M.Phil MICROBIOLOGY CURRICULUM
AND
LOG BOOK
DEPARTMENT OF MICROBIOLOGY
Mymensingh Medical College, Mymensingh
July 2012
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STUDENT’S PROFILE
Class roll no:
University registration no:
Session: July’200
Name
: .............................................
Father’s Name : .............................................
Mother’s Name : .............................................
BMDC registration no:......................................
Date of Birth
: .............................................
Nationality
: .............................................
Address:
Present with telephone:
Permanent with telephone:
Academic records:
Degree
SSC
Name of the Institute
Year
Division
HSC
MBBS
Service records:
Name of the Office
Head of Microbiology
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Duration
Designation
Govt./Non-Govt.
OVERVIEW OF THE COURESE
Name of the curse
Duration of the course
: Master of Philosophy, Microbiology
:02(Two) years
Comprises Part-I, II (each 6 months) & Part-III (One
year)
Commencement of the course: 1st July of each year
Part-I (Six months):
Paper-I (Biostatistics, Biophysics & Basic Chemistry)
Paper-II (General Bacteriology & Systemic Bacteriology)
Paper-III (Parasitology & Mycology)
Part-II (Six months):
Paper-IV (Virology & Water bacteriology)
Paper-V (Basic & Applied Immunology)
Paper-VI (Biochemistry/ Physiology)
Part-III (One year):
Thesis & Thesis defense
Comprehensive Viva
Examination format
Each paper of Part-I & II
Written
Practical
Oral
Part-III
Thesis & Thesis defense
Comprehensive Viva
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Total Marks
100
50
50
Pass Marks
60
30
30
200
100
120
60
M.Phil (Microbiology) Syllabus
PART-I:
Paper –I (Biostatistics, Biophysics & Basic chemistry)
Biostatistics
1. Introduction to research, research strategies and design
2. Concept regarding major categories of medical research: a) experimental studies b)
descriptive epidemiological studies and c) clinical trials (aspects more relate to basic
sciences research shall be emphasized)
3. Relevant methodological details of collection and interpretation of data a) sampling
techniques and sample size b) questionnaire design c) preparation of data sheet d) bias
and confounding e) basic risk measurements
4. Tests of significance of differences, association and causation
5. Ethical aspects of medical research
6. Preparation of a research proposal (protocol)
7. Writing up of thesis-details of every aspect (from title to appendices) of the thesis
including presentation of results (descriptive statistics) in tables, graphs, charts,
photographs, photomicrographs, etc.
8. Writing a research paper
9. Presentation of a research paper: a) oral presentation b) poster presentation
Teaching methodology
1. Basics of learning and teaching, curriculum planning and quality assurance in the
Institution.
2. Teaching methods: a) basic planning b) giving lectures c) small group teaching d)
taking practical classes.
3. Teaching resources (materials): a) audio-visual aids and their uses.
4. Assessment of student performance: a) principles of assessment b) planning of written
examinations( including setting of various types of written questions and their
comparisons) c) planning of oral and practical examinations (including designing
OSPE stations)
NB: Teaching assignments shall be given to the students and shall be evaluated by the
teachers.
Paper – II
General Bacteriology
Theory:
Bacterial Structure, Nomenclature, Growth and Nutrition, Genetic and Metabolism.
Classification, Media and Staining. Sterilization & Disinfection,
Host parasite Relationship, Genetics, Normal floras & Opportunistic Bacteria,
Bacterial Pathogenesis,Antimicrobial agents and Drug resistance, Milk, food & Water
Bacteriology. Laboratory design and Management and Laboratory safety, Safe disposal of
laboratory products.
Practical:
Laboratory safety, Quality assurance, Safe disposal of laboratory products,
Specimen collection and transportation, Laboratory procedures, Record keeping,
Preparation of Media, Preparation of Staining material, Staining,
Microscopy of Stained smear, Preservation of culture and other sample, Preparation of
Antimicrobial disc, Practice of sterilization and disinfection.
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Systemic Bacteriology
Theory:
Cocci ( Staphylococcous, Streptococcous, Neisseria), Haemophilus, Corynebacteria,
Mycobacteria, Enterobacteriaceae, Vibrioniaceae, Pseudomoniaeae, Plesiomonas
Aeromonas, Listeria, Erysipelothrix, Actinomyces, Acinatobacter, Nocardia, Yersinia,
Pasturella, Bordetella, Francisella, Brucella, Anaerobic (Non sporing) bacteria, Sporebearing bacteria,Helicobacter, Campylobacter, Spirocheate, Campylobacter, Rickettisia,
Chlamydia, Mycoplasma, Legionellae, Gardenella, Actinobacillum. Outbreak
investigation: Sample transportation, Isolation, Identification and reporting, Diarrheal
pathogens
Practical:
1.
1.
2.
3.
4.
Isolation and Identification of unknown Bacteria
Serological procedures in Bacteriology
Biochemical procedures in Bacteriology
Antimicrobial susceptibility
Performance testing and IQC
Paper –III (Parasitology & Mycology)
Parasitology
Theory:
Introduction, Classification of Parasite
Protozoology: Amoebae, Flagellates, Ciliata, Sporozoa
Helminthology: Cystode, Trematode, Nematode
Practical:
1.
2.
3.
4.
5.
Examination of urine
Examination of Stool
Examination of Blood
Examination of Bone marrow
Exam of Aspirate : Liver
Spleen
Lymphnode
6. Examination of adult parasite
Mycology
Theory:
Structure, Classification, Anti fungal drugs
M.furfur, Dermatophytes, Candida.
Deep/Systemic fungus
Opportunistic fungus
Practical
1) Specimen collection
2) Microscopy (Wet film and Stained microscopy)
3) Culture and Serology
4) Reporting
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PART-II
Paper-IV (Virology)
Theory:
General Virology:
Nomenclature, Properties and structure of virus, Viral Pathogenesis, Antiviral Agents, Viral
vaccine
Systemic virology:
Herpes viruses (H. simplex, H. Zooster, Cyto-megalo Virus, Epstein Bar Virus, HSV- 6)
Hepatitis viruses, Virus Associated with diarrhea, Rota virus, Corona virus, Adeno virus
Papilloma virus, Respiratory viruses (Influenza and Para influenza virueses, Respiratory Synsytial
Virus, Adeno virus, Rhino virus) Corona viruses, Measles, Rubella, Mumps
Entero viruses (Polio virus), Pox virus, Viral Hemorrhage fever, Rhabdo virus, Dengue Virus,
Retro viruses, HTLVs, HIVs, Prion disease, Oncogenic viruses, Viral oncogenesis. Milk, Food
and Water bacteriology
Practical:
1) Specimen collection and transport
2) Laboratory safety
3) Laboratory diagnosis of viral diseases
4) Tissue culture and other laboratory Techniques
5) Serological method in virology
6) Testing water/Milk
Paper –V (Basic & Applied Immunology)
Theory:
Structure & organization of Immune system, Cells of Immune system. Antigen, Immunogen,
Hapten. Mediators (Complements, Cytokines and Immunoglobulin), MHC,
Immunity and Immune response. Tolerance, Hypersensitivity, Transplantation Rejection, Auto
immunity, Tumor immunology
Immunity against Bacteria,Virus, Parasite, Fungus, Immunodeficiency, Autoimmune
diseases,Vaccines.
Practical:
Haemolysin preparation, Titration ( Complement, Haemolysin), CFT, Agglutination,
Precipitation, IFAT, ELISA, Electrophoresis, Chromatography, PCR, Western Blot,
Chemiluminescence, Cell separation(Flow cytometry), HLA typing.
Paper VI (Biochemistry/ Pathology)
FINAL PART:
A. Thesis & Thesis defense
B. Comprehensive VIVA
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Comprehensive VIVA will cover all areas of Bacteriology, Parasitology and Mycology,
Immunology & Virology.
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Bacteriology Pactrical
Duration
in Week
One
Section
Two




