BIO 205
Microbiology with Lab
(Title Change ONLY – Oct. 2013)
Course Package
Approved December 10, 2004
Effective Spring 2005
Modified
April 3, 2009
COURSE INFORMATION
Title
MICROBIOLOGY
Number
BIO 205
Catalog Course Description:
A study of the structure, function, and classification of microorganisms and their
application to industrial, agricultural, and medical problems.
Credit hours
4
Prerequisites
Lecture 3
Lab hours 3
hours
BIO 100, BIO 150, BIO 181 or the permission of the instructor
Contact person(s)
Don Plantz (BHC), Jeff Kingsbury (LHC), Tom McKinney (KGM)
Intended Course Outcomes
Course Goals
1. Students will demonstrate an understanding of microbial growth and
metabolism.
2. Students will demonstrate an understanding of the control of microorganisms
3. Students will demonstrate an understanding of the relationships between
microbes and human society including microbes as disease agents, microbial use in
industrial processes, and microbial use in agriculture.
4. Students will demonstrate an understanding of microbes in the natural world
including microbial classification, the role of microbes in local, regional and global
ecology, and in their evolved interactions with other organisms.
5. Students, in the laboratory, will demonstrate safe techniques used to manipulate
microbial growth and some of the techniques used for the identification and
classification of microorganisms.
Course Competencies and Objectives
Competency 1 To distinguish between the various groups of microorganisms and
discuss, where appropriate, the ecological, industrial, agricultural, and medical
aspects of each group.
Objective 1.1 Identify specific groups and subgroups of each microbial taxon.
Objective 1.2 List and discuss characteristics of selected microorganisms that
make them useful in ecological, industrial, agricultural and medical processes.
Competency 2 Demonstrate an understanding of microbial metabolic processes,
the importance of these in microbial activities to us and in the natural world and
their use in the identification of microorganisms.
Objective 2.1 Using previously gained knowledge of metabolic processes,
compare microorganisms to other organisms and indicate how the microbial
processes and products differ from those of most multicellular organisms.
Objective 2.2 Use the unique aspects of microbial metabolism to identify
selected microorganisms in the laboratory setting.
Competency 3 Demonstrate an understanding of microbial grtowth patterns, the
ecological factors affecting these patterns and a variety of mechanisms for
controlling bacterial growth.
Objective 3.1 Draw the bacterial growth curve and explain what is occuring in
each stage of growth.
Objective 3.2 List the ecological factors which control microbial growth and
indicate how the pattern is changed as each factor changes
Objective 3.3 Discuss the materials and processes commonly used by humans to
control the growth of microorganisms.
Competency 4 Demonstrate an understanding of bacterial genetics and
recombinant DNA technology as it relates to microorganisms.
Objective 4.1 Illustrate the mechanisms of genetic information transfer from one
individual to another
Objective 4.2 Outline the process by which genetic information is modified
through recombinant DNA technology.
Objective 4.3 Provide examples of genetically modified organism.
Objective 4.4 List and discuss the health and environmental concerns regarding
recombinant DNA technology
Competency 5 Demonstrate a knowledge of microbial classification and the ability
to identify selected microorganisms in the laboratory setting.
Objective 5.1 Distinguish between and describe characteristics fo each of the
major microbial groups, and discuss the role of those organisms in both the natural
and the human world.
Objective 5.2 Demonstrate laboratory techniques used to identify
microorganisms and use these techinques to identify exanmples of unknown
organisms.
Competency 6 Discuss microbial diseases both generally and specifically and the
vertebrate immune system response to disease and infection including
mechanisms of pathogenicity, disease signs and symptoms, nonspecific and
specific immune responses, and practical immunity.
Objective 6.1 Discuss the structure of the major human organ systems and each
of the major diseases of each of these systems.
Objective 6.2 Describe and discuss how different organisms cause organic
damage to tissue and how this damage modifies organ system structure and
function.
Objective 6.3 Describe the functioning of the vertebrate immune system and
detail how non-self items are defeated by this system.
Objective 6.4 Students will describe immunological tests used to identify
microorganisms and other medical conditions.
Competency 7 Students will understand the role of microorganisms in industry and
agriculture and demonstrate some of these activities in the laboratory setting
Objective 7.1 Detail the use fo microorganisms in producting a variety of
industrial products.
Objective 7.2 Detail microorganisms use to enhance productivity in the
agricultural setting.
Objective 7.3 Demonstrate selected industrial/agricultural production process in
the laboratory.
Competency 8 Students will demonstrate an understanding of the role of
microorganisms in ecological/environmental processes in natural settings and
demonstrate these processes (in a limited fashion) in the laboratory setting.
Objective 8.1 Discuss the role of microorganisms in biogeochemical cycles, as
sources on energy in terrestrial and aquatic food webs, and their importance in
natural and human originated bioremediation.
