Biology 30 Module 1 Cells and Biochemical Actions: Foundations of Life Lesson 1 The Cell Copyright: Ministry of Education, Saskatchewan May be reproduced for educational purposes Biology 30 1 Lesson 1 Biology 30 2 Lesson 1 Lesson 1 The Cell Directions for completing the lesson: Text References for Suggested Reading: BSCS Biology Chapter 5, Pages 99-107 Section 5.1 - 5.5; Appendix 2 P. 670 OR Nelson: Biology Chapter 1, pages 20-39 Study the instructional portion of the lesson Review the vocabulary list Do Assignment 1 Biology 30 3 Lesson 1 Vocabulary cell theory mitochondria cell wall nuclear membrane centrifuging nuclear pore centrioles nucleolus chromatin nucleoplasm cytoplasm nucleus cytoplasmic streaming organelles cytosol phase-contrast microscope dark-field microscope phospholipid molecule differentially permeable plastid endoplasmic reticulum prokaryotes/prokaryotic eukaryotes/eukaryotic protoplasm fluid mosaic model ribosomes golgi complex semi-permeable lysosomes selectively permeable microtoming vacuole microtubules Biology 30 4 Lesson 1 The Cell Introduction From the moment we are born, our various senses are assaulted with a multitude of different kinds of stimuli or sensations. We become accustomed to many of them and, at any particular time, we are consciously aware of or pay attention to only a small fraction of them. The types of responses we make or the kinds of reactions which we may have are often the result of learning processes which are continually taking place. Learning builds up the knowledge which we have about our Photo by Andreas Praefcke - GNU environments. Some of that knowledge is put to practical everyday use, while a large amount is probably stored in our minds as part of many general understandings about that which is taking place around us. We live in an age of science characterized by rapid gains in knowledge. Expansion in general and scientific information has been coming at ever increasing rates with advances leading to more advances. Present estimates seem to indicate that our scientific knowledge is doubling every five to ten years. This is in sharp contrast to the rather slow developments or accumulations which had been occurring up to the early 1900's. Many of the technological advances (particularly in the computer fields) are often outdated before even becoming fully available to the public. Rapid advances have also occurred in such varied fields as travel, communications, satellites, food preparations and medical accomplishments. Biology 30 5 Lesson 1 The accumulation of new knowledge and the development of more advanced scientific techniques and instruments have opened up areas of interest which had not previously been studied or thought of before. However, many kinds of studies have also been receiving attention for hundreds of years. Attention in these areas have been maintained as new scientific developments enabled us to probe for information and answers previously unavailable to us. Despite the continued uncovering of new information, many "old" questions remain unanswered and newly uncovered information has often led to new questions. One such broad area of interest which had intrigued our ancestors and continues to do the same to us revolves around the characteristics of life and all those actions involved in sustaining life. Studies of life and body functions eventually lead to cellular studies, or cytology. Cells are the smallest units of organisms. Any actions necessary for maintaining the life of an entire organism must occur in cells first. This first lesson will examine some of the kinds of cell studies which have taken place and the information which has been uncovered about cell structures up to this time. Biology 30 6 Lesson 1 After completing this lesson you should be able to: • identify some individuals who made contributions in the early beginnings of cell studies. • have an understanding of the different kinds of microscopes and their general role in cytology. • mention some other developments or techniques which have helped in cell studies. • summarize the major points of the Cell Theory. • recall some of the major cell structures or organelles and their apparent values to cells. • state some major differences between plant and animal cells. • explain the various relationships within an organism and the cellular arrangements which make up an efficient, functioning organism. Biology 30 7 Lesson 1 Early Cell Studies Can you imagine not knowing that cells exist? Looking at your hand, you would have thought that it was just a solid mass. A person with 20/20 vision has a resolving power down to 0.1 mm, that is if two lines were less than 0.1 mm apart they would appear as a single line, or a dot with that diameter may just barely be visible to some people. The human egg is approximately this size, but most of a human body's 50 to 70 trillion cells are much smaller than this. Even the longest nerve cell, which may extend a little over one meter (from the lower back and into the foot), has a diameter too small to be seen without the aid of a microscope. It is worthwhile to take a step back in time to see how the study of cells began with the discovery of magnifying lenses and how this leads into the development of the microscope. Microscopy The credit for developing the first magnifiers appears to belong to two Dutch brothers by the name of Janssen. Their development of magnifying lenses came in the 1500's. Whether he had some knowledge of the work of the Janssen brothers or not, Antony van Leeuwenhoek eventually came to be regarded as being mainly responsible for opening up the field of microscopy. He was from Holland. In the late 1600's and early 1700's, he produced many hundreds of single lenses or simple microscopes to study many different things. Somewhat of a jealous person in that Leeuwenhoek never permitted anyone to freely handle his microscopes or know of his exact techniques in producing lenses, he nevertheless kept careful records of the discoveries and observations of things that he examined. These he passed along to a scientific organization, the Royal Society of England. Leeuwenhoek was probably the first individual to observe single-celled microorganisms such as yeasts, bacteria, blood and sperm cells, and some of the common protozoans found in water. A considerable length of time (about 150 years) went by before microorganisms began to be observed by anyone else. Biology 30 8 Lesson 1 Just prior to Leeuwenhoek's discoveries, English scientist Robert Hooke issued a publication (in 1665) recording his observations and descriptions of cells. Hooke used a compound microscope, which made use of two sets of separate lenses. Hooke Microscope Hooke was actually the first to observe groups of cells. These initial observations were really of dead cork cells with just their outer walls remaining. Observations of living cells from other organisms began to be made by Hooke as well as other individuals. However, at the time, none of these really established the relationship or the importance of cells to an entire organism. Biology 30 9 Lesson 1 The initial discoveries and observations of microorganisms and cells were made possible by the use of light microscopes. Leeuwenhoek's single lens magnifiers—comparable to a simple hand-held magnifier of today—and the double-lens or compound microscopes make use of light which passes through or is reflected off an object being observed. Handheld devices can enlarge up to 20 times actual size The Compound Light Microscope Leeuwenhoek's fine work is shown by his ability to produce one simple microscope which magnified approximately 270 times. Monocular compound light microscopes found in school laboratories today vary in magnification from 100 to 400 times and upward. The double magnification systems of some of the better compound light microscopes can approach 2000 times but not much more, as wavelengths of light are such that details cannot be resolved much beyond this (much as our eyes cannot resolve details smaller than 0.1 mm) and the image will blur. Additional magnification will not improve the ability to see detail clearly, because of the physical characteristics of light. Biology 30 10 Lesson 1 The development of electron microscopes after the 1930's increased the ability to magnify dramatically. Instead of using light, electrons are beamed through an object and onto a photographic plate – since electrons are invisible to the eye. The differences in the numbers of electrons passing through or being absorbed by various parts of the object are responsible for creating the image. Between the object and the photographic plate are magnetic fields which act in the same manner as lenses to light by spreading or condensing electrons, to achieve high magnifications. Electron microscope magnifications are usually hundreds of thousands times and can even be over a million times. Photo by tz1_1zt Aids used to study cells 1. Microscopes can magnify and resolve images for the most part, but differences between certain observed parts are so slight that they cannot be clearly distinguished from each other. Different types of microscopes have been developed for this purpose: Dark-field microscopes bend only those light rays passing through a slide or an object, which makes the image appear bright against a dark background. This shows up cell structures that are invisible with a light microscope. Phase-contrast microscopes bend the light rays passing through an object in such a way as to make nearby parts stand out from each other. This type of microscope makes it possible to study very small structures and events occurring in living cells. Electron microscopes show cell structures at very large magnifications. Two types used are: o transmission electron microscopes for enlarging cell organelles o the scanning electron microscope for more detailed observations. Biology 30 11 Lesson 1 2. Special fixatives for killing cells and many different kinds of stains which are absorbed differently have also been developed for distinguishing between cell parts. Hundreds of stains and staining techniques are presently being used. 3. Microtoming, or the slicing of objects into very thin sections, provides transparent slices for examination with an electron microscope. 