5E Inquiry Lesson Plan Title: Barn Owl Pellet Lab Grade level: 7th National Standards: Interdependence of Life The student will understand that within ecosystems, complex interactions exist between organisms and the physical environment. Animals eat plants or other animals for food and may also use plants (or even other animals) for shelter and nesting. Some source of energy is needed for all organisms to stay alive and grow. Alabama Course of Study: Standards 1 and 7 Objectives: The student will be able to dissect an owl pellet. The student will be able to identify the animal skulls and other bones found in the pellet. The student will view a Power Point Presentation and participate in an interactive class discussion to learn basic facts about the barn owl, food chains and food webs. Procedures Materials: Owl pellets; metric ruler; forceps; wooden probes; hand lens; glue stick; 8 cards labeled with the following bone groups: skull, jaw, scapula, fore limb, hind limb, pelvic, rib, vertebrae; balance scales; paper plates; zip lock bags; pH paper; distilled water; clear plastic containers; 4 bone charts and 6 hand-outs. ENGAGE: 1st Day: Pre-Test; 3-D Presentation Time: 15 minutes EXPLORE: 1st Day: Complete Handout #1. Weigh and measure both owl pellets. Record your findings on weight/measurement on Handout #2. Answer questions 1 and 2 on Handout #2. Time: 30 minutes 2nd Day: Record on Handout #2 the predicted number of skulls for each pellet you weighed and measured on Day 1. Answer question 3 on Handout #2. Dissect the owl pellets and record the observed number of skulls on Handout #2 for your individual pellets. Does your prediction correlate with your observation after dissection? Save and store bones in zip lock bags for the next day’s activity. Use pieces of the fur/hair of each dissected owl pellet to determine the pH of the pellet. Record your results and answer questions 1, 2, 3 and 4 on Handout #3. Time: 45 minutes 3rd Day: EXPLAIN: After all groups have completed their dissections, exchange results with the other groups and record findings on Handout #4 and answer questions 1, 2, 3. Use the data from your combined class data (Handout #4) to complete Handout #5. Using the data from Handout #5 construct a graph (Handout #6) showing the frequency distribution. Time: 30 minutes 3rd Day con’t.: ELABORATE: Show Power Point Presentation along with interactive class discussion. Time: 15 minutes 4th Day: ELABORATE con’t.: Each group will arrange all the bones from both pellets on the 8 bone cards. Each group will construct a Food Chain with a one producer and at least 4 consumers based on your dissection (use the back of Handout #6 for your Food Chain). Time: 35 minutes EVALUATE: Post Test Time: 10 minutes Safety: N/A Prepared by: Dr. Debbie Payne, Ruth Liddell, Shirley Scarbrough Alabama State University, Math and Science Partnership (MSP) 2012 OWL PELLET INTRODUCTION and INSTRUCTIONS Owls are predators. Most larger owls feed on small mammals, birds and reptiles. Owls usually swallow their food whole or in large chunks. The owl’s digestive system cannot digest fur/hair, bones, teeth, feathers, etc. Rather, a portion of the owl’s stomach compresses these parts to form the pellet. The pellet does not pass into the intestine of the owl; instead, the owl regurgitates (coughs up and spits out) the pellet. In an owl pellet, in addition to bones, you may find the exoskeletons of insects, feathers, fur/hair, fish scales, small white grubs and various types of seeds. By examining what is found in the pellet, you, as well as scientists, can tell what and how much an owl has eaten. In addition, when the acidity or alkalinity of the pellet is measured, predictions can be made about the habitat and/or environment and the possible impact on the owl’s food sources. 1. READ the INTRODUCTION 2. Check your Owl Pellet Study Kit to make sure you have: 2 – Wooden probes 4 – Laminated bone charts showing types of bones commonly found in owl pellets 1 – Hand lens 1 – Pair of forceps 2 – small ziplock bags, labeled Pellet 1 and Pellet 2 1 – Metric ruler Plastic containers 1 and 2 and 2 pieces of pH paper 3. Your teacher will provide: Scales Paper plates, Glue Stick 2 – Owl pellets per group Distilled water 6 – Handouts associated with the Lab that each group will complete with the required data and questions to be turned in 8 – Bone charts to be assembled using bones obtained from the pellet dissections PROCEDURE 1. Before you begin the dissection, complete and turn in Handout #1. 