalyptus flocktoniae association when alerted by spotlight. A small macropod was also seen on one occasion on the track just south of the house. The animal was too small for a Black-gloved wallaby and may have been a Tammar, although 27 camera nights and 5 spotlighting sessions have failed to detect an animal to date. Black-gloved wallaby Black-gloved wallabies have been observed at a number of localities throughout the property, with opportunistic sightings contributing to distribution knowledge, particularly in the area north of the house and in the revegetation. Black-gloved wallabies were detected via spotlighting during the Spring 2008 monitoring season. Chereninup One permanent monitoring site occurs on this property (WA10) 40 Survey effort Remote cameras-21 camera nights (southern boundary); 6 camera nights (WA10) Spotlighting- 3 nights monitoring; 1 night ex-monitoring (see map for route) Remote hair- none Scat/footprint search- 3-4 occasions Tammar Tammars have been detected via remote camera on the southern boundary. Black-gloved wallaby Black-gloved wallabies were detected via remote camera during the Spring 2008 monitoring season (WA10C1 camera site). Peniup Creek Two permanent monitoring sites occur on this property (WA15 and WA20). Survey effort Remote cameras-24 camera nights (NE corner and moort); 12 camera nights (WA15 and WA20) Spotlighting- 3 nights monitoring; 7 nights non-monitoring (see map for route) Remote hair- none Scat/footprint searches- on two occasions in moort, specifically for tammars. Tammar The closest current record of a tammar is in Peniup NR, c. 3 km to the south. This site is connected to Peniup Creek Property via remnant vegetation along Peniup Creek. The roo shooter for the property (Jim Jackson) has seen Tammars recently (last two years), in the NE corner of the property, but remote cameras and spotlighting during this project failed to located any animals. Scats found in moort in the centre of the property, near Peniup Creek have been identified as Tammar by Noongar elders (Dortch 2008) and scats of either Black-gloved wallabies or Tammars were detected in this section of moort during this project, however remote cameras and spotlighting failed to detect an animal. Tammars likely occur on this property, or occurred until very recently, and if still present may occur in very low densities making detection difficult. Black-gloved wallaby 41 Black-gloved wallabies have been located in the granite area in the south of the property (WA20) and have been flushed from regrowth adjacent to this area, indicating they are using the regrowth for shelter. They have also been seen near the moort patch near the eastern boundary of the property, where tammars are suspected to occur. 42 8. REFERENCES Alacs, E., Alpers, D., de Tores, P., Dillon, M. and Spencer, B. S. (2003). Identifying the presence of quokka (Setonix brachyurus) and other macropods using cytochrome b analyses from faeces. Wildlife Research 30: 41-47. Bamford, M. J. and Bamford, A. R. (2002). A Study of the Brush or Black-gloved Wallaby Macroups irma (Jourdan 1837) in Whiteman Park. Western Australian Planning Commission., Prepared for Whiteman Park Board of Management. Berry, O., Sarre, S. D., Farrington, L. and Aitkin, N. (2007). Faecal DNA detection of invasive species; the case of feral foxes in Tasmania. Wildlife Research 34: 1-7. Christensen, P. (1980). The Biology of Bettongia penicillata (Grey, 1837) and Macropus eugenii (Desmarest 1817) in Relation to Fire. Forests Department of Western Australia. Bulletin No.91. Claridge, A.W., Mifsud, G., Dawson, J. Saxon, M. (2004).Use of infrared digital cameras to investigate aspects of the social behaviour of cryptic species. Wildlife Research 31 (6). Dortch, J. (2008).Archaeological surveys on a BushHeritage Australia reserve (KentLocation 1419) on Peniup Creek,Shire of Jerramungup, WA Elzinga, C.L. (2001). Monitoring Plant and Animal Populations by Caryl L. Elzinga, Daniel W. Salzer, John W. Willoughby, James P. Gibbs.Published in January 1, 2001, Blackwell Publishing Limited Field, S.A., Tyre, A.J. and Possingham, H.P. (2005). Optimising allocation of monitoring effort under economic and observational constraints. Journal of Wildlife Management 69 (2): 473–482. Gilfillan, S. (2008a). Sampling design for Tammar and Black-gloved wallaby monitoring sites in the FitzStirling Operational Area, Gondwana Link. Gondwana Link/Greening Australia Report. Gilfillan, S. (2008b). Gondwana Link / Greening Australia Wallaby Project. Spring 2008 Monitoring Season. Kinnear, J. E., Sumner, N. R. and Onus, M. L. (2002). The red fox in Australia - an exotic predator turned biological control agent. Biological Conservation 108: 335-359. MacKenzie, D.I., Nichols, J.D., Lachman, G.B., Droege, S., Royle, J.A. and Langtimm, C.A. (2002) Estimating site occupancy rates when detection probabilities are less than one. Ecology 83: 2248–2255. Mills, J.D., Harris, B., Claridge, A.W. and Barry, S.C. (2002). Efficacy of hair-sampling techniques for the detection of medium-sized terrestrial mammals. I. A comparison between hair-funnels, hair-tubes and indirect signs. Wildlife Research 29(4) 379 – 387. Piggott, M. P. and Taylor, A. C. (2003). Remote collection of animal DNA and its application in 43 conservation management and understanding the population biology of rare and cryptic species. Wildlife Research 30: 1-13. Piggott, M. P., Banks, S. C., Stone, N., Banffy, C. and Taylor, A. C. (2006). Estimating population size of endangred brush-tailed rock wallaby (Petrogale penicillata) colonies using faecal DNA. Molecular Ecology 15: 81-91. Southwell, C. (1989). Techniques for monitoring the abundance of kangaroo and wallaby populations. In: Kangoroos, Wallabies and Rat-kangaroos. New South Wales, Surrey Beatty and Sons Pty. Ltd: 659693. Triggs, B. and Brunner, H. (2002).Hair Id: An Interactive Tool for Identifying Australian Mammalian Hair. CD-Rom. Ecobyte Pty Ltd. Witczuk, J., Pagacz, S. and Mills, L.S. (2008). Optimising methods for monitoring programs: Olympic marmots as a case study. Wildlife Research 35(8) 788–797. 44 45