- 129 -
Biology (Mwit’14)
Research Title
Researchers
Advisors
Department
School
The Effectiveness of Terminalia catappa Leaves on Bubble Nest Production
of Siamese Fighting Fish
Miss Kittiya Paungpitikul,Miss Thanutra Zhang, Miss Thummaporn Boonvisudhi
Miss Sataporn Wantanawijarn
Biology
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The study examined the effectiveness of Terminalia catappa leaves on the production of
bubble nest of Siamese fighting fish. Three kinds of water were set; 1) water dipped with Terminalia
catappa leaves 2) water boiled with Terminalia catappa leaves and 3) clean pipe water. The Siamese
fighting fish were fed in each water kind. The results showed that those fed in water dipped with
Terminalia catappa leaves produced the largest area of bubble nests which were 61.22 square
centimeters; whereas those fed in water boiled with Terminalia catappa leaves and clean pipe water
produced 31.51 and 12.45 square centimeters of bubble nests, respectively. The fish in water dipped
and boiled with Terminalia catappa leaves produced longer bubble nests than those in clean pipe
water. Furthermore, the bubble nests in water dipped and boiled with Terminalia catappa leaves
stayed longer than those in clean pipe water. The sizes of bubble nests in three water kinds were not
different.
- 130 -
Biology (Mwit’14)
Research Title A Study of Foods Effect on Planarian Populations Dugesia sp.
Researchers
Miss Patrawee Tangprasert, Miss Sirikorn Pamonsupornvichit and
Miss Wichurat Sakulpaptong
Advisors
Miss Woranis Klintong, Mr. Bancha Sabaitua, Miss Messuwan Pongpramoon, Mr.
Sakchai Kamarangkoon, and Miss Tulaporn Buranasomphop
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
This study identifies types of food which result in the proliferation of planarians Dugesia sp.
Planarians were separated into 4 groups; red-worm-fed, water-flea-fed, chicken-liver-fed, and boiledegg-yolk-fed. Planarians were fed 2 times a week over a period of 4 weeks, and then they were
counted. After conducting the experiment 15 times for accuracy, the results showed that the amount
of water-flea-fed planarians increased more than red-worm-fed, chicken-liver-fed, and egg-yolk fed
planarians, respectively. However, the body size-structure of chicken-liver-fed planarians increased
more than red-worm-fed, egg-yolk-fed, and water-flea-fed planarians, respectively. The pH of
planarian water used with planarians was measured and found that the water-flea-fed water had an
average pH of 8.6, while the boiled-egg yolk feeding water had an average pH of only 7.9. The pH
result indicated that the optimum pH of water for feeding planarians is around 8.3-8.6. In conclusion,
sorts of food which produce this pH range are best for fostering the growth of planarians.
- 131 -
Biology (Mwit’14)
Research Title The Relationship between the Hemispheric Dominance and 9 Human abilities of
M.4 and M.5 Mahidol Wittayanusorn School Academic Year 2006 Students
Researchers
Mr. Phakhawet Techatewon and Mr. Marut Laohaviroj
Advisors
Miss Montakan Subkaew, Miss Tanyaratana Dumkua, Miss Tawashini Rojanavi
and Assistant Professor Ph.D. Usanee Anurutwong
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The comparison of the exploration of the hemispheric dominance by using the test created by
the researchers and by Assistant Professor Dr. Usanee created had the same result at 54.33%. When
comparing the tests by allowed 2 points for errors, the tests has the same result of 64.72%. When
using the researchers test and the 9 human abilities test, There are more percentage of students who
are left hemispheric dominant and have Mathematical abilities than right hemispheric dominant
students. But there are right hemispheric dominant students who have Bodily ability, Visual ability,
Linguistic ability and Emotional ability more than left hemispheric dominant students. When
analyzed by using the Pearson’s correlation, almost all of the human relate to the others, especially in
both hemispheric dominant students. When analyzed by using the multiple regression using all
students and divided students into groups according to their sex and educational level. The result
shows that sex is related to the hemispheric dominance because the male students has multiple Rsquared more than all students 2.3%, the female students has multiple R-squared more than all
students 5.5%, the M.5 students has multiple R-squared more than all students 0.8% and the M.4
students has multiple R-squared more than all students 1.5%. In conclusion, the Mathematical ability
influences students to be left hemispheric dominant; moreover, the Mechanical ability, Technical
ability and Auditory ability influence students to be right hemispheric dominant and the others
depend on valuables that use for dividing. Thus, the relationship between the hemispheric dominance
and 9 human abilities also depend on other factors and each of 9 human abilities relate to having one
the other abilities.
- 132 -
Biology (Mwit’14)
Research Title Effect of Temperatures and Ultraviolet Radiation on Hatchability Rate of Yellow Fever
Mosquito’s eggs (Aedes aegypti)
Researchers
MissDudrudee Chaiittiporn, Mr.Parin Jirapatrasilp and Mr.Pakorn Aiewsakul
Advisors
MissSataporn Wantanawijarn, MissTanyaratana Dumkua and Mr.Winyoo
Phanmuangma
Department
Biology
School
Mahidol Wittayanusorn Academic Year 2006
Abstract
Environment is one of the factors affecting the survivorship of the living things, including
mosquitoes’ hatchability. Therefore, the changes of physical factors would affect the hatchability. In
the studying of effect of temperatures on hatchability rate of Aedes aegypti’s eggs, the eggs were
heated for 15 and 30 minutes, with the different temperatures: 35, 40, 45, 50 and 55oC. There were no
statistical difference of the hatchability rate between eggs heated for 15 and 30 minutes. By further
experiment of comparing the results of different temperatures on the hatchability rate, the eggs heated
for 30 minutes with 35, 40, 45, and 50 oC had the rate more than 0 percent, but the relation between
temperatures and hatchability rates could not be concluded definitely. The eggs heated with the
temperature of 55 oC had the rate of 0 percent. In addition, by the studying of effect of Ultraviolet
radiation on hatchability rate of the mosquitoes’ eggs, UVA affected the rate more than UVC. The
hatchability rates of the eggs exposed to Ultraviolet for 15, 30 and 60 minutes trended to decrease but
stayed above 0 percent.
