Dendrosome-based delivery of siRNA against Hsp27 in human

Tabriz University of Medical Sciences ID: 6939 Congress: 1st Tabriz International Life Science Conference and 12th Iran Biophysical Chemistry Conference Title: Dendrosome-based delivery of siRNA against Hsp27 in human hepatoma cells Authors: Marziyhe Karimi ¹,Reyhaneh Ramazani¹, Majid Sadeghizadeh ¹* Abstract: <meta content="text/html; charset=utf-8" http-equiv="Content-Type" /> <meta content="Word.Document" name="ProgId" /> <meta content="Microsoft Word 12" name="Generator" /> <meta content="Microsoft Word 12" name="Originator" /> <link href="file:///C:\Users\a\AppData\Local\Temp\msohtmlclip1\01\clip_filelist.xml" rel="File-List" /> <link href="file:///C:\Users\a\AppData\Local\Temp\msohtmlclip1\01\clip_themedata.thmx" rel="themeData" /> <link href="file:///C:\Users\a\AppData\Local\Temp\msohtmlclip1\01\clip_colorschememapping.xml" rel="colorSchemeMapping" /> <style> <!-/* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 415 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-520092929 1073786111 9 0 415 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0in; margin-right:0in; margin-bottom:10.0pt; margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} .MsoPapDefault {mso-style-type:export-only; margin-bottom:10.0pt; line-height:115%;} @page Section1 {size:8.5in 11.0in; margin:1.0in 1.0in 1.0in 1.0in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> </style>  Introduction: Lipofectamine 2000 has been used successfully to transfect short interfering RNAs (siRNA) into mammalian cells for RNA interference (RNAi) studies but  Experiments showed that liofectamin has toxic effects on target cells which necessitates using a safer career such as dendrosome. dendrosomes are neutral, biodegradable, covalent or selfassembled,hyperbranched, spheroidal nano-particles with size ranging from 15 to 100nm that provides an  efficient mean for  gene delivery into various kinds of cells such as human hepatoma. In this experiment, We've used dendrosome As a safe carrier to transfect short interfering RNAs (siRNA) into human hepatoma cell. Methods: study was conducted on Huh7 cells. Under sterile conditions 4mL of the culture  medium (DMEM) and 10% FBS  was pipetted into a 25cmᵌ flask (37 ◦C). After 72 hours , Cells were cultured in  a  plates 12 wells. and After 24  hours , Dendrosome –si RNA and lipofectamin –siRNA  Complexes  were  prepared and  added to each well.At time intervals of 24 and 48 and 72 hours,  RNA  was extracted  and examined by  RT -PCR . Results: The results indicate that siRNA  is transferred into huh-7 cell and Expression levels of hsp 27 are decreased. Conclusions: dendrosomes is neutral, biodegradable, covalent or selfassembled,hyperbranched, spheroidal nano-particles that  can be a good alternative to other carriers such as Lipofectamin in the gene delivery. KEYWORDS: Lipofectamine 2000, short interfering RNAs, dendrosomes, RT –PCR Presentation: Poster

Dendrosome-based delivery of siRNA against Hsp27 in human

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