one



One


Two

Two





Two
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
Orientation in the department and
Reception: Patient dealing, sample
collection, processing, recording &
reporting system
Aseptic practices in laboratory and
safety precautions
Collection/transport of specimens
for microbiological investigations
Sterilization Section, Lab safety,
waste disposal
Introduction to BSL -1& 2
Operation of autoclave, hot air oven,
distillation plant, filter, Refrigerated
centrifuge,
Microwave
oven,
Micropipette, Water bath, Heating
block
Microscopy (Light, DGI, Phase
contrast, Imm FI Microscopy:
Maintenance)
Preparation of stains viz. Gram,
Ziehl Neelsen , Albert’s, capsules,
spores, (ZN) etc.
Preparation,
examination
&
interpretation of direct smears from
clinical specimens ( stool, urine,
Pus, HVS, Sputum, U/S, CSF)
Introduction to Plating of clinical
specimens on media for isolation,
purification,
identification
and
quantitation purposes.
Preparation of media like Nutrient
Broth/ agar, Blood Agar, Macconkey agar,
Robertson’s cooked meat broth,
Lowenstein Jhensen
. Preparation of reagents -oxidase,
Kovac Sugars, Kligler iron agar, etc.
Quality control of media, reagents
etc.
Bleeding techniques of sheep and
preparation of BA and Chocolate
agar media.
Study of Colony Morphology Of
Common Bacteria- Staphyloccus,
Strepcococcus,
Esch
Coli,
Date
Level of
Participation
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
Teachers
comment
Duration
in Week
One
Section