- 133 -
Biology (Mwit’14)
Research Title
Researchers
Advisors
Department
School
The Astaxanthin extract affect the survival rate of Tub Tim fish
Mrs. Supakan Jiranapakul Mr. Chaipat Treeratsakulchai and
Mr. Pongpat Metapipattanakul
Ms. Orawan Piyaboon Ms. Sattaporn Wantanawijarn and
Ms. Tanyaratana Dumkua
Biology
Mahidol Wittayanusorn
Academic Year 2006
Abstract
Nowadays the technology of feeding Tub Tim fish is not good enough. This causes the high
death rate of Tub Tim fish. Recent years ago, it was found that astaxanthin, found in Haematococcus
pluvialis algae, affects the survival rate of the fish. This experiment was conducted by culturing
Haematococcus pluvialis algae and extracting astaxanthin by acetone, non-polar solution which can
dissolve non-polar substance named astaxanthin. Next, mix the extracted solution with fish food and
feed the fish of age 1-2 months old and 2-3 months old. When compare to the control group in which
the fish are fed with no astaxanthin, it can be found that astaxanthin affects 1-to-2-month-old Tub
Tim fish more extremely than on 2-to-3-month-old Tub Tim fish. This is resulted from the low
immunity that 1-to-2-month-old Tub Tim fish have. Hence, astaxanthin helps increase the fish’s
immunity by acting as antioxidant. However, 2-3 month-old Tub Tim fish are stronger than 1-2
month-old Tub Tim fish.
- 134 -
Biology (Mwit’14)
Research Title Studying the Efficiency of Weeds in Wetland Wastewater Treatment
Researchers
Miss Waraporn Anuparp u-krit, Miss Thanchanok Ratvijitvect and
Miss Paleerat Wattana u-dom
Advisors
Mr. Bancha Sabuytua, Miss Tanyaratana Dumkua, and Miss somruthai Homchuen
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The purposes of this study were to investigate the efficiency in wastewater treatment of three
kinds of weeds: Eichhornia crassipes, Solms [Water Hyacinth], Cynodon plectostachyus [Star grass]
and Ruellia tuberosa L. [Solanum spiral] in constructed wetlands for 4 days. The efficiency was
evaluated by comparing color, odor, pH, DO and BOD of influent wastewater with effluent. Unit with
Water Hyacinth was the best performance for wastewater treatment which could be the most
decreasing pH from 7.71 to 7.50, increasing DO to 1.21 mg/l and decreasing BOD to 51.5 mg/l
- 135 -
Biology (Mwit’14)
Research Title
Researchers
Advisors
Department
School
The study on effect of crude extract from Andrographis paniculata to
the growth of Erwinia carotovora subspecies carotovora
Mr. Witchanon Dontisanor Mr. Suttipong Thongsuwan and
Mr. Wuttapon Sadaeng
Miss Orawan Piyaboon Miss Tanyaratana Dumkua and
Miss Somruthai Homcheun
Biology
Mahidol Wittayanusorn
Academic Year 2006
Abstract
One of important herbal plant, which is used for inhibiting microbial growth, is
Andrographis paniculata. Nowadays, crude extract from A. paniculata was used in many researches
for inhibiting microbial. Two types of A. paniculata were used for extracting in order to inhibiting
Erwinia carotovora subspecies carotovora growth. Fresh aerial part and dried aerial part were
extracted by three types of solvents; distilled water, ethanol 95% and acetone; correspondingly, 6
crude extracts were prepared. They were tested with E. carotovora at concentration 0.265% 2.5% 5%
20% 25% 50% 75% (w/v) by Bauer – Kirby Method (paper disk diffusion). Testing result showed
that all crude extracts at various concentrations could not inhibit the growth of E. carotovora,
however there are many researches supported that the crude extract from A. paniculata could inhibit
Escherichia coli which is in the same family as E. carotovora. In order that, the ability of receptors of
each bacteria were different from each other or the crude extract from A. paniculata from this
extraction decomposed before using.
- 136 -
Biology (Mwit’14)
Research Title The Study of Circinate Movement and Relation Between Length, Weight and
Number of Segments of Thyropygus allevatus
Researchers
Mr. Soravit Changpinyo, Mr. Chumsaeng Chumsaengsri and Miss. Nattaporn
Setasatian
Advisors
Miss Orawan Piyaboon, Miss Tanyaratana Dumkoh,
Miss Sataporn Wantanawijarn, and Miss Amporn Boonyasatitsataporn
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The study of circinate movement and relation between length, weight and number of
segments of Thyropygus allevatus was found that the best stimulated position is the head segment, the
stimulated position and the time they used curling are not significantly relative. Their behavior,
stimulating with pendulum, is considered learning behavior called habituation. In the first stage,
millipedes responded to the stimulus longer. Then, they did less, no circinate movement when
stimulated.
It was also founded that the average length, weight, and number of segments were 126.7608
mm, 13.8447 g, and 51.8333, respectively. Their big size and large number of segments could be
considered a dead phase of growth. Moreover, length and number of segments were normally
distributed [k-s test]. Millipede’s weight correlated to its length and number of segments with r =
0.8060 at p < 0.01. The weight prediction equation is W = 12.084 – 0.391N + 0.173L (W is weight
[g]; N is number of segments; L is length [mm])
- 137 -
Biology (Mwit’14)
Research Title Ability of Turmeric Extract for Inhibiting Growth of Erwinia carotovara supsp.
carotovora, the Causal Agent of Soft Rot
Researchers
Ms. Pilaipan
Advisors
Ms.Orawan Piyaboon, Ms. Sataporn Wantanawijarn and Ms. Tanarat Dumkoh
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
Efficacy test of turmeric extract for inhibiting growth of Erwinia carotovora supsp.
carotovora, the causal agent of soft rot was conducted. In the experiment process, Erwinia
carotovora supsp. carotovora were culture on double layer nutrient agar (double layer NA), then, the
extracted substances that were extract by using 95% ethyl alcohol and varied in five level, 750 mg/ml,
500 mg/ml, 250 mg/ml, 100 mg/ml and 50 mg/ml would be brought in testing method aiming to
evaluate the rate of bacterial inhibition efficiency. As the experimented, turmeric extract, extracted
by using 95% ethyl alcohol, was tested on growth inhibition of Erwinia carotovora supsp.
carotovora, could not show inhibition zone on bacteria culturing in double layer NA.
