OCEAN COUNTY COLLEGE
Science Department
Course Outline and Syllabus
Spring 2013 Semester
Course Number and Title:
BIOL 232L-02, BIOL 232L–06
Dr. Robert J McNeil, Instructor
Course Hours – 0.0 Contact Hours– 1.67
Disclaimer: Changes to this course outline/syllabus may be announced at
any time in class or by e-mail or on Ocean Cruiser.
Class Meeting Schedule:
2013
Spring
Semester
BIOL-232L-02
LabMicrobiology
2013
Spring
Semester
BIOL-232L06
LabMicrobiology
Ocean
County
College
Campus
01/29/2013 05/14/2013
Laboratory
Tuesday
02:00PM 03:40PM,
William T.
Hiering Science,
Room 227
Ocean County
College Campus
R.
McNeil
0 / 18
Closed
01/25/2013 05/10/2013
Laboratory Friday
02:00PM - 03:40PM,
William T. Hiering
Science, Room 227
0.00
R.
McNeil
0 / 18
Closed
0.00
Catalog Description:
Title
Lab-Microbiology
Course
Section
Number
BIOL-232L-02, BIOL-232L-06
Description
This is the lab component of on-campus Microbiology. All
students taking on-campus sections of Microbiology must
register in both a lecture (BIOL 232) and a lab (BIOL-232L).
Registration in a lab component is NOT required for Distance
Learning and Off-Campus sections.
Credits 0.00 CEUs
Start Date
Level
29 January 2013 Section02
14 May 2013 Section02
End Date
25 January 2013 Section06
10 May 2013 Section 06
UG - Undergraduate
PREREQUISITES: BIOL 120 or BIOL 131 or BIOL 162
COREQUISITES: None
Maximum Class Size: 18 (Each Section)
Justification:
This course is required by the programs of study as outlined and approved for
students in the nursing and/or allied health department.
Contact Information:
Office Hours: Before or after class or by appointment. I have no
campus office.
Other Meeting Times: To arrange a meeting with your instructor
please contact your instructor directly or by e-mail (preferred,
robert_mcneil@portal.ocean.edu) or contact the Department of Science
and Engineering Phone: (732)-255-0400, x 2178
E-mail: Official course communication is to and from your Ocean
Cruiser e-mail. (firstname_lastname@portal.ocean.edu) (all lower
case)
E-mails must include your course and section number in the title
(subject) of your message or it may be deleted. Also include the
subject of your message. Do not send me e-mails with questions that
can be answered in class or in the syllabus. Contact another student to
find out what you missed, announcements, etc. if you are absent from
class.
Ocean Cruiser: All students enrolled in this class will be required to use
Ocean Cruiser, a web-based course enhancement tool/personal organizer.
You can access Ocean Cruiser from the Ocean County College Web Page
(www.ocean.edu). Once you have accessed Ocean Cruiser, place this link into
your Favorites folder. Login instructions are located on the introductory
page of Ocean Cruiser (you can also call for assistance [732-255-0400], if
you need it). Once you have logged in, you will find a link to our course page
– our course page contains important information, including the course
syllabus, email access, and a shared files area where I post lecture
presentations, and lab exercise supporting information, etc. As a rule, you
should access Ocean Cruiser and your OCC e-mail at least once per day.
All email sent to me should be sent via your Ocean Cruiser e-mail
address not from your personal e-mail address and should have a subject
title, course and section number, otherwise it may be deleted. Routine
questions should be asked in class, not by e-mail.
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Dept Office and my mailbox: Hiering Science Building Room 136 (Tom’s
River).
Contact Phone: 732-255-0400, extension 2178 (Dept. 0ffice) for
departmental (Biology) questions.
Textbook/Laboratory Manual/Materials and Supplies:
Required text (on Cruiser shared files):
Microbiology Laboratory Manual, BIOL 232
by Dr. Catherine Murphy
Recommended Text (from lecture), any one below:
MICROBIOLOGY:SYSTEMS APPROACH
Author
ISBN
COWAN
9780073522524
This item is the New or Used Textbook Only.
