Volume 8 • Number 1 • 2001
Amino Acid #
Amino Acid #
Amino Acid #
= Semi-Permissive
= Permissive
G20
R388
Y100
R255
Figure 4. The location of the 19-amino acid insertion
into eight independent β-galactosidase insertion
derivatives. The designated amino acid is immediately N-terminal to the point of insertion. The domain
structure of β-galactosidase is adapted from Jacobson
et al., (1994) Nature 369:761.
= Non-permissive
D671
N345
L631
P703
EZ::TN™ In-Frame Linker Insertion Kit
EZI04KN
Domain 1
αComplementation
Region
1
100
Domain 2
200
300
Domain 3
400
500
Domain 4
600
700
TransforMax™ EC100™
Electrocompetent E. coli
Domain 5
800
900
10 Reactions
1000
β-galactosidase
Highest efficiency electrocompetent cells
available. See center insert for more
information.
EC10005
5 X 100 µl
(10 electroporations)
EC10010
10 X 100 µl
(20 electroporations)
Finish your DNA Sequencing Project
Complete Difficult Regions with SequiTherm EXCEL™ II DNA Sequencing Kits
Sequence through hairpin loops, regions
of high GC or AT content, areas of interstrand reannealing, and inverted or
direct repeats. Using end-labeled
primers, SequiTherm EXCEL II DNA
Sequencing Kits provide complete, clear
sequencing data when all other methods
fail.
“I know of no other sequencing
method that resolves GC
compressions better than
SequiTherm EXCEL™ II.”
- Glenn Dawes, Affymax Research Institute,
The Scientist, Vol.12, #6, 1998, pg. 13
SequiTherm EXCEL II resolved a
“2-year-old puzzle” due to “an
unreadable hairpin loop” for the
Washington University genome center.
- Science, Vol. 280, 8 May 1998, pg. 816
G A T C
Benefits
SequiTherm EXCEL™ II DNA
Sequencing Kit - LC For 25-41 cm gels
on LI-COR® Sequencers
• Complete work on most any difficult
gap to finish your sequencing project.
• High flexibility - you can perform
both cycle and isothermal sequencing
with one kit.
• Kits compatible with LI-COR® and
ALF™ DNA Sequencers (both dyeprimer and internal label) and with
ABI™ automated sequencers
(dye-primer).
SE9101LC
Figure. Sequencing through a
GC-rich trinucleotide repeat.
Supercoiled plasmid template
containing (CGG)23 was
sequenced using the
SequiTherm EXCEL II
isothermal sequencing
protocol.
(100 Sequences)
SequiTherm EXCEL™ II DNA
Sequencing Kit - LC For 66 cm gels on
LI-COR® Sequencers
SE9202LC
(100 Sequences)
SequiTherm EXCEL™ II Long-Read™
DNA Sequencing Kit - ALF™
For ALF DNA Sequencers
SE8301A
(100 Sequences)
SequiTherm EXCEL™ II DNA
Sequencing Kit
• Reduced background signal.
(for manual cycle and non-cycle
sequencing)
SEM79020
20 Sequences
SEM79050
50 Sequences
SEM79100
100 Sequences
www.epicentre.com
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