Prestained SDS molecular weight standard mixture AND Dalton mark VII-Lfor SDS Gel electrophoresis,both made up in sample buffer and urea(this may or may not be added,if added note additionasl risk phrases for urea.
Prestained SDS molecular weight standard mixture.
Urea
Hazardous Substances Policy - Assessment
8M Urea
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr. T. W. Young
Assessor
C.Sampayo
Assessment Number*
1793
Date of Assessment
11-04-2001
Signature
Signature
-otes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
Dr. T. W. Young, A. Wadeson, Ph.D. students
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Prestained SDS
molecular weight
standard mixture AND
Dalton mark VII-Lfor SDS
Gel electrophoresis,both
made up in sample buffer
and urea(this may or may
not be added,if added
note additionasl risk
phrases for urea.
Prestained SDS
molecular weight
standard mixture.
Urea
Laboratory G6
The use of Sigma molecular weight markers for SDS PAGE.
AMOUNT
max/stored
1.5ml
max.
1vial of
dry
material.
Attach copies of data sheet(s)
HAZARD
RISK PHRASES
Toxic
BIOHAZARD
Potentially
infectious
R23/24/25-Toxic by
inhalation ,in contact with
skin and if swallowed
R42-May cause sensitation
by inhalation. Sigma
recommend,treating as
capable of transmitting
infectious agents.May be
Harmful by
Inhalation,ingestion or skin
absorption,toxicological
properties not thoroughly
investigated.
If Urea
addedthen
include,
Harmful
R40-Possible risk of
irreversible effects.
R36/37/38-Irritating to eyes
respiratory system and
skin.
BIOHAZARD
Potentially
infectious
No risk phrases given by
Sigma,treat as capable of
transmitting infectious
agents.May be Harmful by
Inhalation,ingestion or skin
absorption,toxicological
properties not thoroughly
investigated.
R40-Possible risk of
irreversible effects.
R36/37/38-Irritating to eyes
respiratory system and
skin.
Harmful
HAZDAT NO***
BIOSCIENCESNO***
8M Urea
Harmful
Dalton mark VII-Lfor SDS
Gel electrophoresis
3.5mg
Potentially
HARMFUL
2-Mercaptoethanol
100mls
Toxic
Sample buffer(Sigma
product S3401)Solution
contains,4%SDS,20%Gly
cerol,10%2Mercaptoethanol,0.004%
Bromophenol
Blue,0.125M Tris HCl,pH
approx 6.8.
1ml vials
Toxic
5
R40-Possible risk of
irreversible effects.
R36/37/38-Irritating to eyes
respiratory system and
skin.
May be Harmful by
Inhalation,ingestion or skin
absorption,toxicological
properties not thoroughly
investigated.
R24-Toxic in contact with
skin.
R20/22-Harmful by
inhalation and if
swallowed.
R41-Risk of serious
damage to eyes.
R37/38-Irritating to
respiratory system and
skin.
R23/24/25-Toxic by
inhalation ,in contact with
skin and if swallowed
R42-May cause sensitation
by inhalation.
INTENDED USE**
Give brief details and attach protocol/instructions
To be used as unstained and prestained markers for molecular
Weight identification on SDS PAGE gels.
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
From the stock reagents there is a risk of skin and eye contact and risk of inhalation of dust and vapours which
may also cause sensitisation in the case of sample buffer.There is a risk of skin and eye contact and inhalation of
Aerosols from the solutions when handling and pipetting.There is also an additional BIOHAZARD risk with the
Molecular weight markers,they have the potential to transmit infectious agents.
After sample denaturation by heating in Sample buffer the Biohazard should be absent but up until this stage all
materials coming into contact with the potentially infectious material should be autoclaved.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The risks associated with all the compounds and reagents are significant.
Greater precautions than Good Chemical Practice should be followed ,the fume cupboard should be used
especially when 2-mercaptoethanol is in use. All equipment , which contain potentially infectious agents, such as
pipettes and assay waste should be autoclaved.
NOTE- After sample denaturation by heating in Sample buffer the Biohazard should be absent but up until this
stage all materials coming into contact with the potentially infectious material should be autoclaved.
8
CONTROL MEASURES
Additional to Good Chemical Practice
In addition to Good Chemical Practice,care should be taken to minimise inhalation of substances and aerosols
especially where they contain potentially infectious agents and 2-Mercaptoethanol.Appropriate face masks should
be used . The fume cupboard should be used when and if 2-Mercaptoethanol is added to the sample buffer.
Waste containing molecular weight Markers which contain potentially infectious agents should be autoclaved, this
includes such items as pipette tips, and contaminated equipment and glassware.
NOTE- After sample denaturation by heating in Sample buffer the Biohazard should be absent but up until this
stage all materials coming into contact with the potentially infectious material should be autoclaved.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
General Laboratory Practice
Only experienced laboratory personnel should be using these compounds and reagents.
10
MONITORING
Performance of control measures,
Personal exposure
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Small quantities of reagents and solutions should be disposed of to the non-halogenated waste bottle.
Chemicals can be disposed of according to the hazardous substances policy of the University.
Autoclaved waste should be drained of any excess liquid, bagged and disposed of to the yellow bag laboratory
waste bin.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
April 2006. 0r any change in protocol or amounts used.
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Only adequately trained and experienced personnel should deal with emergency spills.
Solutions involved are small volumes .A chemical spill kit can be used.
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
Only adequately trained and experienced personnel should deal with emergency spills.
Gloves and eye glasses should be worn to prevent contact with spills of the above solutions.
Respiratory protection should be worn in the event of a major spill of 2-mercaproethanol. See attached Unicoshh
Datasheet number 511 for further details.
TO RENDER SITE OF EMERGENCY SAFE
Wash spill site with soap and water and dry.
CONTACT
Clean-up/decontamination
DR YOUNG/A.WADESON.
PHONE
3523
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.