Objective 8.2 Use selected laboratory tests to demonstrate the role of bacteria in
maintaining environmental quality and to assess microbial activity in the
environment.
Teacher’s Guide
Course Materials and Equipment
Textbook(s)
Software
Title
Author(s)
Publisher
ISBN
Microbiology, An Introduction (Tenth Edition)
Tortora, Funk, Case
Pearson
0-321-55007-2
Title
Publisher
ISBN
Also available as an e-book
Equipment
Other
Learning Units
Learning Unit Topic 1: Introduction to Microbiology
Competency: 1
Objectives: 1.1, 1,2
Activities/ Assignments: Readings-Textbook Chapters 1, 3, 4 and Lab Manual Pages 29-56.
Lecture and class discussions- Introduction to Microbiology, classification of
microorganisms, applied microbiology, medical microbiology.
Media- Blackboard presentation- Introduction to Microbiology, and Basic Microscopy.
Laboratory Exercises- Lab manual exercises 1 and 5-7 , Microscopy and Survey of
Microorganisms
Learning Unit Topic 2: Microbial Metabolism and Biochemistry
Competency: 2
Objectives: 2.1, 2.2
Activities/ Assignments: Readings- Textbook: Chapters 2, 5, Lab manual Pages 173-216.
Lecture and Class Discussion- Biochemistry and metabolism in microorganisms.
Media- Blackboard presentations on Biochemistry of microorganisms and Microbial
Metabolism.
Laboratory Exercises: Lab manual exercises 26-34 Control of Microbial Growth
Learning Unit Topic 3: Understanding Microbial Growth
Competency: 3
Objectives: 3.1, 3.2, 3.3
Activities/ Assignments: Readings- Textbook: Chapter 6 and 7, Lab manual pages 57-69.
Lecture and Class discussions- methods of evaluating microbial growth, important
parameters governing microbial growth, methods of controlling microbial growth.
Media- Blackboard presentation- Microbial Growth I and II.
Laboratory Exercises: Lab manual exercise 8, Use of Aseptic Technique
Learning Unit Topic 4: Bacterial Genetics and Recombinant DNA tecnology
Competency: 4, 7
Objectives: 4.1, 4.2, 4.3, 4.4, 7.1, 7.2, 7.3
Activities/ Assignments: Readings- Textbook: Chapters 8,9 Lab Manual pages 249-255.
Lecture and Class discussions- Introduction genetics and the structure and function of DNA,
Regulation of gene expression, concepts of mutation, recombinant DNA technology,
medical and industrial applications of recombinant DNA technology in microorganisms.
Media- Blackboard presentations on Genetics and Recombinant Biotechnology.
Laboratory Exercises: exercise 41 use of Bergey’s manual
Learning Unit Topic 5: Classification of Microbes
Competency: 5
Objectives: 5.1, 5.2
Activities/ Assignments: Readings- Textbook: Chapters 10, 11, 12, 13 Lab manual pages
217-248. Lecture and Class discussions- Review of Phylogeny and taxonomy, discussion of
prokaryotes, eukaryotes, protozoa, fungi, and viruses.
Media- Blackboard presentations Classification I and II.
Laboratory exercise- 35 morphologic study of bacterium
Learning Unit Topic 6: Principles of Infectious disease, Immunology, and chemotherapy
Competency: 6
Objectives: 6.1, 6.2, 6.3, 6.4
Activities/ Assignments: Reading- Textbook: Chapter 14, 16, 17, 18, 19, and 20.
Lecture and Class discussions- Defining pathology, infection and disease, etiology of
infectious diseases, review of infectious disease transmission, specific and non-specific
host defense mechanisms, and review of common infectious diseases the major
physiologic organ systems, antibiotic chemotherapy.
Media- Blackboard presentations on Infectious diseases, Immunology, Antibiotics.
Laboratory Exercises: Lab manual exercises 37 and 41, Identifying unknowns using
Bergey’s manual
Learning Unit Topic 7: The role of microorganisms in ecological proceese
Competency: 8
Objectives: 8.1, 8.2
Activities/ Assignments: Readings- Textbook: Chapter 27 and Lab Manual pages 285-297.
Lecture and Class discussions- Principles of symbiosis and habitat, soil and aquatic
microbiology, food and disease.
Media- Blackboard presentation- Environmental Microbiology.
Laboratory Exercises: Lab manual exercise 47, Examination of water samples
Course Assessment Information
Description of Course Assessments
(Essays, multiple choice, etc.)
Essay tests, Multiple choice tests, Lab
reports, Library reports
Are exams standardized in your
department?
Yes No
Where can faculty members locate or
access the exams for this course?
Are the exams required by the
department? Yes
NO
Instructors may use test bank that
accompanies the text, in consultation
with other faculty members.