94- m
PUBLISHEDBY WATERSCHROMATOGRAPHY DIVISION, MILLIPORECORPORATION
VOL. 1, NO. 1
SUMMER 1989
3-"
l
t
New PowerLine Based Automated System For
Dissolution Control: Waters 610/600E PowerLine
System for Dissolution Testing
.............................
• IN THIS ISSUE
NewPowerCine
Based
Automated
HPLC
System
for
DissolutionControl...
Introduction
four solventgradient system(functionIn 1987Watersfirstintroducedan
ally equivalentto the 600E)with the
automateddissolution/HPLC(High
addition of a gradientvalvestation,
PerformanceLiquidChromatograph)
ThesePowerLinemodulesare a new
interfacebasedon the 840 Data and
way to integratethe latesttechnoloChromatographyControl Station.This
giesof today's HPLCsystemcomposystemimprovesthe efficiencyof the
nentsintoa combinedcentralized
dissolutionlab by transferringsamples
controller,providingcompletesystem
directly fromthe dissolutionteststation
communicationand systemparameto the HPLC,analyzingthosesamples
ter documentation.Thisnew approach
and documentingthe results,
fromWaters providespower beyond
In March of thisyear,at the 40th
that of stand-alone HPLCcompoAnnual PittsburghConferenceand
nents.Additionally,PowerLineHPLC
Exposition,Watersannounced the
systemscan beconfiguredwith either
incorporationof dissolutionacquisian integrator,or a chromatography
tion software in the PowerLine 610
workstationusingWatersMaxima
is.ocraticand the 600Egradientsol, or ExpertEase'" software.
•
vent deliveryand systemcontrol
ThePowerLineAutomated
modules.The610isocraticPowerLine
Dissolution/HPLCsystemconsistsof a
systemmodulecan be upgraded to a
600Eor 610PowerLinemodule (Rev.
'
.
I
2.0 Software),a 484TunableUV/VIS
Absorbance detector,a WISP'" 712D
TM
TM
•
•
•
"
_'-"
_
i
,
z
i
DissolutionTestingRequiring
HighSensitivity
Analysis...
TheWaters845ChromatographyDataStation...
.WatersExtendsitsFamilyof
HPLCAutoInjectors...
- ....................................
1-3
4-5
5-6
7
sampleprocessor,and a Hanson
TransferController (TC)*which transfersdissolutionsamplesfromthe dissolutiontestapparatus directlyintothe
active712Dsampleprocessor.
Howthc PowcrI,ineSvstcmfor
:Dissolution "l_sting _,brks
Thefollowing operational description
isapplicable to eitherthe 610or the
600EPowerLinesolventdelivery and
systemcontrolmodules.
Dissolutioncontrolisaccessedfrom
the AutoinjectorSetupscreenof the
600Econtroller.When enabled,the
600Ecoordinatesthe transferof dissolutionsamplesintothe active712D
autosamplercarouselwhile performingchromatographyon previously
collecteddissolutionsamplesor
°1 Ic_30n ResecrcP (_'Cl_O'<:hOr:
calibration
_,;8"0 Vor,oI Ave"ul!
standards.
Chcrswor'h,
CA "2"3:
WatersPo;verLineD_ssolutionSystemincluding
Waters600EGradient PowerLineModule,
Waters484 detector,the WISP712D,and the
Hanson TransferControlle_(HansonSR-2
DissolutionBothcourtesyof ttanson Research
Corporation.)
The Hanson Transfer Controller
Through intelligent software rou-
(TC), activated by the 600E, removes
samples from the dissolution bath at
times specified in the Dissolution Table
editor. Autoprobes, equipped with
5-10 micron filters, are lowered simul-
tines and advanced sample handling
techniques, the Waters Automated
Dissolution/HPLC system provides
greater confidence in sampling and
analytical results. Should verification
taneously into each of the dissolution
vessels at the specified sampling time.
