A. Rosado / Page 1 of 2 Arelys Rosado 4725 Sheboygan Avenue #229 Phone: (787) 220-3293 Email: arosado@wisc.edu _____________________________________________________________________________ OBJECTIVE To seek position in the growing organization where I can utilize my innovative ideas, experience for accomplishing the projects in the field of biotechnology and chemical biology. I would like to exploit my potential, quality of education and sense of responsibility for amplifying the profit of the company and elevating the reputation of the company. QUALIFICATION SUMM AR Y •Over 7+ years of hands-on experience in multidisciplinary scientific background including polymer and inorganic compound synthesis, purification, and characterization; general microbiology and molecular biology; fluorescence lifetime and anisotropy, steady-state spectroscopy •Excellent verbal and written communication and organization skills •Successfully manage time and multiple projects; set priorities and meet deadlines demonstrated over several leadership positions LABORATORY SKILLS Chemical Synthesis: Polymers and inorganic compounds Analytical and physical spectroscopy: Time-resolved fluorescence lifetime and anisotropy; steady-state spectroscopy. Electrochemistry (Square Wave and Cyclic Voltammetry). UV-Vis. FTIR. Atomic Force Microscopy. Chromatography (Silica, Alumina, Sephadex and Thin Layer Chromatography) Molecular biology and microbiology: Media preparation and cell culturing techniques. Plasmid preparation, digest analysis, DNA sequencing. In-vitro transcription/translation system. E.coli cellextract. tRNA aminoacylation and derivatization. DNA modification. Electrophoresis (SDS-PAGE, native, agarose) EDUCATION Ph.D. Chemical Biology, University of Wisconsin-Madison, USA, September, 2010-December, 2015 B.S. Chemistry, University of Puerto Rico-Rio Piedras, San Juan, PR, May 2010 RESEARCH EXPERIENCE Research Assistant, UW-Madison; Madison, Wi — June 2010-Present •Performed experiments to study the co-translational protein folding on the cell using FRET measurement to determine the degree of compaction of ribosome-bound nascent proteins •Incorporated fluorophores at specific sites co-translationally and measured and analyzed steadystate and time-resolved frequency-domain fluorescence and anisotropy Research Assistant, UPR-Rio Piedras; San Juan, PR — June 2006-August 2010 •Conducted research on the development of an electrochemical DNA biosensor for Salmonella detection •Immobilized DNA sequences over a polymer films and characterized by AFM, XPS and electrochemical techniques Research Assistant, Columbia University; New York, NY — June 2008-August 2008 •Study the interplay between bacterial and viral transcription termination factor NusA Research Assistant, UPENN; Philadelphia, PA — June 2007-August 2007 •Study the temperature dependence of domain polarization on BaTiO₃ thin film and single crystal using AFM A. Rosado / Page 2 of 2 PUBLICATIONS Madeline Díaz Serrano, Arelys Rosado, Joselyn del Pilar, Esther Vega and Ana R. Guadalupe. “Design, Construction and Analytical Characterization of a DNA Sensor for the Detection of Salmonella”, Polymeric Materials Science and Engineering, 2008, 98, 448-449. Madeline Díaz Serrano, Arelys Rosado, Joselyn del Pilar, Melvin Arias and Ana R. Guadalupe.”A Polymer-Based Electrochemical DNA Biosensor for Salmonella: Preparation, Characterization and Calibration”, Electroanalysis, Manuscript Accepted January 2011. SELECTED PRESENTATIONS (2 OUT OF 13) Protein Folding Consortium Workshop, Berkley California, June 2011 Annual Biomedical Research Conference for Minority Students, St. Louis, Missouri, November 2011 TEACHING AND LEADERS HIP SKILLS Teaching Assistant, Department of Chemistry, Fall 2010 •Provided technical advice to 50 students working on basic laboratory techniques •Applied different teaching strategies to insure progress of each individual •Created an environment conductive for teamwork and cooperation Recruiter, Department of Chemistry, 2010-2012 •Attended three national conferences to recruit Chemistry prospective students •Helped organize and bring the recruitment material •Talked to prospective students about what the department and university have to offer •Provided follow-up with the prospective students afterwards Translator, First Baptist Church of Caguas, Puerto Rico, 2008 •Organized a missionary trip to Chile •Arranged activities for 30+ people in a wide range of ages •Provided consecutive or simultaneous translation between languages (Spanish and English) for two consecutive weeks •Translated 25+ documents and other materials from Spanish to English and vice versa Board of Directors (Treasurer), First Baptist Church of Caguas, Puerto Rico, 2003-2007 •Developed and oversaw weekly meetings for 30+ teenagers and young adults; double the number of members by the end of my term •Verified accuracy of transmittals; issued receipt noting appropriate account number. Clarified cash balancing and transmittal procedures •Arranged several fundraiser activities. Balanced total cash and checks received to receipts; deposit with bank and recorded final statement COMPUTER AND LANGUAG E SKILLS Solid background in Microsoft Office, Mac iWork, EndNote, instrument control and data acquisition software Spanish (native language) English (fully proficient with excellent oral and communication skills) SELECTED HONORS AND AWARDS (2 OUT OF 6) Biotechnology Training Program Graduate Research Fellows, UW-Madison, 2011-Present NIH-RISE Undergraduate Research Fellows, UPR-Rio Piedras, 2006-2010