The Biotechnology Education Company®
®
S-74
EDVO-Kit #
What Is Osmosis?
Storage:
Store this experiment a room temperature.
EXPERIMENT OBJECTIVES:
•
•
•
Students will predict the net movement of
molecules across a semipermeable membrane.
Students will define what molecules move during
diffusion and osmosis.
Students will understand that osmosis and
diffusion are important principles in animal and
plant physiological systems.
All components are intended for educational research only.
They are not to be used for diagnostic or drug purposes, nor
administered to or consumed by humans or animals.
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2
EDVO-Kit # S-74
What is Osmosis?
Table of Contents
Page
Experiment Components
Experiment Requirements
3
3
Background Information
4
Experiment Procedures
Experiment Overview
Activity One - Learning About Osmosis and
6
Dialysis Membrane
Activity Two - Effect of Salt on Osmosis
7
8
Activity Three - Dialysis of Juice Pigments
Critical Thinking and Hypothesis Development
9
10
Study Questions
10
Instructor's Guidelines
Notes to the Instructor
Suggestions for Lesson Plan Content
11
12
Connections to National Content Standards
Connections to National Skill Standards
13
14
Pre-Lab Preparations
Study Questions and Answers
14
15
Material Safety Data Sheets
16
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EDVO-Kit # S-74
What is Osmosis?
3
Experiment Components
This experiment is designed for 5 groups.
Contents
•
•
•
•
Storage:
Store entire experiment
at room temperature.
Orange Dye, Low Molecular Weight Dye
Blue/Green Dye, High Molecular Weight Dye
1 Roll of Dialysis Tubing (7 feet)
5 Transfer pipets
Requirements
•
•
•
•
•
Table Salt
Beakers or Jars (600 ml, approximately the size of the packaging tube)
Distilled or Deionized Water
Spoon
Apple Juice, Beet Juice, or Equivalent Juices
All components are
intended for
educational research
only. They are not to
be used for
diagnostic or drug
purposes, nor
administered to or
consumed by
humans or animals.
EDVOTEK - The Biotechnology Education Company ®
1-800-EDVOTEK • www.edvotek.com
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4
Sci-On® Biology
EDVO-Kit # S-74
What is Osmosis?
Background Information
An Introduction to Osmosis
Molecules undergo constant motion, which enables them to move
from one region to another. The motion of a molecule in solution is
constantly affected by collisions with other molecules, which results in
random motion. Diffusion, which is important to living systems, occurs
in response to molecular motion and to a concentration gradient,
that is, molecules moving from areas of high concentration to areas
of low concentration until an equilibrium is reached. Examples
include the oxygenation of blood in the lungs and the exchange of
nutrients into cells and waste products out of cells.
During osmosis, the diffusion of water molecules down a concentration gradient occurs across a semi-permeable membrane. A semi–
permeable membrane is a membrane which allows some materials to
pass through while rejecting others. This is true of the plasma membrane surrounding all living cells. Dialysis tubing can be used to
demonstrate the properties of a semi-permeable membrane in
relation to osmosis. For example, if one fills a piece of dialysis tubing
with a 5% salt solution (95% water) and places the tubing into a 100%
water bath, water molecules will move from the water bath, a region
with a higher concentration of water
molecules, into the tubing which has
a lower concentraion of water
molecules. The tubing, being semipermeable, allows only the smaller
water molecules to move through the
pores. The membrane is impermeable to salt and therefore salt cannot
move through the pores. The net
result is a swelling in the bag size due
to the influx of water molecules.
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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Sci-On® Biology
EDVO-Kit # S-74
What is Osmosis?
An Introduction to Osmosis
Dialysis membranes are made of purified cellulose containing microscopic pores. The pore size is controlled during manufacturing. The
pore size determines the membrane’s permeability to molecules of
different sizes. This assumes that these molecules have similar shapes.
The dialysis tubing being used in this experiment will allow only low
molecule weight molecules to pass through the membrane, while
larger molecules will remain in the dialysis tubing.