- 138 -
Biology (Mwit’14)
Research Title Comparative Study in Anatomy of Parkia speciosa and Parkia timoriana
Researcher Ms.Intuorn anupantanan and Ms.Kanokwan Limmangkun
Advisers
Ms.Somruthai Homcheun , Ms.Metsuwan Pongpramun , and
Mr.Win-yu Panmoungma
Department Biology
School
Mahidol Wittayanusorn
Academic
2006
Abstract
Comparative study in anatomy of Parkia speciosa and Parkia timoriana focused on root,
stem and leaf. Paraffin method is used to make slides of the plants’tissues. The researchers found that
in primary root of Parkia speciosa , there are 7 arches of xylem. In contrast , Parkia timoriana have 5
arches of xylem. In secondary root , while Parkia speciosa has sclerenchyma , there is no
sclerenchyma in Parkia timoriana. In primary stem , Parkia speciosa has 1 layer of epidermis. In
contrast , Parkia timoriana has 2 layer of epidermis. In secondary stem , Parkia timoriana has the
smaller circle of pith with phloem sclerenchyma next to periderm. In leaf , both are bifacial which
consist of palisade parenchyma and spongy parenchyma. In the apical meristem , both share the same
type and tissue arrangement.
- 139 -
Biology (Mwit’14)
Research Title Comparative Anatomy of Root, Stem, Leave and Petiole of Variegatum
(Piper sarmentosum Roxb.) and Long Pepper (Piper chaba Hunt.)
Researchers
Mr. Kitti Wuthisathid, Mr. Jira Jindalertudomdee and
Mr. Panchnok Srinualnat
Advisors
Miss. Somruthai Homchin, Mrs. Sataporn Wantanawijarn and
Mr. Sarawut Sangurai
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
In a study of the comparative anatomy of root, stem, leave and petiole of Piper sarmentosum
Roxb. and Piper chaba Hunt. by using Paraffin Technique. It was found that there are many different
characteristics. For example, in P. sarmentosum the trichome exists in the vegetative organ of all
samples and the blade has a subepidermic layer in abaxial and adaxial face epidermis, . In P. chaba
no trichome exists, the blade presents a uniseriate epidermis and a subepidermic layer in the abaxial
face. The P. sarmentosum chlorenchyma is next to epidermis in the stem and that of P.chaba is
separated from the epidermis. The P. sarmentosum sclerenchyma is near by phloem and that of
P.chaba is near by xylem. On the other hand, two species show some structural likenesses in that both
have polyarch in root, one or two concentric rings of collateral bundle in stem and one open bundle in
leaf and petiole.
- 140 -
Biology (Mwit’14)
Research Title A Search for the γ -Tocopherol Methyltransferase Production Gene in Five Thai
Fruit Plants
Researcher Mr. Manuswin Chansakulporn
Advisors
Miss Tanyaratana Dumkua, Miss Somrutai Homchuen, Miss Woraya Kwaiphan,
Mr. Sakchai Kammarangkool and Dr. Supatcharee Netrphan
Department Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
α -Tocopherol, a structure of Vitamin E, is an antioxidant in human body thus it helps many
systems to work properly. In α -Tocopherol biosynthesis, the last enzyme that is necessary for the
plant to synthesize is γ -Tocopherol methyltransferase (γ -TMT). In Thailand, the deficiency of
Vitamin E is found in elder people, especially in the northeastern region where there is a very high
level of the deficiency. The study about the ability of synthesizing Vitamin E in Thai fruit plants is a
way to reduce that problem. The α -Tocopherol synthesis was determined by using molecular
procedure to search for γ -TMT production gene in five Thai fruit plants that were Guava, Mango,
Rambutan, Spanish plum and Tamarind. From the experiment, Guava was the only one Thai fruit
plant that did not have this gene. After having searched for Nucleotide sequence of γ -Tocopherol
gene in other plants, it had the fractions of intron squeeze through this gene. The average of percent
similarity of other Thai fruit plants was 78.1 percent except Mango that has only 37.4 percent, when
compared with soybean’s γ -TMT cDNA sequence. Rambutan one of two sequences had similarly
high percentage to Tamarind that is 99.9 percent when compared with each other. From the
Phylogenetic tree, it was possible that one γ -TMT gene of Rambutan and γ -TMT gene of Tamarind
had very close development. However, this research will have a complete conclusion in case I have
some data of the nutrients in these Thai fruit plants, but such a study in Thai fruit plants is very few so
I can only conclude that Mango, Rambutan, Spanish plum and Tamarind have an ability to synthesize
α -Tocopherol that is very useful for human body.
- 141 -
Biology (Mwit’14)
Research Title Biocontrol of Cutworms (Spodoptera litura) Using Entomopathogenic Fungi
Beauveria bassiana
Researchers Mr. Chin Suppapinyaroj, Mr. Paramet Kongpitak and Mr. Sorakrit Atcharanuwat
Advisors
Miss Orawan Piyaboon Miss Thanyaratana Dumku Miss Woranitt Klinthong and Dr.
Tipwadee Attathom
Department Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The cutworm, Spodoptera litura, is one of the pests that cannot be easily controlled. To
eliminate caterpillars by using insecticide, more pollute will be accumulated in the environment. In
the research, it shows that Beauveria bassiana is one of the fungi which are very specific to insects
and harmless to humanity – the most advantage characteristic to people. In the experiment, it was
divided into replications, which were sprayed with fungi’s spores at various concentrations
(1x106 1x107 and 1x108 spores/ml). The result would be recorded within 7 days and analyzed. It was
founded that the efficiency of the fungi suspension at 1x106 1x107 and 1x108 spores/ml could kill the
caterpillars at 47.76%, 71.1% and 70% respectively. Furthermore, It showed that fungi suspension
could kill the caterpillars more than 50% in the 1x107 spores/ml and 1x108 spores/ml experiment
(P = 0.019 and P = 0.035). Conclusively, the least concentration of Beauveria bassiana suspension
that could kill the caterpillars efficiently was 1x107 spores/ml.