4. The grinding up and centrifuging of objects to separate different substances has undergone continual refinements to aid in cellular studies. The Cell Theory For many years after cells were first observed, their importance or significance to organisms' bodies was not realized. They were simply regarded as "being there". In 1809, French naturalist Jean Lamarck made a significant interpretation and generalization when he noted that every living body seemed to consist of masses of cells, each containing moving fluids. It was not until 1839 that two German biologists, Schleiden and Schwann, independently stated their ideas which formed an important beginning to the Cell Theory. Their generalization was: All organisms are made up of cells, whether unicellular (single cell) or multicellular (many cells). That is, cells are the basic structural units making up all bodies. The second and third important generalizations making up the Cell Theory came from the later works of German pathologist Rudolf Virchow. The statements he added were: All processes common to living organisms occur in the individual cells meaning that individual cells carry out basic functions necessary to maintain the life of an entire organism (such as respiration, excretion, ingestion and others). All new cells arise from existing cells. More recently scientists have added a fourth statement to the Cell Theory. It states: Cells contain hereditary material, which ensures the passing of specific characteristics from parent to daughter cells. Biology 30 12 Lesson 1 The statements making up the Cell Theory can be applied as characteristics of living organisms. One or two possible exceptions exist: Viruses are unique in a number of ways. They do not show any criteria for life. They appear to show life only when inside a living host. Rather than carrying out their own life-sustaining functions such as respiration and growth, they re-direct those of their hosts' to satisfy their own requirements to produce more viral matter. Some protozoans, algae and fungi are also difficult to fit into the Cell Theory. These organisms may contain structures unusual to most other cells. In addition, it is difficult to determine whether some of their masses are single-celled or multi-celled. Cell Structure and Function Developments in microscopy, staining and other techniques related to cellular examinations, enabled scientists to begin identifying individual cell parts. Experiments and various kinds of testing helped to determine what the function of some of these parts were. The functions of some distinct structures are still not fully known. The nature or form of these structures and some of their behaviors have led to scientists stating what they think they do in a cell, but without any definite certainties. There is no "normal" or "typical" cell which can be used as a common representative for all organisms. Differences exist in cell sizes, shapes, colors and in the kinds of structures found within each. These differences exist not only between different kinds of organisms but also within individual organisms. If one was to combine all the possible features of all cells into one, it may be possible to produce a general or "master" cell which could be used as a study model. It should be emphasized again that such a cell would not really exist under natural conditions. The term protoplasm was applied (by Hugo von Mohl in the 1800's) to all the living material within cells. Most cells have a rounded body or nucleus within them so that the living matter could be further distinguished by labelling all protoplasm within the nucleus as nucleoplasm and the remainder outside the nucleus as cytoplasm. (Again, remember that there are exceptions to the general form as with human red blood cells which have no nuclei at maturity and some muscle cells have several.) Within the protoplasm, particularly in the cytoplasm, are small, distinct bodies called organelles. Collectively, these carry out various functions related to sustaining life such as energy release or storage, growth and repair, secretion and other actions. Biology 30 13 Lesson 1 There are two major types of cells. 1. Cells that have a distinct, membrane-enclosed nucleus that contains the cell's DNA are called eukaryotes or are eukaryotic. In addition to having nuclei, some of these cells have photosynthetic membranes enclosed in distinct bodies or organelles (called chloroplasts). There are other membrane-enclosed organelles so that, in general, the cytoplasm of a eukaryote shows a fair degree of specialization. 