Cellular Respiration Laboratory
PSI Biology
Name____________________________________
Problem
How does the food source affect the rate the cellular respiration in yeast?
Safety
None
Materials
2 plastic bottles (16.9oz)
15g of the any 2 of the following carbohydrates: sucrose, glucose, lactose, and starch
2 balloons
2 packets of active dry yeast
Hot plate
Balance
Beaker with 400mL of H2O
Thermometer
String
Ruler
Procedure
1. Select the two carbohydrates you wish to compare, and predict which of the two will have the
greatest rate of cellular respiration.
2. Heat the water to between 40OC and 45 OC.
3. While waiting for the water to heat, stretch out the balloons by blowing them up repeatedly. Set
them aside.
4. Label the bottles for each carbohydrate. Fill the bottles 1/3 full of water
5. Add each carbohydrate to its respective bottle. Put the lid on the bottles and shake gently to
dissolve the carbohydrate. Remove the lids and allow the water to settle.
6. Add the contents of a whole packet of yeast to each bottle. Quickly place a balloon over the
mouth of each bottle.
7. Check to make sure the balloon is tight over the tops of the bottles. Gently swirl the contents.
Place the bottles in a warm place.
8. At 15 minute intervals record the circumference of each balloon.
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Energy Processing
Data
Carbohydrate #1: _______________________________________
Time (min)
15
30
45
60
Next class
Circumference (cm)
Volume (mL)
Carbohydrate #2: _______________________________________
Time (min)
15
30
45
60
Next class
Circumference (cm)
Volume (mL)
Analysis
1. Graph the rate of cellular respiration for each carbohydrate.
2. Relate the differences in rate of cellular respiration to the structure of each carbohydrate used.
3. Yeast is a facultative anaerobe. What does this mean?
4. What are the products of fermentation? Is there evidence for each product?
5.
If you were asked to set up a control for this experiment, what would you do?
Conclusion
Was your hypothesis correct? Why or why not? What have you learned from this investigation?
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PSI Biology
Energy Processing