Excess filtered sample iswithdrawn
into six volumetric cylinders under
vacuum. Each of the six cylinders have
a liquid leveling rod to determine the
Waters600EPowerLine
ModuleAutoinjector
SerupScreen
Editor
allowsyoutoactivate
dissolution
software.
Inthepharmaceutical
indus-
of specific sample data be necessary,
identified samples can be re-injected,
since all dissolution samples are
retained until the analyst approves all
the data.
sample volume that will be dispensed
into the 712D autosam#er carousel,
try,it is commonto periodicallyruncalibration
standardsaftera predetermined
numberof
Combined l)i£_olL_d(_n
"rc_(ingand
Canton( t:niformit)' for Quail[)' Control
Using positive pressure, the TC delivers the excess sample back into the
dissolution vessels, simultaneously
backflushing the autoprobe filters,
The remaining measured and filtered
sample isthen dispensed into empty,
capped one ml vials inside the active
712D sample processor,
Dissolution sampling times are
entered in the Dissolution Table editor
of the 600E Controller according to the
featureofhthe600E
samples
avebeenanalyzedA
PowerLine
control
convenient
module
istheAutoStandards
routine,whichallows
theusertoprogramstandardvialinjections
betweena setnumberofsamplevials,
In addition to dissolution analyses,
content uniformity determinations
are also routinely performed in the
pharmaceutical laboratory Content
uniformity determinations are usually
performed separately by HPLC techniques, while dissolution results are
more often based on spectrophotometric techniques. Waters Automated
Dissolution System lets the pharmaceutical chemist combine dissolution
protocol specified by the dissolution
test. The injection sequence consisting
of samples and standards, and chro-
testing and content uniformity determinations using a single automated
method.
matography run times are entered in
the Program Methods editor,
The 600E dissolution software
Waters600EDissolution
Tablescreeneditor
allowsentryofsamplingtimes_
Theautomated
dissolution
systemuses:he96violcarouselin
In combining these two tests, three
criteria must be met. First,two dissolution baths must be used. Most dissolu-
determines whether an H PLCinjection
can be made without interfering with
the712Dautosampler
toaccommodate
the
many
samplestaken
duringo
dissolution
test.
The
D_ssolution
Table
editorprovides
theuser
the acquisition of dissolution samples,
If it can, the HPLC injection is campleted and the 712D sample carousel
is rotated to the correct position to collect the dissolution samples. On the
other hand, if the system determines
that it cannot make the next scheduled
injection before the next set of dissolution samples are acquired, it interrupts
the injection sequence to rotate the
712D carousel to the correct dissolution sample acquisition position. Once
dissolution samples are acquired, the
carousel is rotated back to the position
it was in before the sample transfer
withdocumentation
of sampletimesandvial
locationofdissolution
samples. Typically, the
firstsixpositions
of thecarousel
arereservedfor
multilevel
calibration
standards,
tion tests require that only six samples
be analyzed; however content uniformity determinations are usually performed on a minimum of ten samples,
necessitating the use of a second dissolution bath (six additional samples).
Second, content uniformity determinations must be performed when
the active ingredient(s) are 100%
dissolved.
Third, the same HPLC method must
be capable of analyzing dissolution
samples as well as performing content
uniformity determinations. If all three
of these criteria are met, content uniformity determinations can be made
utilizing either the same samples analyzed for the dissolution test (if actives
are known to be completely dissolved), or samples withdrawn from
the dissolution test vessels at a later
took place and the injection sequence
continues. In all cases, the 600E software prioritizes dissolution sample
acquisition over that of a sample or
standard injection,
There are cases ,where it is possible
that the samples scheduled to be
injected into the H PLChave not yet
been acquired. This may happen in a
situation where the dissolution sampiing intervals are hours apart and the
chromatography is programmed at
minutes per injection. In this case, the
dissolution software recognizes that
the samples scheduled for analysis
have not been acquired and will delay
injections from these vials until they
have been filled with dissolution
samples.
-2-
time point (if the last dissolution sampiing time is known to occur before the
actives are 100% dissolved).
_l
.
