1. GERMAN-POLISH-RUSSIAN MEETING on Bacterial Carbohydrates Satellite meeting of the 20th International Carbohydrate Symposium, Hamburg, Germany RESEARCH CENTER BORSTEL SEPTEMBER 4-5, 2000 GENERAL INFORMATION Location Research Center Borstel Building "Reflektorium", Parkallee 29 Technical matters slide and transparency presentations as well as power point presentations Office of the meeting Building "Reflektorium", Parkallee 29 Bettina Brand and Maike Kohlmorgen phone: ++49-(0)4537-188-566 what for? Leave your slides in the break ahead of the session your talk is scheduled Any questions? Come and see us! (September 4-5) Accommodation Hotel Residence Krankenhausstr. 4 23795 Bad Segeberg phone: ++49-(0)4551-9650 fax: ++49-(0)4551-965400 or Hotel Teegen Heiderfelder Str. 5-7 23816 Leezen phone: ++49-(0)4552-290 fax: ++49-(0)4552-9169 or Hotel Stadt Hamburg Kurhausstr. 2 23795 Bad Segeberg phone: ++49-(0)4551-2210 fax: ++49-(0)4551-92186 Case of Emergency? Call Otto Holst on his mobil phone or Call Bettina Brand on her mobil phone 2 0171-4075678 0170-3335534 PROGRAM Monday, 4. September 2000 08:30 Otto Holst, Borstel Welcome address Chair: L. V. Backinowsky, Moscow 08:40 Keynote Lecture J. Weiß, DIONEX Corp. Carbohydrate analysis with anion-exchange chromatography and pulsed amperometric detection 09:10 N. E. Nifant’ev, Moscow Automated protocols to assay carbohydrates 09:30 A. S. Shashkov, Moscow Some aspects of application of NMR spectroscopy to the structural analysis of bacterial polysaccharides 09:50 G. V. Zatonsky, Moscow New band-selective 2D NMR experiments for structure elucidation of complex polysaccharides 10:10 F. V. Toukach and A. S. Shashkov, Moscow Computer-assisted structural analysis of polysaccharides and derivatives thereof 3 10:30 A. V. Perepelov, Moscow Application of triflic acid for selective cleavage of glycosidic linkages in bacterial polysaccharides 10:50 Coffee break Chair: V. N. Shibaev, Moscow 11:15 L. V. Backinowsky, Moscow Chemical synthesis of bacterial polysaccharides and their fragments 11:35 W. Szeja, A. Gamian, G. Pastuch, J. Wandzik and A. Sieron, Gliwice/Wroclaw Modification of bacterial oligosaccharides by glycosidation 11:55 A. Dawtjan, Rostock An efficient approach for glucuronidation of lactones 12:15 B. Nolting, Rostock Synthesis of the repeating unit of rhamnogalacturonan I 12:35 M. Brackhagen, Rostock Synthesis of human milk oligosaccharides containing fluorine labelled fucose 12:55 Lunch 4 Chair: Yu. A. Knirel, Moscow 14:00 T. N. Druzhinina and V. N. Shibaev, Moscow Biosynthesis of 2-acetamido-2,6-dideoxyhexoses, components of bacterial cell wall and capsular polysaccharides 14:20 T. N. Zvyagintseva, N. I. Shirokova, V. V. Sova, A. S. Scobun, and N. N. Besednova, Vladivostok Enzymatic transformation products of yeast -1,3-glucans. Biological activity 14:40 I. B. Naumova, Moscow Cell wall anionic carbohydrate-containing polymers in the classification of the order Actinomycetales 15:00 G. Kogan and H. Jennings, Bratislava/Ottawa Group B streptococcus capsular polysaccharides 15:20 N. A. Paramonov, M. Rangarajan, M. A. Curtis and E. Hounsell, London Structural characterization of the polysaccharides from Porphyromonas gingivalis 15:40 Coffee 5 Chair: A. Gamian, Wroclaw 16:05 P. Cescutti, Triest Structural investigation of exopolysaccharides produced by different clinical isolates of Burkholderia cepacia 16:25 N. A. Komandrova and S. V. Popov, Vladivostok Polysaccharides of the marine bacteria of the genus Pseudoalteromonas 16:45 S. Gabr, A. Hamed, H. El-Said and H. Hefnawy, Zagazig Chemical and physical modification of xanthan