Tentative plan for master and PhD course ”Nutrient Absorption and Metabolism” – summer 2013 All lectures will be given in data room 1 (room no 2002 in building C22). Week 32: 5/8: Introduction to the course. Presentation of participants, teachers. Introduction to the aim and hypotheses of the scientific project, planned analyses, animal model and blood sampling. Define groups. (Peter Theil). Review of surgery, sampling collection/number system etc. (Adam Storm). Registration of various things, passwords etc (Lars Gildbjerg). 6/8: Introduction to the animal model / scientific project (continued), and presentation of blood supply/anatomy at positions of permanent catheters (Peter Theil). Techniques to measure blood flow (Adam + Uffe Krogh Larsen) + calculations. Preparation of animal experiment. Amounts of blood sampled (calculate max drainage), collection and stabilization of collected plasma (Helle Lærke 11:00 – 12:00). 7/8: In vivo animal experimentation – documentation and reporting. (Mette Herskin 8:15-9:00). Regulation of blood flow (Niels Oksbjerg 9:00 – 12:00). Visit to the barns (SB37, SB38 and K36) and excursion. 8/8: Team 1: Blood profile (K36) – Peter Theil/Adam Storm. Team 2: Formulate a project with catheterized animals (Group discussion; Peter Theil) + Exercise/calculations of whole animal metabolism (Mogens Larsen 8:15-9:00). Team 3: Compare behavior of animals exposed to +/- surgery (Mette Herskin 8:30 – 11:30) + Exercise/calculation of portal recovery of HMB. 9/8: Team 2: Blood profile (K36) – Peter Theil/Adam Storm. Team 3: Formulate a project with catheterized animals (Group discussion; Peter Theil) + Exercise/calculations of whole animal metabolism (Mogens Larsen 8:15-9:00). Team 1: Compare behavior of animals exposed to +/- surgery (Mette Herskin 8:30 – 11:30) + Exercise/calculation of portal recovery of HMB. Week 33: 12/8 Team 3: Blood profile (K36) – Peter Theil/Adam Storm. Team 1: Formulate a project with catheterized animals (Group discussion; Peter Theil) + Exercise/calculations of whole animal metabolism (Mogens Larsen 8:15-9:00). Team 2: Compare behavior of animals exposed to +/- surgery (Mette Herskin 8:30 – 11:30) + Exercise/calculation of portal recovery of HMB. 13/8: Theme: Fibre and VFA metabolism Dietary fiber – classification and analysis (Helle Lærke 8:15-9:00) Quantitative digestion – Methods, nutrients, and exercise (Peter Lund 9:15-11:00). Morning 2: Regulation of blood flow and measurements of VFA absorption from the rumen + other experiments with multicatheterised cows (Adam Storm 11:15-13:30 incl. break). Afternoon: Work with lab analyses and data. 14/8: Theme: Protein and amino acid metabolism Morning 1: Application of isotopes in studies of absorption and metabolism (Mogens Larsen 8:15-10:00). Morning 2: Protein and amino acid requirements, recommendations, dietary supply, categories of AA (essential, branch-chained, sulphuric AA, glucogenic/ketogenic, acidic/basic). (Jan 15/8 16/8 Værum Nørgaard 10:15-12:00). Inter-organ fluxes of AA (Adam Storm/Betina Røjen Amdisen 12:30-13:30) Afternoon: Work with lab analyses and data. Theme: Carbohydrate metabolism Morning 1: Combination of multicatheterised sows and metabonomic studies (Mette Hedemann 8:15 – 10:00) Morning 2: Absorption and metabolism of phytochemicals (Knud Erik Bach Knudsen 10:1512:00). Nutrition and insulin secretion (Anne Krog Ingerslev 12:30-13:15) Afternoon: Work with lab analyses and data. Theme: Nutrition and intermediary metabolism of sows Morning 1: Nutrition of the sow with focus on late gestating sows and quantification of colostrum / milk intake using D2O (Peter Theil). Prediction of milk yield (Anja Varmløse Hansen). Morning 2: Experiments with multicatetherized pigs and sows (Peter Theil) Afternoon: Work with lab analyses and data. Week 34: 19/8: Work with lab analyses and data+report writing. 20/8: Work with lab analyses and data+report writing. 21/8: Work with lab analyses and data+report writing 22/8: Work with lab analyses and data+report writing. Deadline 12:00 for written report. Afternoon: Preparation of oral presentation. 23/8: Morning: Oral presentation and plenum discussion. Afternoon: Oral examination. Evaluation of “NAM 2013”-course. Teachers: Peter K. Theil, senior scientist Adam C. Storm, post doc Guest lecturers: Uffe Krogh Larsen, phd student Helle Lærke, senior scientist Mette S. Herskin, senior scientist Niels Oksbjerg, senior scientist Mogens Larsen, post doc Peter Lund, senior scientist Jan V. Nørgaard, lector Betina Røjen Amdisen, post doc Anne Krog Ingerslev, phd student Mette Hedemann, senior scientist Knud Erik Bach Knudsen, professor Anja Varmløse Hansen, phd student