MICROBIOLOGY:SYSTEMS APPROACH-W/ACCESS
Author
ISBN
COWAN
9780077471941
This item is the Textbook bundled with the Connect Plus Card.
EBK MICROBIOLOGY:SYSTEMS APPROACH (CS)
Author
ISBN
COWAN
9780077290610
eContent Download (No Shipping)
See REQUIREMENTS before purchasing
eBook Format :
This item is an Ebook. This selection is a down-loadable book, before purchasing this selection please see the
econtents requirements above. Purchase either the Ebook or the textbook not both.
Required Supplies: Unlined drawing paper and no. 2 pencils, pen and
note-taking paper for lab.
How to Study for This Course:
1. Spend at least 2 hours out of class study time for each 1 hour in
class time which equates to 3.34 hours out of class study time per
week for our 1.67 hour lab.
2. Seek campus tutoring assistance and/or accommodations
early in the semester if you feel you need it. (see Campus
Resources and Services and Statement of Accommodation
below).
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3. NO MAKEUPS WITHOUT A DOCUMENTED (example:
Doctor’s note) excuse and makeups are not guaranteed.
4. Assignments must be completed by the due date, no later.
Reading Your Lab Manual:
1. Read the questions at the end of each lab exercise, if any. This will
help you to acquire a general overview regarding the contents of
each exercise.
2. Read the scheduled lab exercise procedures and relevant
videos/websites in the lab manual and the syllabus before lab to
help you understand it and to save time when lab begins.
SPECIFIC COURSE LEARNING OBJECTIVES
Students who successfully complete this course will be able to; (mostly objective h.
for this laboratory section):
a.
b.
c.
d.
e.
f.
g.
h.
Describe the major classes of organisms that infect the human body with
regard to general structure and patterns of infectivity.
Describe key elements of Host Parasite relationships.
Discuss the relationship between bacterial physiology and mechanisms of
disease pathogenesis.
Identify the major bacterial, viral, fungal and parasitic pathogens, and their
consequences in human disease.
Explain the general mechanisms of anti-microbial drugs and the nature of
microbial resistance to drugs.
Explain the concepts of virulence and virulence factors, and apply this
knowledge to the understanding of the spectrum of microbial disease.
Describe the major diseases that occur according to pathogens and by organ
system.
Understand general laboratory techniques and explain how they are used to
differentiate between different etiologic agents.
METHODS OF INSTRUCTION
Formal lecture and laboratory sessions; mostly laboratory for this section.
Videos/website readings are possible, time-permitting; or assigned as homework.
INSTRUCTIONAL MATERIALS / TECHNOLOGY NEEDS / HUMAN RESOURCE
NEEDS (PRESENTLY EMPLOYED VS. NEW FACULTY)
Text: An appropriate textbook will be selected.
adoptions.
4
Please see above for current
TENTATIVE TOPICAL OUTLINE (MOSTLY COVERED IN LECTURE)
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Introduction to Microbiology
Cell Structure and Function (Prokaryotes)
Cell Structure and Function (Eukaryotes
Microbial Growth
Energy and Enzymes
Introduction to Metabolism
Glycolysis and Fermentation
Kreb’s Cycle and Electron Transport
Control of Growth (Chemical and Physical)
Normal Microbiota
Control of Growth (Antibiotics)
Antibiotic Resistance
Antiviral and Antifungal Drugs
Principles of Disease & Virulence Factors
Diseases of the Skin and Eyes
Vector-borne Diseases
Diseases of the Respiratory System
Diseases of the Digestive System
AIDS
Instructional Delivery Methodology
Laboratory sessions will stress a hands-on/discovery approach to scientific
investigation. Powerpoint presentations or videos may also be presented.
Ocean County College Grading Scale:
All student assessment methods in this lab will be measured using the Ocean
County College grading scale. Final grades will be either PASS or FAIL. The
grading scale used to determine a final grade of PASS or FAIL is below. A
final average of 70-100%=PASS for the final grade. A final average below
70%=FAIL for the final grade. The lecture instructor will be provided with
the final % that you earned in this lab.
Student Assessment Methodology: (Calculated with a point system, total
points earned/total possible points = final %) but using the following
percentages breakdown.
