Amgen Biotech Experience Materials Request Form
Name of School: __________________________________
Your Phone _________________
Name of Contact Teacher and Email address: ______________________________________________________
List other teachers doing the labs and their email addresses__________________________________________
TOTAL NUMBER OF STUDENTS DOING THE ABE LABS AT YOUR SCHOOL :_______
Reservation/Pick-up Date: _______________
Equipment+Supplies ____
Supplies only____
Visit our website: www.amgenbiotechexperience.com for errata pages, videos and important tips.
Total Number of Students doing any of the ABE labs:______
Total Number of Classes doing any of the ABE labs:_______
Select the Pathway your students will be doing and fill in the requested numbers
Complete Genetic Engineering Sequence (Labs 1, 2, 3, 4, 5, and if you want 6)
Description: This laboratory sequence tells the “complete” biotechnology story from moving a gene of interest into an engineered
expression vector. This sequence involves the digestion of pARA and pKAN-R followed by a ligation lab to produce recombinant plasmids.
This represents the most challenging set of protocols and includes Purifying the Fluorescent Protein (Lab 6) if you choose to do so.
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Number of Students running this sequence
Number of groups running this sequence
Number of teachers teaching this sequence
Lab 6
___ number of groups
Abridged Genetic Engineering Sequence (Labs 1, 2a, 4a, 5a, and if you want 6)
Description: This sequence omits the pARA/pKAN-R digestion and ligation of restriction fragments, and starts with the rfp gene already
ligated into the expression vector. Students run a plasmid digest and electrophoresis separating pARA-R restriction fragments. This was
written to shorten the labs required to get to protein purification Lab 6 and to increase the likelihood of students succeeding in producing
red transformants. The sequence requires less teacher preparation time.
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Number of Students running this sequence
Number of groups running this sequence
Number of teachers teaching this sequence
Lab 6
___ number of groups
Focus on Bacteria Sequence (Labs 1, 5b, and if you want 6)
Description: The focus of this sequence is on the importance of plasmids as vectors for use in genetic engineering and the ability of
bacteria to utilize the plasmid’s genetic information to produce a protein product. It is not only important to produce the protein, it is
equally important that the protein be capable of being collected and purified.
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Number of Students running this sequence
Number of groups running this sequence
Number of teachers teaching this sequence
Lab 6
___ number of groups
Introduction to Biotechnology (Lab 1)
Description: The focus of this lab is to enable the students to become proficient in basic biotechnology lab skills. It includes using the
micropipette and running a gel electrophoresis.
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Number of Students running this sequence
Number of groups running this sequence
Number of teachers teaching this sequence
Genomic DNA Extraction and PCR
Description: These labs are independent and are not considered part of the Pathways. If the teacher has attended an Amgen Biotech
Experience workshop which included PCR training, he/she may choose to include these labs with the Pathway sequence selected or to do
these as an independent lab experience.
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Number of Students running this sequence
Number of teachers teaching this sequence
**RETURN THIS FORM TO YOUR SITE COORDINATOR at least ONE MONTH BEFORE YOUR RESERVATION DATE
3/7/2016