Parasite/ Fungal lab study guide (MAIN)
Slide#12 Entamoeba histolytica
Focus w/ 100X objective and use oil.
See fecal smear with round cells w/ prominent nucleus
(chromatin thickening). Often see 2 nuclei due to cell
division.
Type of microbe/ characteristics: amoeba, protozoan
Domain: Eukarya
Kingdom: Formerly Protista
Genus: Entamoeba
Species: histolytica
Disease: Amoebic dysentery
Slide # 13 Trypanosoma gambiense
Focus w/ 100X objective and use oil.
See red blood cells with organism outside of RBC.
Type of microbe/ characteristics: protozoan with flagella &
undulating membrane
Domain: Eukarya
Kingdom: Formerly Protista
Genus: Trypanosoma
Species: gambiense
Common name: --Disease: African Sleeping Sickness
Slide # 14 Plasmodium sp.
Focus w/ 100X objective and use oil.
See red blood cells with organism inside RBC, “signet ring”
(A ring with a birthstone).
Type of microbe/ characteristics: protozoan
Domain: Eukarya
Kingdom: Formerly Protista
Genus: Plasmodium
Common name: --Disease: Malaria
Ascaris lumbricoides
*Plastic mount, not slide.
Type of microbe/ characteristics: roundworm
Domain: Eukarya
Kingdom: Animalia
Phylum: Nematoda
Genus: Ascaris
Species: lumbricoides
Disease: Ascariasis. Largest and most common
parasitic worm in humans.
Slide # 15 Taenia pisiformis
Focus w/ 4X only. Lower condenser, close diaphragm.
See scolex (head w/ hooks & suckers), proglottid (segment),
gravid proglottid (ovary branches filled w/ eggs), testes
are present but not visible. Tapeworms are
hermaphroditic.
Type of microbe/ characteristics: flatworm
Domain: Eukarya
Kingdom: Animalia
Phylum: Platyhelminthes
Genus: Taenia
Species: pisiformis
Common name: tapeworm
Disease: Parasite infection
Slide # 16 Clonorchis sinensis
Focus w/ 4X only. Lower condenser, close diaphragm.
See “small leaf”, oral sucker, ventral sucker, egg filled
uterus, testes are pink at posterior end.
Type of microbe/ characteristics: flatworm
Domain: Eukarya
Kingdom: Animalia
Phylum: Platyhelminthes
Genus: Clonorchis
Species: sinensis
Common name: Chinese liver fluke
Disease: Parasite infection
Slide # 17 Fasciola hepatica
Focus w/ 4X only. Lower condenser, close diaphragm.
See “large leaf”
Type of microbe/ characteristics: flatworm
Domain: Eukarya
Kingdom: Animalia
Phylum: Platyhelminthes
Genus: Fasciola
Species: hepatica
Common name: liver fluke
Disease: Parasite infection
Slide #18 Necator americanus
Focus w/ 4X only. Lower condenser, close diaphram
See hook at anterior end
Type of microbe/ characteristics: roundworm
Domain: Eukarya
Kingdom: Animalia
Phylum: Nematoda
Genus: Necator
Species: americanus
Common name: hookworm
Disease: parasite infection
Slide # 19 Trichinella spiralis
Focus w/ 4X only. Lower condenser, close diaphragm.
See curled up worms in muscle fibers, sometimes encysted.
Type of microbe/ characteristics: roundworm
Domain: Eukarya
Kingdom: Animalia
Phylum: Nematoda
Genus: Trichinella
Species: spiralis
Common name: --Disease: Trichinosis
Slide # 20 Enterobius vermicularis
Focus w/ 4X only. Lower condenser, close diaphragm
See
Type of microbe/ characteristics: roundworm
Domain: Eukarya
Kingdom: Animalia
Phylum: Nematoda
Genus: Enterobius
Species: vermicularis
Common name: pinworm
Disease: parasite infection
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Slide # 21 Saccharomyces sp., budding
Focus w/ 100X objective and use oil.
See single-cell yeasts, some reproducing asexually by
budding. Pseudohyphae.
Type of microbe/ characteristics: yeast (fungi)
Domain: Eukarya
Kingdom: Fungi
Phylum (Division): Ascomycota
Genus: Saccharomyces
Common name: yeast
Disease: Yeast in general can cause dermal
infection, vaginal infection
Slide # 22 Aspergillus sp., conidia
Focus w/ 10X, 40X.