2. Unwrap the aluminum foil from each pellet. Carefully weigh and measure the length and width of each pellet and record data in Handout #2. Read question 1 and answer in the space provided. Observe the outer surface of the pellet and answer question 2. You cannot complete this handout until you have completed the dissection. After completing the dissection, finish the table on Handout #2 and answer question 3. 3. Place each unwrapped pellet on a paper plate labeled Pellet 1 and Pellet 2. Using your fingers or a probe, very gently break each pellet in half. You must be very careful when teasing out the bones from each pellet; the bones are easily broken. 4. Continue to separate small sections of each pellet and remove fur/hair to disclose the bones. Remember: the bones are small and delicate, so you must be very slow and careful in extracting any bones from the pellet. Also, if your pellet has a skull, you will need to pick away all the fur/hair in order to expose the entire skull. You can then try to identify which type of animal the skull came from using your Owl Pellet Bone Chart which shows skulls from four different animals. 5. Gently rub small pieces of each pellet between your fingers until you have completely taken apart and examined the entire pellet and removed all the bones. There are many tiny ribs and vertebrae that should not be overlooked. You have to be very focused so that you do not miss any bones. Set aside any other materials you find in each pellet that are not bones or fur/hair. You will record these on Question 3 on Handout #2. 6. Once you are certain that no bones remain in the fur/hair, place the bones from Pellet 1 into the ziplock labeled Pellet 1 and the bones from Pellet 2 into the ziplock labeled Pellet 2. These will be saved for an activity later in which you will arrange various types of bones on Bone Charts. 7. After you have completed the dissection, record the number of observed skulls from each pellet on Handout #2. 8. Using your fingers, pulverize (rub your fingers together) a small amount of the fur/hair from Pellet 1 and place into the plastic container 1 and a small amount from Pellet 2 and place into plastic container 2. Pour the distilled water over each pulverized pellet; stir with a tooth pick until most of the material has dissolved. Place a piece of the pH paper in each mixture to moisten; remove the paper and compare the color to the pH color scale provided to determine the pH value of each owl pellet mixture. Record your results on Handout #3 and answer questions 1, 2, 3 and 4. 9. Compare your findings from Handout #2 with all other groups findings and record their data in your Handout #4 and answer questions 1, 2, and 3 at the bottom of the Handout. 10. Look at the data in your Handout #4 (combined class data) to fill in the required information on Handout #5. 11. Use the data on Handout #5 to construct a graph (Handout #6) showing the Frequency Distribution**. On the graph, you are developing a frequency distribution which shows the number of times dissected owl pellets contain different numbers of skulls. **A frequency distribution is a statistical concept which uses data arranged to show the number of times an event occurs in a particular way. 12. When you complete your graph, construct (on the back of Handout #6) a Food Chain with at least 4 producers and/or consumers. 13. Glue the bones you saved from your two pellets onto the eight Bone Cards, arranging the bones according to bone type. Label each card with the Study Kit number (your group number). BARN OWL PELLET LAB WHAT DOES A BARN OWL LOOK LIKE? White heart shaped face Whitish belly with dark spots Upper body golden with dark flecks Dark brown eyes Body Length: 15 to 21 inches; long legs Wing span: 43 to 47 inches HABITAT and SENSES OF THE BARN OWL The Barn Owl is found on every continent except Antarctica. They are found in farm lands, grass lands marshes, deserts and suburbs. Owls are nocturnal and have many adaptations that enable them to hunt at night. A two pound owl has eyes the same size as the human eye. Both the eyes and the ears are located at the front of the head which enables excellent depth perception. The feathers around their eyes and ears are shaped to funnel light and