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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Background Information
If the liquid in the dialysis tubing is 100% water and the bath outside
the tubing is a 5% salt solution, then there will be a flux of water out of
the tubing, which will result in shrinkage of the tubing. In the case
where the concentration of water is equal on both sides of the dialysis
membrane, that is, the liquid in the bath is the same concentration as
that in the dialysis tubing, then no change in tubing will result. An
equilibrium exists between the water molecules inside and outside the
tubing. (Figure 3)
6
EDVO-Kit # S-74
What is Osmosis?
Sci-On® Biology
Experiment Overview
EXPERIMENT CONTENT OBJECTIVE
Experiment Procedures
•
•
•
Students will predict the net movement of molecules across a
semipermeable membrane.
Students will define what molecules move during diffusion and
osmosis.
Students will understand that osmosis and diffusion are important
principles in animal and plant physiological systems.
WORKING HYPOTHESIS
If the movement of molecules is from a higher concentration to a
lower concentration through a semi-permeable membrane, then
qualitative results to demonstrate this principle can be observed in
experimental procedures.
BEFORE YOU START THE EXPERIMENT
1.
Read all instructions before starting the experiment.
2.
Write a hypothesis that reflects the experiment and predict
experimental outcomes.
LABORATORY SAFETY
Wear gloves
and safety
goggles
1.
Gloves and goggles should be worn routinely as good
laboratory practice.
2.
DO NOT MOUTH PIPET REAGENTS - USE PIPET PUMPS.
3.
Always wash hands thoroughly with soap and water
after handling reagents or biological materials in the
laboratory.
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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7
Sci-On® Biology
EDVO-Kit # S-74
What is Osmosis?
Activity One - Learning about Osmosis and Dialysis Membrane
WORKING HYPOTHESIS
EXPERIMENT PROCEDURE
1.
Prepare two pieces of dialysis tubing as follows:
Carefully tie an overhand knot at one end of moist dialysis tubing.
Tie the knot approximately one inch from the end.
2.
Prepare the dialysis bath by completely filling a clean beaker or
jar with water.
3.
Fill the first dialysis bag with blue/green dye:
•
•
•
•
•
4.
Roll the untied end of the dialysis bag between your thumb
and pointer finger to open the end of the bag.
Squeeze the transfer pipet bulb and fill it with dye up to a
level just below the pipet bulb.
Gently insert tip of the transfer pipet deep into the bag.
Apply pressure on the bulb to transfer the contents of the
pipet to the dialysis bag.
Carefully tie a knot at the open end of the bag and place it
in the beaker of water.
Rinse the transfer pipet in water and fill the second dialysis bag
with orange dye. Follow the same procedures as in step 3.
You should now have two filled dialysis bags (one blue/green,
and one orange) in a beaker of water.
Save the dialysis tubes
that still contain dye in a
container of water until
you are ready to
perform Activity Two.
5.
After 15 minutes, observe if there is any color change in the
dialysis bath water.
6.
One at a time, remove each bag filled with dye and observe its
contents. Is there any change in the color? Put the dialysis bags
back in the beaker.
7.
Periodically check the color of the bags and dialysis bath water
and determine if any color change has occurred in either the
bags or the bath water.
8.
Which of the two dyes has a smaller molecular size? Why? Which
has a larger molecular size.
9.
Record your results.
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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Experiment Procedures
If a semi-permeable membrane allows for molecules of a certain size
to pass through, then one can determine relative sizes of colored
molecules based on color changes in the surrounding solution.
8
EDVO-Kit # S-74
What is Osmosis?
Sci-On® Biology
Activity Two - Effect of Salt on Osmosis
Experiment Procedures
WORKING HYPOTHESIS
If water molecules move from a higher concentration of water
molecules to a lower concentration, then a dialysis bag containing a
colored dye and placed into a salt solution, will shrink in size if the
concentration of water molecules is higher in the bag than in the
surrounding solution.
EXPERIMENT PROCEDURE
1.
Prepare a fresh dialysis bath as described in Activity One.
2.
Add 3 Tablespoons of salt to the dialysis bath. Stir until the salt is
dissolved.
3.
From Activity One, obtain the dialysis bag that still contains dye.
Hint: This is the high molecular weight dye, and it did not pass
through the dialysis membrane.
4.
