Toxicity of n-hexane, ethyl acetate, and methanol fractions of Imperata
cylindrica
St Khaerunnisa*, Mike Widy Hartatik*, Nanik Siti Aminah, Alfinda Novi Kristanti
* Bachelor degree student of Department of Chemistry, Faculty of Science and Technology,
Airlangga University, Kampus C Universitas Airlangga, Jl. Mulyorejo, Surabaya, Indonesia, 60115
Department of Chemistry, Faculty of Science and Technology, Airlangga University,
Kampus C Universitas Airlangga, Jl. Mulyorejo, Surabaya, Indonesia, 60115
E-mail : nanik_sa2000@unair.ac.id
ABSTRACT
The n-hexane, ethyl acetate, and methanol fractions from Imperata cylindrica has been done
toxicity test with BSLT (Brine-Shrimp Lethality Test) using Artemia salina Leach. Each fraction by
determined 7 concentration, which were 15,625 ppm, 31,25 ppm, 62,5 ppm, 125 ppm, 250 ppm,
500 ppm, and 1000 ppm. Each treatment was replied three times. The result of test was known that
n-hexane extract has highest toxicity with LC50 582,18 ppm. Besides, methanol extract have LC50
554,26 ppm and ethyl acetate extract have LC50 172,18 ppm.
Key words
: toxicity, Imperata cylindrica, Artemia salina Leach