- 142 -
Biology (Mwit’14)
Research Title Bio-Fertilizer Tablet from Golden Apple Snail
Researchers
Miss Noppawan Tunsirimas, Miss Prapatsara Prakarnkamanant and
Miss Pichita Prasongvej
Advisors
Miss Sataporn Wantanawijarn, Mr. Bancha Sabuytua, and
Miss Woranit Klintong
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The Golden Apple Snail (Pomacea canaliculata) is one of the important plant enemies in
Thailand and they are spreading out to the farmland throughout the country. Thus, many experiments
are being conducted to eradicate the Golden Apple Snail and make uses of their remains by
processing them as liquid bio-fertilizer. After that the fertilizer can be reprocessed into fertilizer
tablets for convenience and easy storage by pounding 3 kilograms of Golden Apple Snail into a
thick paste, then mixing it with molasses at a ratio of 1:1 leaving to ferment for 120 days. The
fertilizer is mixed with three separate bonding agents: flour, soil and ground bran of rice each in ratios
of 1:1, 1:2 and 2:1. Testing for the ability to bond without access moisture, the proportion of 1:1 was
found to be best. We then tested for the ability to dissolve and molder well and found the tablets with
flour to be best. For fertilizing efficiency, the three types of the fertilizer tablets were tested with the
Kale vegetables (Brassica oleracea var. alboglabra Bailey) producing similar results.
- 143 -
Biology (Mwit’14)
Research Tittle A Medium Agar from Water Chestnut
Researchers
Mr. Pathomchat Sirirug, Mr. Natthaphat Rojanasupamit and
Mr. Padoongpong Chomthong
Advisors
Miss Sataporn Wantanawijarn Miss Orawan Piyaboon
and Miss Woranit Klintong
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
Nowadays, the quantity of water chestnut (Eleocharis dulcis Trin.), one of Thailand’s
agriculture products, is more than requirement therefore their price is getting low. There are some
information tell that water chestnuts compound of a lot of carbohydrate. For increasing water
chesnuts’ value, researchers try to use them as material to produce medium agar with the same
method as producing PDA (Potato Dextrose Agar) . Lentinus strigosus, Genoderma lucidum,
Pleurotus sajor-caju and Pleurotus hungarian were investigated for cultured in PDA. It was found
that the fastest grown up muchroom is Lentinus strigosus. Concentration of the medium agar from
water chestnut was investigate for culturing Lentinus strigosus. It was found that the best
concentration of the medium agar from water chestnut is 0.25 kg/dm3. PDA and 0.25 kg/dm3 water
chestnut dextrose agar were compared for culturing four mushrooms; it was conclude that the result
of mushrooms grown on PDA and 0.25 kg/dm3 water chestnut was in the invicity.
- 144 -
Biology (Mwit’14)
Research Title A Survey Study of Drinking Water Quality in Mahidol Wittayanusorn School
Researchers
Mr. Chanwit Aphibanborirak Mr. Tanachat Nilanon and Mr. Parit
Kanjanavirojkul
Advisors
Miss Thanyarat Dumkoh Mr. Sorachai Saelim and Mr. Wallop Kongna
Department
Biology
School
Mahidol Wittayanusorn
Academic Year
2006
Abstract
A survey study of drinking water quality in Mahidol Wittayanusorn School had taken quality
evaluation at 7 drinking sites which are canteen, 7th floor of building 1, 5th floor of girl dorm.* 1, 3rd
floor of girl dorm. 2, B-section 6th floor of boy dorm., G-section 5th floor of boy dorm., and first aid
center. Data were collected four times in July, August, November, and December of 2006. The
sampled water was tested in temperature, acidity, hardness, total bacteria, and MPN values. The
measured temperatures varied between zero and 15 °C. Measured acidity had the minimum value of
6.92 and the maximum value of 7.88. For hardness analysis, averaged value of some sites was out of
standard. Specifically at 3rd floor of girl dorm. 2 and 7th floor of building 1, the evaluated total
hardness was higher than the standard total hardness for bottled water. Hardness of collected water
from canteen gradually increased month by month. In standard plate count analysis, no datum was
perceived to be out of standard. Some sites were found to have MPN index values out of standard in
August and December. Especially, G-section 5th floor of boy dorm., in August, has MPN index value
far more than that of drinking water standard.
- 145 -
Biology (Mwit’14)
Research Title
Researchers
Advisors
Department
School
The Study of Music Types Effect on Growth in Ipomoea aquatica Forsk
var. reptans
Miss Kanjana Sukhokanjanachusak, Mr. Artchapong Hassametto and
Miss Somkamol Dolbandalchok
Miss Somruthai Homcheun, Miss Tunyaratana Dumkua and
Mr. Bancha Sabaytua
Biology
Mahidol Wittayanusorn
Academic Year
2006
Abstract
Three music types; classic, rock and Chinnabunchorn supplication, affect the growth of
Ipomoea aquatica Forsk var. reptans. Using the classic music can give its highest average growth of
18.05 cm height. The height average of Chinnabunchorn supplication and rock music are 17.40 and
17.12 cm, respectively. Three methods of sound wave given to I.aquatica are direct method, passingthrough-water method and combination method. The height average of passing-through-water
method, combination method and direct method are 18.05, 17.61 and 16.90 cm, respectively. After
the experiment, it is found that calculated result by One-way ANOVA is not significant (p>0.01).