Place the dialysis bag saved from Activity One into the dialysis
bath that now contains salt.
5.
After 15 minutes, observe if there is any change in the dialysis
bag. Has the color changed? Does the volume of the bag
appear the same or different?
6.
Periodically check to see if any changes in color or volume has
occurred.
7.
Initially, there will be a flux of water out of the dialysis bag into the
bath, which has a high salt concentration. This will result in
shrinkage of the volume inside the bag. What happens to the
dye when the volume shrinks?
8.
Record your Results
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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Sci-On® Biology
EDVO-Kit # S-74
What is Osmosis?
Activity Three - Dialysis of Juice Pigments
WORKING HYPOTHESIS
EXPERIMENT PROCEDURE
1.
Obtain a clear, non-particulate fruit or vegetable juice that
contains a pigment, such as beet, cranberry, or apple juice.
Each group can use the same juice, or can choose different
juices.
2.
Prepare a fresh dialysis bag and a fresh dialysis bath as in Activity
One.
3.
Prepare the dialysis bag with the juice your group has chosen
and place it in the dialysis bath.
4.
After 15 minutes, observe if there is any color change in the
dialysis bath water or dialysis bag.
5.
Periodically check the color of the bags and dialysis bath water
and determine if any color change has occurred in either the
bags or the bath water.
6.
From the dialysis, it is possible to visually determine the relative size
of some dye molecules. In this activity, the semi-permeable
membrane will permit the passage of molecules which are small,
but will not allow the passage of large ones.
7.
What can you tell about the size of the juice pigment chosen by
your group? Compare your group's result with the results from the
other four groups in your class.
8.
Record your Results
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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Experiment Procedures
If a semi-permeable membrane allows for molecules of a certain size
to pass through, then one can determine relative sizes of juice
pigments based on color changes in the surrounding solution.
10
EDVO-Kit # S-74
What is Osmosis?
Sci-On® Biology
Critical Thinking and Hypothesis Development
Experiment Procedures
LAB
NOTEBOOK
Record the following in your Laboratory Notebook or as instructed by
your teacher. For each activity (Activities 1, 2 and 3), answer the
following:
1.
What is the variable?
2.
What is the control?
3.
What could be changed if the activity was repeated?
4.
Write a hypothesis that would reflect a change.
Study Questions
LAB
NOTEBOOK
Record the answers to the following Study Questions in your Laboratory Notebook or as instructed by your teacher.
1.
Differentiate between diffusion and osmosis.
2.
What evidence from this experiment supports the idea that
molecules are constantly in motion?
3.
What is meant by a semi-permeable membrane?
4.
What determines a membrane’s permeability to molecules of
different sizes? Give a concrete example from Activity #1.
5.
In Activity #2, what happened to the overall size of a dialysis bag
when placed into a salt dialysis bath? Explain.
6.
Give examples from Activity #3 of different juice pigments that
pass through and those that do not pass through a semi-permeable membrane.
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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11
EDVO-Kit # S-74
What is Osmosis?
Sci-On® Biology
Notes to the Instructor
Class size, length of laboratory sessions, and availability of equipment
are factors which must be considered in the planning and the implementation of this experiment with your students. These guidelines
include Suggestions for Lesson Plan Content which can be adapted
to fit your specific set of circumstances.
EDVOTEK experiments are easy to perform and are designed for
maximum success in the classroom setting. However, even the most
experienced students and teachers occasionally encounter experimental problems or difficulties.
The EDVOTEK web site provides a variety of resources which are
continuously being updated and added. If you do not find the
answers to your questions in this section or at the EDVOTEK
web site, Technical Service is available from 9:00 am to 6:00
pm, Eastern time zone. Call for help from our knowledgeOrdering
able technical staff at 1-800-EDVOTEK (1-800-338-6835).
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Please have the following information:
• The experiment number and title
• Kit Lot number on box or tube
• The literature version number
(in lower right corner)
• Approximate purchase date
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
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Instructor’s Guide
EXPERIMENT HINTS AND HELP
12
EDVO-Kit # S-74
What is Osmosis?
Sci-On® Biology
Suggestions for Lesson Plan Content
This lesson plan outline, written by a teacher, can be used as a
guideline for classroom discussion. Connections to the National
Content and Skills Standards follow.
Instructor’s Guide
Purpose
To introduce students to the principle behind the movement of
molecules across cell membranes.
Focusing Event
Students should understand the concept behind the net movements
of molecules. They should be able to distinguish between diffusion
and osmosis. They should understand what semi-permeable means in
relation to the movement of molecules.
Prerequisite Knowledge
Information covered in the background and introduction section
found on page should be thoroughly discussed both before setting
up the experiment and after the experiment has been run.
Experimental Skills
Students will set up three experimental chambers with each one
holding a dialysis bag filled with an experimental solution. They will
compare and observe either color or volume changes within the
dialysis bogs as they investigate the meaning of diffusion and osmosis.
Materials
Components which are provided with this kit, and a list of additional
requirements to perform this experiment are listed on page 3.
Extensions
Quantify Results. In all three activities, students should weigh the
dialysis bags before and after emersion into the solution baths.
Guidelines for Classroom Discussion
1.
Have students become familiar with these vocabulary words:
Diffusion
dialysis
semi-permeable
permeable
Osmosis
concentration gradient
2.
Discuss their observations in class.
3.
Discuss study questions as a class.
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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Sci-On® Biology
EDVO-Kit # S-74
What is Osmosis?
Connections to National Content Standards
1.
Students will develop abilities necessary to do scientific inquiry.
•
2.
Students will develop an understanding through inquiry. Students
will:
•
investigate a working hypothesis.
•
make careful observations.
•
interpret results correctly.
•
understand the difference between the experiment and the
control.
•
identify the variable.
•
predict experimental outcomes.
•
formulate explanations from evidence.
•
develop alternative explanations.
Students will use materials and techniques for experimentation
and direct investigation of phenomena.
•
4.
Students will understand and interpret principles involved in
the movement of molecules in relation to a concentration
gradient.
Students will develop an understanding of the principles of
diffusion and osmosis. Students will:
•
understand that molecules are constantly in motion and
move down the concentration gradient, or from high concentration to low.
•
observe that is osmosis, the diffusion of water molecules
across a semi-permeable membrane, is from low salt (or high
water) to high salt (or low water) concentrations.
•
understand that pore size determine a membranes permeability to molecules of different sizes.
•
understand that the principles of osmosis and diffusion of
molecules across semi-permeable membranes are involved
in the physiological processes of animal systems.
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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Instructor’s Guide
3.
Students’ questions will be answered through conduction of a
scientific investigation.
14
EDVO-Kit # S-74
What is Osmosis?
Sci-On® Biology
Connections to National Skill Standards
In this experiment students will use procedures that allow for the
investigation of the movement of molecules across a semi-permeable
membrane.
Instructor’s Guide
Students will be able to:
1.
Perform all experimental steps accurately.
2.
Make careful observations and record and interpret results.
3.
Predict experimental outcomes.
4.
Share and compare results with other students.
5.
Make both qualitative observations and quantitative measurements.
1.
Cut the dialysis tubing into smaller pieces - each piece should be
about 5 and 1/2 inches long. You should get at least 15 pieces
from the tubing provided.
2.
Soak the cut tubing in a clean beaker or jar filled with distilled
water. Soak the tubing for at least one hour or overnight.
Pre-Lab Preparations
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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15
Sci-On® Biology
EDVO-Kit # S-74
What is Osmosis?
Study Questions and Answers
1.
Differentiate between diffusion and osmosis.
Diffusion is the movement of molecules down a concentration
gradient from high to low. Osmosis is the movement of water
molecules across a semi-permeable membrane from high
concentration of water molecules to a lower concentration.
What evidence from this experiment supports the idea that
molecules are constantly in motion?
The sizes of the dialysis bags changed and the color of the dialysis
bath changed to indicate the movement of molecules and
water.
3.
What is meant by a semi-permeable membrane?
A semi-permeable membrane is a membrane which allows
some materials, such as water, to pass through while rejecting
others, such as salt.
4.
What determines a membrane s permeability to molecules of
different sizes? Give a concrete example from Activity #1.
