Midterm Exam – AP Biology Lab Review
Name:______________________________________ Date:___________ Period:____
The following questions are desgned to help you review AP Biology Labs 1-7 and 11-15
in preparation for your midterm exam.
AP Lab 1 Osmosis and Diffusion
Draw an onion cell as it would react to distilled water and how it would react to 15% salt
solution.
Onion cell in distilled water
Onion cell in 15% salt solution
A student set up the following experiment using potato cores. She determined the mass
before and after soaking in the labeled solution for 24 hours. They then calculated the %
change in mass. Next to the data table, make a graph of the data.
% Change
Distilled
15
0.2M
1.0
0.4M
-15
0.6M
-20
0.8M
-25
1.0M
-30
Answer the following questions concerning this data.
1. Use your graph to determine C which is the molar concentration of sucrose that
produces equilibrium.
2. Calculate the solute potential
Ψs = -iCRT
3. If the beakers are exposed to the atmosphere and the temperature is 20° C,
calculate the water potential?
AP Lab 2 – Enzyme Catalysis
Graph the following data and answer the
questions that follow.
Time
mL of H2O2
used 10º C
mL of H2O2
used at
35º C
10
0.3
1.5
30
0..6
3
60
0.9
4.2
120
1.2
180
1.5
4.5
360
1.8
4.5
4.3
1. Read over your AP Lab 2. What is the name of the enzyme and what is the name of
the substrate?
2. How does the lowering of temperature affect the rate of the reaction?
3. In this lab sulfuric acid was used to stop the reaction at the given time. Explain how
sulfuric acid works to stop the reaction?
4. What would you predict would happen is the enzyme was boiled prior to using it in
this experiment? Explain your answer.
AP Lab 3 Cell Division
1. Compare and contrast mitosis and meiosis.
2. Examine the asci below and calculate the % crossed over and map units.
AP Lab 4 Plant Pigments and Photosynthesis
1. What is the function of Chlorophyll a?
2. What are the functions of accessory pigments in plants?
3. Why do most plant leaves appear green?
Students performed an experiment using chloroplasts and DPIP as you did in AP Lab 4
using different wavelengths of light at equal light intensities. Their results appear below.
Graph their data and answer the following questions.
Time
in
min.
%Transmittance
in Red light
%Transmit
tance in
Green light
5
25
25
10
30
26
15
35
27
20
40
28
25
45
29
30
50
30
1. Explain why the percent transmittance increases with the addition of chloroplasts
over time.
2. How does the rate of photosynthesis compare between chloroplasts in the red light
compared to the rate in the green light.
3. Offer an explanation for the difference in rates.
AP Lab 5 Cellular Respiration
Several students tested the production of CO2 in crickets at two different temperatures.
Graph the data and answer the questions that follow.
Time in
seconds
10 º C
ppmCO2
35º C
ppmCO2
0
350
350
30
355
400
60
360
450
90
365
500
120
370
550
150
375
600
1. What is the rate of cellular respiration at 10 º C?
2. What is the rate of cellular respiration at 35º C?
3. Explain why the rates are different at the two temperatures.
4. Explain how the results would differ if an endothermic animal such as a mouse were used
instead of a cricket.
AP Lab 6 Gel Electrophoresis and Colony Transformation
1. Diagram and explain the process of Colony Transformation
2.
Diagram and explain how the process of gel electrophoresis is used to determine
the number and sizes of DNA fragments in a mixture of DNA fragments.
AP Lab 7 Genetics of Drosophila
AP Lab 11 Animal Behavior
AP Lab 12 Primary Productivity