2. Prokaryotes are noted by the lack of distinct nuclear bodies. The absence of nuclei does not mean an absence of nuclear material. The nuclear matter is scattered throughout the cytoplasm. Any photosynthetic membranes present are also freely floating within the cytoplasm. The absence of membrane-enclosed organelles and general lack of specialization is a common feature of prokaryotes. A Prokaryotic Cell A Eukaryotic Cell (example of a bacteria cell) (example of an animal cell) Chloroplast Nucleoid (circular DNA) Biology 30 Cell Wall Nucleolis Cell Membrane 14 Nucleus Lesson 1 With some noticeable differences in form and the presence or absence of some structures, the following graphics illustrate "typical" plant and animal cells. Biology 30 15 Lesson 1 Cell Wall The cell wall is found in plants and in some monerans and fungi. Rather thick, its largely cellulose nature forms a rigid but porous framework which appears to provide both support and protection for plant cells. Its rigidity generally results in plant cells showing greater regularity or uniformity in their shapes, as compared to animal cells. Cell (or plasma) Membrane Surrounding both plant and animal cells is a thin, flexible and living plasma membrane. In plant cells it is often pressing against the inside of the cell wall. The membrane is fluid and thus, flexible. It consists of two phospholipid layers called a bilayer. o the outer part or ‘head’ of the phosopholipid molecule is attractive to water o the inner chain or ‘tail’ is not. This characteristic enables the membrane to maintain a moist exterior while offering some barrier to movements of molecules through it. These phospholipid molecules move and float across the membrane. Different protein molecules are embedded in and extend right through both layers of the membrane forming a pattern or mosaic as shown in the diagram below. These protein molecules drift across the membrane as well. This model of the cell membrane structure is referred to as the fluid mosaic model. The Fluid Mosaic Model of the Cell Membrane Membranes not only surround or enclose entire cells, but also form the outer boundaries of various organelles within the cytoplasm. The actual kinds of phospholipids or proteins making up membranes or the ways in which these molecules are actually arranged have effects on what roles membranes have. Most of the membranes protectively enclose not only the contents of entire cells or those of distinct organelles but also seem to control the movements of substances through them. phosphate head phospholipid layers (bi-layer) lipid tail protein molecules Biology 30 16 Lesson 1 The selective nature of such membranes to various substances means that they are semi-permeable or differentially permeable. Another term, selectively permeable can also be used. The balancing of the concentrations of substances on either side of the membranes can be crucial to the proper functioning of cells or their organelles. (Some of the movements through cell membranes will receive further attention in the next lesson.) In addition to regulating movements, some membranes may form partnerships with particular hormones during the synthesis of larger molecules. Cytoplasm The cytoplasm contains all the living cell material that extends from the cell membrane to the nuclear membrane. The cytoplasm contains Cytosol Organelles Cytosol The cytosol is the gelatinlike portion of the cytoplasm that bathes the organelles. The cytosol contains a high percentage of water. The rest is made up of amino acids, lipids, carbohydrates, enzymes, salts, minerals and other elements. All of these are necessary for the various metabolic processes that are required to keep the cell alive. These processes are kept separate or are prevented from interfering with each other by being contained in smaller structures scattered throughout cytosol called organelles. Organelles nuclear membrane 1. Nucleus nucleolus Most cells contain rounded structures which are commonly in the center in many animals or off to one side, as in many plants. These nuclei appear to be the "control centers" for many cell activities and are of particular importance for cell reproduction and growth. Each nucleus is surrounded by a membrane much like the plasma membrane described earlier, except that the nuclear membrane is two-layered or double. Pores in the membrane probably allow certain substances, which could include "messengers", to travel chromatin between the cytoplasm and nucleoplasm. Nuclear pore nucleoplasm Varying numbers of dense bodies called nucleoli may be present. In plants or animals where they do appear, the actual number is specific for a species although not all may be visible at any one particular time. (Nucleoli Biology 30 17 Lesson 1 commonly disappear just before cell division occurs.) A nucleolus appears to consist largely of proteins and nucleic acids (RNA or ribonucleic acid) which direct or take part in protein synthesis carried on within a particular cell. In other words, a particular kind of human cell will keep reproducing its own kind of protein or, when the cell reproduces, new human cells of the same type will be formed. All eukaryotic cells (those with distinct nuclei) have thread-like structures of protein and nucleic acids (mainly DNA or deoxyribonucleic acids) scattered through the nucleoplasm. This is chromatin and shortly before cells divide, it shortens and thickens into more distinct and visible chromosomes. These are responsible for the transmission of traits or characteristics to new cells or offspring. 