Date
Salmonella, Shigella, Pseudomonas,
Proteus, Klebsiella, Enterococci,
Campylobacter,
Mycobacterium
(Using control strains)
Tests for Motility, hanging drop,
DGI, Soft agar, catalase, oxidase,
Coagulase, (slide & tube) Sugar
fermentation, TSI, Citrate, Indole,
Hippurate etc
Special culture technique including
anaerobiosis, Microaerophilic etc
Identification Of Bacteria Of
Medica1 Importance Upto Species
Level including serotyping.
Two

Two

One

Two

One

One


Disposal of contaminated materials
like cultures
Disposal of infectious waste
Maintenance & preservation of
bacterial cultures
Two

Test of tap water for bacteria and
Plating of clinical specimens on
media for isolation, purification,
identification
and
quantitation
purposes.
Quantitative analysis of urine by
pour plate method and semi
quantitative analysis by standard
loop tests for finding significant
bacteriuria
Culture of Blood, Sputum, CSF,
Stool etc.
colony count
Two

Efficacy of sterility by autoclaving ,
Hot air oven and filtration
Two

Bacteriological study
of Mobiles,
key boards, table top.

Introduction
techniques-
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Level of
Participation
to
Molecular
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
Teachers
comment
Duration
in Week
Section

Date
Level of
Participation
Teachers
comment
Date
Level of
Participation
Teachers
comment
DNA/ RNA extraction from clinical
specimen, Bacterial colony

Different type of Electrophoresis for
detection DNA/RNA

Amplification of DNA

Documentation of amplicon.
Parasitology
Duration
in Week
Two
Section




one



One
Egg counting techniques for
helminths by Mcmaster technique
Preservation of parasites-mounting,
fixing, staining etc.

Examination of blood for protozoa
and helminths by wet mount, thick
and thin stained smears
Examination
of
blood
for
microfilariae
including
concentration techniques
Culture media Leishmania donovani
in NNN medium
Culture of Helminths by Haradimori
technique
Preparation of stains- Giemsa,
Leishman, Methylene blue, Fields
stain, Iron hematoxylene, etc



A
B
C
Aspirate/Biopsy from Bone marrow,
/liver, spleen, L.node, , skin
Examination of faeces for parasite
ova and cysts etc. by direct
Microscopy
and
concentration
methods (salt floatation and formolether methods)



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Collection and transport of specimens
for diagnosis of parasitic diseases
Stool- for Protozoa and Helminths
Blood for MP, Filaria, Kala-azar,
Trypanos,oa,
A
B
C
Mycology
Duration
in Week
Two
Section


one





One




Date
Level of
Participation
Teachers
comment
A
B
C
Collection and transport of specimens
for diagnosis of Fungal diseases
Collection technique for dermatophytes
from Skin, nail and Hair
Processing
of
samples
for
microscopy and culture
o Direct examination of specimens
by KOH, Gram’s, Acid fast,
Giemsa, Lactophenol cotton blue &
special fungal stains
Isolation and identification of
medically important fungi &
common laboratory contaminants
Comparison of SDA and Special
fungal media like Dermatophyte test
media alike
Germ tube test for Candida
Special fungal culture techniques
e.g. Plates culture, Slide culture
Special techniques like hair baiting,
hair perforation, paraffin baiting and
A
B
C

Virology
Duration
in Week
one
Two
Section





Preparation of clinical specimens for
isolation of viruses
Collection & transport of specimens
Serological tests -ELISA for
HBsAg, HBsAB, HBeAg, HCV,
HEV
ICT for HIV, HCV , HEV etc
Molecular techniques in virology
One

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Polyacrylamide Gel electrophoresis
for Rota virus
Date
Level of
Participation
A
B
C
A
B
C
Teachers
comment
Duration
in Week
Section



