Sarhad J. Agric. Vol.28, No.2, 2012 EFFECT OF SOWING DATES ON THE GROWTH OF TEA SEEDLINGS SAIR SARWAR*, FAYAZ AHMAD*, QAMAR-UZ-ZAMAN*, IJAZ ALI** and ABDUL WAHEED* * ** National Tea Research Institute, Shinkiari, Mansehra – Pakistan. National Agricultural Research Centre, Islamabad – Pakistan. ABSTRACT Study on the effect of different seed sowing dates i.e. November 15, November 30, December 15, December 30 and January 15 on the growth performance of tea seedlings under the agro-ecological condition of Abbaspur, Azad Jammu and Kashmir was conducted at tea nursery Abbaspur district Poonch AJ&K during 2009-10 with the objectives to find out suitable time of tea seed sowing if tea seeds are picked during second week of November. It was found that that maximum survival % age (95.55%), plant height (23.94 cm), root length (21.78 cm) and fresh plant weight (10.83 g) was recorded in plants having sowing date of 15th November 2009, while seeds sown on 30th November produced the highest number of branches plant -1 (1.91) and number of leaves plant-1 (15.92).Survival % age, plant height, number of leaves plant-1 and root length were significantly different for different sowing dates while the differences for number of branches plant-1 and fresh plant weight were found non-significant. It was revealed that early seed sowing dates gave good growth performance as compared to late sowing. Key words: AJ&K, Camellia sinensis L ,growth , seed sowing dates, Tea. Citation: Sarwar, S., F. Ahmad, Q.U. Zaman, I. Ali and A. Waheed. 2012. Effects of sowing dates on the growth of tea seedlings. Sarhad J. Agric. 28(2):179-183 INTRODUCTION Tea (Camellia sinensis. L) is an important commercial crop in many subtropical and tropical areas of the world. Tea, owing to its favorable effects on human health, currently enjoys a great popularity among other beverages worldwide (Sedaghathoor et al. 2009). In Pakistan, tea plantation was first introduced by Pakistan Tea Board in 1958 at village Baffa, District Mansehra in the Khyber Pakhtunkhwa Province. Then PARC established its first Experimental Tea Garden on 2.5 acres at village Daively and with the collaboration of Chinese tea experts surveyed the Northern parts of Khyber Pakhtunkhwa Province on the basis of topography; soil conditions, rainfall and its distribution, temperature and its variation etc. and explored an area of about 64000 ha in Hazara and Malakand Divisions suitable for tea plantation (Pak-China Report, 1982). In order to expand tea cultivation, National Tea Research Institue, Shinkiari, Mansehra surveyed about 4,000 ha area in Poonch, Bagh, Neelum and Jhelum valleys of Azad Jammu and Kashmir and identified the area mostly suitable for tea cultivation. Presently, Ministry of Food and Agriculture, Islamabad launched a project “Commercialization of Tea Production through Public-Private Sector Partnership” for large scale tea plantation by private sector on State land in AJ&K with the technical assistance of National Tea Research Institute. The agroclimatic condition of Distt. Poonch and Bagh of AJ&K were found to be suitable for tea cultivation because of low pH (5.00 – 6.00) and sandy loam characteristics of soils. The total area of AJK is 1,330,220 ha with cultivated area of about 170,787 ha with average farm size of about 1ha and elevation of 1200 to 21062 feet from south to north respectively. The area is mostly hilly and mountainous with numerous valleys and stretches of plains. The area receives rainfall ranging from 1200 mm to1500 mm per annum with and temperatures ranging from -1oC and 35oC. (Information Deptt. AJ&K, 2010). It has been reported by Kathiravetpillia and Kulasegaram (1986) that tea can best be grown on loam and sandy loam soils with pH of about 5.0. Mann (1936) found that tea could be grown on sites with mean monthly minimum temperature of -5oC to 20oC and maximum of 20oC to 30oC in both for tropical and semitropical regions. Richard (1966) reported that the tea production is affected by soil fertility, elevation and weather conditions. Tea may be propagated either by seed or by vegetative means. Seed propagation is a traditional method