Approximate Grade % Breakdown: 50% Lab Reports/Attendance, 25%
Unknown, 25% Final Exam/Practical
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Laboratory
Lab reports, quizzes, video attendance, laboratory examinations (practicals).
Lab reports, quizzes, and video attendance usually count 10-20 points each,
sometimes more. Lab practicals usually count 50-65 points, sometimes
more. Missing lab, lab quizzes, practicals, reports, videos, and other
assignments will result in a zero for those exercises.
Course Requirements:
If a student is absent for a test or laboratory session, it is the student’s
responsibility to contact the instructor before the missed class if possible to
make arrangements for a make-up session at the instructor’s discretion.
Attendance at all laboratory sessions is mandatory and may constitute part
of your final lab grade especially if you miss announced or unannounced
quizzes, videos or assignments. For a more detailed discussion on
attendance, please refer to the General Policy Statements section of this
document.
Examinations: Examination format will include multiple choice, matching,
fill-in, and true/false questions, which will test your knowledge of material
presented in lab, the lab manual, and lab items on Ocean Cruiser shared files.
All tests must be taken on the date scheduled except in case of an
emergency. In case of the above, the instructor must be notified in advance
that a test is going to be missed and an acceptable excuse must be provided
such as a doctor’s note. Acceptance or denial of the excuse is at the
discretion of the instructor. Lab practical exams are usually impossible to
makeup as are lab exercises.
Laboratory Requirements: The laboratory component of this course has
specific requirements. First, it is important to maintain a clean, safe working
environment; therefore the following safety rules must be observed:
1. No food or drink is allowed in the laboratory.
2. No sandals or open-toed shoes are allowed in the laboratory.
3. Hand washing and other hygienic methods must be employed to
ensure that there is no contamination to each other or to the
specimens.
4. Microscopes must be cleaned before they are returned to the
microscope cabinets; and they must be carried and returned
following approved procedures.
5. All waste must be disposed of in the appropriate receptacle.
6. The laboratory must be cleaned and all instruments returned to
their appropriate storage container before the class is dismissed.
6
7. Gloves and other safety apparel are to be worn during dissections
and when using chemicals/reagents.
8. NO MAKEUPS WITHOUT A DOCUMENTED (Doctor’s) note.
9. Assignments must be completed by the due date, no later.
The academic requirements of the laboratory are the following: Laboratory
reports: Drawings in pencil on plain white paper and answers to laboratory
questions as directed by the instructor. Label each report with name, date,
course number and section, laboratory topic title. Label all drawings and with
the microscope power (40X,100X,400X,1000X) that you used. All lab reports
must be typed, double-spaced, including figures. References must be cited.
Research paper format (outline) must be followed for experimental labs.
Laboratory Examinations and quizzes: Based upon information covered, and
work completed in the laboratory. Mostly with identification-type questions
from the lab manual, shared lab files on Ocean Cruiser, lecture text figures
and possibly live samples. Experimental labs will be tested based on an
understanding of the procedures and an understanding, knowledge, and
discussion of the results. Questions are usually multiple choice and truefalse. No makeup tests or makeup of lab exercises or videos will be provided
if you fail to notify and discuss your situation in advance or asap with the
instructor. No extra credit work will be made available to make up for a test
grade, laboratory or video.
General Policy Statements:
It is my responsibility as the instructor to create an environment that is
conducive to learning. This begins with commencing and ending class on
time. If you are late to arrive and/or need to leave early please do so in the
least disruptive manner. Take a seat close to the door, remove your coat
prior to entering or after leaving the classroom, have your notebook in hand
and/or pack up after you have left the classroom. If you are late for a lab
quiz you may have to wait outside until the quiz is over, and hopefully be
able to take it later, no guarantee. Please do not conduct personal business
during class – this includes all types of personal conversations (live, text
messaging, phone calls, etc.). In fact please turn all electronics off. If you
miss a class ask another student what you missed before the next class. I
may have made important announcements. Do not e-mail me to ask for this
information. I have 100’s of students in class thus do not have time to
answer frequent and numerous routine e-mails, especially pertaining to
questions that can be asked in class. Save all routine questions for lab class
time, not by e-mail. Do e-mail me if you will miss class because of sickness
or other emergency which may have to be verified to make up missed work.
