Biology 111 and 112 Course Overview Exam Note Package 1-1 What is Science? (page 3) The Goal of Science 1) deals only with the natural world 2) to collect and organize information 3) propose explanations that can be tested Science - using evidence to learn about the natural world; a body of knowledge Science begins with observations data - the information gathered from observations quantitative data = numbers qualitative data = descriptive inference - a logical interpretation based on prior knowledge or experience hypothesis - a proposed scientific explanation ***Science is and ongoing process*** 1-2 How Scientist Work (page 8) Spontaneous generation - the idea that life could arise from non-living matter Francesco Redi (1668) (Fig 1-8) Lazzaro Spallanzini (Fig 1-10) Louis Pasteur (1800's) (figure 1-11) Scientific Method: (see page 1062) 1) Ask questions, make observations 2) Gather information 3) Form a hypothesis 4) Set up a controlled experiment Manipulated variable - the variable that is deliberately changed (independent variable) Responding variable is variable that is observed ( aka dependent varable) 5)Record and analyze results 6)Draw a conclusion 7)Repeat ***Field studies, models *** Theory - a well-tested explanation that unifies a broad range of observations. NOT ABSOLUTE 1-3 Studying Life (page 16) biology means the study of life Bios = life -logy = study of The 8 Characteristics of Living Things: 1) Cell - smallest unit of life unicellular = single celled multicellular = many celled 2) Reproduction sexual reproduction -DNA from two different parents asexual reproduction - single parent (cloning, budding) 3) Genetic Code - directions for inheritance (DNA) 4) Growth and Development growth = getting larger development = changing shape and structure Differentiation = cells that look different and perform different functions 5) Obtain and use energy metabolism - chemical reactions plants - photosynthesis Animals - eating 6) Response to the Environment stimulus - a signal to which we respond response - a reaction to a stimulus Ex: school bell rings, we move to next class 7) Internal Balance homeostasis -internal conditions remain constant Ex: lizards sun themselves 8) Evolution - Change over time Branches of Biology: Zoologists - animals Botanist - plants Paleontologist - ancient life Cytologist - cells Levels of organization (page 21, figure 1-21) Molecules ' Cells ' Tissues ' Organs ' ' Organ systems ' Organisms 'Population ' Community ' Ecosystem ' Biosphere 1-4 Tools and Procedures Common Measurement System Metric system - decimal system of measurements, units are scaled on multiples of 10 UNIT TOOL Length Meter, Centimeter, Kilometer Ruler, Meter Stick Volume Liter, Milliliter Graduated Cylinder Mass Kilogram, Gram Balance, scale Temperature - The measure of hotness (Celsius) Analyzing data -- Tables, Graphs, Charts, Drawings, Models, etc. Microscopes - produce a magnified image of structures Light Microscope May be Simple or Compound (one lens) or (two or more lenses) **Specimen can remain alive** Electron Microscope SEM - 3-D image TEM - through an image **Specimens cannot be observed while alive** Laboratory Techniques Cell culture - group of cells grown in a nutrient solution from a single original cell Cell fractionation - technique in which cells are broken into pieces and parts are separated How to Use the Microscope PowerPoint on the Microscope (click to advance frames) Types of Microscopes Light Microscope - the models found in most schools, use compound lenses and light to magnify objects. The lenses bend o the light, which makes the object beneath them appear closer. Stereoscope - this microscope allows for binocular (two eyes) viewing of larger specimens. (The spinning microscope at the t page is a stereoscope) Scanning Electron Microscope - allow scientists to view a universe too small to be seen with a light microscope. SEMs donÕ light waves; they use electrons (negatively charged electrical particles) to magnify objects up to two million times. (View Image Transmission Electron Microscope - also uses electrons, but instead of scanning the surface (as with SEM's) electrons are through very thin specimens. (View images) Parts of the Microscope Quiz Yourself on Naming the Parts of the Microscope! Magnification Your microscope has 3 magnifications: Scanning, Low and High. Each objective will have written the magnification. In addition the ocular lens (eyepiece) has a magnification. The total magnification is the ocular x objective Magnification Ocular lens Total Magnification Scanning 4x 10x 40x Low Power 10x 10x 100x High Power 40x 10x 400x General Procedures 1. Make sure all backpacks and junk are out of the aisles. 2. Plug your microscope in to the extension cords. Each row of desks uses the same cord. 3. Always start and end with the Scanning Objective. Do not remove slides with the high power objective into place - this will s lens! 4. Always wrap electric cords and cover microscopes before returning them to the cabinet. Microscopes should be stored with the Scanning Objective clicked into place. 5. Always carry microscopes by the arm and set them flat on your desk. Focusing Specimens 1. Always start with the scanning objective. Odds are, you will be able to see something on this setting. Use the Coarse Kn focus, image may be small at this magnification, but you won't be able to find it on the higher powers without this first step. Do stage clips, try moving the slide around until you find something. 2. Once you've focused on Scanning, switch to Low Power. Use the Coase Knob to refocus. Again, if you haven't focused level, you will not be able to move to the next level. 3. Now switch to High Power. (If you have a thick slide, or a slide without a cover, do NOT use the high power objective). At ONLY use the Fine Adjustment Knob to focus specimens. 4. If the specimen is too light or too dark, try adjusting the diaphragm. 5. If you see a line in your viewing field, try twisting the eyepiece, the line should move. That's because its a pointer, and is us pointing out things to your lab partner or teacher. Drawing Specimens 1. Use pencil - you can erase and shade areas 2. All drawings should include clear and proper labels (and be large enough to view details). 3. Drawings should be labeled with the specimen name and magnification. 4. Labels should be written on the outside of the circle. The circle indicates the viewing field as seen through the eyepiece, specimens should be drawn to scale ie..if your specimen takes up the whole viewing field, make sure your drawing reflects that. Making a Wet Mount 1. Gather a thin slice/peice of whatever your specimen is. If your specimen is too thick, then the coverslip will wobble on top of the sample like a see-saw, and you will not be able to view it under High Power. 2. Place ONE drop of water directly over the specimen. If you put too much water, then the coverslip will float on top of the water, making it hard to draw the specimen, because they might actually float away. (Plus too much water is messy) 3. Place the coverslip at a 45 degree angle (approximately) with one edge touching the water drop and then gently let go. Performed correctly the coverslip will perfectly fall over the specimen. How to Stain a Slide 1. Place one drop of stain (iodine, methylene blue..there are many kinds) on the edge of the coverslip. 2. Place the flat edge of a piece of paper towel on the opposite side of the coverlip. The paper towel will draw the water out from under the coverslip, and the cohesion of water will draw the stain under the slide. 3. As soon as the stain has covered the area containing the specimen, you are finished. The stain does not need to be under the entire coverslip. If the stain does not cover as needed, get a new piece of paper towel and add more stain until it does. 4. Be sure to wipe off the excess stain with a paper towel. Cleanup 1. Store microscopes with the scanning objective in place. 2. Wrap cords and cover microscopes. 3. Wash slides in the sinks and dry them, placing them back in the slide boxes to be used later. 4. Throw coverslips away. Troubleshooting Occasionally you may have trouble with working your microscope. Here are some common problems and solutions. 1. Image is too dark! Adjust the diaphragm, make sure your light is on. 2. There's a spot in my viewing field, even when I move the slide the spot stays in the same place! Your lens is dirty. Use lens paper, and only lens paper to carefully clean the objective and ocular lens. The ocular lens can be removed to clean the inside. 3. I can't see anything under high power! Remember the steps, if you can't focus under scanning and then low power, you won't be able to focus anything under high power. 4. Only half of my viewing field is lit, it looks like there's a half-moon in there! You probably don't have your objective fully clicked into place. The Cell Overview Early Contributions Robert Hooke - The first person to see cells, he was looking at cork and noted that he saw "a great many boxes. (1665) Anton van Leeuwenhock - Observed living cells in pond water, which he called "animalcules" (1673) Theodore Schwann - zoologist who observed that the tissues of animals had cells (1839) Mattias Schleiden - botonist, observed that the tissues of plants contained cells ( 1845) Rudolf Virchow - also reported that every living thing is made of up vital units, known as cells. He also predicted that cells come from other cells. (1850 ) The Cell Theory 1. Every living organism is made of one or more cellss. 