Names:
Key
Hour:
Date:
/25 Points
As a group of four, decide who is going to carry out which job (Jobs can be found on page 2).
Follow the instructions below for the three numbers assigned to your group from the sentence
code sheet (Page 3). Be sure to fill out the blanks on both pages 1 and 2 as you go.
1. The DNA stays in the nucleus (which is your assigned lab table). The DNA has the sentence
code sheet out focusing on the # assigned to your lab group. This person’s job is to actually
read out loud the letter code from the code sheet. Remember: each sentence code
represents one side of a DNA molecule. (3 Points)
#
#
#
2. The m-RNA transcribes each codon from the DNA, using base pairing rules, onto the lines
below. This process is transcription; which is the first step of protein synthesis.
(remember to use U instead of T) This step occurs in the nucleus. Example: DNA says “CCC”
you write “GGG”. (3 Points)
#
#
#
code:
code:
code:
3. The m-RNA leaves the nucleus with the transcribed code and goes to the cytoplasm (the
rest of the classroom) to find the ribosome (the student desks). (1 point)
4. The m-RNA and the ribosome tell the t-RNA which anti-codons are needed (cards around
the room). The ribosome writes the anti-codon instructions on the lines below for t-RNA to
take when searching for the appropriate anti-codon and the corresponding amino acid. (3
Points)
#
anti-codon:
#
anti-codon:
#
anti-codon:
5. The t-RNA searches the cell (classroom) for the correct amino acid (word on the back of
the anti-codon card) and begins to create the protein (sentence). This completes the
translation step of protein synthesis. The t-RNA should write the sentence on the lines
below. (3 Points)
#
#
#
protein:
protein:
protein:
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
1
You are responsible for taking notes on each role. You must include information such as
where each person performed their function, what their function was, which step of protein
synthesis they were involved in, which types of nucleotides were involved, how many strands
were involved, etc…
Person #1 = DNA (Name:
)
Where function performed (cytoplasm or nucleus) nucleus
What is their function stores information (read code)
Which step of protein synthesis was involved (transcription or translation)
Which nucleotides were involved (ATCG or AUCG) ATCG
How many strands were involved (one or two)
2
Other Important Info.
Person #2 = m-RNA (
Name:
transcription
)
Where function performed
nucleus & cytoplasm
What is their function transcribes each codon from DNA
Which step of protein synthesis was involved
transcription
Which nucleotides were involved
AUCG
How many strands were involved
1
Other Important Info.
Person #3 = r-RNA (Name:
)
Where function performed
ribosome
tell tRNA which anticodons are needed
What is their function
Which step of protein synthesis was involved
transcription
Which nucleotides were involved
AUCG
How many strands were involved
1
Other Important Info.
Person #4 = t-RNA (Name:
)
Where function performed
cytoplasm
What is their function
find correct amino acid & begins to create protein
Which step of protein synthesis was involved
translation
Which nucleotides were involved
AUCG
How many strands were involved
1
Other Important Info.
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
2
Sentence Code Sheet
Each “sentence code” below represents one side of a DNA strand. You must imagine that
you have “unzipped” a DNA strand and are now focusing on only one side. You will be
transcribing the code into an m-RNA strand and further translating the code using a tRNA molecule with an appropriate amino acid attached. You will, however, be creating a
sentence using words, rather than a protein using amino acids. Good Luck!
1.
ATGAAAAACAAGGTACACATCTAG
2.
ATGAAAAACAATTGCACGTAG
3.
ATGTAAACCACTACATAG
4.
ATGAGAAGTAGGAGAAGCATAATCTAG
5.
ATGATTCAACACATCCAGCCACATTAG
6.
ATGCCCCCGAGAAGCCCTTAG
7.
ATGCGACGCCGGCGTTAG
8.
ATGCTACTCATAGATCTGCTTTAG
9.
ATGTAAAGGGAAGACGAGTAG
10.
ATGCCCCCGGCAGCCGCGTAG
11.
ATGGCTCCGAGAGGAGGCAGAGGGTAG
12.
ATGAAAGGTAAGGTAGTCTAG
13.
ATGAAAGTGAAGGTTTAG
14.
ATGTAAAGGGAATACTATTCATAG
15.
ATGTAATCCTCGTCTCGGCGTTAG
16.
ATGATAGATCTGCTTCCGAGAAGCTAG
17.
ATGCCCCCGGAATGATGCTAG
18.
ATGTGGGTATGTCGGCGTTAG
19.
ATGTTACCGAGATTCTTGTTTTAG
20. ATGTTATCCTCGTGGTTGTTTTAG
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
3
AAC
UAA
CGA
GUA
UAU
CGG
GUG
GAC
AAU
UCG
CCG
UGC
UGG
MOTHER
WE
DRINK
A
THIS
EVERY
BROTHER
DEMENTED
DRESSES
BE
IS
YOU
READ
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
4
CGU
UCU
UUG
GUC
GAA
UUA
AGU
CGC
AUU
CAA
CUG
GCU
AGA
DAY
INFORMED
FOR
DRESS
ALL
DNA
BEATLES
WATER
AN
OLD
ROLL
EDUCATION
THE
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
5
AUC
AAG
GGC
GUU
GGA
CCU
UUC
CUA
GAU
AGC
CUU
GGG
GCC
BAND
WEARS
TO
NOTHING
DOOR
SUBJECT
CODE
I
AND
BEST
MUSIC
FUTURE
MUCH
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
6
ACC
UGU
CCC
ACA
AUA
ACU
CAU
CUC
UUU
GCA
CCA
GGU
AAA
HAVE
LITTLE
BIOLOGY
BREATH
ROCK
DOG
PULLED
LOVE
LIFE
SO
WHEN
FATHER
YOUR
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
7
UAG
AGG
GCG
GAG
CAC
UGA
ACG
CAG
UAC
AUG
UCA
UCC
STOP (.)
ARE
FUN
PUPPIES
RUBBER
AROUND
FUNNY
BREAKS
IN
(START)
TOGETHER
MUST
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
8
PROTEIN “SENTENCE” ANSWERS
1.
Your mother wears a rubber band.
2. Your mother dresses you funny.
3. We have dog breath.
4. The Beatles are the best rock band.
5. An old rubber band breaks when pulled.
6. Biology is the best subject.
7. Drink water every day.
8. I love rock and roll music.
9. We are all demented puppies.
10. Biology is so much fun.
11. Education is the door to the future.
12. Your father wears a dress.
13. Your brother wears nothing.
14. We are all in this together.
15. We must be informed every day.
16. Rock and roll music is the best.
17. Biology is all around you.
18. Read a little every day.
19. DNA is the code for life.
20. DNA must be read for life.
LZHS – Chapter 12 Protein Synthesis Lab
Biology I CP
9