Attendance: During each class meeting I will distribute an attendance sheet
please place your initials next to your name. The attendance sheet may also
7
be circulated at the end of class, so do not leave early. Some labs will be
graded based on attendance and participation. Do not sign for another
student. This would be academic dishonesty.
Course withdrawals: Anyone who withdraws from this class must acquire
the appropriate paperwork from the Registrar’s Office to “officially
withdraw” by the withdrawal deadline. Do not withdraw without talking to
me first. I may have suggestions to help you succeed in the course, such as
seeking tutoring help. (See Campus Resources and Services below.) Nonattendance does not count as an official withdrawal and will result in a grade
of “F” for this course.
Civility Statement: Ocean County College defines civility primarily as the
demonstration of respect for others, basic courtesy, reciprocity (treating
others as we wish to be treated), and behaviors that create a positive
environment in which to learn and to work.
The Trustees of the College and the College Administration set the tone for
civil behavior through their professional conduct and through their
leadership of the institution. All members of the college community create a
positive environment characterized by considerate and principled conduct.
While no civility statement can guarantee considerate and principled
conduct, the values set forth herewith represent institutional ideals and
should serve as guide posts:
1.
2.
3.
4.
5.
6.
Respect for the work of all persons
Courteous discourse (oral, verbal, non-verbal and electronic)
Honest interactions and utterances
Fair and just treatment
Integrity and keeping promises
Commitment to the community college philosophy: Access, transfer,
career preparation, workforce development, partnering, and
community outreach.
http://www.ocean.edu/welcome/mission_vision.htm
7. Open professional communications
8. Diversity, professionalism, and collegiality
9. Free expression of views without meanness or a desire to do harm
Tolerance of differing points of view
10.
Zero tolerance for any forms of cyber stalking, cyber bullying, or
cyber sexual harassment (see the Attorney General's letter)
11.
A culture of honor that enhances our students' ethical and moral
development and clearly communicates and consistently adheres to the
definitions of and sanctions for academic dishonesty
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These ideals are consistently modeled by those in leadership positions-in the
administration, staff, faculty, and student body-and should provide direction
for all members of the college community.
Academic Honesty: “Academic dishonesty includes, but is not limited to
plagiarism, cribbing and cheating on examinations, quizzes, class projects or
papers. Any student aiding another student in any form of dishonesty is
considered equally guilty.” OCC Student Handbook, pg. 35.
Cheating is not acceptable and will result in a grade of 0 for that test or
assignment and will be turned over to the appropriate College source for
disciplinary action. Do not turn in lab reports that are duplicates of that of
another student. That is plagiarism.
Please review policy #5180 in the student handbook for the penalties
associated with plagiarism and cheating.
Campus Resources and Services: Tutoring is available [a] in the Writing
Center for writing assignments in all subject areas, not just English courses,
and [b] in the Mathematics Tutoring Center. Tutoring information for all
other subjects can be found on the Tutoring page on the college website. In
addition, Study Strategy Seminars are scheduled each week and are posted
on the college website under “Academics.” More information on college
services can be found by using the A-Z index on the college website (for
example, under “T” for Tutoring or under “S” for Study Strategy Seminars).
Statement of Accommodation: If there is any student in this class who has
special needs because of learning disabilities or other kinds of disabilities,
discuss this with a staff member in the Center for Academic Excellence prior
to course work for which you may need accommodation and then provide me
with documentation to meet your special needs. You do not have to tell me
what your specific disability may be, only what accommodations you may
need verified with a completed form from the Center for Academic
Excellence.
All individuals should not assume that anything received, sent, or stored in
this course or in any course is private. Students’ written work, assignments,
and test results may be used anonymously for college assessment purposes.
Course content, support materials, and communications (including chats,
discussions, emails, and any other forms of communication) may be used for
quality assurance purposes by authorized college administrators.
Disclaimer: Changes to this course outline/syllabus may be announced at
any time in class or by e-mail.
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Important Notes
Ocean Cruiser is the official email communication for students at OCC.