See “dandelions”
Type of microbe/ characteristics: fungi
Domain: Eukarya
Kingdom: Fungi
Phylum (Division): Ascomycota
Genus: Aspergillus
Common name: --Disease: Farmer’s lung (from moldy hay),
aflatoxin in peanuts from Aspergillus flavus
Slide # 23 Penicillium sp. conidia
Focus w/ 100X objective and use oil.
See “skeleton hands”
Type of microbe/ characteristics: Fungi
Domain: Eukarya
Kingdom: Fungi
Phylum (Division): Ascomycota
Genus: Penicillium
Common name:--Disease: none- produces antibiotic
Slide # 24 Rhizopus stolonifera
zygospores & sproangia
Focus w/ 10X, 40X.
See “Tootsie pops”, non-septate hyphae (no cross walls),
zygospores- sexual spores,
sporangia- house asexual spores
Type of microbe/ characteristics: Fungi
Domain: Eukarya
Kingdom: Fungi
Phylum (Division): Zygomycota
Genus: Rhizopus
Species: stolonifera
Common name: Black bread mold
Disease: nasal/ pharyngeal infection in people w/
uncontrolled diabetes
Slide # 25 Peziza sp.
Focus w/ 10X, 40X.
See “pizza crust”, ascocarp with asci
Type of microbe/ characteristics: Fungi
Domain: Eukarya
Kingdom: Fungi
Phylum (Division): Ascomycota
Genus: Peziza
Common name: --Disease: none
Slide # 26 Coprinus sp.
Focus w/ scanning, 10X, 40X.
See basidiocarp (mushroom cap) cut horizontally (like sun
with reverse flames), or vertical slice, Gills with
basiodiospores
Type of microbe/ characteristics: Fungi
Domain: Eukarya
Kingdom: Fungi
Phylum (Division): Basidiomycota
Genus: Coprinus
Common name:--Disease: none
Slide # 27 Oscillatoria sp.
Focus w/ 10X, 40X.
See green filaments, some long & thin, some stout and wide.
Type of microbe/ characteristics: It is single cell bacteria
which are living side by side.
Domain: Bacteria
Kingdom: Bacteria
Phylum: Cyanobacteria
Genus: Oscillatoria
Common name: blue green algae (but not algae).
Disease: none
Slide # 28 Giardia lamblia
cyst
See power point picture. Don’t view slide.
See “Old man with a moustache”
Type of microbe/ characteristics: Protozoan
Domain: Eukarya
Kingdom: Formerly known as Protista
Genus: Giardia
Species: lamblia
Common name: --Disease: Giardiasis
Slide # 29 Trichomonas vaginalis
Focus w/ 100X objective and use oil.
See “alien eyes”, 5 anterior flagella
Type of microbe/ characteristics: Protozoan
Domain: Eukarya
Kingdom: Formerly known as Protista
Genus: Trichomonas
Species: vaginalis
Common name: --Disease: Vaginitis
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Domain Bacteria
Kingdom: Bacteria
Phylum: Bacteria
(All bacteria we have
studied in slides 3-11, but
not included in Test 2
material.)
Phylum: Cyanobacteria
Oscillatoria sp.
Domain Archaea
Extremophile
bacteria
Domain Eukarya
Kingdom: Formerly Protista
Entamoeba histolytica
Trypanosoma gambiense
Plasmodium sp.
Trichomonas vaginalis
Kingdom: Animalia
Phylum: Nematoda
Ascaris lumbricoides
Necator americanus
Trichinella spiralis
Enterobius vermicularis
Phylum: Platyhelminthes
Clonorchis sinensis
Fasciola hepatica
Taenia pisiformis
Kingdom: Fungi
Phylum: Ascomycota
Saccharomyces sp.
Aspergillus sp.
Penicillium sp.
Peziza sp.
Phylum Zygomycota
Rhizopus stolonifera
Phylum Basidiomycota
Coprinus sp.
1. DNA sequencing is now clarifying the similarity and differences among
organisms for classification.
2. Phylum Platyhelminthes = flatworms. “Plate” or “Platy is flaty”.
3. Phylum Nematoda = roundworms.
4. Kingdom Protista is being divided into several kingdoms, but taxonomists have
not agreed on what those will be.