Shujsky V.F., Maksimova T.V., Petrov D.S., Lvutina N.V., Kuznetzova D.S., Klavdiev I.A. Experience of the isobolic method use for evaluation and regulation of multifactor mining enterprises impacts on Leningradsky region river ecosystems // Modern problems of aquatic toxicology. International Scientific Conference.– Borok, Sept. 20– 24, 2005 (in print). __________________________________________________________________ EXPERIENCE OF THE ISOBOLIC METHOD USE FOR EVALUATION AND REGULATION OF MULTIFACTOR MINING ENTERPRISES IMPACTS ON LENINGRADSKY REGION RIVER ECOSYSTEMS Shujsky V.F., Maksimova T.V., Petrov D.S., Lvutina N.V., Kuznetzova D.S., Klavdiev I.A. Saint-Petersburg State Mining Institute (technical university) 199106, Saint-Petersburg, 21-st line, 2. E-mail: shuisky@vs4146.spb.edu Universal quantitative evaluation and standard-setting method of complex (multifactor) impacts on fresh-waters ecosystems is developed and approbated. The suggested method uses the revealed general laws of the best hydroecosystem bioindicator reaction - macrozoobenthocoenoses to multicomponent external impact, which is estimated by the original isobolic index. The present techniques and data are based on representative long-term hydroecological research results executed by the group of authors. The approbation of this method is realized by the example of complex technogenic mining enterprises impacts on especially vulnerable highest valued fish-economic river ecosystems. They are the Luga River (under the Limited Liability Company "Industrial Group "Phosphorite" impact), the Plussa River (the Joint-stock Companies "Leningradslanets", "Tsesla", "Shale processing plant" and other), the Syas River (the Joint-stock Company "Boksitogorsky alumina" and "pikalevsky alumina") and its inflows. In these situations technogenic eutrophication of river is burdened by active reaction changes and complex (multifactor) contamination. The isobolic method use allowed: to set the maximum permissible levels of these combination impacts on biota considering real factor interactions; to realize adequate quantitative impact evaluation as the rate of excess by these impacts of its maximum permissible level; to analyze environmental risk and predict most probable technogenic damage; to develop quite effective environmental protection measures planning quantitatively its results. The theoretical basis and detailed description of the method are presented in collective monograph "The isobolic method of multifactor anthropogenic impact on fresh-water ecosystems evaluation and standard-setting by macrozoobenthos state " (СПб.: IAEMNPS, 2004, 304 p.; № of state registration 05-12240). Practical applications of this method are illustrated in detail by the factual data. Particular examples of the method used for solving various environmental protection problems are also considered.