CURRICULUM VITAE NIDARSHANA CHATURVEDI PARASHAR Department of Biochemistry, Panjab University, Sec-14, Chandigarh (UT) . 160014 E-mail : nidarshanachaturvedi@gmail.com Phone No: + 91-8558874981, +91 9646178481 RESEARCH EXPERIENCE Having 7 years research experience as a Research Intern, project fellow and PhD scholar in Genetic Resources and Biotechnology Division at Central Institute of Medicinal and Aromatic Plants (CIMAP), CSIR. Worked as PhD fellow with CSIR-Senior Research Fellowship (CSIR-SRF) in Biotechnology Division at Central Institute of Medicinal and Aromatic Plants (CIMAP), CSIR till Jan 2012 and submitted PhD thesis at Biochemistry division, Lucknow University, Lucknow. Worked as Research fellow in Biochemistry division, Post Graduate Institute of Medical Research and Education (PGIMER), Chandigarh (2013). Currently working as DBT-Research Associate in Department of Biochemistry, Panjab university, Chandigarh. PUBLICATIONS: Published: 1.) Impact of Promising Genotypes of Papaver somniferum Developed for Beneficial Uses. Mridula Singh#, Nidarshana Chaturvedi#, Ajit K. Shasany and Ashutosh K. Shukla. Communicated to Acta Horticuturae (2014). 2.) Comparative analysis of Papaver somniferum genotypes having contrasting latex and alkaloid profiles. Nidarshana Chaturvedi, Mridula Singh, Ashutosh K. Shukla, Ajit K. Shasany , Karuna Shanker, Raj K Lal and Suman PS Khanuja. Protoplasma, (2013). doi:10.1007/s00709-013-0587-7 3.) Latex less opium poppy: cause for less latex and stem strength. Nidarshana Chaturvedi, Ashutosh K. Shukla, Raj K Lal , Madan M Gupta ,Upendra N Dwivedi and Ajit K. Shasany. Physiologia Plantarum, (2013). doi:10.1111/ppl.12086. 4.) Expression of CspE in Enterobacter ludwigii PAS1, psychrotroph from Indian Himalayan soil and in silico protein modelling, prediction of conserved residues and active sites. Premalatha Kandasamy, Nidarshana Chaturvedi, Brijesh S. Sisodia, Ajit K. Shasany, Shachi Gahoi, Soma S. Marla, Reeta Goel. Current Microbiology (2013), 66 (5), 507-14. 5.) Curcumin causes promoter hypomethylation and increased expression of FANCF gene in SiHa cell line. Gaurav Parashar, Nidarshana Chaturvedi Parashar, Neena Capalash. Molecular and Cellular Biochemistry (2012), 365, 29-35. 6.) Registration of a high oil yielding variety “CIM- Vriddhi” of vetiver (Vetiveria zizanioides, Linn. Nasha). Chauhan HS, Lal RK, Khanuja SPS, Rane S, Tandon S, Chanotiya CS, Yadav AK, Singh AK, Singh HP, Darokar MP, Chandra,Ram, Singh Kailash, Singh HN, Gupta AK, Kalra A, Bansal,RP, Bahl JR, Singh AK, Misra AN, Dhawan OP, Singh SP,Patra DD, Alam M, Gupta MK, Chaturvedi N, Kumar D,Misra HO, Lohia RS, Bansal K, Shankar H, Pandey Rakesh,and Singh Vikram. Jour. Med. Arom. Pl. Sci. (2008), 30, 427-429. 7.) An alpha-1 antitrypsin genetic variant from India. Ajit K Shasany, Ashutosh K Shukla, Mahendra P Darokar, Megha Saraiya, Nidarshana Chaturvedi, Lankeshwar Tewari and Suman P S Khanuja. Indian Journal of Biochemistry and Biophysics. (2007), 44, 176-178. Communicated: 1.) Insight into the proteome of Enterobacter hormaechei AAB8, a psychrotolerant strain isolated from Indian Himalayan Soil. K. Premalatha, Deep C. Suyal, Nidarshana Chaturvedi, Brijesh S. Sisodia, Ajit K. Shasany , Rajendra P. Patel, Shachi Gahoi, Soma S. Marla and R. Goel. Communicated to Canadian Journal of Microbiology, (2013) . SEMINARS/SYMPOSIA Chaturvedi N, Mann HS, Shasany AK, 2006. Opium Poppy: Metabolic Engineering of Alkaloid biosynthetic pathway. Presented at the National Interactive Meet (NIM-2006) on “Scope and opportunities in research and business of medicinal and aromatic plants for national and international markets”, organized at CIMAP, Lucknow during 27th-28th October, 2006. Chaturvedi N, Kumar Santosh, Shasany AK, 2006. Heterologous expression of therapeutic proteins. Presented at the National at the National Interactive Meet (NIM-2006) on “Scope and opportunities in research and business of medicinal and aromatic plants for national and international markets”, organized at CIMAP, Lucknow during 27th-28th October, 2006 Nidarshana Chaturvedi, Ajit K Shasany, Ashutosh K Shukla and Suman PS Khanuja, 2007.Presented at the third Symposium on Current Advances in Molecular Biochemistry: Applications in Health, Environment and Agriculture on “Comparative EST-based analysis of high and low alkaloid containing genotypes of Papaver somniferum “organized at the Department of Biochemistry, University of Lucknow, and Lucknow during 28th-30th December, 2007. Shukla AK, Birtharey V, Chaturvedi N, Lal RK, Shasany AK, Khanuja SPS, 2008. Molecular tagging of specific chemotypes of Papaver somniferum using functional genomics approach towards development of non-narcotic poppy. Presented at the Bangalore Bio 2008 Poster Session – Walkway of Discovery at Bangalore International Exhibition Centre, Bangalore during 24th - 26th April, 2008. Nidarshana Chaturvedi , Ashutosh K Shukla , Raj K. Lal, Anil K Gupta, Om P Dhawan, Madan M Gupta, Karuna Shanker, Ajit K Shasany, 2008. Isoquinoline alkaloid biosynthesis-related gene expression profiling in different genotypes of Papaver somniferum. Presented at the National Interactive Meet (NIM-2008) on “Scope and opportunities in research and business of medicinal and aromatic plants Meeting the global competition and quality standards”. organized at CIMAP, Lucknow during 29th-30th November, 2008. Attended International Symposium “AROMED” with theme International Symposium on current status and opportunities in Aromatic and Medicinal Plants from Feb. 21-Feb.24 2010 at CIMAP, Lucknow. Participated and won IInd prize in poster presentation in “First International Symposium on Papaver” from Feb 7-Feb-11 2011. Attended SBC 2011, from Nov.13- Nov.15. Awarded Carl Storm International Diversity Fellowship for presenting paper in Gordon Research Seminar and Gordon Research Conference on Plant Metabolic Engineering held at New Hampshire, USA from July 23 - July 29, 2011. EDUCATIONAL BACKGROUND PhD in Biochemistry Title - Molecular studies on biosynthesis of morphinan alkaloids in Papaver somniferum. Biochemistry Division, Lucknow University, Lucknow. Supervisors: Prof. U.N.Dwivedi (Department of Biochemistry, University of Lucknow, Lucknow and Dr. Ajit K. Shasany, Head, International Scientific Collaboration Division and Senior Principal Scientist, Biotechnology,CSIR-CIMAP, Lucknow (UP) M.Sc. in Biotechnology North Maharashtra University, Jalgaon, Maharashtra Post Graduation Year: 2004 Class & Percentage: 1st - Class with 79.20% B.Sc. In Zoology, Chemistry, Industrial Microbiology Rajasthan University, Jaipur, Rajasthan Graduation Year: 2002 Class & Percentage: 1st -Class with 74.30% Intermediate with Physics , Chemistry, Biology CBSE Board Year: 1999 Class & Percentage: 1st -Class with 79.20% High School with English, Hindi, Science, Social Science, Mathematics Rajasthan Board Year: 1997 Class & Percentage: 1st -Class with 83.64% ACHIEVEMENTS Awarded “Gargi Award” for securing more than 75% in X Rajasthan board. Awarded Gold Medal for standing Ist in university M.Sc. Examination, year 2004. Awarded “Bal Hutatma Scholarship” from Maharashtra Chief minister Fund during 2002-2003. ● Awarded CSIR-Senior Research Fellowship from CSIR (India) from 20082011. TRAINING AND WORKSHOP ATTENDED Undergone summer training at Cell and Molecular Biology lab, Delhi University, New Delhi (year 2001). Undergone summer training at National Chemical Laboratory, Pune (year 2003). Undergone training in CSIR sponsored “CIMAP Winter School” organized by CIMAP during Dec.2005. Participated in “Indo-Thai Workshop” from 09-01-06 to 13-01-06, sponsored by DBT, CSIR. TRAINING IMPARTED ● Given Training in CIMAP Winter School (CWS-2007) organized by CIMAP-CSIR on Recent techniques in structural and functional genomics, during 1st – 10th December, 2007. ● Given Training in CIMAP Winter School (CWS-2008) organized by CIMAP-CSIR on Recent techniques in structural and functional genomics, during 1st – 10th December, 2008. ● Training imparted during CIMAP Summer School (CSS-2008) organized by CIMAP-CSIR. TECHNICAL BACKGROUND Molecular biology techniques: 1. DNA isolation from yeast cells as well as plant parts. 2. Designing suitable primers, synthesizing them by automated DNA synthesis. 3. PCR and subsequent cloning. 4. Protein expression. 5. RNA Isolation and c-DNA synthesis. 6. Protein Isolation. 7. cDNA library preparation and screening. 8. Designing and developing over-expression and RNAi constructs. 9. Developing Expressed Sequence Tags (ESTs). 10. Automated synthesis of oligonucleotides on ABI prism 392 DNA/ RNA synthesizer. 11. Automated sequencing on ABI XL 3130Genetic Analyzer sequencer. 12. Subtractive Hybridization library preparation and screening. 13. 5’ Rapid Amplification Of cDNA Ends (5’RACE). 14. Semi quantitative analysis of gene expression by RT-PCR. 15. Quantitative PCR (Real -Time PCR) 16. Liquid Handling System Microbiological techniques : 1. Maintaining and preserving pure cultures. 2. Performing microbiological assays. etc. Chemical techniques: 1. HPLC of plant parts and analysis. 2. HPTLC of plant parts and analysis Bioinformatics: 1. Equipped with basic working knowledge of Microsoft Word, Microsoft Excel,Microsoft PowerPoint, Adobe Photoshop presentations, review of documents and manuscripts electronically. 2. Experience in searching databases, and use of bio-informatic tools like BLAST, CAP3 and Clustal W. 3. Experience in working with the biological databases such as NCBI, EMBL and knowledge of bioinformatics tools such as Blast, sequence manipulation suite, Expasy, NEB cutter, clustal X and ClustalW. Protein techniques: 1. Protein isolation, and precipitation. 2. Estimation and SDS-PAGE. 3. Isoelectric focusing (IEF). 4. 2-D gel electrophoresis. SEQUENCE SUBMITTED Sequence of AAT gene (Indian variant) submitted to database, available in NCBI with accession no. DQ682455. (July 2006) Normalized cDNA library EST sequences submitted with the following accession nos.: 275 Capsule wall ESTs: GR979624-GR979888 and GT735891-GT735900 25 Leaf ESTs: GR979889-GR979913 Subtractive hybridized library EST sequences submitted with the accession nos. from GT571567GT571597. The Platform data for the P. somniferum custom array has been submitted to GEO (NCBI) and available under accession number GPL14625. Full length sequence of Laccase from contrasting genotypes of P.somniferum submitted to database with Genbank Accession numbers JX861092 and JX861093. PERSONAL DETAILS: NAME: NIDARSHANA CHATURVEDI PARASHAR GENDER: FEMALE DATE OF BIRTH: 07, AUGUST, 1981 MARITAL STATUS: Married HUSBAND’S NAME: GAURAV PARASHAR FATHER’S NAME: NARENDRA KUMAR CHATURVEDI MOTHER’S NAME: KAMLESH CHATURVEDI NATIONALITY: INDIAN REFERENCES: DR. AJIT K. SHASANY DR. UPENDRA N. DWIVEDI Head, International Scientific Collaboration Division, Professor, Dept. of Biochemistry Senior Principal Scientist, Biotechnology, University of Lucknow, CSIR-CIMAP, Lucknow (UP) Lucknow ak.shasany@cimap.res.in upendradwivedi@hotmail.com DR. ASHUTOSH K. SHUKLA Scientist, Biotechnology Division, CSIR-CIMAP, Lucknow (UP) ashupov@yahoo.com I here by declare that all information that I enclosed here is true and brief as per my knowledge. DATE: 22-08-2014 PLACE: Chandigarh