(firstname_lastname@portal.ocean.edu)
Failure to pay for this course may result in your being dropped for nonpayment.
Only registered students are permitted in class-no parents, children, pets,
friends, etc.
Lab Schedule Spring 2013 Dr. Robert J McNeil
Course:
Fri06,Tue02
BIOL 232 - Micro Biology
Date
Experiment/***SUBJECT TO CHANGE****
Jan 25,29
Introduction: See video/website 1 below.
Orientation and Lab Safety: Manual-p.3.
Feb 1,5
Microscopy (Part 1) Manual-p.4-8;Text-p.11-15,66-71. See Handout 1 below on
Cruiser Shared Files.
http://www.youtube.com/watch?v=f7KlFSgdUGU&feature=relmfu – How to
use a microscope and oil immersion video.
Feb 8,12
Microscopy (Part 2) Microbes in the Environment: Manual-p.9-11. See
Handout 2 below on Cruiser shared files.
Preparation of Culture Media; examine plates from last week. Manual-p.17-19.
See Handout 5 below on Cruiser shared files. See video/websites 3,4 below.
Feb 15,19
Feb 22,26
Smear Prep; Simple Stain; Aseptic Technique; Aseptic Transfer Techniques
(streak plate only) (see video 2 below): Manual-p.12-14. Microbial Smear Prep
(see video 14 and website 15 below) and Simple Stain: Manual-p.15-16. See
Handouts 3 and 4 below on Cruiser shared files.
Mar 1,5
Intro to Unknowns: Manual-p.20-22. Differential Stains (Gram Stain)
Manual-p.23-24. See video/websites 5,16 below. Cultural Characteristics
(innoc of broth, slant, plate using UK), Manual-p.25-27 See Handouts 6,7,8,9
below on Cruiser shared files. See video/website 6 below.
10
Mar 8,12
Cultural Characteristics (interpretation): Manual-p.25-27. See Handouts 8,9
on Cruiser shared files. Endospore Stain-p.24. See video/websites 7, 8 below.
Motility (TBA)See video/websites 9,10,11,12 below.
Biochemical & IMVIC (coliform) Tests (Innoculations): Manual- p.28-33. See
Handout 10 on Cruiser shared files. See video/website 13 below.
Mar 15,19
Mar 22,
Apr2
Biochemical IMVIC Assessment; ID of UK; Pathogenic Microbes. Manualp.28-33. See Handout 10 on Cruiser shared files. Mar 25-31 Spring Break
Apr5,9
Sensitivity Tests- Antibiotics and Disinfectants (Innoculations): Manual-p.3439. See Handout s 11,12 below on Cruiser shared files. See video/websites
17,18 below.
Apr12,16
Sensitivity Tests- Assessments: Manual-p.34-39.
Apr19,23
Food Testing (Innoculation): Manual-p.40-45.
Apr26,30
Food Testing (Evaluation); Pathogenic Microbes Due. Manual-p.40-45. See
video/website 19 below.
May3,7
Lab Final
May10,14
Review of Lab Final; Videos.
Academic Calendar Spring 2013
Classes Begin
Wednesday, January
23, 2013
No Classes (Holiday)
Monday, February 18,
2013
No Classes (Spring Break)
Monday, March 25Sunday, March 31,
2013
Last Day of Classes - FOLLOW
Wednesday, May 15,
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MONDAY SCHEDULE
2013
Commencement
Thursday, May 23,
2013
Spring Quick Term 2013
Classes Begin
Wednesday, February
27, 2013
No Classes (Spring Break)
Monday, March 25 Sunday, March 31, 2013
Last Day of Classes - FOLLOW
MONDAY SCHEDULE
Wednesday, May 15,
2013
Microbiology Laboratory Manual, BIOL 232
by Dr. Catherine Murphy—Print it out from Ocean
Cruiser shared files and bring to every lab. It will not be
handed out.
Lab Handouts (see Ocean Cruiser Shared Files) Print
them out ifnecessary from Ocean Cruiser shared files
and bring to the appropriate lab. They will not be
handed out. Some may be the same as the lab manual
pages.