2. The cell is the basic unit of structure and function. It is the smallest unit that can perform life functions. 3. All cells arise from pre-existing cells. *Why is the Cell Theory called a Theory and not a Fact? Cell Features Ribosomes - make protein for use by the organism Cytoplasm - jelly-like goo on the inside of the cell DNA - genetic material Cytoskeleton - the internal framework of the cell Cell membrane - outer boundary of the cell, some stuff can cross the cell membrane. Types of Cells: Prokaryotic Cells Prokaryotes are very simple cells, probably first to inhabit the earth. Prokaryotic cells do not contain a membrane bound nucleus. Bacteria are prokaryotes. DNA of bacteria is circular. The word "prokaryote" means "before the nucleus" Other features found in some bacteria: Flagella - used for movement Pilus - small hairlike structures used for attaching to other cells Capsule - tough outer layer that protects bacteria, often associated with harmful bacteria Eukaryotic Cells Eukaryotic cells are more advanced cells. These cells are found in plants, animals, and protists (sma unicellular "animalcules"). The eukaryotic cell is composed of 4 main parts: cell membrane - outer boundary of the cell cytoplasm - jelly-like fluid interior of the cell nucleus - the "control center" of the cell, contains the cell's DNA (chromosomes) organelles - "little organs" that carry out cell functions Organelle Cell Part Function Mitochondria Energy center or "powerhouse" of the cell. Turns food into useable energy (ATP) Ribosomes Make protein Golgi Apparatus Processes, packages and secretes proteins. Like a factory. Lysosome Contains digestive enzymes, breaks things down, "suicide sac" Endoplasmic Reticulum Smooth ER - no ribosomes Rough ER - ribosomes Transport, "intracellular highway". Ribosomes are positioned along the rough ER, protein made by the ribosomes enter the ER for transport. Nucleolus Located inside the nucleus, makes ribosomes Vacuole Stores water or other substances, plant cells contain a large central vacuole. Chloroplast Uses sunlight to create food, photosynthesis (only found in plant cells) Cell Wall Provides additional support (plant and bacteria cells) Part of the cytoskeleton, function in support Microtubules Also make up cilia and flagella (cell movement) Protein Production: Ribosomes make protein and send them through the ER to the golgi apparatus, the GA then processes the proteins, exports it to where the protein is needed. Animal Cell Plant Cell ORGANELLES WITH DNA The Mitochondria and Chloroplasts have their own DNA ENDOSYMBIOSIS THEORY - eukaryotic cells evolved from the engulfing of bacteria cells, thus creating additional cell parts http://www.wiley.com/legacy/college/boyer/0470003790/animations/cell_structure/cell_structure.htm http://biologycorner.com/worksheets/dragonfly/ch7_review.html http://school.discoveryeducation.com/puzzles6/muskopf/html/44181xlaxk.html CELL MEMBRANE Function: to regulate what comes into the cell and what goes out Composed of a double layer of phospholipids and proteins Diffusion and Osmosis Diffusion - the process by which molecules spread from areas of high concentratiion, to areas of low concentration. When the molecules are even throughout a space - it is called EQUILIBRIUM Concentration gradient - a difference between concentrations in a space. OSMOSIS Watch this animation of water molecules moving across a selectively permeable membrane. Water molecules are the small blue shapes, and the solute is the green. The solute is more concentrated on the right side to start with, which causes molecules to move across the membrane toward the left until equilibrium is reached. Start Animation Selectively Permeable - membranes that allow some things through, the cell membrane is selectively permeable, water and oxygen move freely across the cell's membrane, by diffusion Osmosis - the diffusion of water (across a membrane) Water will move in the direction where there is a high concentration of solute (and hence a lower concentration of water. A simple rule to remember is: Salt is a solute, when it is concentrated inside or outside the cell, it will draw the water in its direction. This is also why you get thirsty after eating something salty. Type of Solutions If the concentration of solute (salt) is equal on both sides, the water will move back in forth but it won't have any result on the overall amount of water on either side. "ISO" means the same The word "HYPO" means less, in this case there are less solute (salt) molecules outside the cell, since salt sucks, water will move into the cell. The cell will gain water and grow larger. In plant cells, the central vacuoles will fill and the plant becomes stiff and rigid, the cell wall keeps the plant from bursting In animal cells, the cell may be in danger of bursting, organelles called CONTRACTILE VACUOLES will pump water out of the cell to prevent this. The word "HYPER" means more, in this case there are more solute (salt) molecules outside the cell, which causes the water to be sucked in that direction. In plant cells, the central vacuole loses water and the cells shrink, causing wilting. In animal cells, the cells also shrink. In both cases, the cell may die. This is why it is dangerous to drink sea water - its a myth that drinking sea water will cause you to go insane, but people marooned at sea will speed up dehydration (and death) by drinking sea water. This is also why "salting fields" was a common tactic during war, it would kill the crops in the field, thus causing food shortages. Diffusion and Osmosis are both types of PASSIVE TRANSPORT - that is, no energy is required for the molecules to move into or out of the cell. Sometimes, large molecules cannot cross the plasma membrane, and are "helped" across by carrier proteins - this process is called facilitated diffusion. ACTIVE TRANSPORT Active Transport - When cells must move materials in an opposite direction - against a concentration gradient. It requires Energy. Proteins or Pumps are found in the cell membrane transport molecules across the membrane. Molecular Transport - Proteins are used to move small molecules such as calcium, potassium, and sodium ions across the membrane Endocytosis - cell takes in large particles by engulfing them Phagocytosis - "cell eating" - extensions off cytoplasm surround a particle and package it within a food vacuole and then the cell engulfs it. Ex. Amoebas use this process. Pinocytosis - the process of taking up liquid from the surrounding environment. Tiny pockets form along the membrane, fill with liquid, and pinch off. Exocytosis - cell gets rid of particles, opposite of endocytosis http://biologycorner.com/bio1/cw_celltransport.html http://biologycorner.com/bio1/qz_diffusion.html The Plasma Membrane --the fluid mosaic model (S.J Singer) -- semi-permeable --fluid portion is a double layer of phospholipids, called the phospholipid bilayer Jobs of the cell membrane: Isolate the cytoplasm from the external environment Regulate the exchange of substances Communicate with other cells Identification Phospholipid bilayer Phospholipids contain a hydrophilic head and a nonpolar hydrophobic tail Hydrogen bonds form between the phospholipid "heads" and the watery environment inside and outside of the cell Hydrophobic interactions force the "tails" to face inward Phospholipids are not bonded to each other, which makes the double layer fluid Cholesterol embedded in the membrane makes it stronger and less fluid Proteins embedded in membrane serve different functions 1. Channel Proteins - form small openings for molecules to difuse through 2. Carrier Proteins- binding site on protein surface "grabs" certain molecules and pulls them into the cell 3. Receptor Proteins - molecular triggers that set off cell responses (such as release of hormones or opening of channel proteins) 4. Cell Recognition Proteins - ID tags, to idenitfy cells to the body's immune system 5. Enzymatic Proteins - carry out metabolic reactions Transport Across Membrane Passive Transport: Simple Diffusion - water, oxygen and other molecules move from areas of high concentration to areas of low concentration, down a concentration gradient Facilitation Diffusion - diffusion that is assisted by proteins (channel or carrier proteins) Osmosis - diffusion of water. Salt Sucks Osmosis affects the turgidity of cells, different solution can affect the cells internal water amounts Contractiles Vacuoles are found in freshwater microorganisms - they pump out excess water Turgor pressure occurs in plants cells as their central vacuoles fill with water. Active Transport - involves moving molecules "uphill" against the concentration gradient, which requires energy Endocytosis - taking substances into the cell (pinocytosis for water, phagocytosis for solids) Exocytosis - pushing substances out of the cell, such as the removal of waste Taxonomy - the science of classifying Common Names spider monkey sea monkey sea horse gray wolf firefly crayfish mud puppy horned toad ringworm black bear jellyfish *Common names can be confusing and names can vary by region. Why Classify? About 1.5 million species named 2-100 million species yet to be discovered Taxonomy =science of classifying organisms --groups similar organisms together --assigns each a name Naming Organisms: Organisms have common & scientific name -all organisms have only 1 scientific name -usually Latin or Greek -developed by Carolus Linnaeus This two-word naming system is called Binomial Nomenclature -written in italics (or underlined) -1st word is Capitalized --Genus -2nd word is lowercase ---species Examples: Felis concolor, Ursus arctos, Homo sapiens, Panthera leo , Panthera tigris The scientific name is always italicized or underlined. Genus is capitalized. Species is not. Scientific names can be abbreviated by using the capital letter of the genus and a period: Example. P. leo (lion) Members of the same genus are closely related. Only members of the same species can interbreed (under natural conditions) Some hybrids do occur under unnatural conditions: Ligers are crosses between tigers and lions. Linneaus - devised the current system of classification, which uses the following schema Kingdom Phylum/Division Class Order Family Genus Species Human Cougar Tiger Pintail Duck Kingdom Animalia Animalia Animalia Animalia Phylum/Division Chordata Chordata Chordata Chordata Class Mammalia Mammalia Mammalia Aves Order Primate Carnivora Carnivora Anseriformes Family Homindae Felidae Felidae Anatidae Genus Homo Felis Panthera Anas Species sapiens concolor tigris acuta 18-2 Modern Evolutionary Classification Linnaeus grouped species mainly on visible similarities & differences Today, taxonomists group organisms into categories that represent lines of evolutionary descent (phylogeny) Evolutionary relationships among a group of organisms can be shown on a cladogram (see 18-7 p. 452) Similarities in DNA and RNA DNA & RNA is similar across all life forms Genes of many organisms show important similarities at the molecular level DNA shows evolutionary relationships & helps classify organisms The Six Kingdoms and Domains number of Cells energy cell type examples archaebacteria unicellular some autotrophic, most chemotrophic prokaryote "extremophiles" eubacteria unicellular autotrophic and heterotrophic prokaryote bacteria, E. coli fungae most multicellular heterotrophic eukaryote mushrooms, yeast plantae multicellular autotrophic eukaryote trees, grass animalia multicellular heterotrophic eukaryote humans, insects, worms protista most unicellular heterotrophic or autotrophic eukaryote ameba, paramecium, algae Using Dichotomous Keys A dichotomous key is a written set of choices that leads to the name of an organism. Scientists use these to identify unknown organisms. Consider the following animals. They are all related, but each is a separate species. Use the dichotomous key below to determine the species of each. 1. Has green colored body ......go to 2 Has purple colored body ..... go to 4 2. Has 4 legs .....go to 3 Has 8 legs .......... Deerus octagis 3. Has a tail ........ Deerus pestis Does not have a tail ..... Deerus magnus 4. Has a pointy hump ...... Deerus humpis Does not have a pointy hump.....go to 5 5. Has ears .........Deerus purplinis Does not have ears ......Deerus deafus Answers: A. Deerus magnus B. Deerus pestis C. Deerus octagis D. Deerus purplinis E. Deerus deafus F. Deerus humpis *note that all of these organisms are in the same genus. http://biologycorner.com/worksheets/dragonfly/ch18_review.html Viruses Properties of viruses: Virus Structure no membranes, cytoplasm, ribosomes, or other cellular components they cannot move or grow they can only reproduce inside a host cell they consist of 2 major parts - a protein coat, and hereditary material (DNA or RNA) they are extremely tiny, much smaller than a cell and only visible with advanced electron microscopes Parasitic Nature Obligate intracellular parasites Specific to their hosts (human, dog, some can cross species) They can only attack specific cells , the common cold is a virus that specifically attacks cells of the respiratory track (hence the coughing and sneezing and sniffling). HIV virus specifically attacks white blood cells Viral Reproduction Lytic cycle = reproduction occurs, cells burst Lysogenic cycle = reproduction does not immediately occur (dormancy) Virulent = viruses that undergo both cycles Viral Replication (see page 404-405) Viruses multiply, or replicate using their own genetic material and the host cell's machinery to create more viruses. Viruses cannot reproduce on their own, and must infect a host cell in order to create more viruses. 1. Attachment 2. Penetration - the virus is engulfed by the cell (Cell can enter Lysogenic or Lytic Cycle) 3. Biosynthesis - viral components are made (protein coat, capsid, DNA/RNA) 4. Maturation - assembly of viral components 5. Release - viruses leave host cell to infect new cells (often destroys host) The following image outlines a typical cycle of a bacteriophage called Lambda: Bacteriophage - viruses that infect bacteria. Animation of a bacteriophage Retroviruses -- RNA viruses that have a DNA stage: Human Immunodefiency Virus - causes AIDS Retrovirus (RNA inside a protein coat) Reverse Transcriptase makes DNA from the virus RNA DNA inserts into host DNA Proteins are assembled from the DNA code Viruses assembled from the proteins Viruses released from the cell Animated Retrovirus See Animation of HIV infection | HHMI Animation (browse for Viral Infection) | HIV (Learner.org) | Hopkins Institute Assignments: HIV Coloring Emerging Viruses illnesses not previously known AIDS, West Nile Virus, SARS, Ebola, Bird Flu Could be mutations of known viruses Could be viruses exposed when knew areas were developed Could have jumped species Related to Viruses Viroids - even smaller than viruses, consist of RNA strands that lack a protein coat Prions - "rogue protein", believed to be the cause of Mad Cow Disease, also may causes Kuru in cannibal tribes Viral Images -- Electron Microscopes Protists Protists belong to the Kingdom Protista, which include mostly unicellular organisms that do not fit into the other kingdoms. Characteristics of Protists mostly unicellular, some are multicellular (algae) can be heterotrophic or autotrophic most live in water (though some live in moist soil or even the human body) ALL are eukaryotic (have a nucleus) A protist is any organism that is not a plant, animal or fungus Protista = the very first Classification of Protists how they obtain nutrition how they move Animallike Protists - also called protozoa (means "first animal") - heterotrophs Plantlike Protists - also called algae - autotrophs Funguslike Protists - heterotrophs, decomposers, external digestion Animallike Protists: Protozoans . Four Phyla of Animallike Protists Classified by how they move Zooflagellates - flagella Sarcodines - extensions of cytoplasm (pseudopodia) Ciliates - cilia Sporozoans - do not move Zooflagellates move using one or two flagella absorb food across membrane Leishmania Sarcodines Ameba (See Ameba Coloring Sheet) moves using pseudopodia ( "false feet" ), which are like extensions of the cytoplasm --ameboid movement ingests food by surrounding and engulfing food (endocytosis) , creating a food vacuole reproducing by binary fission (mitosis) contractile vacuole - removes excess water can cause amebic dysentery in humans - diarrhea and stomach upset from drinking contaminated water Other sarcodines: Foraminferans, Heliozoans See Ameba Move Ciliates Paramecium (See Paramecium Coloring Sheet) move using cilia has two nuclei: macronucleus, micronucleus food is gathered through the :mouth pore, moved into a gullet, forms a food vacuole anal pore is used for removing waste contractile vacuole removes excess water exhibits avoidance behavior reproduces asexually (binary fission) or sexually (conjugation) outer membrane -pellicle- is rigid and paramecia are always the same shape, like a shoe See a Paramecium Swim Sporozoans do not move on their own parasitic Malaria is a sporozoan, infects the liver and blood Parasitic Protists Parasite - an organism that lives on or in a host organism and causes harm to that organism Vector - an organism that can carry a parasite, and is responsible for infecting other organisms (host) with that parasite. Vectors themselves are not harmful, but in the battle against human disease, controlling the vector can control the transmission of parasites. Malaria Protist: Plasmodium Vector: Anopholes Mosquito Statistics: According to the World Health Organization, 300-500 million cases of malaria occur each year Malaria results in 1.5-2.7 million deaths per year (much more than AIDS) Most cases occur in Africa and South America Symptoms include fever, headache, vomitting and other flu-like symptoms The protist lives inside the bloodstrea, eventually clogging capillaries and destroying blood cells, which will lead to death if not treated The arrow