Date
Level of
Participation
Teachers
comment
Date
Level of
Participation
Teachers
comment
Introduction to cell/ Tissue culture
Preparation of glassware for tissue
cultures (washing, sterilisation).
Preparation of buffers like PBS,
Hank’s, TBE
Water Bacteriology
Duration
in Week
one
Section
 Examination of Water –
o Sampling – Tap water, River
water, Tube well water
o Counting indicator bacteria-
A
B
C
Fecal Esch coli
Two
One
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
o Filtration method
o Multiple tube - MPN
Examination of Milk –
o Methylene blue test
o Phosphatase test

Food Bacterilogy
A
B
C
Book List
Bacteriology
1.
Medical Microbiology,LANGE
2.
Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases
3.
Medical Microbiology Patrick. M. Murry
4.
District Laboratory Practice in Tropical Countries; Part 1 and Part II; Monica Cd
Cheesbrough, Cambridge university press. Reprint 2007
5.
Mackie & McCartney ; Practical Medical Microbiology, Fourteenth Edition,
Churcill Livingstone
Immunology
1.
Medical Immunology, LANGE
2.
Immunology ; Richad A. Goldsby, Janis Kuby
3.
Roitt’s Essential Immunology
Parasitology
1.
K. D. Chatterjee
2.
Medical Parasitology basic and clinical.
Mycology
Medically Important Fungi, 4th Edition, Davise H Larone, ASM press,
Washington DC
Human Mycology by Dr. Ahmed Abu Saleh
1.
2.
B. ASSIGNMENT
Date Topic of Assignment
1
2
3
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Duration
Date of
Submission
Score
Signature Coordinator
C. Journal club Registrar
No.
1
Topics
Date
Coordinator
Date
Coordinator
2
3
D. Seminar Registrar
No.
1
Topics
2
This is to certify that Dr.............................................................. having the
BSMMU egistration no................................ are correct and eligible/not eligible to
appear in the Part-I examination.
Signature of the Course Co-ordinator:
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Name of the Institute:
Head
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of
the
department:
B. ASSIGNMENT
Date
Topic of Assignment
Duration
Date of Submission
Score
1
2
3
C. Journal club Registrar
No.
Topics
1
Date
Coordinator
2
3
4
5.
6.
D. Seminar Registrar
No.
1
Topics
2
3
4.
5
6
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Date
Coordinator
Signature
Coordinator
This is to certify that Dr.............................................................. having the Dhaka
University registration no................................ are correct and eligible/not eligible
to appear in the Part-II examination.
Signature of the Course Co-ordinator:
Name of the Institute:
Head of the department:
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Certificate:
Area of activity
Attendance
Performance
Signature
Attention
Laboratory skill
Communication skill
Journal presentation
Seminar presentation
Research skill
Total
Remarks by Head:
Assignments
1. Discovery of vaccine is a mile stone in the Medical science.
2. Discovery of solid media is breakthrough in the filed of bacteriologyDiscuss
3. Common bacterial infections in Bangladesh with their prevalence in BD
and other parts of world.
4. Common parasitic infections in Bangladesh with their prevalence in BD
and other parts of world.
5. Common fungal infections in Bangladesh with their prevalence
prevalence in BD and other parts of world.
6.
7.
You have been called on to prevent outbreaks of staphylococcal scalded skin
syndrome in the nursery of a hospital where an MRSA strain of S. aureus has
been isolated. Explain what this organisms is, your main concerns and what
procedures you would establish to address them
2. Explain the historical world impact made by epidemic typhus, discussing the
disease’s mode of transmission, symptoms and disease progression. Describe
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how this disease was finally brought under control, explaining current methods
of treatment
3. Conduct an on-line search for emerging protozoan pathogens. Identify five of
these species and make notes of the sources of infection and diseases caused
5. All three of the living microbial kingdoms, bacteria, fungi and protozoa coexist on our planet. If a global catastrophe were to occur, and only ONE of
these could be preserved, which would you choose?
6. A flask of water is inoculated in BA media then heated in hot air oven for at
180 0C for 10 min, 30 min, and 60 min and then inoculated in BA and
incubated at 37 0 C? Interpret your findings.
7. A flask of water is inoculated in BA media then heated in Autoclave for 50
min, 10 min, and 30 min and then inoculated in BA and incubated at 37 0 C?
Interpret your findings.
8. Most important in 20th and 21st century in medical microbiology.
9. Isolate from Microbes from mobiles used by officers and staff of
Microbiology, MMC.
10. Re-emergence of tuberculosis worldwide: reasons and approaches for
a solution.
11. Occasional spread of polio despite universal vaccination; reasons and
approaches towards a solution.
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