- 146 -
Biology (Mwit’14)
Research Title
Researchers
Advisors
Department
School
The Study of Music Types Effect on Growth in Ipomoea aquatica Forsk
var. reptans
Miss Kanjana Sukhokanjanachusak, Mr. Artchapong Hassametto and
Miss Somkamol Dolbandalchok
Miss Somruthai Homcheun, Miss Tunyaratana Dumkua and
Mr. Bancha Sabaytua
Biology
Mahidol Wittayanusorn
Academic Year
2006
Abstract
Three music types; classic, rock and Chinnabunchorn supplication, affect the growth of
Ipomoea aquatica Forsk var. reptans. Using the classic music can give its highest average growth of
18.05 cm height. The height average of Chinnabunchorn supplication and rock music are 17.40 and
17.12 cm, respectively. Three methods of sound wave given to I.aquatica are direct method, passingthrough-water method and combination method. The height average of passing-through-water
method, combination method and direct method are 18.05, 17.61 and 16.90 cm, respectively. After
the experiment, it is found that calculated result by One-way ANOVA is not significant (p>0.01).
- 147 -
Biology (Mwit’14)
Research Title A Study of the Portable Homemade Leaf Dryer’s Effectiveness
Researchers
Mr.Kasama Rakpechmanee and Mr.Pongrit Sothikulnun
Advisors
Mr.Winyu Punmuangma, Ms.Messuwan Pongpramool and
Ms.Somrutai Homchuen
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
Traditional method of making herbarium specimens—an essential process for studying
plants of botanists, has been relatively difficult, required expensive tools such as a hot air oven for
drying and taken much time. Importantly, collected specimens can be easily damaged by certain
causes related to humidity, temperature, etc. There are two purposes of the study: to invent a portable
homemade leaf dryer with lower cost, less weight and using a few days to dry specimens and to study
the effectiveness of the invention. The portable homemade leaf dryer has composed of plywood,
aluminum plates and electronic equipments—heaters and thermostats as the main components. The
effectiveness of the portable homemade leaf dryer was measured in terms of the specimens’ relative
humidity after being dried by the leaf dryer compared with using a Hot Air Oven. Brake Fern
( Pteris ensifomis cv. Victoriae) leaves were selected as the plant specimens of the experiment and
dried for 12 hours. The portable homemade leaf dryer weighs about 2.8 kilograms, costs about 2,000
baht a unit, is easy to carry and able to use 12 volt car battery as energy source and takes about 3
hours after being switched on in order to have slightly fluctuated temperature around 60oC. The study
shows that the specimens’ relative humidity after being dried by the leaf dryer was 48%, whereas the
relative humidity of those dried by Hot Air Oven was 54%. This report brings together all known
records of making herbarium specimens using the portable homemade leaf dryer that it is an
interesting new way out for botanists which can well be used instead.
- 148 -
Biology (Mwit’14)
Research Title Effect of Chitosan on the Growth of Cos by Hydroponic
Researchers
Miss Patrinee Thamsriswadi, Miss Wanwara Thuptimdang and
Miss Sunisa Sae-ko
Advisors
Miss Somruthai Homcheun, Miss Orawan Piyaboon, and
Mr. Sarawut Saengurai
Department
Biology
School
Mahidol Wittayanusorn
Academic Year
2006
Abstract
In studying the effect of chitosan on the growth of cos (Lactuca sativa L. var. longifolia) by
hydroponics; there are 4 groups of samples containing both the nutrient A and B and chitosan varied
in concentrations of 0, 0.01, 0.1 and 1 ppm respectively. The growth of cos was measured once a
week. After the fifth week, it was found that the cos grew effectively in chitosan concentrate 0.01,
0.1, 1 and 0 ppm respectively.
- 149 -
Biology (Mwit’14)
Research Title
Researchers
Advisors
Department
School
The Comparision of Ability to Prohibit Ralstonia solanacearum from Different Types
of Allium Extraction
Ms. Kitsara Niamhom, Ms. Sirom Piyasirinon
Ms. Tanyaratana Dumkua, Ms. Orawan Piyaboon and Ms. Woranit Klintong
Biology
Mahidol Wittayanusorn
Academic Year 2006
Abstract
Ralstonia solanacearum is a kind of bacteria causing Bacteria Wilt in economic plants. Hence,
it is imperative to control bacterial growth. Nowadays, chemical substances are used to control these
bacteria. Therefore, founding natural extract would make lots of benefits. Beginning Study was found
that Allium can restrain bacteria. Thus, this project was conducted to compare ability of Allium extract
consisting of Shallot, Onion and Multiply Onion to restrain R. Solanacearum. We used paper disc
diffusion to test the efficiency of these three Allium extract in halting R. Solanacearum . The result
was not like what we expected because the clear zone did not appear. Then we used the serial dilution
method. We could see that R. Solanacearum was restrained by three Allium extracts, Shallot, Onion
and Multiply Onion, at the least concentration of 12.5, 12.5 and 6.25 percent by mass in order.
- 150 -
Biology (Mwit’14)
Research Title The Study of the Last Concentration of Honey Solution that Can Inhibit the
Growth and Kill Yeast Candida albicans which Founded in Human’s Mouth and
Throat
Researchers
Miss Prapaipan Putthapiban, and Mr. Panupong Chaijitsakul
Advisors
Miss Orawan Piyaboon, Miss Sataporn Wantanawijarn, and MissThanyaratana
Dumku and Assistant Professor Pitak Santanirand
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The effectiveness of honey in many of its medical uses is due to its antibacterial activity such
as high osmotic pressure, acidic environment, and other chemical agents. Moreover, honey is the
most popular bee products therefore using honey in order to inhibit and kill microorganism in
human’s mouth and throat especially Candida albicans, the most common microorganism in human’s
mouth and throat. The only problem that occurs is when the number gets out of control and cause
Candidiasis for example Oral thrush and Angular chelitis. In the experiment to find out the least
concentration of honey solution that can inhibit the growth and kill Candida albicans of Longan
honey that harvested in 2005, Longan honey that harvested in 2006 and Bitter bush honey that
harvested in 2005, the honey solutions were diluted with Sabouraud’s dextrose broth (SDB) in 0% to
100% v/v. The turbidity of honey solution was observed by comparing with undiluted solution. The
least concentration that is clear, was consider to be the HIT (Honey Inhibitory Traitor) Furthermore,
all clear solutions were streaked on Sabouraud’s dextrose agar (SDA). The least concentration that
prevented the growth of Candida albicans colony-forming was considered to be the HFT (Honey
fungicidal traitor). The HIT of 3 types of honey range from approximately 40% to 60% while HFT
range from approximately 60% to 100% .They are no significantly different among 3 types of honey
at 95% confidence interval.
