1 BIOLOGY 210: INTRODUCTION TO RESEARCH Professor: Stuart Allison, SMAC B210, x-7185, sallison@knox.edu Assigned Texts: Ambrose, W.H., K.P. Ambrose, D.J. Emlen, and K.L. Bright. 2007. A Handbook of Biological Investigation. 7th Ed. Hunter Textbooks, WinstonSalem, NC. (referred to as HBI) Pechenik, J. A. 2012. A Short Guide to Writing About Biology. 8th Ed. Addison-Wesley Longman, New York. (referred to as WAB) You will also be provided with many pdf copies of articles about maize Course webpage: http://courses.knox.edu/bio210/ Course objectives: 1) develop the basic skills required to do scientific research 2) hone communication skills for presenting research results 3) gain an understanding of how knowledge within the natural sciences (focus on biology) is accumulated 4) further develop skills to work effectively as a member of a group. We will pursue the course objectives by examining the biology and use of corn, more properly known as maize (Zea mays), one of the most important agricultural plants both locally and globally. We will examine the development of maize, its basic ecology and physiology, and the history of human use of maize as a way to better understand biology in general. As we explore the biology of maize, we focus on learning how to do biology, on the process of the science. We will explore: 1) Developing hypotheses – you and your lab team will develop and test independent hypotheses 2) Experimental design – you will be asked to design experiments and studies that will allow you to test the hypotheses you have developed 3) Observations – in order to answer your questions, you must compare your predictions to your observations of biological phenomenon. You will learn to use an array of tools so that you can make your observations in new ways and with more precision than simply using our senses allows us to do. You will almost always be required to quantify your observations. 4) Analysis – What do your observations tell you about your hypothesis? You must perform analysis (usually statistical) that compares your predictions with your observations in order to draw a conclusion about your hypothesis. 5) Communication – you may perform the most important experiment in the world, but if no one knows about it, all your work is for naught. Thus communicating the results of scientific work is a vital part of doing science. You will learn several ways to convey the results of your scientific investigations – oral, visual and written. At the end of the 2 course, you will present your results to the entire biology department so that all can learn what you have been doing and benefit from your work. 6) Ethics – good science, and good policy informed by science, depends on both the reliability of the scientific process and the scientist’s ability to relate scientific investigations to human social concerns. In this course you will become familiar with the standards which govern the practice of science and how scientific results are reported to the public. Many of you will discuss how the results of your research are relevant to social issues. SYLLABUS – FALL TERM 2013 Date Day Topic Sep. 12 Th Intro to Course/Notebook Keeping Sep. 17 Tu Intro to Maize – biology and cultural history Lab: Intro to Greenhouse Sep. 19 Th Scientific method/Experimental Design Lab: Begin planning maize experiment 1 Reading 1-7 (HBI); 149-155 (WAB) Indian Maize – Plants&Society Maize – Economic Botany Photosynthesis - Stern 8-10, 32-38 (HBI) Sep. 24 Tu More on Maize Corn - Gould Lab: Present research proposal, set up experiment Corn - Sokolov Assignment due: Proposal for maize experiment 1 Science behind three sisters Sep. 26 Th Data Analysis (stats 1) 39-48 (HBI); Lab: maize experiment 1 49-68 (WAB) Oct. 1 Oct. 3 Oct. 8 SER Oct. 10 SER Oct. 15 Oct. 17 Oct. 22 Tu Data Analysis (stats 2) cont’d Lab: maize experiment 1 Th Measurement and Data Collection Lab: maize experiment 1 Tu Reading Scientific Papers Lab: Literature Search with Sharon Clayton* And maize experiment 1 Th Work on homework assignment Lab: maize experiment 1 8-15 (HBI) 99-117 (HBI) 21-48 (WAB) Tu Writing Scientific Papers (Methods, Results, 1-20, 49-68, 155Discussion) 203(WAB) Lab: end maize experiment 1 126-140 (HBI) Assignment Due: Homework and Proposal for Maize Exp. 2 Th Scientific Oral Presentations 232-248 (WAB) Tu First Oral Presentations – Maize Exp. 1 Lab: Begin Maize Exp. 2 Assignment Due: Report on Maize Exp. 1 – first draft 3 Oct. 24 Th Data Analysis (stats 3) Lab: Maize Exp. 2 Tu Data Analysis (stats 4) Lab: Maize Exp. 2 Assignment Due: Final Report on Maize Exp. 1 Oct. 31 Th Return to Maize – what have we learned? Lab: Maize Exp. 2 Assignment Due: Homework 49-57 (HBI) Oct. 29 Nov. 5 Nov. 7 Nov. 12 Nov. 14 72-77; 85-98 (HBI) Corn Crisis Food in the future Taking measure of biofuels Wild plants to the rescue Tu Tables, Figures and Graphs Lab: Maize Exp. 2 Assignment – Hand out take-home exam Th Writing Scientific Papers: Intro, Revising, Editing Lab: Maize Exp. 2 Assignment Due: Take Home Exam Tu Scientific Posters Lab: Maize Exp 2 Th Classroom: Open Lab: End Maize Exp. 2 164-191 (WAB) 143-161 (HBI) 203-212;80-122 (WAB) 232-248 (WAB) Nov. 19 Tu Poster Presentations Assignment Due: Report on Maize Exp. 2 – first draft Final Exam Period – Oral Presentations on Maize Exp. 2 Assignment Due: Final report on Maize Exp. 2 * Meet in Caterpillar Computer Lab Class organization. The course objectives will be met through activities in the classroom and the laboratory. 