2. Endoplasmic Reticulum smooth endoplasmic reticulum rough endoplasmic reticulum Electron microscopes have nucleus revealed networks of channels which extend throughout the cytoplasm. These appear to be formed by a series of infolding membranes extending from the nuclear membrane to ribosomes the outer plasma membrane. There is still some uncertainty as to their actual formation. One theory suggests that the membranous infoldings arise from the nuclear membrane and extend outwards to the plasma membrane – perhaps even contributing to its formation as a cell grows. The entire system of infoldings and channels is known as the endoplasmic reticulum. In some cells they are quite numerous, while in others they appear to be absent. The membranes may have many small bodies called ribosomes attached to them while in other areas they may be relatively free of them and smooth. Biology 30 18 Lesson 1 The varied nature of the endoplasmic reticulum makes it appear to be responsible for a number of actions: biochemical activities such as the production and storage of lipids; where ribosomes are common, it seems to be an area of protein formation; where there are few ribosomes, enzymes and other substances are thought to be produced; throughout, the network of channels may allow the movements of substances between the outer membrane and the nucleus. Therefore, the network appears to function as an area where manufacturing occurs as well as transportation and possible storage of substances. 3. Ribosomes Tiny spheres, or ribosomes, are found scattered throughout cells as well as being attached to the endoplasmic reticulum. In the latter instance, rough endoplasmic reticulum indicates that there are many attached ribosomes while smooth endoplasmic reticulum means there are few or no attached ribosomes. These small spherical organelles contain enzymes and ribonucleic acid (RNA) which are involved in the process of putting amino acids together to form proteins. The proteins may then move through the endoplasmic reticulum to other parts of the cell or out of the cell and into other parts of the body. 4. Golgi Complexes The Golgi material is a series of membranes which has some resemblance to the endoplasmic reticulum. (There is some speculation that Golgi Complexes may even be formed by splitting off from the endoplasmic reticulum.) They appear somewhat like flattened discs or balloons pressed together with small sacs around the edges. Golgi complexes Golgi complexes together with the endoplasmic reticulum packages proteins and exports them to other locations in the cell. Golgi complexes are especially common in some secretory cells. Biology 30 19 Lesson 1 5. Mitochondria Mitochondria are oval-shaped organelles which are believed to be the "powerhouses" of cells. Each is surrounded by a double membrane with the inner membrane forming folds called cristae. Enzymes on these cristae act on carbon compounds, during the process of respiration, to release energy for numerous cell activities. Mitochondria are especially numerous in active cells, such as those of the (heart) muscles. 6. Lysosomes Approximately the same size and shape as mitochondria, these round "containers" tend to be more numerous in animal cells. Some plant cells may not even have any. Lysosomes function as storage vessels for powerful digestive enzymes in cells. Foreign particles, such as bacteria, worn-out cell parts and food particles fuse to, and then are enclosed by, the membrane of a lysosome. Its digestive enzymes then break down the particles and release energy. Lysosomes are believed to be formed by small sacs pinching off from the Golgi Complexes. These round bodies are sometimes called "suicide sacs". If a cell dies or is injured in any way causing the containing membranes of lysosomes to release their enzymes, those enzymes will begin breaking down the cell itself. In some instances, lysosomes seem to be "programmed" to release their enzymes at certain times. Not frequent, this sometimes happens as in the life cycle of frogs where the tail degenerates. Enzymes break down the tail cells and the cell remains are resorbed as a tadpole changes into a frog. In other instances, release of the enzymes may also occur in order to break down organelles or even cells themselves as they wear out. This allows for cell replacements. Biology 30 20 Lesson 1 7. Centrosomes (Centrioles) centriole lysosome Most animal cells and only a few plant cells show centrosomes or rounded bodies near the nuclei. Some references do not even use the term "centrosome", which they regard as more dense cytoplasm surrounding a pair of centrioles. cytoskeleton The centrioles exist as a pair of rod-like organelles which are at right angles to each other. One centriole resembles a circular bundle of nine rods or microtubules, each consisting of three smaller rods or microtubules. Centrioles appear to be involved in cell division. Before a cell divides, a pair of centrioles will duplicate and separate so that two pairs are formed. The two pairs of centrioles then move to opposite sides of the cell. Threadlike spindle fibers develop between the two pairs of centrioles. Chromosomes attach to these, become separated and are pulled to opposite ends of the cell just before it divides. There is still some uncertainty about the role of centrioles, since they appear to be absent in higher plants and yet spindle fibers are still formed. There is also some speculation that centrioles may somehow be involved in the development of cilia or flagella on some cells, which are structures associated with movement. 