1)Use_and_Care_of_the_Microscope.pdf
2)Where_are_microbes.pdf
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3)Aseptic_Transfer_Techniques.pdf
4)Smear__Simple_Stain.pdf
5)Culture_Media.pdf
6)Unknown_data_sheet.pdf
7)DIFFERENTIAL_STAINS.pdf
8)Cultural_Characteristics_of_Microorganisms.pdf
9)ANAEROBIC_GROWTH.pdf
10)Biochemical_Tests_for_the_Identification_of_Bacter
ia.pdf
11)Antibiotic_Sensitivity_Test.pdf
12)Disinfectants_and_Antiseptics.pdf
13)ELISA.docx
Videos and Websites (extra reading or as assigned);
Others may be added:
http://www.youtube.com/watch?v=f7KlFSgdUGU&feature=
relmfu (MicrobiologyTechniquesWebsite-Excellent)
1.Microbiologist Video:
http://www.youtube.com/watch?v=KpLKi-b3xl8
2.Aseptic Transfer Techniques:
http://www.youtube.com/watch?v=P3oADI67i8k&feature=r
elated
13
http://www.youtube.com/watch?v=7RLtNC30Bj8
http://www.youtube.com/watch?v=zd0iVJrQwyY
3.Preparation of Culture Media:
http://www.sigmaaldrich.com/analyticalchromatography/microbiology/learningcenter/theory/media-preparation.html
4.Preparation
of media and cultures:
http://www.microbiologyonline.org.uk/teachers/preparationof-media-and-cultures
5.Differential Stains for Identifying Bacteria:
http://suite101.com/article/differential-stains-for-identifyingbacteria-a234529
6.Identifying Microorganisms by Cultural Characteristics:
http://www.austincc.edu/microbugz/cultural_characteristics.
php
7.Endospores: http://en.wikipedia.org/wiki/Endospore
8.Endospore Stain and others:
http://www.austincc.edu/microbugz/handouts/Stain%20prot
ocols.pdf
9.Bacterial Motility:
http://en.wikipedia.org/wiki/Bacterial_motility#Movement
10.Bacterial Motility:
http://www.youtube.com/watch?v=zlFRJftA2bU
11.Unknown A Bacteria--Motility Test:
http://www.youtube.com/watch?v=DM9_N4dXYog
12.Images for Bacterial Motility Tests:
https://www.google.com/search?q=bacterial+motility+
tests&hl=en&prmd=imvns&tbm=isch&tbo=u&source
=univ&sa=X&ei=i7pEUKi3NMy10QGDtoGgDQ&ved
=0CDcQsAQ&biw=1600&bih=799
14
13.IMVIC Tests:
http://en.wikipedia.org/wiki/IMViC
14.Microbial Smear Preparation:
http://www.youtube.com/watch?v=5TmyxFvo9x4&feature=r
elated (good)
http://www.youtube.com/watch?v=uPveNOnmQxI&feature=relmfu
(isolation of bacterial colonies)
http://www.youtube.com/watch?v=SLkipIg4WRg
15.The Smear and Simple Staining:
http://www.youtube.com/watch?v=2KDT82koX7w&feature
=relmfu (simple smears and stains-good)
http://www.youtube.com/watch?v=2EykxVWGFGc&feature=relmf
u (simple stain?-good)
http://www.professorcrista.com/files/stuff_that_stays/lab_06
smear_simple_stain.pdf
16.Gram Stain Technique:
http://amrita.vlab.co.in/?sub=3&brch=73&sim=208&cnt=2
17.Antibiotic Sensitivity Testing - Establishing the Minimum Inhibitory
Concentration: http://www.rapidmicrobiology.com/test-methods/AntibioticSensitivity-Testing.php
18.antibiotic susceptibility testing
by disk diffusion:
http://www.youtube.com/watch?v=_ujEXCXj4KE
19.Bacterial Counts - Quantitative Analysis of Microbes
http://biolabs.tmcc.edu/Micro%20Web/BacterialCount
s.pdf
20.Enzyme-Linked Immunosorbent Assay
(ELISA):
http://www.youtube.com/watch?v=RRbuz3VQ100
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