- 151 -
Biology (Mwit’14)
Research Title To Inhibit the Laying Eggs of Papilio demoleus by the Extract of Neem
Researchers
Mr. Chaiyawat Supasilp and Mr. Prachya Piyasirisin
Advisors
Miss Orawan Piyaboon, Miss Tanyarat Damkao and
Miss Sataporn Wantanawijarn
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
In the test of neem’s efficiency to inhibit the laying eggs of Papilio demoleus, there are 2
cages that have the kumquats (one tree for one cage). One cage gets the neem’s extract and one cage
doesn’t. Then, bring a couple of butterfly (male and female) to each cage for let them have a
reproduction and lay eggs. According to the experiment in three times of three generations of
butterfly, the amount of eggs and larvae (that hatch from eggs) on the kumquat that has been sprayed
the extract are less than the amount on the kumquat that hasn’t been sprayed one. Thus, the extract of
neem is able to inhibit the laying eggs of papilio demoleus.
- 152 -
Biology (Mwit’14)
Research Title Influence of Food Supply on Longevity and Fecundity of Trichogramma confusum
Researchers
Miss Sarin Visase
Advisors
Miss Sataporn Wantanawijarn, Miss. Tanyaratana Dumkua, and Mr. Winyoo
Phanmuangma
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
In studying the influence of food supply on longevity of female and male parasitoid wasps
(Trichogramma confusum) and fecundity of female parasitoid wasps which were observed by the
number of egg laid on the rice moth eggs (Corcyra cephalonica Stainton). Four types of food supply
at 8 weight percent concentration (water, sucrose, palm sugar and coconut sugar) were provided to the
parasitoids. It was found that the longevities of female wasps fed with sucrose and palm sugar were
longer than those fed with water and coconut sugar at significant .05 level. In addition, it was found
that the females’ fecundity when fed with sucrose was higher than that fed with water, palm sugar and
coconut sugar at significant .05 level. However, in the males’ treatment, it was found that every food
supply did not influence on longevity.
- 153 -
Biology (Mwit’14)
Research title Salt of Tartar in Gramineae
Researchers Miss Panita Maturavongsadit, Miss Nutcha Jintaganon and Miss Darin
Lanlakornsiripan
Advisors
Miss Tanyarat Dumkua, Miss Somretai Homcheun and Miss Sasinee Aunganon
Department Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The extent to how much the amount of Salt of Tartar, impure compound of potassium
carbonate, could be obtained in each plant and what are the factor of these differences, was
investigated by Gramineae plants. Four kinds of Gramineae plant were selected, Cogon grass
( Imperata cylindrica Beauv ), Couch grass (Cynodon dactylon Pers), Buffalo grass (Brachiaria
mutica (Forsk.) Stapf) and Corn (Zea mays (L.)) as control. These plants were burned in furnace. Salt
was extracted by dissolving the ash from furnace then evaporate the solutions. For potassium’s
amount solely, Atomic Absorption Spectroscope was used for measurement the amount of potassium
in each plant by wet digestion method. The quality of extracted salt was identified by finding fraction
between potassium carbonate, observed by reaction of the product of heating ash dissolved in HCl
and BaCO3, and overall amount of extracted salt. Factors of salt accumulation were investigated by
finding pH parameter, ECe parameter and total potassium amount of soil in each specimen.
In the results, salt extracted from every specimen shared the same characteristics, clear
crystal with some dark colored ash. Buffalo grass provided the highest amount of salt of tartar. The
rest were cogon grass, couch grass and corn respectively. From the fact that potassium is more likely
to bond with carbonate than other anion. It was reasonable to assume that salt of tartar would be
found in buffalo grass the most. The amount of salt extracted was affected by soil’s salinity.
- 154 -
Biology (Mwit’14)
Researchers Title Medium Agar from Scaphium scaphigerum
Researchers
Miss Nitima Petchana and Miss Supasuda Assawajaruwan
Advisors
Miss Sathaporn Wanthanawijan, Miss Orawan Piyaboon and
Miss Woranit Klintong
Department
Biology
School
Mahidol Wittaynusorn
Academic Year 2006
Abstract
After soaked in the water shape of sumlong ( Scaphium scaphigerum ) is similar to agar.
Therefore, using it to develop medium agar by using instead of agar. And then taking sumlong
flesh to dry out and crush. After that becomes dust. Another part, it is taken to liquidity by
spinnig. This project has four formulars using sumlong flesh and another is PDA. In our purpose
would like to compare the medium agar using sumlong flesh with PDA. By means of observing
the growth of fungi from diameter and density ’s fungi fiber within eleven days. The project uses
three kinds of fungi for testing such as Huguang, Hungary, and Nangfa. In conclusion found
that the forth formulars which consists of sumlong liquid 800 cm3 ( 145 g ) , potato 200 g and
glucose 20 g is the best.
- 155 -
Biology (Mwit’14)
Research Title Organogenesis of Aptenia Cordifolia in vitro
Researchers
Miss Acharawan Prakornsakul and Miss Sasichol Prukviwat
Advisors
Miss Somruthai Homcheun, Miss Aree Sak-yim
Miss Woranit Klintong, Assoc. Prof. Dr. Prasart Kermanee and
Assoc. Prof. Dr. Malee Nanakorn
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The organogenesis in a leaf of Baby Sun Rose (Aptenia cordifolia), during the first thirty
days of its life cultured in vitro using MS (Murashige and Skoog) (1962) medium was studied. The
specimens were collected every three days and fixed in a 50% solution of FAA (Formaldehyde Acetic
Acid). Paraffin method was used to maintain cross-section specimens. Results obtained showed that
the organogenesis occurred only in the petiole. The organ that developed was a root. The
organogenesis first occurred in the vascular cambium and the phloem of the petiole and was seen as a
small group of cells. Because of cell division, the group of meristematic cells was pushed away from
the vascular bundle. The new vessels formed at the apex of the root near the group of meristermatic
cells. The development of vessels occurred toward the old vascular bundle where they joined this
bundle forming the complete root.