1) Classroom. The classroom sessions will involve lectures, discussions, and group problem solving activities. You should pay close attention to the syllabus and come to class having read and thought about the assigned reading. Lectures, discussions, and group problem solving activities will provide an opportunity to clarify topics covered in the readings - you will get the most out of class if you are prepared to ask questions. You can communicate your questions in class or by e-mail. The group problem solving activities will involve small groups of students (2-3) working together to solve problems and answer questions asked by me. These problems are designed to both clarify what you have learned from the text and classroom discussion and to ask you to go beyond what you have learned by applying your knowledge to new situations. The activities will vary in duration from a couple of minutes to an entire class period. 4 2) Laboratory. The laboratory experience is built around a series of two research projects. These projects give you an opportunity to apply what you have learned in the classroom to explore a variety of biological questions. Over the course of the term, students will be asked to take an increasingly greater role in the design, execution, and reporting of the research projects. You will work in groups of 2-3 students when you conduct your experiments. Course Evaluation: Your final grade in the course will be calculated as follows: Notebooks Exercises/Homework First research proposal Second research proposal Oral Presentation I Oral Presentation II Maize Exp. 1 Report Maize Exp. 2 Report Poster Participation Take Home Exam Total pts. 20 pts. 10 pts. 10 pts. 10 pts. 10 pts. 30 pts. 10 pts. 30 pts. 20 pts. 25 pts. 25 pts. 200 pts. The majority of the graded work will revolve around the research projects. The research project assignments cover a variety of research skills, from writing a research proposal to presenting a scientific talk. The due dates for these assignments are provided on the course syllabus. Details of what each assignment requires will be provided throughout the term. You should note that due dates are subject to change because the nature of experimental biology is that things sometimes take longer than at first anticipated. As mentioned earlier, you will be conducting your research in a group. The actual research, research proposals and oral presentations will be done collaboratively as a group. The lab papers will be written independently. It is imperative that you keep a detailed and up-to-date laboratory notebook. We will start the class with a detailed discussion of what information belongs in the lab notebook and how to present material in the notebook. You should always have your lab notebooks with you in lab and the classroom. At random, unannounced times during the term, I will collect the lab notebooks and grade them. You will also need to turn in your lab notebook for a final grade at the end of the term. The discussions, group problem solving activities, and group participation in research means that your contribution is vital to the success of the class. As a result your class participation will be graded. The participation grade will not assess how “clever” or “talkative” students are, but will instead assess how well each student has contributed to the class. In making this assessment I will ask myself the following questions: • To what degree was the participant ready for each day’s activity? Had the participant read the assigned readings critically and come to class prepared to discuss the material? 5 • Was the participant willing to ask questions about the material being discussed? Did the participant exhibit “academic honesty” - asking questions when things were unclear to them and admitting when they didn’t understand something? • Did the participant try to answer the questions of others in a helpful way? • Was the participant willing to be wrong sometimes? Did the participant ask and answer questions and make observations that suggested that s/he was stretching him/herself and not overly afraid of saying something that might be wrong? • Did the participation of this person enhance the understanding of the group? • Was this person considerate in the way they participated in the group or did they dominate the conversation too much, making it difficult for others to speak? • Did this person exhibit a positive attitude? Did they show an enthusiasm to learn and participate or did they do only what they had to? • Was this person a valued member of the group, contributing to the best of her/his abilities? • Did the participant put in her/his fair share of the work in completing the assignments? The mid-term exam will be an open note, open book, take-home exam. It will test your under-standing of the material and your ability to apply your understanding in solving novel problems, not how much you have memorized. While the exam is in progress you may not talk to anyone (except the instructor) about ANY aspect of the exam (including whether you are finding it easy or hard, long or short etc.). Absence policy. Students are expected to attend all classes. If you must miss a class for religious or athletic or others, please let me know in advance. If you are ill, please have a note sent to me from campus health verifying that you were ill. Grading scale: 90 - 100% - A 80-89% - B 70-79% - C 60-69% - D < 60% - F