points to the purplish colored protist (Plasmodium), the pinkish spheres are blood cells Anopheles moquisto taking a blood meal, this is how a human becomes infected with plasmodium and contracts Malaria African Sleeping Sickness (or Trypanosomiasis) Protist: Trypanosoma Vector: Tse Tse Fly Statistics: Occurs mostly in sub-saharan africa Symptoms include fever, headaches, pain in joints -followed by a phase when the parasite infects the central nervous system, causing confusion, lack of coordination, and uncontrolled sleepiness. Without treatment, the host will die For more information, see http://www.who.int/emc/diseases/tryp/trypanodis.html This slide shows a blood smear of a person infected with trypanosoma. The protist is the purplish colored string-like things. They appear string-like due to a flagella. The reddish circles are blood cells. Giardiasis Protist: Giardia Transmission: Drinking contaminated water (usually outdoor streams and other untreated water) Symptoms: Severe diarrhea and vomitting, the protist takes up residence in the digestive tract. B = Protist, Giardia A = flagella Other Protist Parasites Cryptsporidium - this protist was responsible for a major health crisis in detroit when the city's drinking water became contaminated Amebic Dysentery - also known as Montezuma's Revenge, travellers often contract this in other countries (causes diarrhea) Questions for Thought 1. Does the United States have a responsibility toward treating and containing parasitic infections found in other parts of the world? 2. Why is controlling the vector important to control the disease? 3. One of the best ways to prevent many parasitic infections is to have a source of clean water. Why do you think many third world countries have more incidence of parasitic infection that other countries? Plantlike Protists: Unicellular Algae contain chlorophyll and carry out photosynthesis commonly called algae four phyla: euglenophytes, chrysophytes, diatoms, dinoflagellates accessory pigments help absorb light, give algae a variety of colors Euglenophytes Euglena live in water have 2 flagella for movement use chlorplasts for photosynthesis, but can turn into heterotrophs if they are kept in the dark has an eyespot used for sensing light and dark pellicle - like a cell wall, helps maintain their shapes Chrysophytes Diatoms Dinoflagellates yellow-green algae, "golden plants" produce thin cell walls of silicon, main component of glass Often have two flagella luminescent Ecology of Unicellular Algae make up the base of aquatic food chains phytoplankton makes up half of the photosynthesis that occurs on earth (oxygen) can cause Red Tides - algal blooms - which are toxic Plantlike Protists: Red, Brown, Green Algae Green Algae: Phylum Chlorophyta Unicellular green algae, Colonial (volvox), Multicellular (ulva, sea lettuce) Spirogyra live in water, multicellular named after a spiral shaped chloroplast autotrophic Funguslike Protists heterotrophs, decomposers called slime molds and water molds water molds responsible for the Irish Great Potato Famine PROKARYOTES *include bacteria and archaea *singular: bacterium / plural: bacteria PROPERTIES 1. Bacteria are classified into two kingdoms: Eubacteria (true bacteria) and Archaebacteria (Ancient Bacteria). 2. Bacteria are the MOST NUMEROUS ORGANISMS ON EARTH. 3. Organisms are classified as Bacteria by one characteristic: the lack of a cell nucleus (the name "prokaryote" means "before a nucleus") 4. Outer cell wall made of petidoglycan 5. Some move by means of a flagella (sing. flagellum) 6. Fimbrae - fibers that stick to surfaces (tooth decay, gonorrhea) 7. Region called the NUCLEOID which has a single circular chromosome, accessory rings of DNA called PLASMIDS REPRODUCTION Occurs by BINARY FISSION (mitosis) and CONJUGATION (exchange of DNA) TRANSFORMATION- bacteria incorporate genes from dead bacteria TRANSDUCTION - viruses insert new genes into bacterial cells. This method is used in biotechnology to create bacteria that produce valuable products such as insulin ENDOSPORES - during unfavorable conditions, bacteria enclosed in a protective coat (Ex. Tetanus NUTRITION & NEEDS Obligate anaerobes - cannot grow in the presence of oxygen Facultative anaerobes - can grow with or without oxygen Aerobic - require oxygen Photoautotrophs - photosynthetic Chemoautotrophs - obtain energy from oxidizing inorganic compounds, such as ammonia Chemoheterotrophs - decomposers Shape of Bacteria/ Naming Cocci - sphere Bacilli - rods Spirilla - spirals Staph - in clusters Strep - in chains Ex. Staphylococcus Gram Stain Gram's Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans Christian Joachim Gram, a Danish doctor. Gram's stain differentiates between two major cell wall types. Bacterial species with walls containing small amounts of peptidoglycan are GramnegativeBacteria with walls containing relatively large amounts of peptidoglycan are Grampositive. Gram Negative -- light red or pink color Gram Positive -- dark purple Escherichia coli, Salmonella typhi, Vibrio cholerae and Bordetella pertussis Staphylococcus epidermidis, Streptococcus pyogenes, and Clostridium tetani Not all bacteria can be stained by Gram's method, the best-known exception belong to the genus Mycobacterium which have waxy cell walls. How Gram Stains are Made: For more information on Gram Stains, see http://www-micro.msb.le.ac.uk/video/Gram.html Bacteria and Health - Some diseases caused by bacteria: tetanus | botulism | Black Plague | Tuberculosis |gonorrhea | syphilis| Lyme disease | Strep throat | Pneumonia | Anthrax |necrotizing fasciitis (flesh eating bacteria) | toxic shock syndrome The Usual Suspects *these are the names of specific bacteria you need to know for the test, and the diseases they cause Streptococcus lactis strep throat, related bacteria causes necrotizing fasciitis Staphylococcus aureas found on skin, responsible for minor infections (like on cuts/scratches) Bacillus subtilis common lab bacteria, easy to grown, unharmful Bacillus tetani causes tetanus (lockjaw) Bacillus botulism causes botulism (food poisoning) Bacillus pestis causes Black Plague Bacillus anthracis anthrax Mycoplasmas very very tiny, cause of pneumonia Rickettsia rickettsi link between bacteria and viruses, can't reproduce outside host, causes Rocky Mountain Spotted Fever Escherichia coli E. Coli - common bacteria of the digestive tract, also causes food poisoning Antibiotics and Antiseptics Joseph Lister created the first antiseptic, an acid to spray on tables and instruments before surgery (1860) The Discovery of Penicillin Alexander Fleming Noticed mold growing on petri dishes Bacteria did not grow where the mold was He isolated the chemical that killed bacteria, but it was not stable Howard Flory continued the work, later stabilized the chemical Fleming and Flory received the Nobel Prize in 1945 http://www.slic2.wsu.edu:82/hurlbert/micro101/images/101PhageLife.gif http://www.whfreeman.com/kuby/content/anm/kb03an01.htm Protists Protists belong to the Kingdom Protista, which include mostly unicellular organisms that do not fit into the other kingdoms. Characteristics of Protists: mostly unicellular, some are multicellular (algae) can be heterotrophic or autotrophic most live in water (though some live in moist soil or even the human body) ALL are eukaryotic (have a nucleus) A protist is any organism that is not a plant, animal or fungus Protista = the very first Classification of Protists how they obtain nutrition how they move Animallike Protists - also called protozoa (means "first animal") - heterotrophs Plantlike Protists - also called algae - autotrophs Funguslike Protists - heterotrophs, decomposers, external digestion Animallike Protists: Protozoans . Four Phyla of Animallike Protists Classified by how they move Zooflagellates - flagella Sarcodines - extensions of cytoplasm (pseudopodia) Ciliates - cilia Sporozoans - do not move Zooflagellates move using one or two flagella absorb food across membrane Leishmania Sarcodines Ameba (See Ameba Coloring Sheet) moves using pseudopodia ( "false feet" ), which are like extensions of the cytoplasm --ameboid movement ingests food by surrounding and engulfing food (endocytosis), creating a food vacuole reproducing by binary fission (mitosis) contractile vacuole - removes excess water can cause amebic dysentery in humans - diarrhea and stomach upset from drinking contaminated water Other sarcodines: Foraminferans, Heliozoans See Ameba Move Ciliates Paramecium: move using cilia has two nuclei: macronucleus, micronucleus food is gathered through the :mouth pore, moved into a gullet, forms a food vacuole anal pore is used for removing waste contractile vacuole removes excess water exhibits avoidance behavior reproduces asexually (binary fission) or sexually (conjugation) outer membrane -pellicle- is rigid and paramecia are always the same shape, like a shoe See a Paramecium Swim Sporozoans do not move on their own parasitic Malaria is a sporozoan, infects the liver and blood Parasitic Protists Parasite - an organism that lives on or in a host organism and causes harm to that organism Vector - an organism that can carry a parasite, and is responsible for