- 156 -
Biology (Mwit’14)
Research Title The Effect of Urea Fertilizer Concentrations on the Efficiency of Trichoderma
harzianum in Eliminating Pythium aphanidermatum in Chinese Kales
Researchers
Miss Pim-orn Wacharaprapapong, Mr. Waradon Sungnak and
Mr. Numchok Sasigornwong
Advisors
Miss Orawan Piyaboon, Miss Tanyaratana Dumkua and
Miss Somruthi Homchuen
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
In studying the effect of urea fertilizer concentrations on the efficiency of Trichoderma
harzianum in eliminating Pythium aphanidermatum, a plant pathogen causing the damping-off
disease in a Chinese kale, and on the growth of the infected plant, various concentrations of the
fertilizer along with the two fungi were applied. After 30 days of cultivation, the soil specimens were
collected from each experimental group for dilution plate count and the plants were harvested for
biomass measurement. The result shows that the variation of the concentration within 0.3 g/l of water
from that the Thai farmers use, which is 0.75 g/l, did not significantly affect the number of
T. harzianum colonies, and that the difference within 0.15 g/l of water did not affect the average
Chinese kales’ biomass significantly at the significant level of 0.05. The aftermath also indicates that
although the increases in urea fertilizer concentrations raise the biomass of the Chinese kales, the
death rate caused by the infection also rises. On the contrary, the decreases in the other ones leads to
the reduction in the death rate but the survivals showed signs of malnutrition and were likely to die
later.
- 157 -
Biology (Mwit’14)
Research Title The Efficiency of Mangosteen’s Rinds to Inhibit the Growth of Bacteria
Xanthomonas campestris pv. Glycines
Researchers
Miss Ratchaporn Mengern, Miss Pakaporn Damrongkulchat and
Mr. Phanumas Krisorn
Advisors
Miss Orawan Piyaboon, Miss Tanyaratana Dumkoh, and
Miss Woranis Klinthong
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
In studying the efficiency test of plant crude extracts on growth inhibition of bacteria
Xanthomonas campestris pv. Glycines (Xc), the causal agent of bacterial pustule in soybean, Xc was
cultured in Nutrient Agar (NA) and then was tested with crude extracts from mangosteen peel at the
concentrations of 100,000 ppm 200,000 ppm 300,000 ppm 400,000 ppm and 500,000 ppm, each of
which was dissolve in 40% 60% and 80% ethyl alcohol. Diameter of each inhibited are were
measured to test the efficiency of the extracts by Paper Diffusion Method. The results suggest that the
concentrations of 100,000 ppm 200,000 ppm 300,000 ppm 400,000 ppm and 500,000 ppm were able
to inhibit the growth of Xc. Thereby, the crude extracts from mangosteen peel can inhibit bacteria
Xanthomonas campestris pv. Glycines.
- 158 -
Biology (Mwit’14)
Research Title Effect of Chitosan on Fat Oxidation in Pork
Researchers
Miss Nuttaporn Suteerapongpan, Miss Nichamon Yongwattana and
Mr.Rattanachai Ramaitithima
Advisors
Ms. Sataporn Wantanawijarn, Ms. Woranis Klintong, Mr. Sarawut Saengurai and
Dr. Pranee Lertsutthiwong
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
This study was focused on the feasibility study of the utilization of chitosan for the reduction
of fat oxidation in pork. Chitosan with different concentration (0.25%, 0.5% and 1%) was sprayed on
the shank of pork with the size of 4x6x1 cm3 and kept at 4C for 14 days. The determination of
oxidation in terms of Thiobarbituric Acid Value was done at the date of 0 3 5 7 and 14 respectively.
The results showed that chitosan could reduce the decay smell of pork, especially 1 % chitosan. It
could reduce the oxidation of fat in pork about 50% compared to the control (0.45 % acetic acid) after
storage at 4 C for 14 days
- 159 -
Biology (Mwit’14)
Research Title The Efficiency Assessment of Thai Herbal Volatile oils to Attract Oriental
Fruit Fly (Dacus dorsalis Hendel
Researchers
Miss Kullaya Takkawatakarn, Miss Natcha Wipaskatanyu
Advisors
Mr.Buncha Sabuytua, Mr. Winyu Panmaungma, and Miss Nithikarn Kiming
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The oriental fruit fly (Dacus dorsalis Hendel) is one of the serious pests that cause the
damage to agricultural products in Thailand. It exists all over the country and in every season. Thus,
there is a strategy using Methyl Eugenol (the synthetic sex pheromone of oriental fruit fly) to attract
the oriental fruit fly and capture by the trap. This chemical substance can be found in many kinds of
Thai herbs which are cheap, useful and easy to find. Therefore, we conducted the experiment which
was divided into three main parts. The first part was to study the suspension of the trap from the
recycle plastic bottle while the second part was to study the most appropriate period of time to attract
the oriental fruit fly. As for the last part, it was conducted to study the efficiency of 4 oriental fruit
fly’s attractants; Methyl Eugenol and 3 volatile oils from Thai herbs (white holy basil, red holy basil
and betel-vine).
According to the experiment, we found that the vertical suspension of the trap is better than
the horizontal suspension because the odor of attractants can be spread very well in all directions. The
best period of time to attract the oriental fruit fly is between 10.00-12.00 am. because of its
temperature. The olfactory bulb of oriental fruit fly works most efficiently at the temperature of 2628 ํ C. Furthermore, the Methyl Eugenol can best attract the oriental fly while three Thai herbal
volatile oils are much less effective. Due to the effect of eugenol, it can be concluded that the
attractant containing more eugenol can consequently attract a more number of oriental fruit flies.
- 160 -
Biology (Mwit’14)
Research Title The Study of Learning and Memorizing Behavior of the Persian Rabbit
(Oryctllagus cuniculas).