8. Cytoskeleton – is composed of microtubules and microfilaments While the structures making up centrioles appear to be solid rods rather than tubes, there are other hollow tubes or filaments found throughout the cytoplasm of many cells. Made of protein, the scattered nature of these tubules throughout the cytoplasm may form an almost skeleton-like structure internally. This framework may give shape and some organization to cells. In addition, the tubules could be associated with some internal movements or transport. Biology 30 21 Lesson 1 9. Vacuoles vacuole Vacuoles, which are fluid-filled spaces, occur in both plant and animal cells. In plants they are often quite prominent, taking up a large part of the middle of a cell and pushing the cytoplasm and the other organelles to the outer edges. These vacuoles serve as reservoirs for a variety of substances: pigments, salts, food particles and wastes. Plant vacuoles tend to be permanent, remaining throughout cells' lives. Some vacuoles found in certain animal cells, such as the contractile vacuoles which regulate water balance in some protozoans, are also permanent. However, other animal vacuoles which may contain food or act as storage areas, are not usually as prominent as those in plants and are often only temporary. The above image illustrates a vacuole in an animal cell, which is much smaller than those found in plant cells. 10. Plastids These organelles are only present in the cytoplasm of plant cells and some protists with some plant-like characteristics. Some have internal structures which are quite complex of which a chloroplastid is an example. Chloroplastids or chloroplasts contain the green pigment chlorophyll, which is necessary in converting or capturing the energy of sunlight into carbohydrate form. Orange (carotene) and yellow (xanthophyll) pigments are other By Thomas Dreps pigments commonly found in chloroplasts. (The changing of leaf color in autumn is essentially the result of the breakdown of green chlorophyll which had previously covered up the yellows and oranges. Red pigments also appear in reactions where sugar had been previously produced.) Chromoplasts are plastids containing not only orange and yellow pigments, but reds and blues. These frequently show up in the epidermal cells of flowers and fruits. Leucoplasts are plastids designed for storing substances such as starches. When energy is required the (insoluble) starches are changed to (soluble) sugars, which can then be transported by plant fluids to wherever they are required. Potato tubers are particularly plentiful in leucoplasts. Biology 30 22 Lesson 1 Emphasizing some Plant and Animal Cell Differences In the descriptions given of cell parts and organelles, references were made to certain parts being present or absent, or of a different form in one group of organisms as compared to the other. Many plant cell organelles are nearly identical to those in animal cells. Following, is a summary of probably the four distinct differences between the cells of advanced plants and animals. 1. Plants have fairly thick (in proportion to total cell sizes) outer cell walls. The thickness and nature of the cellulose fibers making up a cell wall result in plant cells commonly having rigid and specific shapes. Animals do not have cell walls. Animal cell contents are enclosed only by thin, flexible cell membranes. 2. Plant cells are usually dominated by large, permanent and centrally located vacuoles. Animals have small scattered vacuoles which may appear and disappear as food and waste conditions change. 3. Plastids, and especially chloroplasts, are common features of plant cells. They are not found in higher animal cells. 4. Centrioles are found in animals cells but not in plant cells. Plant and animal cells contain many of the same membrane enclosed organelles (eukaryotic cells) and structures. Some of these organelles in common are: the nucleus, mitochondria, endoplasmic reticulum, golgi apparatus, lysosomes, the cytoskeleton and cytosol. Biology 30 23 Lesson 1 Cell Division-Cell Continuity Cells and the organelles they may contain within them cannot continue to grow or to live indefinitely. As will be seen in the next lesson, increasing size makes it more and more difficult for an individual cell to satisfy its nutrient requirements or to remove all wastes. At the same time, internal matter and organelles must be replaced or repaired to maintain efficient functioning. Cell division or reproduction occurs either on a regular or an irregular basis to meet these needs. In order for cell reproduction to be meaningful or successful, at least two actions must take place. 