infecting other organisms (host) with that parasite. Vectors themselves are not harmful, but in the battle against human disease, controlling the vector can control the transmission of parasites. Malaria Protist: Plasmodium Vector: Anopholes Mosquito Statistics: According to the World Health Organization, 300-500 million cases of malaria occur each year Malaria results in 1.5-2.7 million deaths per year (much more than AIDS) Most cases occur in Africa and South America Symptoms include fever, headache, vomitting and other flu-like symptoms The protist lives inside the bloodstrea, eventually clogging capillaries and destroying blood cells, which will lead to death if not treated The arrow points to the purplish colored protist (Plasmodium), the pinkish spheres are blood cells Anopheles moquisto taking a blood meal, this is how a human becomes infected with plasmodium and contracts Malaria African Sleeping Sickness (or Trypanosomiasis) Protist: Trypanosoma Vector: Tse Tse Fly Statistics: Occurs mostly in sub-saharan africa Symptoms include fever, headaches, pain in joints -followed by a phase when the parasite infects the central nervous system, causing confusion, lack of coordination, and uncontrolled sleepiness. Without treatment, the host will die For more information, see http://www.who.int/emc/diseases/tryp/trypanodis.html This slide shows a blood smear of a person infected with trypanosoma. The protist is the purplish colored string-like things. They appear string-like due to a flagella. The reddish circles are blood cells. Giardiasis Protist: Giardia Transmission: Drinking contaminated water (usually outdoor streams and other untreated water) Symptoms: Severe diarrhea and vomitting, the protist takes up residence in the digestive tract. B = Protist, Giardia A = flagella Other Protist Parasites Cryptsporidium - this protist was responsible for a major health crisis in Detroit when the city's drinking water became contaminated Amebic Dysentery - also known as Montezuma's Revenge, travellers often contract this in other countries (causes diarrhea) Questions for Thought 1. Does the United States have a responsibility toward treating and containing parasitic infections found in other parts of the world? 2. Why is controlling the vector important to control the disease? 3. One of the best ways to prevent many parasitic infections is to have a source of clean water. Why do you think many third world countries have more incidence of parasitic infection that other countries? Plantlike Protists: Unicellular Algae contain chlorophyll and carry out photosynthesis commonly called algae four phyla: euglenophytes, chrysophytes, diatoms, dinoflagellates accessory pigments help absorb light, give algae a variety of colors Euglenophytes Euglena live in water have 2 flagella for movement use chlorplasts for photosynthesis, but can turn into heterotrophs if they are kept in the dark has an eyespot used for sensing light and dark pellicle - like a cell wall, helps maintain their shapes Chrysophytes Diatoms Dinoflagellates yellow-green algae, "golden plants" produce thin cell Often have two flagella walls of silicon, luminescent main component of glass Ecology of Unicellular Algae make up the base of aquatic food chains phytoplankton makes up half of the photosynthesis that occurs on earth (oxygen) can cause Red Tides - algal blooms - which are toxic Plantlike Protists: Red, Brown, Green Algae Green Algae: Phylum Chlorophyta Unicellular green algae, Colonial (volvox), Multicellular (ulva, sea lettuce) Spirogyra live in water, multicellular named after a spiral shaped chloroplast autotrophic Funguslike Protists heterotrophs, decomposers called slime molds and water molds water molds responsible for the Irish Great Potato Famine http://biologycorner.com/bio1/protist_quiz.html http://biologycorner.com/concepts/kingdom_protista2.jpg Classifying Plants Nonvascular: have no vessels, no roots, no stems or leaves. Examples: Mosses & Liverworts Vascular: have vessels to transport food and water. They have roots, stems and leaves. Example: Grass, corn, trees, flowers, bushes Xylem: transports water Phloem: transports food & nutrients Gymnosperms "naked seeds" cone bearing plants (seeds grow on cones) needle like leaves usually stay green year round wind pollinated Examples: pine trees & evergreens Angiosperms Monocots Angiosperms have have 1 seed leaf (cotyledon) parallel veins on leaves 3 part symmetry for flowers fibrous roots Example: lilies, onions, corn, grasses, wheat flowering plants seeds are enclosed in a fruit most are pollinated by birds & bees have finite growing seasons Examples: grasses, tulips, oaks, dandelions Divided into two main groups: Monocots & Dicots Dicots Angiosperms that have 2 seed leaves (cotyledons) net veins on leaves flowers have 4-5 parts taproots Examples: trees and ornamental flowers Parts of the Plant Roots water and minerals are absorbed (taproots vs fibrous roots) also used to anchor the plant movement of water up to leaves is influenced by TRANSPIRATION Stems Support plant transport water through xylem transport nutrients through phloem a celery stalk soaked in food coloring will absorb the food coloring, you can see the xylem Two types of stems: herbacious and woody Leaves Photosynthetic organ of the plant, used to convert sunlight into food Photosynthesis Equation: Stomata: pores within the leaf that open to let CO2 in and O2 out. Guard cells open and close. Cuticle: waxy covering on leaf that prevents water loss Flower Reproductive organ of the plant Flowers are usually both male and female The male part of the flower is the STAMEN The female part of the flower is the PISTIL See your coloring sheet for more detail on flower anatomy Plant Reproduction Pollen is produced by the stamen. Pollen moves away from the plant via the wind or other pollinators (birds & bees) The pollen lands on the pistil of another plant and fertilizes the eggs within the ovary The flower petals fall off, the ovary develops into a FRUIT that encloses the seeds Fruits are dispersed in a variety of ways (wind, animals) Fruits are not always edible, anything with a seed inside can be considered a fruit (helicopters, acorns, dandelions) Asexual Reproduction in Plants Many plants can clone themselves, a process called VEGETATIVE PROPAGATION strawberry plants and other vine like plants send out runners, which grow into new plants some plant clippings will grow into new plants a Potato will grow into a new plant How Plants Grow Germination occurs when a seed sprouts (usually caused by changes of temperature and moisture) Monocots have 1 seed leaf (cotyledon), Dicots have 2 seed leaves Perennials - live serval years, and reproduce many times, woody plants are perennials Annuals - a plant that completes its life cycle in one growing season (grows, flowers, reproduces and then dies) Biennials - takes two growing seasons to complete, it reproduces in the second growing season Plants grow only at their tips in regions called MERISTEMS PRIMARY GROWTH makes a plant taller at roots and stems SECONDARY GROWTH makes a plant wider, or adds woody tissue Tree Rings tell the age of a tree, each ring represents a growing season. The photo shows a tree who has been through four growing seasons. The lighter thinner rings are winter periods. VASCULAR CAMBIUM: area of the tree that makes more xylem and phloem and forms the annual rings Anatomy of a Leaf - see coloring worksheet Functions Cuticle: waxy covering, prevents water loss Xylem: vascular tissue, transports water Phloem: vascular tissue, transports nutrients (phood) Stomata (stoma): pores used for gas exchange Guard cells: open and close stomata Mesophyll: middle tissue, cells have chloroplasts used for photosynthesis, mesophyll consists of the spongy and palisade layers Epidermis: layer of cells just under the cuticle Vein: a structure composed of xylem and phloem, veins run from the tips of the roots to the edges of leaves Anatomy of a Stem Monocots and Dicots differ in the way their vascular tissue is arranged Monocot Stem Dicot Stem A: vascular bundle (scattered thru stem) B: ground tissue: storage, support A: epidermis B: vascular bundle (arranged in a ring) C. ground tissue (pith) D. cortex http://biologycorner.com/worksheets/botany_wordsearch.html Chapter 26 - 1 Introduction to the Animal Kingdom Most diverse kingdom in appearance Each phylum has its own typical body plan (arrangement) What is an Animal? Animals are heterotrophic, eukaryotic, and multicellular and lack cell walls. 