sound to their eyes and ears more precisely. Their ears are at different heights which enable them to locate their prey with extreme accuracy. Their sense of hearing is so acute they can hear a mouse’s footsteps 30 yards away flying 10 feet over the field in total darkness. ear opening Owls along with hawks, eagles, osprey and other birds of prey are known as Raptors. RAPTORS Hawks, eagles, osprey and owls are types of raptors. Raptors have hooked beaks and talons (sharp claws) adapted for seizing prey. NOTE! PELLETS ARE NOT OWL POOP! Golden Eagle Red Tail Hawk Red Tail Hawk Barred Owl Osprey Hawks, eagles and osprey tear and swallow small pieces of flesh and avoid swallowing most fur and bones. However, owls swallow prey whole or in large pieces and have a specialized digestive system in which pellets are formed and regurgitated to dispose of the indigestible parts of their prey. DIGESTION: OWL PELLETS A crop is a loose pouch inside the throat of most birds that stores food and allows birds to pulverize hair, bones and teeth so that they can be eliminated from the body. Owls have no crop. The owl’s stomach has two parts . The anterior part is called the proventriculus which produces enzymes, acids and mucus. The second part is called the ventriculus or gizzard which compresses the indigestible part of prey (hair, bones, teeth, feathers, etc.) The stomach muscles form the undigested parts into a wet, slimy pellet. The pellet passes into the proventriculus and remains there for up to ten hours before being regurgitated. When an owl eats more than one prey within several hours, the remains are consolidated into one pellet. New prey cannot be swallowed until the pellet is ejected. Barn owls produce one to two pellets per 24 hours. Much can be learned about an owl’s diet and environment by examining its pellets. Studies of dissected owl pellets provide information about changes in feeding habits that occur from season to season as well as the different species of animals and/or plants that are found in the owl’s habitat. This information allows us to see what role an owl plays in the ecosystem and how they are adapted to their niche. HOW THE ENVIRONMENT IMPACTS WHAT AN OWL EATS A food chain shows the relationship between producers (plants) and consumers (animals that eat the plants or that eat other animals). Food chains only go in one direction. A food chain starts with what gets eaten and has arrows pointing towards what does the eating. Food Webs show how plants and animals are interconnected by different paths. In the wild, animals eat more than one thing and can belong to several food chains. A food web is an elaborate version of a food chain. EFFECTS of ENVIRONMENTAL POLLUTION on pH Pollution can change a water's pH, which in turn can harm animals and plants living in the water. Not only does the pH of a stream affect organisms living in the water, a changing pH in a stream can be an indicator of increasing pollution or some other environmental factor. Excessively high and low pHs can be detrimental for the use of water and can alter the food sources available. One quick way of determining the changing pH of water is to use pH indicator paper as seen in the picture to the right. POWER of the FREE HYDROGEN and HYDROXIDE IONS pH is a measure of how acidic/basic water is. The range goes from 0 - 14, with 7 being neutral. pHs of less than 7 indicate acidity, whereas a pH of greater than 7 indicates a base. pH is really a measure of the relative amount of free hydrogen and hydroxide ions in the water. Water that has more free hydrogen ions is acidic, whereas water that has more free hydroxide ions is basic. Since pH can be affected by chemicals in the water, pH is an important indicator of water that is changing chemically. In ecosystems, plants and animals are all connected. If any one part is removed, it can affect the whole ecosystem. PRE/POST TEST: BARN OWL LAB Circle the correct answer for each of the following questions: 1. The major senses an owl uses in locating prey are: A. Vision and Hearing; B. Hearing and Smell; C. Smell and Taste; D. Vision and Touch. 2. Barn Owls are found on every continent except: A. Asia; B. Artic; C. Antarctica; D. Africa 3. All raptors have all of the following except: A. Teeth; B. Hooked Beaks; C. Sharp Claws; D. Crop 4. The owl’s stomach has: A. One section; B. Two sections C. Three sections; D. