Lab 5
Frog Cardiovascular Physiology: Computer Simulation - Exercise 34B
Human Cardiovascular Physiology: Blood Pressure and Pulse Determinations
- Exercise 33A
Frog Physiology
Activity 1: Recording Baseline Heart Activity, 1, 2
Activity 2: Investigating the Refractory Period of Cardiac Muscle, 4 only
Activity 3: Examining the Effect of Vagus Nerve Stimulation, 1, 2, 3, 4, 5
Activity 4: Assessing the Effect of Temperature 1, 2, 3, 4, 5, 6
Activity 5: Assessing the Effect of Pilocarpine 1, 2, 3, 4, 5, 6
Activity 6: Assessing the Effect of Atropine, 1, 2, 3, 4, 5
Activity 7: Assessing the Effect of Epinephrine, 1, 2, 3, 4, 5
Activity 8: Assessing the Effect of Digitalis, 1, 2, 3, 4, 5
Human Physiology
Activity 1: Auscultating Heart Sounds, 1, 2, 3
Activity 2: Palpating Superficial Pulse Points
Activity 5: Using a Sphygmomanometer to Measure Arterial Blood Pressure Indirectly,
1, 2, 3, 4, 5, 6
Name____________________________
Lab Section ________________
Activity and Review Sheet
Exercise 34B
1. Match the labels on the figure with the terms below:
___ Direct heart stimulator
___ Stimulator holder
___ Atrial contraction
___ Stimulus rate selector
___ Force transducer
___ Vagus nerve stimulator
___ Frog heart
___ Ventricular contraction
___ Oscilloscope
___ Voltage selector
____________________________________________________________
E
A
F
G
H
B
I
C
D
J
Follow the directions in the lab manual and answer the following questions.
2. What was the baseline heart rate in beats per minute? ________
3. a. When the stimulus was applied at a rate of 20 stimuli per second in Activity 2, what
was the result? (What did you observe happening?)
b. Draw an extrasystole and the compensatory pause that follows. Label the
extrasystole and the compensatory pause.
c. Using your drawing, when does the extrasystole occur? Circle one.
During contraction of cardiac muscle
During relaxation of cardiac muscle
d. What is the function of the compensatory pause?
e. Why is it important that the heart muscle cannot be stimulated to fused tetanus?
4. a. What effect did vagal stimulation have on heart rate?
___________________
b. What is the neurotransmitter for the vagus nerve? _________________________
5. Based on your data, what general statement can you make about the effect of
temperature on heart rate?
6. a. What was the effect of pilocarpine on heart rate? ___________________
b. What is the term for a drug that simulates the parasympathetic nervous system by
enhancing acetylcholine release? (Hint: the term is described in Activity 5.)
_____________________________________
7. a. What was the effect of atropine on heart rate? ___________________
b. The text states that atropine blocks the effect of acetylcholine. Do your results
support this? Explain your answer.
Note: A chemical that is an agonist enhances the effect of another chemical. A
chemical that is an antagonist inhibits the effect of another chemical.
c. Is pilocarpine an agonist or an antagonist to acetylcholine's effect on the heart?
___________________
d. Is atropine an agonist or an antagonist to acetylcholine's effect on the heart?
___________________
8. a. What is the effect of epinephrine on the heart? ___________________
b. Which branch of the autonomic nervous system does it imitate?
_______________________________
c. Is epinephrine is an agonist or antagonist to acetylcholine? _________________
d. How do these results compare to the observations you made during the movie in
the Endocrine System lab using the frog heart, epinephrine and ACh?
9. a. What is the effect of digitalis on the heart?
____________________________
b. Is its effect more similar to epinephrine or to acetylcholine? _________________
Ex 33A
1. About how long is the interval between the second heart sound and the following first
heart sound? _____________________ seconds.
2. a. Which pulse point had the greatest amplitude?
b. Which pulse point had the least amplitude?
c. How can you explain this?
d. What is your lab partner's average radial pulse rate? ______________
3. What is the formula for calculating mean arterial pressure?
4. Record data here from the sphygmomanometer.
First Trial:
Second Trial:
Systolic P ________mmHg
Systolic P_______mmHg
Diastolic P ________mmHg
Diastolic P ________mmHg
Mean Arterial P _______mmHg
(Show your work)
Mean Arterial P _______mmHg
(Show your work)
5. Venous Pressure.
Record peripheral venous pressure at rest here ________________
Record peripheral venous pressure during the Valsalva maneuver.____________
Explain the difference.