but it shows a wide range of variation in habit and growth. As tea is a heterozygous plant, there is considerable genetic variability in seedling population (Purseglove, 1984 and Anandappa, 1986). For the best result of tea seed should be planted within few days after picking as it loses its viability rapidly with the passage of time (Eden 1976). Khan et al. (1990) and Waheed et al. (1996) reported that in the tea growing belt of Pakistan seed can best be planted during the month of November just after its picking. According to Wijeratne (2001) the weight of tea shoot is influenced by weather conditions. Tocklai, Tea Research Association (1972) has suggested that the best time to sow seed is early Sair Sarwar et al. Effects of sowing dates on the growth of tea seedlings … 180 November. Seeds may, however, be sown up to the end of December without any appreciable detrimental effect. Seed sown later than this date usually give rise to inferior stunted plants. Sivapalan et al. (1986) reported that tea seed should be sown within shortest possible period after its ripening. Jackson (2005) suggests that Camellia seeds should be sowed immediately after harvest, their germinating capability declines if kept dry. As tea plantation is a new initiative in AJ&K and it is necessary that proper time of seed sowing must be determined for the successful raising of tea nursery under the new climatic conditions, and the present study was conducted by addressing the said issue. MATERIALS AND METHODS This research study was conducted to investigate the effect of different seed sowing dates on the growth of tea seedlings at tea nursery Abbaspur, Poonch, Azad Jammu & Kashmir during 2009 – 2010. Healthy tea seed were collected during second week of November, 2009 from 24 years old tea bushes of Qi-Men variety at NTRI Shinkiari. After removal of fruit coat from harvested fruits, all cracked, infected or damaged seeds were discarded and seeds of uniform size packed in gunny bags and transported to tea nursery Abbaspur. Seed were tested for viability by the Sinker and Floater method (Barua, 1989). The experiment was laid in Randomized Complete Block Design (RCBD) with five (5) treatments replicated three times. Thirty seed (30) per treatment of equal size were used, where as it become total 150 seed used. The polythene tubes have a size of 6 x 18 inch were exercised and filled with sandy loam soil having pH 5.5 collected near by the jungle of pine trees before sowing . Weeds were also eradicate form the tubes and seed was sown at the depth of 2.5 cm on the following dates. Treatments Sowing Dates T1 15th November, 2009 T2 30th November, 2009 T3 15th December, 2009 T4 30th December, 2009 T5 15th January, 2010 All the cultural practices were kept constant. Data regarding survival percentage, plant height, number of branches, number of leaves, root length and fresh plant weight were recorded after 12 months as follows. Percent Survival per Treatment The total number of sapling per treatment which had survived after sowing of seed were counted and percent survival per treatment was calculated by the formula as: Total number of sapling survived Total number of seed planted x 100 Average Plant Height/Plant The shoot length in centimeters was measured by measuring tap of 3 randomly selected sapling of each treatment and average shoot length was calculated after every 30 days. Average Number of Branches per Plant Number of branches were found out by counting the number of branches per plamts in 3 randomly selected sapling of each treatment and average number of branches per cutting was calculated. Average Number of Leaves per Plant Average number of leaves were found out by counting the number of leaves per plant in 3 randomly selected sapling of each treatment and average number of leaves per cutting was calculated. Average Root length per Cutting Root length in centimeters was measured by ordinary measuring tap in 3 randomly selected sapling of each treatment and average shoot length was calculated. 181 Sarhad J. Agric. Vol.28, No.2, 2012 Fresh Plant Weight Average number of leaves (3) were found out by per plant in 3 randomly selected sapling of each treatment and weigh on a digital balance in grams and were calculated on mean basis . Data collected were analyzed statistically by using the analysis of variance techniques with the help of computer software MSTAT-C. Duncan’s New Multiple Range Test was used to determine the differences among the means for different plant growth parameters at 5 percent level of probability. RESUTLS AND DISCUSSION Survival Percentage It is evident from Table I that survival percentage was significantly different among the different sowing dates. The highest survival percentage (95.55%) was recorded in seeds sown on 15th November, 2009 followed by with 86.66% and 68.88% respectively, while the lowest survival % age (51.11%) was recorded in plants having sowing date of 30th December, 2009. Survival percentage decreased gradually with delay of seed sowing time after picking from tea bushes. The increased time period between seed picking and seed sowing might have affected the seed viability, germination and growth of seedlings. Moreover, the early sowing might have provided suitable germination environment as compared to late sowing. These results agree with the findings of Khan et al. (1990) and Waheed et al. (1996) who reported that in the tea growing belt of Pakistan seed can best be planted during the month of November just after its picking. Jackson (2005) has also suggested that Camellia seeds should be sown immediately after harvest, their germinating capability declines if kept dry. Table I Effect of different seed sowing dates on the growth of tea seedlings under the agro-climatic condition of Abbaspur AJK. Treatment T1 15 Nov. T2 30 Nov. T3 15 Dec. T4 30 Dec. T5 15 Jan. LSD (5%) CV% Survival % age 95.6 a 86.7 ab 68.9 bc 51.1 c 60.0 c 21.86 16.03 Plant Height (cm) 23.9 a 19.6 b 18.3 b 17.7 b 17.5 b 2.61 7.16 No. of Branches per plant 1.5 1.9 1.5 1.2 1.1 NS 28.87 No. of Leaves per plant 14.1 ab 15.9 a 12.6 b 11.8 b 11.7 b 2.70 10.83 Root Length (cm) 21.78 a 20.79 a 15.29 b 20.27 a 17.09 ab 4.47 12.49 Fresh Plant weight (g) 10.83 10.75 9.36 9.08 9.31 NS 9.62 Means followed by different letter(s) in same column are significantly different at 5 % level of probability. Plant Height Plant height of tea seedlings was also significantly different for various seed sowing dates Table I. Seed sown on 15th November, 2009 recorded the highest plant height of 23.94 cm followed by 30th Nov, 15th December, 30th December, 2009 and 15th, 2010 with the plant height of 19.60, 18.28, 17.68 and 17.47 cm respectively. Better plant growth results in early sowing coincides with findings of Tocklai, Tea Research Association (1972) who has suggested that the best time to sow seed is early November, extendable up to the end of December without any pronounced negative impact. However, seed sown later than this date usually give rise to inferior stunted plants. Number of Branches per Plant Number of branches per plant ranged from 1.11 (January 15) to 1.91 (November 30) with non-significant differences. It was found that the majority of treatments number of branches plant-1 increased with an increase plant height. The early dates of seed sowing with good growth performance resulted in the production of more branches per plant. The difference in number of branches per plant may also be due to the genetic variability. Purseglove (1984) and Anandappa (1986) have reported that seed propagation is a traditional method but it shows a wide range of variation in habit and growth. As tea is a heterozygous plant, there is considerable genetic variability in seedling population. Number of Leaves per Plant It is evident from Table I that the number of leaves per plant was significantly affected by sowing dates. The highest number of leaves per plant was recorded for 30th November (15.92) and was at par with 15th November sowing while the remaining sowing dates showed non-significant differences. Minimum number of leaves per plant (11.7) was recorded in 15th January sowing date treatment. As the 30th November sowing date gave the highest number of branches per plant, hence it produced the highest number of leaves per plant. Similarly the least number of leaves per plant in the 15th January sowing date may be due to the minimum number of branches and plant height. 182 Sair Sarwar et al. Effects of sowing dates on the growth of tea seedlings … Root Length The differences in root length for treatments November 15, November 30, December 30 and January 15 were at par while these were significantly different from December 15 Table I. The mean values showed that the maximum root length (21.78 cm) was recorded in November 15 followed by November 30, December 30, January 15 and December 15 with root length of 20.79, 20.27, 17.09 and 15.29 cm respectively. A good aerial growth of plant characterized by high number of leaves and hence more carbohydrate synthesis in November 15 and November 30 might have developed a strong and deep rooting system in tea seedlings due to favourabe sowing timings. Fresh Plant Weight The differences for fresh plant weight among the sowing dates were found non-significant. Maximum fresh plant weight (10.83 g) was recorded in November 15 followed by November 30, December 15, January 15 and December 30 having the fresh plant weight of 10.45, 9.36, 9.31 and 9.00 g respectively. As the plants grown in November 15 and November 30 gave the highest plant height, maximum number of branches, number of leaves and root length, therefore the highest fresh plant weight may be due to the maximum biomass production in these treatments. Wijeratne (2001) reported variation in weight of tea shoots as influe. Weather Condition Rainfall particular and other agro-met factor in general are a significant contribution to tea crop as well as other agric. crop production. There is considerable spatial and temporal variability in rainfall & others data characteristic, which affect tea crop production and soil fertility problem. For the adoption of soil & water conservation technique the information of rain fall in particular & other in general is very important. The data presented in the Table II and III describes all the agro-met characteristic in relation with the tea crop production with in the vicinity of Tea Research and Advisory unit of AbassPur AJK Institute Shinkiari, A detail growth parameters of tea seed and its growth behaviors affected on plants during all the study period were also discussed earlier for the efficient utilization of available water for the sustained better survival percentage and its productivity. Table II Statistics of Agro–met at Abbaspur AJK during 2009. Air Temperature Month Humidity % Min Max Jan 6 17 67 February 5 16 78 March 6 21 64 April 9 24 66 May 16 32 55 June 20 31 56 July 20 34 65 August 21 31 73 September 18 29 64 October 12 28 63 November 5 23 60 December 3 21 58 Pan Evaporation 3.221/0.146 2.769/0.125 7.674/0.274 7.719/0.321 14.250/0.527 13.419/0.536 12.489/0.480 7.289/0.291 90.022/0.300 10.515/0.339 4.900/0.168 2.803/0.096 Rainfall (mm/Inches) 72.7 86.36 77.3 140.0 59 87.8 98.3 299.9 208.14 26.9 8.5 Weather condition Clear Cloudy Do Clear Do Clear/sunny Rainey Rainey Hailf Shine Cloudy/rainey Half Shine Clear Source: Agro Met station Tea Res. Adv Unit Abbasspur. Table III Month Jan February March April May June July August September October November December Static’s of Agro–met at Abbaspur AJK during 2010 Air Temperature Humidity % Pan Evaporation Min Max 5 20 52 5.150/0.177 4 14 70 3.550/0.208 10 24 53 8.607/0.358 12 32 57 9.674/0.358 17 31 65 12.868/0.476 18 33 68 14.366/0.495 20 32 77 9.253/0.487 20 31 79 7.452/0.354 18 31 68 8.855/0.368 12 26 57 10.649/0.343 5 28 51 7.042/0.234 -1 22 49 3.621/0.124 Source: Agro Met station Tea Res. Adv Unit Abbasspur. Rainfall (mm/Inches) 28.6 308.8 78.6 43.1 61.8 67.9 409.6 413.7 52.3 9.72 20.3 Weather condition Clear Cloudy/rainey Clear Do Do Cloudy Rainy Rainey Partial cloudy/clear Clear /dry Dry/ clear Cold /cloudy Sarhad J. Agric. Vol.28, No.2, 2012 183 CONCLUSION AND RECOMMENDATIONS On the basis of these findings it was concluded that tea seed sown soon after its picking (second week of November) gave good results as compared to late sowing extended up to 15th January. 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