95% = invertebrates (do not have backbone) 5% = vertebrates (have a backbone) What Animals do to Survive Physiology = Study of the functions of organs Anatomy = the structure of the organism/organs Homeostasis is maintained by internal feedback mechanisms Feedback inhibition = the product or results of a process stops or limits the process Ex: dog panting releases heat There are 7 essential functions of animals: Feeding: Herbivore = eats plants Carnivore = eats animals Omnivore = eats plants and animals Detritivore = feed on decaying organic material Filter Feeders = aquatic animals that strain food from water Parasite = lives in or on another organism (symbiotic relationship) Respiration: Take in O2 and give off CO2 Lungs, gills, through skin, simple diffusion Circulation: Very small animals rely on diffusion Larger animals have circulatory system Excretion: Primary waste product is ammonia Liquid waste Response: Receptor cells = sound, light, external stimuli Nerve cells => nervous system Movement: Most animals are motile (can move) Muscles usually work with a skeleton Reproduction: Most reproduce sexually = genetic diversity Many invertebrates can also reproduce asexually = to increase their numbers rapidly Trends in Animal Evolution Cell Specialization and Levels of Organization: Cells -->tissues -->organs -> organ systems Early Development: Zygote = fertilized egg Blastula = a hollow ball of cells Blastopore = the blastula folds in creating this opening Protostome = mouth is formed from blastopore Deuterosome = anus if formed from blastopore Anus = opening for solid waste removal from digestive tract The cells of most animal embryos differentiate into three layers called germ layers Endoderm = (innermost) develops into the lining of the digestive tract and respiratory tract Mesoderm = (middle) muscle, circulatory, reproductive, and excretory systems Ectoderm = (outermost) sense organs, nerves, outer layer of skin Body Symmetry: Body Symmetry - the body plan of an animal, how its parts are arranged Asymmetry - no pattern (corals, sponges) Radial Symmetry - shaped like a wheel (starfish, hydra, jellyfish) Bilateral Symmetry - has a right and left side (humans, insects, cats, etc) Cephalization - an anterior concentration of sense organs (to have a head) *The more complex the animals becomes the more pronounced their cephalization anterior - toward the head posterior - toward the tail dorsal - back side ventral - belly side Segmentation - "advanced" animals have body segments, and specialization of tissue (even humans are segmented, look at the ribs and spine) Body Cavity Formation: A fluid-filled space where internal organs can be suspended Types of Animals Phylum Examples Evolutionary Milestone Porifera sponges multicellularity Cnidaria jellyfish, hydra, coral tissues Platyhelminthes flatworms bilateral symmetry Nematoda roundworms pseudocoelom Mollusca clams, squids, snails coelom Annalida earthworms, leeches segmentation Arthropoda insects, spiders, crustaceans jointed appendages Echinodermata starfish deuterostomes Chordata vertebrates notochord Sponges Kingdom Animalia Phylum Porifera Characteristics of Sponges · Simplest animals, multicellular · No organs or body systems · Cellular digestion · Asymmetry · Filter Feeders· Sessile (do not move) · Reproduce sexually (sperm and eggs) · Reproduce asexually (regeneration) · Skeleton composed of spongin (soft) and spicules (hard) **Sponge Anatomy (see handout) Amebocytes - cells within the sponge that move around supplying nutrients and taking away waste Choanocytes (collar cells) · layer of cells with flagella · the movement of the flagella keeps a water current going in the sponge · food vacuoles in the collar cells digest plankton and other small organisms (filter feeder) Oscula - large opening at top of sponge, water exits Ostia - small openings at the side, water enters Cnidarians Kingdom Animalia Phylum Cnidaria Examples: Jellyfish, hydra, sea anemone, coral, portuguese man of war Characteristics of Cnidarians · Tentacles · Cnidocytes (stinging cells) · Nematocysts (barbs) · Gastrovascular cavity (digestion) · Most are radial symmetry, some have asymmetry (corals) Cnidarians have two body forms: Polyp - stationary, vaseshaped Medusa - swimming, cup-shaped Examples: jellyfish, portuguese man of war Examples: hydra, coral, sea anemone Notes 27 (27-1, 27-2) FLATWORMS (27-1) Kingdom Animalia, Phylum Platyhelminthes (Flatworms) Three embryonic germ layers Bilateral symmetry Cephalization (head) Coelom (Greek for cavity or hollow) = a fluid filled body cavity Acoelomates = without coelom Form and Function in Flatworms Feeding: Free-living = carnivores or scavengers Have digestive cavity, mouth, pharynx Parasites: = Feed on blood, tissue fluids, or pieces of cells from within a host Most do not have a complete digestive system because they absorb digested material directly Respiration, Circulation, and Excretion: Thin bodies allow for materials to diffuse (resp, excrection..etc) Flame cell = specialized cells that remove excess water Response: Ganglia = group of nerve cells control the nervous system (like a brain) Eyespot = group of cells that can detect light Movement: Flatworms moves in 2 ways 1) Cilia = helps them glide through water and on stream floors 2) Muscle cells = twist and turn Reproduction: Sexual Reproduction = Hermaphrodites = has both male and female reproductive organs Asexual Reproduction by fission = organism spits in two GROUPS OF FLATWORMS 1. Class Turbellarians = free-living flatworms Fresh or marine water Example: Planarians (cross-eyed) Planarian (also known as Dugesia)--lives in freshwater --mostly a scavenger, also feeds on protists --hermaphrodites --they can regenerate (regrow parts), Reproduction by FISSION Anatomy of the Planarian Brain (ganglia) - planarian can process information about their environement Pharynx - used for suckling food in (the mouth is at the end of the pharynx) Eyespot - simple eye, can detect light Flame cells - located along the lateral edges, used for excretion Intestine - digestion (does not have an anus) 2. Class Trematoda = parasitic flatworms a.k.a "flukes" live in mouth, skin, or gills of host Primary host = the host in which a parasite reproduces sexually Intermediate host = the host in which asexual reproduction occurs Schistosoma mansoni - multiple host: Primary host = human Intermediate host = snail Causes Schistosomiasis -in humans; decays lungs liver, spleen, or intestines. Tropical areas with poor sanitation/sewage. 3. Class Cestoda =tapeworms Long, flat, parasitic Live in intestines Scolex = a structure that contains suckers and/or hooks Proglottids = body segments of the tapeworm Each mature proglottid is a hermaphrodite Testes produce sperm, fertilize the eggs to produce a zygote Zygotes are passed out through the feces. A dormant, protective cyst is formed in the intermediate cyst. This is why you should never eat incompletely cooked meat. 27-2 Roundworms Kingdom Animalia - Phylum Nematoda Unsegmented worms Pseudocoelom ("false coelom") - body cavity contains organs Digestive tract with 2 openings, mouth & anus Feeding Free-living - predators Parasites - humans and animals Reproduction: Sexual reproduction, Separate sexes (male & female) Roundworms & Disease Trichinosis (trichinella worm) - cysts within the muscles are consumed (undercooked food) -- worm grows in intestine -- forms cysts in the muscles of the new host -- symptom: terrible pain in muscles Filarial Worms - found in Tropical regions of Asia -- usually transmitted by mosquitoes -- causes elephantiasis Ascarid Worms (common roundworm) - lives in intestine - eggs are passed out in the feces Hookworms - burrow into the skin from soil - mature in the intestines --hooks used to attach and suck blood Research on C. elegans - first organism to have DNA completely sequenced Sec. 27-3 Annelids Phylum Annelida segmented worms, true coelom -Includes: earthworm, marine worms, leeches Annelids -Annelida means "little rings" -body divided into segments separated by septum Septum=internal wall - some have bristles called setae on each segment -have closed circulatory system -have well-developed nervous system with brain & nerve cords -sexual reproduction, some are hermaphrodites *See Earthworm Anatomy Dissection Sheets Groups of Annelids 1. Class Oligochaeta - Earthworm -Streamlined bodies (p. 697 fig. 27-16) -Few setae -Live in soil or fresh water -As earthworms pass food & soil through intestines, nutrients are absorbed and indigestible matter passes out through anus as castings = earthworm feces -Castings - enrich soil, earthworms aerate soil 2. Class Hirudinea - Leeches -external parasites with suckers on each end - suck blood & body fluids from host -most live in moist tropical habitats -medicinal uses (circulation, anti-clotting) 3. Class Polychaeta - Marine worms See p.698 fig.27-18 -includes:sandworms, bloodworms, & relatives -have paired paddlelike appendages with setae Notes: 27-4 Mollusks Both have a true coelom (body cavity) Similar larval stage - trochophore Bilateral Symmetry Organ systems Characteristics of Mollusks: · Visceral Mass (organs) · Mantle (outer body layer) · Foot (muscle, movement) · Shell in most · Radula - tongue-like structure, sharp · Gills for respiration · Most have separate sexes Types of Mollusks: · Gastropods - snails, slugs, nudibranchs ("stomach foot") · Bivalves - clams, oysters ("two doors") · Cephalopods - nautilus, cuttlefish, squid, octopus ("head foot") Arthropods Characteristics Makes up 3/4's of all animal species -total number of arthropod species is MORE than all other species combined Includes insects, spiders, & crustaceans Arthropod means "jointed foot" - all arthropods have jointed appendages Segmented body Exoskeleton for protection & support Exoskeleton is shed during molting Open circulatory system Compound eyes (has many individual units) Excretory structures called Malpighian tubules Wings in many groups Respiration using spiracles and trachae Segmentation More obvious in larval forms, adults have fused segments -----> Head | Thorax | Abdomen Some have a fused head and thorax -- the cephalothorax Taxonomy Subphylum Chelicerata Includes (Class Arachnida) spiders, ticks, scorpions, mites and horseshoe crabs Have a cephalothorax (fused head& thorax) and abdomen No antenna Spinnerets in spiders make webs Have 6 pairs of jointed appendages: * Chelicerae - claws or fangs (1 pair) - (spiders have venom) * Pedipalps - used for feeding, sensing, transferring sperm (1 pair) * Walking legs movement (4 pairs) Subphylum Crustacea Marine members include shrimp, lobster, barnacles, & crabs Terrestrial crustaceans called isopods (pillbugs or rollypollys) Freshwater members include crayfish and daphnia All have mandibles for chewing or tearing Have cephalothorax & abdomen Lobsters and large custraceans are called Decapods Barnacles are sessile (don't move) Have 10 pairs of jointed appendages Breathe through gills Crayfish Dissection covers more on the Crayfish Anatomy Subphylum Uniramia - Insects and their Relatives Includes 3 classes --- Chilopoda (centipedes), Diplopoda (millipedes), & Insecta Class Chilopoda Centipedes (predators) Name means "100 legs" Flattened body Have 1 pair of legs per body segment Pincers can inject venom Class Diplopoda Millipedes Name means "1000 legs" Have 2 pairs of legs per body segment Rounded body Scavengers or herbivores Class Insecta 3 pairs of legs 3 body parts - head, thorax, abdomen Wings in most All appendages attach to the thorax 9-11 segments in abdomen Mandibles for chewing Insect Life Cycle Metamorphosis - dramatic physical change Complete metamorphosis: Larva --> pupa ---> adult (example: butterflies) Incomplete metamorphosis: Egg --> nymph --> adult (example: crickets) Social Insects: Honeybees (workers, queen, drones). Termites, some wasps Queen: Lays eggs (1 queen per hive) Drones: a few males to fertilize eggs Workers: all infertile females Chapter 30-1 .............The Chordates Key characteristics: -Dorsal, hollow nerve cord -Notochord (usually present only in embryo) -Pharyngeal pouches -paired structures in throat; may develop into gills -Tail - extends beyond anus About 96% of all chordate species belong in one subphylum: Subphylum Vertebrata- Vertebrates ---Animals with a backbone or vertebral column (endoskeleton) ----Have spinal cord - dorsal, hollow nerve cord ----Front end of spinal cord develops a brain Nonvertebrate Chordates -- 2 subphyla of chordates without backbones: Subphylum Urochordata -Tunicates (see fig. 30-3 & 4 pg.769) -Filter-feeders in ocean -Only larval tunicates have chordate characteristics Subphylum Cephalochorodata -Lancelets (see fig.30-5pg. 770) -Small fishlike animals -Adult lancelets have chordate characteristics -Have definite head region Lancelet Sea Squirts Timeline of Vertebrate Evolution About When Age Animals 550 million years ago Ordovician Period First vertebrates jawless fishes 400 million years ago Devonian Period Acanthodians jawed fishes "Age of Fishes" 350 million years ago Carboniferous Period (and Permian) Amphibians "Age of Amphibians" 240 million years ago 60 million years ago 340,000 years ago Triassic Period Jurassic Period reptiles appeared "Age of Dinosaurs" dinosaurs dominated the land for 150 million years - sauropods, theropods, etc.. Tertiary Period Dinosaurs extinct "Age of Mammals" Mammals appeard Quaternary period Humans appeard ________________________________________ FISH (ch30-2) CHARACTERISTICS - Aquatic - Paired Fins - Gills, Scales EVOLUTION OF FISHES - 1st Fish were jawless - Devonian Period - "Age of Fishes" - Jaws & Paired fins improved swimming and feeding - Cartilage Skeletons - Bony Skeletons (Modern Fish) FORM & FUNCTION Feeding - Heterotrophs (carnivores, herbivores, omnivores, detritivores, parasitic) Respiration - Gills and Gill Covering (operculum) - Lungfishes (air-breathers) Circulation - Closed Circulatory System, Single Loop - Atrium --Ventricle -- Gills -- Body -- Back to Atrium Excretion - Salt water fish tend to lose water - Fresh water fish tend to gain water - Homeostasis maintained by the kidneys Response - Cerebrum - thinking, voluntary activities - Cerebellum - coordination - Medulla Oblongata - functions of internal organs - Lateral Line System - senses vibrations Movement - Paired Fins - Swim Bladder - buoyancy FINS Caudal Dorsal Anal Pelvic Pectoral Reproduction - Oviparous (lays eggs) - Ovoviviparous (eggs stay in mom) - Viviparous (babies get nourishment from mom. Ex. Humans, cats, some fish) Groups of Fish Kingdom Animalia Phylum Chordata Subphylum Vertebrata 2 Classes of Jawless fish: - Lamprey (parasitic) - Hagfish (scavenger) - Both have cartilage skeleton Class Chondrichthyes - Cartilage Fish - Sharks, stingrays *Bio 111 – see Lab Notes - Most are predators - Basking sharks are filter feeders - No swim bladder, pectoral fins rigid Class Osteichthyes - Bony Fish *Bio 112 – See Perch Notes - Ray-finned ( Goldfish, Bass, Carp, Salmon )& Lobe Finned ( Coelacanth ) Chapter 30 - Amphibians *Herpetology is the study of reptiles and amphibians What is an amphibian? - 4000+ species - Gave rise to modern land vertebrates - Amphibian means -double life- Larvae start life in H2O with gills , adults are terrestrial with lungs Evolutionary adaptations for life on land: 1. stronger bones 2. lungs and breathing tubes 3. sternum (breastbone) and ribs to protect internal organs History: Carboniferous Period = Age of Amphibians, 360-290 million years ago Climate changes caused habitats to disappear 3 orders of amphibians survive today; 1. Frogs and Toads 2. Salamanders 3. Caecilians Form and Function in Amphibians Feeding: larvae = herbivore, adults = mostly carnivore Digestive tract; mouth > esophagus > stomach > small intestines > large intestine (colon) > cloaca Respiration: larva = skin and gills, adult = lungs and some through skin Many terrestrial salamanders = no lungs at all, through skin and mouth cavity Circulation: double loop system ( See figure 30-24 ) 3 chamber heart right atrium, left atrium, and ventricle Compare Single to Double Loop Circulation Single Heart --> Gills --> Body Double Heart --> Lungs --> Heart --> Body Excretion: kidneys filter liquid waste = urine Kidneys > ureters > small urinary bladder > cloaca Reproduction: females lay eggs in water, male deposits sperm over eggs Tadpoles Frogs Herbivorous Aquatic Single Loop Gills Carnivorous Terrestrial or Aquatic Double Loop Lungs Yolk of egg nourishes developing embryo Larvae commonly called tadpoles A few species will care for their eggs by incubating their young in their mouth, on their back, or stomach! Metamorphosis see figure 30-25 Response: well developed nervous and sensory system ( See figure 30-26) 1. Eyes move in socket and have a protective structure = nictitating membrane is a transparent membrane that covers the eye when the frog is in the water 2. Tympanic membrane = eardrums 3. Lateral Line systems = detect water movement (vibrations) Groups of amphibians (see figure 30-27) Kingdom Animalia ....Phylum Chordata .........Subphylum Vertebrata ..............Class Amphibia Order Urodela (Salamanders and Newts) long bodies and tails, lives in moist woods Mud puppy keeps gills and lives in water all their lives Order Anura (Frogs and Toads) hop/jump with legs, adult has no tail Order Apoda (Caecilians) legless with fishlike scales Ecology The number of living species is declining; environmental threats such as decreasing habitats, fungal infections, introduced predators, increasing human population Frog Stuff Frogs are carnivorous and feed on insects and worms. Larger frogs even prey on birds and small mammals. Some frogs eat other frogs. See a frog eating a mouse. Frogs always swallow prey whole - they do not chew. Poison Dart frogs have bright coloration to warn predators that they are poisonous. [ Strawberry Poison Dart Frog ] Frogs vs. Toads - Frogs tend to have slimy skin and live in water. Toads have dry bumpy skin. See the frog gallery to compare the two. If you're interested in different species of frogs check out this animal site: Frogs & Toads Frogs are called bioindicator species - since water and oxygen goes across their skin, pollution in the environment causes deformities and other problems. Scientists measure the health of an environment by looking at the health of the frogs. See deformed frog. Another deformed frog. Surinam frogs have a spongey layer of skin on their back, tadpoles develop within The Darwin Frog carries its young in its mouth Chapter 35 - Reptiles Kingdom Animalia ---Phylum Chordata ------Subphylum Vertebrata ---------Class Reptilia Characteristics of Reptiles 1. Strong, bony skeletons and feet with claws 2. Ectothermic (cold-blooded) 3. Dry scaley skin 4. Amniote eggs 5. Respiration with lungs 6. Ventricle partially divided 7. Internal fertilization The Amniote Egg Contains a water and food supply for the developing embryo and can be layed on land. Must be fertilized internally, has a shell Structure Function amnion provides a watery environment for the embryo yolk Sac contains the food for the embryo allantois stores waste chorion allows oxygen to enter and carbon dioxide to leave albumin egg white Oviparous - eggs are laid and incubated outside the body Ovoviviparous - eggs are incubated inside the body, born live Viviparous - live birth, no egg (humans) Types of Reptiles Types of Lizards (click to see a picture, then use the back button to return) Komodo dragon - largest lizard (monitors), also contains toxic saliva and is thought to be the closest relative to snakes - it has a forked tongue Gecko - group of lizards that often have suction cups on feet. Frilled lizard - has a large frill of skin around the neck Chameleon - has the ability to change color (anoles can also do this) Gila Monster - a venomous lizard, bites can cause illness, even death - found in Mexico and some southern states Beaded lizard - venomous lizard Horned lizards - shoot blood from their eyes as a defense mechanism (also called horned toads) Snakes Evidence suggests that snakes evolved from lizards that burrowed. Snakes retain small legbones even though they have no legs. Snakes have the ability to unhinge their jaw and swallow prey much larger than them. See a snake swallow an egg. Constrictors: not venomous, prey is killed by squeezing and suffocating; Examples: pythons, boa constrictors, king snakes Some constrictors can get extremely large, like the anaconda Other common constrictors you might even find in your backyard: Black snake - these snakes can get very large and live in fields and open spaces, they eat rats Garter snake - very common in this area and have a characteristic yellow stripe. These snakes are not venomous, but you should never try to pick one up, they will bite. Venomous Snakes Not very many snakes are venomous, they fall into four groups 1. Cobras and coral snakes - inject a neurotoxin that causes paralysis and breathing difficulties Check out the Interactive King Cobra at NationalGeographic.com 2. Sea snakes -even though they spend most of their life underwater, these snakes breathe air, their tails are shaped like a paddle to help them swim 3. Adders and vipers 4. Rattlesnakes and water moccassins (also called cottonmouths) -inject a hemotoxin which causes destruction of the blood and tissue, the area of the bite becomes swollen and dark. Copperheads also fall into this group - and are common to the region you live in, you are not likely to enounter any other type of venomous snake in granite city. Snakebites: Neurotoxins (cobra, coral snake) | Hemotoxins (vipers) The Anatomy of a Snake Snakes are adapted to be long and skinny. All of their organs are elongated and compact. The spine can be made of several hundred vertebrae Like amphibians and birds, snakes have a cloaca Jacobson's Organ - located at the roof of the mouth, this organ helps the snake process odors. A flick of a forked tongue sends chemical signals to the Jacobson's organ - snakes often track prey by smell Pit organ - located on the head of some snakes, it detects heat and is used to track prey in the dark Turtles and Tortoises Characterized by a shell that is fused to the turtle's spine - it cannot be removed, nor can a turtle crawl out of it. The top of the shell is the carapace, the bottom of the shell is the plastron. Turtles - generally live in water, have flat streamlined bodies. Sea turtles migrate long distances to lay their eggs at the same beach they were born at. Tortoises - live on land and have rounded bodies Crocodiles and Alligators Crocodilians are most closely related to dinosaurs They are the only reptile group that takes care of their young. Adapted to stealth hunting - eyes and nostrils are above the head, so the body can remain submerged, they attack when animals (or humans) come to the water shore to drink Crocodiles have a pointier snout and alligators have a snout that is more rounded. Crocodile teeth also stick out where as alligator teeth fit into a socker. See a pic to compare them Types of Crocodilians Alligators Crocodiles Caiman Gavial - note the elongated snout The Tuatara (order Rhynchocephalia) known as a "living fossil" - they have survived unchanged for 150 million years has a third eye to detect heat only lives in New Zealand and is in danger of becoming extinct Tuataras have distinctive head spikes - See Pic Mammals Classification Characteristics Kingdom Animalia ----Phylum Chordata ------Subphylum Vertebrata ---------Class Mammalia About 16 Orders in three groups Egg-Laying Mammals, Pouched Mammals, Placental mammals Hair Endothermy 4 chambered heart Diaphragm (muscle to aid breathing) Most nourished by a placenta Mammary glands produce milk Gestation - length of time within the uterus (9 months for humans) Weaning - time at which young stop drinking milk Marsupials - Order Marsupiala Most groups live in Australia - mainly because Australia split from the main continent and became isolated, the species there are almost all marsupials, though humans have "imported" other groups now Opossum - the only marsupial that exists naturally outside of Australia Some Australian Marsupials Kangaroo Phalanger Koala Wombat Convergent Evolution - marsupials and placentals resemble each other because they evolved in similar habitats, thus having adaptations for similar lifestyles. The Circulatory System (Chapter 30) The Vertebrate Heart Unlike the heart of a fish, the human heart is a DOUBLE LOOP. That is, it goes from the heart, to the lungs, back to the heart, and then to the body. (In fish, blood goes from the heart, passes through the gills and onto the rest of the body) Double loop circulation is more efficient, because it gives the heart and extra pump to move blood to the body The mammalian heart has 4 chambers, so that oxygenated and deoxygenated blood is completely separated - again increasing efficience. The hearts of amphibians and reptiles have only 3 chambers, blood mixes in the ventricle. (Though crocodiles do have a 4 chambered heart) The heart is roughly the size of your fist in humans The Anatomy of the Human Heart The heart is divided into 4 chambers: 1. Right Atrium (RA) 2. Right Ventricle (RV) 3. Left Atrium (LA) 4. Left Ventricle (LV) (largest part of the muscle, pumps blood to rest of body) The right side of the heart collects oxygen poor blood and pumps it to the lungs The left side of the heart collects oxygen rich blood (from the lungs) to the rest of the body Valves Tricuspid valve- between right atrium and right ventricle Bicuspid valve - between the left atrium and the left ventricle Semilunar (Pulmonary valve) - is at the exit of the Right Ventricle to pulmonary artery Semilunar (Aortic valve) - is at the exit of the Left Ventricle to the Aorta Contractions Systole - ventricle contracts, pumping blood to the lungs and to the body Diastole - atrium contracts, pumping blood to the ventricle Flow of Blood (see figure 30.5) Blood from the body flows: 1. to the Superior and Inferior Vena Cava, 2. then to the Right Atrium 3. through the Tricuspid Valve 4. to the Right Ventricle 5. through the Pulmonic Valve 6. to the Pulmonary Artery 7. to the Lungs The blood picks up oxygen in the lungs, and then flows from the lungs: 1. to the Pulmonary Veins 2. to the Left Atrium 3. through Mitral valve 4. to the Left Ventricle 5. through the Aortic Valve 6. to the Aorta 7. to the body Major Vessels Aorta - pumps blood to the body (it connects to the left ventricle) Pulmonary artery - connects to right ventricle, pumps oxygen-poor blood to the lungs Pulmonary veins - bring oxygen rich blood from the lungs to the left atrium Superior Vena Cava - brings oxygen poor blood from the upper body to the right atrium Inferior Vena Cava - brings oxygen poor blood from the lower part of the body to the right atrium Coronary Arteries - located on the outside of the heart, these vessels supply blood to the heart itself, a blockage in these arteries can lead to a heart attack. Other Resources If you want some good graphics and explanations of the heart, check out http://www.howstuffworks.com/heart.htm - HIGHLY RECOMMENDED!Heart Animations http://medlib.med.utah.edu/kw/pharm/hyper_heart1.html http://www.medtropolis.com/VBody.asp http://www.thequalityhospital.com/cgiwin/mercyweb.exe/heart_animation.htm Try the Practice Quiz on the Heart Images Veins and Arteries Veins and Arteries2 Heart and Vessels - double loop Heart (unlabeled) What about Blood? Blood is a mixture of cells and plasma. Its job is to supply the body with oxygen and nutrients and to carry away waste. Blood cells Red Blood Cells carry oxygen from the lungs White blood cells help fight infection Platelets are used for clotting Plasma The liquid portion of the blood, contains nutrients and vitamins, hormones, and proteins. *The human body contains approximately 5 liters of blood Mini-Review Questions: 1. Trace the blood flow in the human heart. 2. Name the vessels that leave the heart 3. Why is the human heart called a "double loop" 4. What are the two components of blood? 5. Name the valves of the heart and describe their location. 6. Compare systole to diastole. http://school.discoveryeducation.com/quizzes6/muskopf/humanheart.html