Name: ___________ Plasma Membrane and Cellular Transport Remedial Assignment (Just for the record … the preferred method of completing and turning in this assignment is to “save as” to your own directory, answer (in another colour) on computer and then send me the .doc via email (rstevenson@papcs.com) Of course, you could always print it up and turn in but hey, a few less trees murdered seems better to me. If any of this is confusing see me about it) PLASMA MEMBRANE & CELLULAR TRANSPORT A semipermeable membrane that regulates interactions of the cell and its surroundings covers each cell. Functions include separating the cell from its outside environment, controlling which molecules enter and leave the cell, and recognizing chemical signals. Passive and active transport contribute to homeostasis. The processes of diffusion and active transport are important in the movement of materials in and out of cells. Structure of the Plasma Membrane Structure of the Plasma Membrane Now load the CyberEd Biology Course Title: The Plasma Membrane and Cellular Transport. View scenes 1 –5 and complete the exercise below. Multimedia Presentation View the structural components and their role in the plasma membrane. Scene 1 1. Why do you think it is important to have a membrane surrounding each of our trillions of cells? Expect varying answers, but the idea is have the students understand the plasma membrane separates the cells from their environment and each other while also regulating the material within each cell. Scene 2 - 3 2. What are two distinctive physical features of phospholipids? Heads are polar (water soluble, hydrophilic) and (water insoluble, hydrophobic). 3. Describe the conformation of the phospholipid bilayer of the plasma membrane. What abundant fluid leads to this conformation? Because the phospholipids heads water. Scene 4 4. How does the plasma membrane demonstrate its fluid nature of the Fluid Mosaic Model? What molecule in the plasma membrane directly affects the membrane’s fluidity? The phospholipids of 5. How does the plasma membrane demonstrate its mosaic nature of the Fluid Mosaic Model? Protein molecules are embedded within the phospholipid Scene 5 6. What is the function of the glycoproteins and glycolipids of the plasma membrane? serve as cellular identifiers or signatures. They help the cell recognize friend and foe. 7. Use the illustration to identify the following components of the plasma membrane: cholesterol, glycoprotein, phospholipids, membrane protein, and glycolipid. A. Glycoprotein B. C. Membrane protein D. Cholesterol E. Structures of the Plasma Membrane Please load the CyberEd Biology Course Title: The Plasma Membrane and Cellular Transport. Complete Interactive Lessons # 1. Practice identifying the structures of the plasma membrane. Interactive Lesson Cellular Transport: Diffusion and Osmosis Cellular Transport: Diffusion and Osmosis Now load the CyberEd Biology Course Title: The Plasma Membrane and Cellular Transport. View scenes 6 - 19 and complete the exercise below. Multimedia Presentation Learn about the movement of molecules along a concentration gradient in solutions. Scene 6 1. Define cellular homeostasis. How does the plasma membrane contribute to cellular homeostasis? Cellular homeostasis is the maintenance of a constant environment within a cell, in which the plasma membrane regulates the passage of molecules across it. Scene 8 2. Describe the movement Scottish scientist Robert Brown observed in the suspended pollen grains. What causes the pollen to move in such a way and what is this motion now known as? Brown observed the constant jittery movements of pollen grains suspended in water. The motion of pollen grains was a result of the random motion and collision of molecules, now known as Brownian motion. Scene 9 3. Define diffusion. Diffusion is the movement of particles from a region of high concentration to a region of lower concentration. Scene 10 4. What is the concentration gradient? The concentration gradient is a difference in concentration between a region of higher concentration and region of lower concentration. 5. Use the graphics (A and B) to answer the following questions on diffusion. 5a. The moving food coloring particles in Glass A demonstrate what molecular process? Diffusion 5b. What conditions must exist in Glass A for this movement to occur? There must be a concentration gradient, a difference in concentration between the food coloring and the water. 5c. In Glass B, what is the concentration difference between food coloring and water? None 5d. When the concentration of food coloring and water in Glass B reach this balance, what is this state called? Dynamic equilibrium Scene 11 6. The cell continually produces carbon dioxide as a by-product of cellular respiration. How does the cell keep carbon dioxide from building up in the cytoplasm and poisoning the cell? Carbon dioxide diffuses across the plasma membrane into the where its concentration is lower than in the cell. Scene 12 7. What distinguishes osmosis from diffusion? Osmosis is the diffusion of water, whereas diffusion can be applied to any molecule. Scenes 13 – 14 8. If the concentration of protein in one solution (Solution A) is greater than a second solution (Solution B), in which solution is the concentration of water greater? The second solution (Solution B) 9. Use the illustration to answer the following questions about osmosis. 9a. In which direction will water move through the membrane? If the proteins could diffuse, in which direction would they move? Water will move from B to A. Proteins would diffuse from A to B, if they could. 