A membranes pore size will determine what size molecules pass
through. The smaller colored dye molecule will cause the dialysis
bath to become colored.
5.
In Activity #2, what happened to the overall size of a dialysis bag
when placed into a salt dialysis bath? Explain.
The bag shrunk in size because the water molecules moved into
the salt dialysis bath.
6.
Give examples from Activity #3 of different juice pigments that
pass through and those that do not pass through a semi-permeable membrane.
Answers will vary based on juices used.
Duplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/
laboratory use only. This document, or any part, may not be reproduced or distributed for any other purpose
without the written consent of EDVOTEK, Inc. Copyright © 1996, 1997, 1998, 1999, 2003, 2007 EDVOTEK, Inc., all
rights reserved.
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Instructor’s Guide
2.
Section V - Reactivity Data
Material Safety Data Sheet
®
IDENTITY (As Used on Label and List)
Incompatibility
Section I
Emergency Telephone Number
Manufacturer's Name
EDVOTEK, Inc.
(301) 251-5990
(301) 251-5990
Date Prepared
14676 Rothgeb Drive
Rockville, MD 20850
Hazardous Decomposition or Byproducts
Burning will produce oxides of carbon, nitrogen and sulfur
Hazardous
Polymerization
Inhalation?
OSHA PEL
ACGIH TLV
Other Limits
Recommended
% (Optional)
Overexposure may cause eye or skin irritation
Overexposure may cause eye or skin irritation.
May cause respiratory irritation.
Emergency First Aid Procedures
Eyes: Flush continuously with flowing water for at least 15 min.
Skin: Wash thoroughly with plenty of water & soap. Respiratory: Remove to fresh air. If distress continues,
call doctor.
Ingestion: If conscious, drink 2 glasses of water to dilute. Call poison control center.
Steps to be Taken in case Material is Released for Spilled
Specific Gravity (H 0 = 1)
2
N/A
N/A
N/A
Melting Point
N/A
N/A
Evaporation Rate
(Butyl Acetate = 1)
N/A
Mix material with absorbing compounds. Sweep up and dispose accordingly.
Waste Disposal Method
Dispose of waste in accordance with current Federal, State and Local codes and guidelines.
Precautions to be Taken in Handling and Storing
Maintain good housekeeping to avoid spillage. Avoid breathing mist. Avoid contact with eyes and skin.
Store in a dry place away from excessive heat, in original or similar waterproof container.
Other Precautions
Section IV - Physical/Chemical Characteristics
Extinguishing Media
N.D. = No data
Flammable Limits
N.A.
N.A.
LEL
N.A.
UEL
Water spray, dry chemicals, foam or carbon dioxide
Section VIII - Control Measures
Respiratory Protection (Specify Type)
NIOSH/MSNA approved Dust Respirator
Local Exhaust
Ventilation
Special
Yes
Other
Mechanical (General)
Special Fire Fighting Procedures
Firefighters should wear full-face, self contained breathing apparatus and impervious
protective clothing.
Unusual Fire and Explosion Hazards
OSHA Regulation?
Section VII - Precautions for Safe Handling and Use
Liquid green, slightly sweet odor
Flash Point (Method Used)
IARC Monographs?
NTP?
Medical Conditions Generally Aggravated by Exposure
Completely soluble
Appearance and Odor
Yes
Signature of Preparer (optional)
Section III - Physical/Chemical Characteristics
Solubility in Water
Ingestion?
Yes
Not considered to be hazardous
Liquid Green Dye
No Hazardous ingredients present
Vapor Density (AIR = 1)
Skin?
Yes
Health Hazards (Acute and Chronic)
Carcinogenicity:
Hazardous Components [Specific
Chemical Identity; Common Name(s)]
Vapor Pressure (mm Hg.)
X
Section VI - Health Hazard Data
Signs and Symptoms of Exposure
For 1% solution
Conditions to Avoid
May Occur
Will Not Occur
02/20/2007
Section II - Hazardous Ingredients/Identify Information
Boiling Point
Reducing agents
Strong oxidizing agents
Route(s) of Entry:
Telephone Number for information
Address (Number, Street, City, State, Zip Code)
X
Stable
Note: Blank spaces are not permitted. If any item is not
applicable, or no information is available, the space must
be marked to indicate that.
