Des Moines Area Community College
Biology 186 – Microbiology
Spring 2014(Hanlin)
Mon, Tues and Wed 2:30 – 4:30pm
______________________________________________________________________________
Instructor: Mary Beth Hanlin, MS
Books:
1. Microbiology with Diseases by Body System (Bauman) 3rd
Ed.
2. Symbiosis by Mary Beth Hanlin
3. Notes Package – Microbiology Notes
Phone:
433-5062 or 1-800-362-2127 ext. 5062
E-Mail:
mehanlin@dmacc.edu
Office:
Room 222A
Lecture Topics: Tentative schedule attached
* The Microbial World and You
* Microscopy
**TEST 1
* Chemical Principles
* Prokaryotic and Eukaryotic Cells
* Microbial Metabolism
* Microbial Genetics
* Controlling Microbial Growth
**TEST 2
* Microbial Nutrition and Growth
* Recombinant DNA and Biotechnology
* Antimicrobial Drugs
* Characterizing and Classifying Prokaryotes
* Characterizing and Classifying Eukaryotes
* Characterizing and Classifying Viruses, Viroids and Prions
**TEST 3
* Infection, Infectious Diseases and Epidemiology* Innate Immunity
* Adaptive Immunity
* Immunization and Immune Testing
* Immune Disorders
**TEST 4
* Microbial Diseases of the Skin
* Diseases of the Cardiovascular System
* Diseases of the Digestive System
**FINAL
* Diseases of the Nervous System and Eyes
* Diseases of the Respiratory System
* Diseases of the Reproductive and Urinary Systems
Laboratory Topics May Include:
- Use and Care of the Microscope
- Smear Preparation and Simple Staining
- Gram Staining
- Aseptic Technique
- Isolation of Bacteria
-
Examination of Living Microorganisms
Negative and Structural Stains
Microbes in the Environment
Transformation
Preparation of Media
- Microbial Metabolism
- Microbial Growth
- Rapid Identification Methods
- Control of Microbial Growth
Tentative Laboratory Schedule:
Lab
Lab
Lab
Lab
Lab
Lab
Lab
Lab
2
3
21
4
5
6
7,8
10
Lab 17
Lab 12
Lab 11
Lab 16
Lab 20
Lab 18
Microscopy
Examination of Living Organisms
Microbes used in Food Production
Preparation of Smear and Simple Staining
Negative Stain
Gram Stain
Acid Fast, Endospore and Capsule
Environmental Samples with Lab Write up
Culture Media: Making of Media and Pour Plates
Cleaning Products
Pure Culture Techniques in Transfer and Graded on Streak Plating
Serial Dilutions and Pour Plating for Enumeration
Antibiotic Sensitivity
Yogurt/Food Microbiology
Epidemiology/ELISA
Notes for Microbiology Students:
1.
Labs are mandatory for this class and missing is incredibly
difficult to make up. Attendance is essential. There will be NO
lab make-ups.
2.
Please be in class on time because instructions for labs and
exams will be given at that time. A 5 question quiz could happen
any day at the beginning of class.
3.
Academic Dishonesty WILL NOT be tolerated and will result in
an automatic 0 for the test or other assignment. This includes
questions that are assigned from the text and on lab reports. I
expect your own answers, not someone else’s. If there is any
deviation from this, a complaint could be filed and in your records.
4. There will be some in class assignments and homework given
throughout the semester. If you are not in class, it is your
responsibility to get the assignments from others. Some
assignments cannot be made up.
5. Mastering Microbiology: www.masteringmicrobiology.com
This is your homework, quizzes and study helps. The Course ID is
MMBHANLIN08159. Register with the code that I will give you.
6.
Attendance has been documented to have a great affect on
grades. Attendance will be taken every day and at the end of the
semester, attendance may play a part of your grade but only in a
positive way.
7.
Exams: A series of units exams will be given throughout the
semester, the dates will be announced at least one week prior.
THERE WILL BE NO RETAKE EXAMS. IF YOU MISS
AN EXAM, YOU MUST CONTACT ME THE DAY OF
THE EXAM AND MAKE ARRANGEMENTS FOR
TAKING THE EXAM. A PENALTY OF 10% WILL BE
DEDUCTED FROM THE ORIGINAL GRADE FOR
TAKING THE TEST LATE. YOU WILL ONLY HAVE 5
CLASS DAYS IN ORDER TO MAKE UP THE TEST. NO
EXCEPTIONS! No penalty for taking a test early if it is ready.
PRIOR TO TAKING TESTS IN THE CLASSROOM, ALL BOOKS AND BACKPACKS
WILL BE LEFT ON THE FLOOR AS YOU ENTER THE CLASSROOM. CELLS
PHONES WILL BE TURNED OFF AND PLACED IN BACKPACKS!!!!!!!!
8.
Laboratory Reports and assignments: Lab reports and/or
assignments will be given for each lab session. These will be grades and
used in determining you final grade. Lab reports will only be accepted if
you actually participated in the lab. LAB REPORTS ARE DUE 1 WEEK
AFTER THE LAB HAS TAKEN PLACE EXCEPT FOR THE MAJOR LAB WRITE
UP. ANY MATERIALS TURNED IN AFTER THE SCHEDULED DATE WILL NOT
BE ACCEPTED. You will only receive credit for the lab if
you participated in the lab.
9.
No exams will be handed back. Please have your full name,
and class included on everything turned in.
10. There will be NO extra credit work. If you participate and
work hard, you will not need extra credit. That only requires more work
for you and more for me to grade.
11. Evaluation:
Exams .................................................... 50%
In Class Assignments/Homework/Quizzes......……. 15%
Laboratory Reports ..................................… 35%
12. Cell Phone Policy:
NEW EDITION TO CELL PHONE POLICY
ALL CELLS PHONES WILL BE COLLECTED BY ME
BEFORE AND EXAM!!!! NO EXCEPTIONS!!!!
Turn ringer to silent when entering classrooms, labs and
such.
Cells phones with picture taking capabilities will not be
used during class time.
TEXTING WILL NOT BE ALLOWED DURING CLASS. IT IS
RUDE!!!!! IF I CATCH THE TEXTING, I WILL ASK YOU ONE
TIME TO TURN YOUR PHONE OFF.
IF I HAVE TO ASK AGAIN, YOU WILL RECEIVE A 0(ZERO)
FOR ANY WORK DONE THAT DAY.
IF I CATCH YOU TEXTING DURING AN EXAM, YOU WILL
RECEIVE A 0(ZERO) FOR THAT EXAM.
If a call needs to be taken, please quietly remove yourself
to the hall and conduct short, quiet conversations. If
conversations are longer, please move to an area where no
classes are being conducted.
13. Grading Policy:
A
AB+
B
BC+
93% or above
90-92
87-89
83-86
80-82
77-79
C
74-76
CD+
D
D-
70-73
67-69
63-66
60-63
14. Room Policy:
Due to the fact that the room should be clean when you enter, I
should see no garbage in the sinks, in the shelves at your bench, in
drawers. I am not your mom and don’t get paid to clean up after
you. All lab tables should be free of trash, bottles and such before
you leave the room.
Tentative Lecture Schedule:
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15
Week 16
Week 17
Chapt 1
Chapt 2 – Microscope Quiz
Chapt 3
Chapt 4
Test 1 and Chapt 5
Chapt 6
Chapt 7
Chapt 8 and 9
Test 2 and Chapt 10
Spring Break
Chapt 11 and 12
Chapt 13 and Test 3
Chapt 14 and 15
Chapt 16-18
Test 4 and Final project
Final Projects
Finals Monday May 5, 2014 at 2:30pm
It is the policy of DMACC to accommodate students with disabilities. Any student with a documented
disability who requires reasonable accommodation should contact the Special Needs
Coordinator at (515) 964-6850 voice or (515) 964-6810 tty.
Useful websites:
DMACC main page
Course Descriptions
Important Dates
http://www.dmacc.edu
http://www.dmacc.edu/courses/descriptions.htm
http://www.dmacc.edu/academiccal.htm
Des Moines Area Community College
Course Information – EFFECTIVE Aug. 2010
Acronym/Number BIO 186
Title
Credit breakout 4
(credit
Historical Ref BIOL 187/149
3
Microbiology
3
0
0
lecture
lab
practicum
work experience)
PREREQUISITE(S): one semester of any college-level biology
COURSE DESCRIPTION:
A general microbiology course with laboratory designed for the science major. Emphasis is placed
on morphology, physiology, microbial genetics, virology and basic immunology, and applications.
COURSE COMPETENCIES:
During this course, the student will be expected to:
1.
Perform microbiological laboratory procedures
1.1 Understand and comply with safety and health rules of the microbiology laboratory
1.2 Perform all microbiological laboratory procedures according to appropriate safety standards.
1.3 Demonstrate an understanding of the use and care of laboratory microscopes including use of oil
immersion.
1.4 Utilize proper techniques in the isolation and culturing of bacteria.
1.5 Employ and understanding the importance of aseptic technique.
1.6 Perform microbiological staining procedures including the Gram, acid-fast, negative, endospore, flagella,
and capsule stains.
1.7 Differentiate between Gram-negative and Gram-positive bacteria
1.8 Utilize selective, differential, and enrichment media for isolating bacteria.
1.9 Isolate bacteria from the environment.
1.10 Understand and employ the use of biochemical tests in the identification of microbes.
1.11 Use appropriate stains, chemical media, and techniques to identify unknown microbes.
1,12 Perform antibiotic sensitivity assessment of bacteria isolates.
1.13 Demonstrate the use and effectiveness of antiseptics and disinfectants.
1.14 Use serial dilutions to quantify microorganisms.
1.15 Demonstrate and quantify the presence of microorganisms in food and water samples.
1.16 Understand and demonstrate the use of microorganisms in biotechnology and molecular biology
2.
Understand the history and scope of the field of microbiology.
3.
Explain the types, structures and functions of major classes of biological macromolecules.
4.
Demonstrate knowledge of prokaryotic and eukaryotic cell structures and their functions.
5.
Identify the modes of microbial nutrition.
6.
Describe the major cellular metabolic reactions of microbial cells
6.1 Glycolysis
6.2 Fermentation
6.3 Respiration
6.4 Photosynthesis
7.
Discuss microbial growth
Describe influence of environmental factors including temperature, pH, salinity, and oxygen concentration on
growth.
Describe batch and continuous culturing of microorganisms
8.
Explain the processes involved in the flow of genetic information within the microbial cell.
DNA replication
Transcription
Translation
Regulation of gene expression
9.
Describe methods of microbial genetic recombination
Transformation
Tranduction
Conjugation
10. Demonstrate an understanding of the use of microbes in biotechnology and genetic engineering.
11. Explain key viral concepts
11.1 Viral classification
11.2 Viral structure
11.3 Life cycle of viruses
11.4 Pathogenicity of viruses
12. Understand and discuss the diversity of bacterial organisms.
13. Describe concepts relating to infectious disease
13.1 Host defenses
13.2 Virulence
13.3 Pathogenesis of disease
13.4 Epidemiology
13.5 Normal flora
13.6 Identification of disease-producing organisms
13.7 Treatment of infectious diseases
14. Describe the methods for the control of microbial growth.
14.1 Physical methods
14.2 Chemical methods
15. Identify the major classes of antimicrobial agents
15.1 Mechanism of antimicrobial action
15.2 Development and mechanism of antibiotic resistance
16. Demonstrate knowledge of basic immunology
17. Identify the key characteristics of the major groups of eukaryotic microorganisms
17.1 Fungi
17.2 Protozoa
17.3 Algae
18. Discuss and describe the microorganisms associated with disease in the 11 body systems.
18.1 Identify specific organisms with diseases seen in current health watches.
19. Explain major concepts in environmental and industrial microbiology
19.1 Cycling of elements and nutrients in the environment
19.2 Use of microorganisms in industrial processes