Researchers
Mr. Jittawat Pulputtapong , Miss Tosaporn Sattasathuchana and
Mr. Wittawat Fungtongjalern
Advisors
Miss Tunyaratana Dumkua, Mr. Win-yu Panmoungma, and
Miss Aree Sak-Yim
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
The rabbit has become one of the most popular mammals to keep as pets over the past
decade. Nevertheless, there have been a few behavioral experiments using rabbits as subjects. Thus,
studying the behavior of rabbits becomes especially interesting. This study investigated the learning
and memory of the Persian rabbit (Oryctllagus cuniculas). In this research, rabbits had to find the
correct arm in a changeable six-radial arm maze by opening 2, 3 and 4 arms, respectively. The
observed rate of success showed that Persian rabbit was unable to learn 2- and 3-radial arm mazes.
However, significant reduction in observed success was recorded when 4 arms were opened
representing that an apparent memory threshold had been crossed. In conclusion, the ability of the
Persian rabbit to learn some discrimination highlighted the existence of learning and Memorizing
Behavior of Persian rabbit.
- 161 -
Biology (Mwit’14)
Research Title Involvement between Contact Force and Responses of Sensitive Plant (Mimosa
pudica)
Researchers
Miss Rujapak Sutiwisesak
Advisors
Miss Somruthai Homcheun Mr. Suwat Sriyotee Miss Nitikarn Kim-ing
Mr. Somporn Buaprathoom and Mr. Pranotporn Arnilbon
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
In accordance with the study about involvement between contact force and responses of
sensitive plant (Mimosa pudica) by using equal mass of water dropped off onto the leaves of
Sensitive Plant from different heights and measuring the responses, the preliminary experiment was
conducted at three different heights, 1, 3 and 5 centimeter (cm). At each height, 30 examples were
studied. Following findings were recorded:
1. At 1 cm, average time used in complete closing was 3.64 seconds, average time used in
complete opening back was 8.48 minutes, and average number of closing leaflets is one pair.
2. At 3 cm, average time used in complete closing was 4.39 seconds, average time used in
complete opening back was 9.70 minutes, and average number of closing leaflets is 5.167 pairs.
3. At 5 cm, average time used in complete closing was 5.11 seconds, average time used in
complete opening back was 10.46 minutes, and all pairs of leaflets closed.
4. Other observations included (1) the most responsive area was the tip of each leaf and (2)
the average minimum height that could generate the response was 0.8 cm.
SPSS was used in the statistical analysis of the preliminary experiment findings. Following
results were recorded that at 1 and 3 centimeter and at 1 and 5 centimeter, the responses of the leaves
differed significantly, but at 3 and 5 cm, the responses of the leaves differed, yet not significantly.
Random factors which may have had effects on the experiment and its findings include that
air might have been a resistant force that caused water to lose energy while dropping down.
- 162 -
Biology (Mwit’14)
Research Title Biomagnifications of Hormone Fluoxymesterone in Food Chain to Sex Reversal
of Guppy (Poedilia reticulata)
Researchers
Mr. Puttigun Poungcharoen
Advisors
Miss Sataporn Wantanawijarn, Miss Tanyaratana Dumkua, and
Mr. Bancha sabuytua
Department
Biolgy
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
In studying of Fluoxymesterone, male sex hormone, accumulation in food chain for sex
reversal of guppy (Poedilia reticulata), by feeding chlorella sp. and rotifer in 10 milligrams of
Fluoxymesterone per 50 milliliters of water for 2 hours, after that fed water flea by added hormone
rotifer for feeding 3-day guppy once a day within 25 days and observed the sex of guppy, comparing
with guppy, fed by directly added hormone water flea, resulted that guppy, fed by directly added
hormone water flea showed secondary male characteristics, bright and color, before guppy which fed
by water flea that fed by rotifer that feeding with chlorella in hormone solution.
- 163 -
Biology (Mwit’14)
Research Title Characteristics and Relationships between Pollen and Stigma Structures of 7
Plants in Sirirukhachart Garden, Mahidol University
Researchers
Mr.Narunat Worasaktayanan, Mr.Thanate Assawakawintip and Mr.Therasate
Bunthuchai
Advisors
Miss Somruthai Homechuen Mrs. Sataporn Wantanawijarn
And Mr.Winyoo Panmuangma
Department
Biology
School
Mahidol Wittayanusorn
Academic Year 2006
Abstract
There are two vital reproductive structures of plants; a pollen grain and a stigma. The former,
found in anther, is a microspore inside which contains male gametophyte. The latter is the top part of
pistil that traps pollen grains in the pollination process. These two structures of plants play important
roles in the reproduction process of angiosperms. Realizing and interested in this fact, we decide to
study the correlation between stigma and pollen grain and analyze the structures of them by means of
accumulating samples of flowers, taking photos and conducting permanent pollen slides by using the
method of acetolysis. According to the result, the morphologies of both pollen grains and stigmas of
each plant vary significantly. However, those of the same species have very close relation; they have
appropriate structures that are conducive to adhering them together.
- 164 -
Biology (Mwit’14)
Research Title
Researchers
Advisers
Department
School
The Crude Extract from Alpinia conchigera Inhibits Staphylococcus
saprophyticus
Miss Niramol Tettiyu Miss Woranan Netkueakul and
Miss Sarisa Na pompetch
Miss Somruthai Homshuen Miss Orawan Piyaboon and
Miss Thanyarut Dumkoe
Biology
Mahidol Wittayanusorn
Academic Year
2006
Abstract
Staphylococcus saprophyticus is bacteria that is often found in symptomatic urinary tract
infection. Studying and using the crude extract from rhizomes of Alpinia conchigera inhibiting
Staphylococcus saprophyticus was the purpose. Rhizomes of Alpinia conchigera were extracted
by ethyl alcohol 95 percent. The efficiency of the crude extract was tested by using agar diffusion
method with five concentrations 0.1, 0.2, 0.3, 0.4 and 0.5 grams per milliliter and the control group
with only ethyl alcohol 95 percent. Compare the antibacterial efficiency between Staphylococcus
saprophyticus and Staphylococcus aureus. The result of antibacterial testing was that the crude
extract from rhizomes of Alpinia conchigera at 0.5 grams per milliliter is the most efficacious against
bacteria, Staphylococcus saprophyticus and Staphylococcus aureus.