]
Todemonstrate the feasibility of
combining these two test procedures,
identical HPLC methods (See Figure 1)
were used for both the dissolution test
samples and content uniformity deter-
Figure 1: Waters 860 Netwo&ing
Workstation Chromatography
Sample Report of Timolide
Separation
.........................................
Header:
minationsfor the antihypertensive
productTimolide_.Timolidecontains
hydrochlorothiazideandtimolol
maleate at 25 mg and 10 mg per
tablet respectively
The Waters Automated Dissolution
System was used to control the sampiing of 12 dissolution test vessels,
i
Acquisition method
Units
COMBINED3
Processing
System
number
method
COMBINED3
I
Channel
Injection
1
1
Vial
Total
30
1
Run
time
Injection
volume
Acquisition
version
9.00
50 uL rain
840/6.21
Sample
rate
Mode
Processing
version
injections
i
i
l.O0 per
Analysis
Vl.2
sec
i
i
Description:
M404@295NM
ri rstPlot:
_3E ,,.,i_ _.__,,..,o,4,_,.,
i ch_,,_,_.,
_
,
....
•
55"
transfer the samples to the active 712D
autosampler, and analyze the dissolution sample and standard solutions.
In this case, the Timolide actives were
completely dissolved at the final disso-
i
_
i
,_,v s_"
4s4_
3s"
30"
_s
!
f
i
_
mity determinations were based on
the HPLC
lution
test time
results
point.
obtained
Content
fromuniter10 of
the 12 dissolution samples. Additional
content uniformity samples prepared
offline by accepted United States
l_'s"
_i
_-
Run time
Injection
Scale factor
Integration
Delay
Relative
composite drug assays on a single
automated system providing them
with the specificity and sensitivity demanded by today's drug formulations.
rate
amount
standard amt Analysis
Keyboardsof RemoteDevices Locked
PeakDetect Threshold 2S
The Waters PowerLine System for Dissolution testing brings a new dimension to dissolution analyses. Itsease of
use, versatility precision and reliability
surpass all other dissolution sample
assay techniques. It is the only online
dissolution HPLC system that performs
a complete drug product release
study. Now for the first time researchdissolution
'8 " " '
Peak
0.00
sec
Quantltation
by Area
Force Through
Zero is Disabled
10%
7.22
Reject
Absolute
693929
Area
25000
Peak
window
HeightInt
49907
BB
394845
18768
OFF
Amount
0.028
Intercept
0.000e+00
SS
0.011
O.O00e+O0
Peak
Name
Slope
Response
HYDROCHLORTN.
2. 516e+07 6. 93929e+05
TIMOLOL
3.647e+073.94845e+05
..............................................................
HPLC separation of Timolide active ingredients, hydrochlorothiazide
and tirnolol maleate art a Waters
_Bondapok C_u column. The mobile phase consisted of water, ocetonitrile, methanol and pt t 3.0
phosphate buffer (38:8:2:2). The separation was monitored at 295 nm as specdied in USP_0{I.
Table 1: Comparison of Manually Prepared Content Uniformity Sample Data to the Combined 10
Vessel
Dissolution
Data
.............................................
Hydr0chlorothiazide
Tablet
mg/ml
Tirnolol
Maleate
Auto
Tablet
mg/ml
Manual
Tablet
Auto
mg/ml
Tablet
mg/ml
9.93
9.92
1
2
25.05
25.38
11
12
24.53
24.58
1
2
9.88
10.01
11
12
The data in Table I indicates that the automated
3
25.10
13
24.62
3
9.80
13
9.78
method
45
6
7
8
24.93
25.03
24.83
25.23
25.05
14
15
16
17
18
25.23
24.78
25.34
24.61
25.50
4
5
6
7
8
9.81
9.97
9.74
9.98
9.85
14
15
16
17
18
9.99
10.00
10.20
9.63
9.79
outcome of the content uniformity test. From the
analysis of the means and variances by T-tests
and F-tests respectively, we conclude that the
manual method o{ sample preparation {or content uniformity could be substituted with the
9
10
25.18
25.88
lg.
20
25.21
2525
g
10
9.89
10.30
19
20
10.06
AVG
SDV
25.17
0.28
24.96
0.35
9.92
0.15
9.92
0.15
automated
RSO
1.11
1.42
1.51
1.52
sample
preparation
method,
but same chromotographic
method of onolysis),
gives a similar mean and variance indicating
that this method does not significantly
affect the
dissolution
method
without
see
20 sec
Area
Ret 4.78
Time
TIMOLOL
PeakWidth
per
min
Window
LC Results:
PeakName
HYDROCHLORTH.
1.00
3.00
Manual
(different
I
,
,
Sample
Sample
Internal
Mode
Single Point Calibration
Retention
Time Offset
profiles, content uniformity studies and
,-,,
9.00 min
50 uL
volume
(_OI]C_usJoI]
extended
/k
'6
conditions:
Pharmacopeia (USP)methodology
were manually loaded into the WISP
carousel for comparison with the dissolution samples (see Table 1).
ers can execute
ik
-'4 _
M_,',_te-_
_'2
signifi-
cant bias to the results........................................................
-3-
Dissolution Testing Requiring
Sensitivity Analysis
High
Thetrend in the pharmaceuticalindusFigure2: Waters860 Expert EaseSystemSuitabilityReport
try istoward morecomplexformula................................................................
tions,lower dosages, and more
System
Suitability
Report
from line
i of multi-method
SYSS
sophisticateddrug deliverysystems
Acquisitionmethod SYSSUZT Processin9
method
suchas controlledreleaseproducts.
Units
syst
..... bet
Channel
1
Vial
As a result,pharmaceuticalresearchInjection
2
Total
injections
Run time
15.00
min
Sample
rate
ers are moving away from the tradiInjection
volume
i00 uL
Mode
tional analytical spectrophotometric
Acquisition
version
840/6.21
Processing
Version
techniquesand are turningto HPLCfor
...._ vi,_ _,-.,.:t2ch,
_....
dissolutionanalysesand quality con__
trol.Theexcellentspecificityof HPLC
..... :2
_
makesit a moresensitiveand reliable
_0
¢
analytical tool than spectrophotometry Inaddition, the USPaccepts
HPLCas a method of analysisfor dis....
solutionsamples.
Dissolution
_
_
_r-.,
:.'_
_'J
]*_-_
4
0
by Papas,et el.) Theirmethod
involved dispersingthe tablet in 10ml
of a mixture of methanol and water
(80%/20%) • Thiscorrespondedto
working concentrationsof l00 p,g/ml
and 5 I-,.g/mlfor a tabletcontaining 1
mg norethindroneand 0.05mg ethinyl
estradiol
respectively,
Dissolutiontestingof oral contraceptiveswas modified by the USP2in
per
sec
Calibration
V1.2
,j
A
,.:
";
w
A
4
OI-alContraccptives
very low levels, hove been described
9
1.00
2
Testing of
Sensitiveand reproducibleHPLCseparations of 23 differentcommercial
oral contraceptivetablets, containing
norethindroneand ethinylestradiol at
S_rSS
1
1
Table
of
_
System
_ .....
_e
Minute__
Suitability
_2
_
Results
Sample
Na.... stdVial 1 ZnJZChartl
Sasel*ne
RMS noise
8.161671
Time
UNKNOWN Ret 2.44
UNK_;OWN
2.81
4.71
N_me
UNKNOWN
NORETHINDRUNE
ETRINYL
ESTRAD.
9.11
10.28
_ame
UNKNOWN
(minutes)
RetTime
2.44
UNKNOWN
2.81
drift
(uV)
-0.035435
(uV
/sec)
4013Height
385 Plates
-198Resolution 5235
107_
39n
7805
254
643
1.z72
0.n5
0. 582
Area
56495
23481
4420
1645
9575
10551
Tailing
2.779
21.282
Factor
1. 069
1.078
(data
points)
(seconds)
(seconds)
widtb:
baseline
S-sigma
half
height
24
25. 924
9. 808
15
UNKNOWN
4.71
9.11
ET.I*_ EST_D.10.28
..............................
13.
57
37
NURETHINDRONE
378
5.244
55.716
30.030
40
27.929
45.028
13.301
12.005
SystemSuitabilityReportof Oral ContraceptiveStandard Injection.Thesystemsuitabilityreport tara
single vial injectionprintsthe chromatogram and reports the USPparameterssuchas resolution,plate
counts,and tailing factors forall peaksidentified in the calibration table. In addition, a summary
report isalso printed for multipleinjectionsof standard vials,reporting means,standard deviations,
and relativestandard deviationsof area, height, retentiontime, tailing factor,resolutionand plate
count.
1988to reflectthe useof HPLCas a
method of analyzingdissolutionsampies.Usingthis method the tablets
are dissolvedin 600 mlof dissolution
estradiol.Thisrepresentsa 60-fold
dilution over the me'hodology originellydescribed by Papas.
Todemonstratethe sensitivityof
programmable,offering the chromatographer the ability to optimizethe
detectoroutput.
A standard solutionwas prepared
mediaand the samplesanalyzedby
HPLC.Theformulationdescribed
above would provide working concentrationsof 1.7I,zg/ml for norethindrone and 0.08_g/ml for ethinyl
Waters484 TunableAbsorbance
Detector,Waters Pharmaceuticallaboratoryperformed the dissolutiontest
outlined in the USPfor norethindrone
and ethinylestradio!tablets,
Inthis experiment,the uniquecapebilitiesof the PowerLineDissolution/
HPLCinterfacewere coupledwith
thoseof the484 absorbancedetector.
representingthe concentrationof a
typical oral contraceptivedissolution
sample.TheHPLCanalysisrequired
detection of thesecompounds at concentrationsbelow 1.7_g/ml and 0.08
i_g/mLfor norethindroneand ethinyl
estradiol respectively
The optics of the 484 detector
are
con-
trolledby the PowerLine600Esystem
controlmodule by meansof an lEEK488 camm unicotions bus.Wavelengthsand sensitivitysettingsare
-4-
:Popo:_, Andrew N, Marc} ...... Salvo'ore M .... d geloney.
M<hQcl F. Rop_d Delermrnahon
o{ Nore:hmdtorlu
and
F,_,,,,y,
Es:r,";d,o' ,,, O,al
phase I,qu,d
_85)
Contracept, ve foble/sby R.......
Chrorno:ogrophy.
LC, Vol 3 No .4. (Ap i
_._o,_:_,_,o_e
or.d Dh,nyl
Severlh
Suoplemenl
Fs,rad;ol
2813 !1988)
bble:s.
IJSP XXl.
d-
j
r
TheUSPprotocol suggests100p,I
injectionsizeswhich isequivalentto
170ng of norethindroneand 8 ng of
ethinylestradiol injectedonto the
HPLCcolumn.Given the poor spectral
characteristicsof thesetwo campounds,analytical requirementsdictarean HPLCmethod that can provide
a stable UVdetectorresponse,at high
sensitivity,when operating at 200 nm.
Dissolutiondata was reprocessed
on the Waters860 networkingcampurersystem.Accurateand reproducible integrationwas obtained on
peaks representingas little as a two
millivoltresponse,
Figure2 showsa systemsuitability
reportgenerated by the Waters 860
Networking Computer System,illustrating a typical separationof a syntheticallypreparedoral contraceptive
dissolutionsample usinga Waters484
absorbancedetector.Fivereplicate
injectionsof a standard solution
resultedin RSD'sof 2.5% for both
actives,below the 3.0% specification
setin the USPmonograph,
In addition, the systemsuitability
reportshowsthe theoreticalplatesfor
ethinylestradiolat 10,551(USPstates
7,000minimum)and the resolution
between the two activesat 2.8 (USP
states"i.5minimum).Thetailingfactor
forboth activeswas well below the
maximumof 2.0 describedin the USP
monograph.
The Waters 845 Chromatography
TheIdealChromatography _brkstation
for the Pharn_aceutical
l.al:x)ratory
TheWaters845 Chromatography
Workstationisone of the mostpowerful laboratory data handlingand control systemsavailable today It isa
quantitativetool that enablesthe pharmaceuticalchemistto measurearea
and/or peak height and to accurately
calculatethe trueamount of material
presentin samples,
"
Workstation
TheWaters845 combinesthe
power of the DigitalVAXstation2000
or the VAXstation3100DesktopCampurerwith the latestenhancementsof
WatersExpertEaseSoftware.The
845'swindow environmentenhances
the system'smultitaskingcapabilities,
WatersExpert Ease Software
WatersExpertEasesoftware isa
directaccess,menudrivensoftware
package optimizedfor gas, liquid,and
ion chromatographyapplications. (Thereisan optional
software package designed
for gel permeationopplications.)It automaticallycollects
data, integratespeaks,stores
methods,plotsin real-time,
,
runsstatisticsand generates
i
graphics and custom reports.
J
i
(;onclusion
Analysisof dissolutionsamplesby
chromatographictechniquescan put
a burdenon the HPLCsystem.Dilute
solutionsof activeingredientsmustbe
capableof being reproduciblyanalyzed.TheWaters Automated DissolutionSystemiscapable of providing the
userwith convenientmethodssetup,
variablesampleinjectionvolumesand
detectoroptions to meetthe demands
for high sensitivityanalyses.
------------------._
-
TheWaters ExpertEasesoftware
offersinternalor external,singleor
multi-pointcalibrationsup to 99levels.
Itallows linear,quadratic, cubic,
point-to-point and segmentedcurve
fits.Calibration curvesand regression
statisticscan be printed or plotted
forpresentationor documentation
purposes.
The845 offersdefault integration
parametersto meetthe needsof most
pharmaceuticalapplications and
integrationtimedeventsare provided for complexchromatographic
separations.
Waters Easy-Viewsoftwareallows
simplemousedrivenmanual integrationand chromatogram zooming to
seeand accuratelyintegrate eventhe
smallestpeaks.
Waters CustomReportManager
allows the userto tailor chromatographic reportformats,reportsequencesand the reportprint location.
Waters ExpertEaseCustomConvertcapability allows transferof valuable chromatographic resultsinto
RS-1,Lotus1-2-3%20/20 or ASCII
TM
Waters 845 Chromatography Workstation featurincj the VAXstotion31 O0pezsonal computer
from Digital Equipment Corporation.
-5-
formats for further statistical analysis .......
Complete methods documentation is
available for GMP/GLP purposes.
Waters I.AC/I-"lk'chnology
Waters Laboratory Acquisition and
Communications Environment
(LAC/E") module provides advanced
system control and data acquisition
capabilities to increase your laboratory's productivity:
The LAC/E module controls all
Waters pumping modules including
isocratic, single-pump gradient and
multi-pump gradients. It also controls
Waters family of WISP sample processors as well as Waters detectors (410
Differential Refractometer, 484 and
490 Absorbance Detectors). LAC/E
serial boa rd options offer the ability to
connect up to eight additional printers
offering a more organized reporting
structure for a multiple H PLC/GC
laboratory
Waters SAT/IN'" module provides
tWOchannels of high resolution A/D
with separate time bases to meet the
needs of capillary GC, as well as LC,
IC and GPC data collection.
Introducing Waters Expert
Ease
System Suitability Software Option
Waters Expert Ease System Suitability
software option provides the pharmaceutical chemist with critical HPLC
separation data needed to validate
i"
i
"x
\
Through
advanced
protocols,
the Waters 845, Chromatography
software
Figure3: Enhanced
GraphicDisplayof System
Suitability
Results
..........................................................................
Syste,,,
Suitability
Results
fo, Hydrochlorothiazide
andTimolol
Maleale
'1.8
1.7
1.6
AREART
= Retention=
Areo Time
'1 . 5
HOT = Height
_1. 4
TR =
Factor
: Tailing
Resolution
N =. Plate Count
1 . 3
I. 2
% _.