gum polysaccharide 17:05 B. T. Shawky, Cairo Bacterial alginate produced by Azotobacter vinelandii BTS 44 in pH-controlled batch fermentation 17:25 B. Kneidinger, Vienna Bacterial surface layer glycoproteins 17:45 Closing 18:00 Dinner 6 Tuesday, 5. September 2000 Chair: J. Radziejewska-Lebrecht, Katowice 08:30 Keynote Lecture B. A. Dmitriev, S. Ehlers and E. Th. Rietschel, Moscow/Borstel New concepts of bacterial cell wall physiology 09:00 T. Baasov, S. Du, H. Tsipori and V. Belakhov, Haifa Towards the development of novel antibiotics acting at the lipopolysaccharide biosynthesis 09:20 I. Helander, Helsinki Polycationic agents bind to the LPS layer of Gram-negative bacteria outer membrane with loss of the permeability barrier function 09:40 J. Rybka, A. Korzeniowska-Kowal and A. Gamian, Wroclaw Monitoring of substitution of Kdo in lipopolysaccharide by means of methylation analysis of LPS 10:00 W. Jachymek, J. Lukasiewicz and Cz. Lugowski, Wroclaw Applications of HR-MAS-NMR and MALDI-TOF mass spectrometry in studies of bacterial lipopolysaccharides 10:20 E. Katzenellenbogen, Wroclaw Structural studies on lipopolysaccharides from Hafnia alvei strains 10:40 Coffee break 7 Chair: U. Zähringer, Borstel 11:05 S. Fukuoka, K. Brandenburg, M. Müller, B. Lindner, M. H. J. Koch and U. Seydel, Takamatsu/Borstel Supramolecular structure of the lipid A fractions of lipopolysaccharide from Erwinia carotovora in relation to biological activity 11:25 Z. Sidorczyk, K. Zych, M. Kowalczyk and Yu. A. Knirel, Lodz/Moscow Determination of new serogroups in the genus Proteus 11:45 A. Rozalski, W. Kaca and Yu. A. Knirel, Lodz/Moscow Serological studies of Proteus vulgaris and Proteus mirabilis Oantigens 12:05 A. N. Kondakova, F. V. Toukach and S. N. Senchenkova, Moscow Determination of structures of the O-specific polysaccharides of Proteus 12:25 J. Radziejewska-Lebrecht, Katowice The complexity of the R-core region of Proteus lipopolysaccharides 12:45 K. Gwozdzinski and W. Kaca, Lodz Interaction of Proteus mirabilis lipopolysaccharides with human erythrocyte membranes, studied by spin-labelling method 13:05 Lunch 8 Chair: A. Rozalski, Lodz 14:00 V. L. L’vov, Moscow Characterisation of low-toxic and low-pyrogenic lipopolysaccharides of Shigella 14:20 B. Maczynska and A. Przondo-Modarska, Wroclaw Serological studies of Klebsiella lipopolysaccarides representing different O-groups 14:40 O. V. Bystrova, N. A. Kocharova, E. Katzenellenbogen and A. Gamian, Moscow/Wroclaw Structural studies of the O-specific polysaccharides of Citrobacter 15:00 P. Sanchez-Carballo, Borstel Role of the core oligosaccharide structures during Pseudomonas aeruginosa infections 15:20 G. M. Zdorovenko, Kiev Chemical and biological characterisation of lipopolysaccharides of phytopathogenic Pseudomonas 15:40 E. L. Zdorovenko, Moscow Elucidation of the O-specific polysaccharide structures of some pathovars of Pseudomonas syringae 16:00 Coffee break 9 Chair: Z. Sidorczyk, Lodz 16:25 E. V. Vinogradov, Ottawa The structure of lipopolysaccharides from Bordetella 16:45 O. Kooistra, Borstel Complexity of O-acetylation in the lipopolysaccharides of Legionella pneumophila serogroup 1 17:05 Y. A. Knirel, Moscow Mimicry of Lewis blood group antigens by the lipopolysaccharide of Helicobacter pylori 17:25 A. Choma, Lublin Structure of the O-specific polysaccharide of Mesorhizobium hualanii IFO 15243T 17:45 T. Urbanik-Sypniewska, Lublin The O-specific polysaccharide of Mesorhizobium loti HAMBI 1148 18:05 L. Varbanets, Kiev Ralstonia solanacearum lipopolysaccharides: structure-activity relationships 18:25 Closing 19:00 Bufet dinner 10