Blue/Green Dye
Conditions to Avoid
Unstable
Stability
May be used to comply with OSHA's Hazard Communication
Standard. 29 CFR 1910.1200 Standard must be consulted for
specific requirements.
Protective Gloves
Eye Protection
Impervious gloves
Chemical safety goggles
Other Protective Clothing or Equipment
Eye wash fountain and safety shower should be readily available
Work/Hygienic Practices
Wear protective clothing and wash thoroughly after handling.
None expected
Section V - Reactivity Data
Material Safety Data Sheet
EDVOTEK
IDENTITY (As Used on Label and List)
Incompatibility
Section I
Emergency Telephone Number
Manufacturer's Name
EDVOTEK, Inc.
(301) 251-5990
Strong oxidizing agents
Hazardous Decomposition or Byproducts
Toxic fumes of Carbon Monoxide, Carbon Dioxide, Nitrogenoxide, Sulfur Oxides
Hazardous
Polymerization
(301) 251-5990
07/01/03
X
Section VI - Health Hazard Data
Inhalation?
Carcinogenicity:
Ingestion?
Skin?
Yes
Health Hazards (Acute and Chronic)
Yes
Yes
May cause irritation
Date Prepared
14676 Rothgeb Drive
Rockville, MD 20850
Conditions to Avoid
May Occur
Will Not Occur
Route(s) of Entry:
Telephone Number for information
Address (Number, Street, City, State, Zip Code)
X
Stable
Note: Blank spaces are not permitted. If any item is not
applicable, or no information is available, the space must
be marked to indicate that.
Orange Dye
Conditions to Avoid
Unstable
Stability
May be used to comply with OSHA's Hazard Communication
Standard. 29 CFR 1910.1200 Standard must be consulted for
specific requirements.
®
IARC Monographs?
NTP?
No data
Signature of Preparer (optional)
OSHA Regulation?
Signs and Symptoms of Exposure
No data
Section II - Hazardous Ingredients/Identify Information
Hazardous Components [Specific
Chemical Identity; Common Name(s)]
OSHA PEL
ACGIH TLV
Other Limits
Recommended
Medical Conditions Generally Aggravated by Exposure
No data
% (Optional)
CAS # 547-58-0
This product contains no hazardous components
Emergency First Aid Procedures If swallowed, wash mouth with water. Skin contact, flush with water.
If inhaled, remove to fresh air.
Section VII - Precautions for Safe Handling and Use
Section III - Physical/Chemical Characteristics
Boiling Point
Vapor Pressure (mm Hg.)
Vapor Density (AIR = 1)
Solubility in Water
Steps to be Taken in case Material is Released for Spilled
Wear SCBA and protective clothing. Mop with Absorbent material. Dispose of properly.
No data
Specific Gravity (H 0 = 1)
2
No data
No data
Melting Point
N/A
No data
Evaporation Rate
(Butyl Acetate = 1)
No data
Mix w/ combustible solvent and burn in chemical incinerator equipped with afterburner and
scrubber. Observe federal, state, and local laws.
Precautions to be Taken in Handling and Storing
Wear protective equipment
soluble
Other Precautions
Appearance and Odor
Avoid contact and inhalation
Orange Solution
Section IV - Physical/Chemical Characteristics
Flash Point (Method Used)
Extinguishing Media
Waste Disposal Method
Flammable Limits
Section VIII - Control Measures
LEL
UEL
Water spray, CO2, dry chemical powder or appropriate foam
Respiratory Protection (Specify Type)
Ventilation
NIOSH approved respirator
Local Exhaust
Mechanical (General)
Special Fire Fighting Procedures
Protective Gloves
Wear SCBA and protective clothing to prevent contact with skin and eyes
Chemical resistant
Other Protective Clothing or Equipment
Unusual Fire and Explosion Hazards
Emits toxic fumes under fire conditions.
Work/Hygienic Practices
None
Avoid contact and inhalation
Yes
No
Special
Other
Eye Protection
No
None
splash resistant