CELL RESPIRATION-Let’s use those enzymes people!!
BEFORE YOU BEGIN!!! Pre-lab?’s:
1) Define cellular respiration. (This is what heterotrophs do-yeah, look that word up too as
needed!) 
2) Where do the raw materials your cell converts come from?
3) What waste product is produced during cell respiration?
4) What will your cells use as catalysts in cell respiration?
5) Do your best to fill this sentence in: Heterotrophs convert ______ energy into ______
energy for cell use.
6) What do enzymes have to do with cell respiration?
QUICK INQUIRY LAB: Decide who the recorder is and who is the guinea pig…
1) From the center lab island obtain a white cup, a straw and a graduated cylinder.
2) To the cup, add about 20 mL of bromothymol blue. (BE sure to not over add!!) .
3) Complete the data table below, you will be recording the guinea pigs data:
BEAKER
GP TIME (seconds) FOR COLOR CHANGE TO OCCUR
BEFORE EXERCISE
AFTER EXERCISE
4) The guinea pig must be able to get their cardiovascular system elevated through brief,
intense exercise today in lab. (So if you want, change roles now!) Recorder, take out your
device so you have a way to time this activity.
5) While at a state of rest, and when the recorder is ready, the GP GENTLY blows directly into
the solution in the cup with the BTB solution, (using the straw) while the recorder times how
long it takes for the solution to turn yellow to yellow green. (It might take some intense
blowing!) Do not stop until you get a color change! Be sure to start the time as soon as the
person starts exhaling and stop the timer as soon as you get a yellow to yellow green color!
6) Record that time in the correct box in your data table.
7) Have that same person perform a physical activity for 3-5 minutes. You can run in place, run
up and down the outside stairs, do stair steps using a desk seat. You can run around the
upstairs too as long as you don’t get loud and disrupt learning in other classrooms! Just be
sure to have the person exude enough energy to raise their cardiovascular levels.
8) While the GP is out running around like crazy, recorder, you need to dump the used BTB
solution into the sink and rinse with lots of water and set up the BTB again. You can only use
BTB once! Have the set up IMMEDIATELY ready to go as the GP returns!
9) As soon as the person is done elevating their cardiovascular rate, he or she will perform the
same procedure as explained in step 5.
10) Record data in correct box in your data table.
Post-lab ?’s:
1) What is the BTB an indicator for?
2) Therefore, make a general statement about what your data shows.
3) Think about basic cell process and add now where that substance that BTB reacts with is
coming from. (What causes the data?)
4) As a person exercises, what gas is needed in higher quantity? What gas is produced in
higher quantity?
5) As you exercise, cells are performing more work. What molecule is needed to do work?
What happens to that molecule as it is “used”?
6) The more work your cells do the more energy is spent. That said; think of 3 specific cellular
activities that require ATP, in order to be performed.
7) Write a general equation to show cellular respiration. (Hint you’ll find it on page 134 of the
text)
8) Using the terms oxygen, carbon dioxide, glucose, ATP, energy and cellular respiration at
least once in your answer, explain why exercising causes your body to produce CO2 at a
greater rate than when you are resting.
9) Review the relationship between catalyst and substrate and then predict whether there is
one or more than one enzyme involved in cell respiration and justify your answer?