1.0
R 0.9
S 0.8
D 0.7
mance. The software calculates plate
count, resolution, and tailing factors,
determines percent relative standard
0.5
0.4
0.3
deviation
chromatographic
for retention
time,
parlorarea,
height, plate
count,system
resolution
and
tailing factors for single vials or entire
multi-methods.
0.1
0.6
O.P
0.0
Waters Custom Convert manager
transfers summary reports into third
part,/software formats (RS-1,Lotus
1-2-3, 20/20 and ASCII) for further
statistical analysis and graphic presentations as shown in Figure 3.
communication
provides
unlimitedmethodsdevelopment
optionsandsophisticated
multipledetectorcontrol.
LEGEND
[-_
HYDROCH
_
TINOLOL
_
,
RT
AREA
,I1
HGT
r- 1
_,
1:1
N
...............................................................................
Analyticalresultscanbe converted
to a thirdpartysoftwareformatforenhancedvisualdisplays.In
thisexample,system
suitabililyresultsfromtheanalysisof a calibrationstandardcontaininghydrochlorothiazide
andtimololmaleatewereconvertedtoa formatcompatiblewithAccessTechnology's
20/20software,andthengraphedtodemonstrate
advancedreportingcapabilities
of the845system.
-6-
Waters Extends its Family of Sample Processors
_;at__'rs
715 and 1700 WISP
SampleProccs._rs for Advanced
SampleHandling
exclusive fluid path design that ensures
accuracy and reproducibility for all
injector volumes while eliminating
Waters has introduced two new products to extend its line of auto injectors,
the 715 Ultra WISP and the 1700 WISP
cross-contamination and carry-over•
A choice of sample injection volumes, from 1 microliter to 2000 micro-
Sample Processors. The 715 offers full,
flexible programmability with easy-
liters and carousels with either 48
four-milliliter or 96 one-milliliter vials,
to-use menus and CRTdisplay and an
all-electric valve design. It is ideal for
applications involving reagent additions and derivatization. It is compatible with automated workstations such
are available.
The Waters 1700 WISP Sample
Processor provides automated sample
preparation with Waters Millilab Workstation, plus direct sample injection for
as the Millilab'" 1A Sample Preparation Workstation.
on-line HPLC analysis. This innovative
system performs filtration, solid phase
The WISP 715 is compatible with
Waters PowerLine HPLC system for
single keyboard control and documentation of al) operating system
parameters. In addition, it features an
extraction, liquid-liquid extraction,
mixing, addition of internal standards,
heating, cooling, evaporation, trace
enrichment, plus many other repetitive
sample cleanup routines completed
unattended,
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Notes Free Column Offer
We would like to receive editorial contributions from you. If you have an
HPLC, IC or GPC application or an
improved sample preparation technique that you would like to share
with our readership, we would like
to include it in a future issue of
Pharmaceutical Notes .....
In exchange, we will send you a
free analytical reverse-phase C]8 column of your choice in a Nova-Pak TM,
i_Bondapak'" or Resolve" chemistry
if your contribution isaccepted.
-7-
-'
Pleasesend your suggestions to:
Carole Wade-Clark MC
Waters Division of Millipore
34 Maple Street, Milford, MA 01757
How to receive future"
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issues of Pharmaceutical
Notes
alesin thisissue,contact your nearest
Waters technical representative or call
508-478-2000.
Waters Chromatography Division
Millipore Corporation
34 Maple Street
Milford,
MA
508-478-2000
01757
NJ
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Telex 698 371 F
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and MilliLab are trademarks of Millipore Corporation.
Lotus 1-2-3 is a registered trademark of Lotus Corporation.
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Pharmaceutical
Notes is published
by the Pharmaceutical
Group of Waters.
Waters
D,v,s,on
of ,NailI IPOR[
@,1989 Milhpore Corporation
Primed in the U.S.A.
8/89
WN040
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