9b. Osmotic pressure is the pressure exerted by water on its container as it moves by osmosis into a region of lower water concentration. What do you think might happen if a pressure equivalent to osmotic pressure was applied to side A? The water would not move from side B t Scene 15 – 19 10. Fill in the summary tables for each type of solutions. 10a. What is an isotonic solution? The concentration of dissolved substances is equal inside the cytoplasm and in the extracellular fluid. Inside the cell Concentration of dissolved substances Equal Concentration of water Equal Membrane Isotonic Solution Outside the cell Equal Equal What happens to an animal cells? Dynamic Equilibrium What happens to a plant cell? Dynamic Equilibrium 10b. What is a hypotonic solution? The concentration of dissolved substances is less in fluid than in the cytoplasm. Inside the cell Concentration of dissolved substances Greater Concentration of water What happens to an animal cells? Less Membrane Hypotonic Solution Outside the cell Less Greater Water moves into the cell, leading to swelling and bursting Water moves into the central vacuole, What happens to a plant cell? 10c. What is a hypertonic solution? The concentration of dissolved substances is fluid than in the cytoplasm. Inside the cell Concentration of dissolved substances Less Concentration of water What happens to an animal cells? What happens to a plant cell? Greater Membrane Hypertonic Solution Outside the cell Greater Less Water moves out of the cell, leading to shrinkage Water moves out of the cell, pressure, & leading to Osmosis Please load the CyberEd Biology Course Title: The Plasma Membrane and Cellular Transport. Complete Interactive Lessons # 3. A review of osmosis in the cell. Interactive Lesson Cellular Transport: Passive and Active Transport Cellular Transport: Passive and Active Transport Now load the CyberEd Biology Course Title: The Plasma Membrane and Cellular Transport. View scenes 20 - 29 and complete the exercise below. Multimedia Presentation Examine passive and active transport as two mechanisms helping to maintain cellular homeostasis. Scene 20 1. In passive transport, what determines the direction of movement of small particles? The direction of movement of particles in passive transport is determined by the concentration gradient (diffusion) between the cytoplasm fluid. Scenes 21 – 22 2. Why do the molecules in facilitated diffusion need help moving across the plasma membrane? Likely, the molecules are too large to pass unaided through the plasma membrane with the concentration gradient; they need to pass through special transport proteins. 3. Does facilitated diffusion require the cell to expend energy to move substances? Why or why not? No, the molecules move by simple diffusion, they just require a concentration gradient and special transport proteins to help them pass through the plasma membrane. Scene 23 4. Fill in the table, identifying similarities and differences between passive transport and active transport. What drives the movement of the molecules across the plasma membrane? Do the molecules move with or against the concentration gradient? What size are the molecules that are moved across the plasma membrane? Passive Transport Active Transport Concentration Gradient ATP With the gradient Usually against the gradient, but can be with it Small for simple diffusion or osmosis, larger for facilitated diffusion Any size Scene 24 5. Recall that a cell needs to maintain homeostasis to perform its functions. If a cell has a higher concentration of substances in the cytoplasm, which direction will water move? How can a cell continue to maintain homeostasis if the concentration of water fluctuates? Water will move into the cell. Active transport can be used to move water and other molecules into the cell or out as needed. Scene 25 6. Define endocytosis. Scene 26 7. Describe the steps in endocytosis from the following series of illustrations. 7a. Describe this step of endocytosis. The plasma membrane begins engulfing particles from 7b. Describe this step of endocytosis. The plasma membrane completely surrounds the particles and pinches off into the cytoplasm to form a vesicle, a plasma membrane enclosed sac with the particles. 7c. Describe the final step of endocytosis. The vesicle of digestive enzymes. The enzymes break down the particles engulfed in the vesicles. Scene 27 8. Sometimes cells receive messages from larger molecules (like hormones). How might these larger messenger molecules cross the plasma membrane? How is this different from simple endocytosis? Scene 28 9. Define exocytosis. What type of transport is exocytosis? Why might it be important for the cell to carry out exocytosis? The cell needs to remove waste products from the cytoplasm, but because they may be too large to diffuse, or the concentration gradient is against the transport of the waste this function. Scene 29 10. Fill in the summary table for particle transport across the plasma membrane. Powered by Examples Passive Transport Concentration Gradient Active Transport ATP Diffusion Osmosis Facilitated Diffusion Facilitated Transport In which direction do molecules cross the plasma membrane? Both Both Both Both Into the cell Out of the cell Active Transport Please load the CyberEd Biology Course Title: The Plasma Membrane and Cellular Transport. Complete Interactive Lessons # 2. Examples of active transport in cells. Interactive Lesson Active Transport Please load the CyberEd Biology Course Title: The Plasma Membrane and Cellular Transport. Complete Interactive Lessons # 4. Interactive Lesson A review of the major mechanisms to move molecules across the plasma membrane. Comprehensive Exam: The Plasma Membrane and Cellular Transport Please take the computerized random CD-Rom exam provided by your teacher. Test