1. The genetic material, which determines the nature of a cell and how it functions, must be duplicated (or replicated). 2. The cytoplasmic contents must then be equally distributed among the "new" or daughter cells if they are to function successfully. The processes associated with the reproduction, growths and functionings of cells follow a general pattern which makes up what is known as the "cell cycle". This will be looked at more closely in the next lesson. Organization of Living Material In watching young children, or even adults, examining objects which may be new and interesting to them, they will commonly be seen to take things apart to examine pieces individually. The same may be done with living matter – although once the smallest parts are reached and examined, they are not likely to be living anymore! With the aid of certain techniques, dissecting instruments and microscopes, one should be able to divide the matter of a living organism down to the smallest unit, which is the cell. Cells could possibly be broken down further into organelles, compounds and elements but individually, these are either non-living or would not be able to sustain life on their own for very long. Some organisms are unicellular, existing as single cells which can function independently of other cells. Some of these were examined in Biology 20 during the taxonomic studies. In certain instances cells are colonial, where they live in groups (as some yeasts, bacteria, algae and other organisms do) but are still really independent. Biology 30 24 Lesson 1 Multicellular organisms show higher degrees of organization and divisions of labor in the living matter. While the simplest units are still cells, these become specialized and grouped in particular ways to carry out certain functions. Thus, more advanced animals may have bone cells, nerve cells and muscle cells among others. These cells are dependent on the others to work together to function. Similar cells, which together perform the same type of job, are called tissues. Ex: nerve and muscle tissue, or in plants, palisade cells. Different tissues grouped together to perform one function become an organ. Ex: heart, kidney, liver or stomach. In plants, xylem and phloem are organs. Organs are then grouped into specialized systems. Ex. Digestive, circulatory and respiratory systems. In plants, examples are vascular bundles and leaves. Finally, when all organ systems are functioning together, there is a complete organism. Ex. A human or a plant. Beyond the organism level, one can find other organizations or relationships of living matter on ever-broader levels. These may be reviewed as: Population - This includes a number of organisms of one kind or one species in a particular place. (Eg. Elk in the Moose Mountain area.) Community - Organisms of different species in a certain area make up that area's community. (Eg. Frogs, muskrats, cattails, etc. in a marsh.) Ecosystem - Relationships involving living and non-living factors in a particular environment make up an ecosystem. Biosphere - Finally, biosphere refers to all the living organisms around this planet. Biology 30 25 Lesson 1 Summary The basic building and functioning unit of a living organism is a cell. With a few exceptions, the cells of most organisms show three recognizable parts or divisions: 1. Cell wall and/or cell membrane. 2. Cytoplasm. 3. Nucleus (or nucleoplasm). As a summary and study aid, the following table lists some of the parts and organelles, as well as their functions. Structure Function Cell wall Support and protection. Cell membrane Regulates movements of substances in and out of a cell. Nucleus Directs the synthesis or reproduction of proteins. Cytoplasm Contains the organelles and the numerous substances taking part in, or which are the result of, various life-sustaining actions. Endoplasmic reticulum Manufacturing and storage area as well as a transporting system. Ribosome Arranges amino acids into proteins. Golgi complex Secreting, "packaging" and storing various substances, as well as transport. Mitochondrion Releases energy for cell use. The "powerhouse". Lysosome Encloses powerful digestive enzymes. Centrosome Contains centrioles, which take part in animal cell division. Vacuole Storage or holding area for water, food, salts and wastes. Plastid Captures and stores energy or stores pigments or food substances. Biology 30 26 Lesson 1 The discovery and accumulation of cell knowledge is not only interesting in itself but, as will be seen in the remainder of the course, it can be used to help explain the many processes occurring in living things. Much of this knowledge can be applied to practical uses in such diverse fields as: plant and animal reproduction and growth; plant and animal diseases and possible treatments; and, the general area of genetics. Probably of particular interest to ourselves is information of the type related to hereditary diseases and other kinds of illnesses, organ transplants and a topic which has been intriguing generations before us: how and why aging (of cells and organisms) occurs and how it may be slowed (or even stopped). Biology 30 27 Lesson 1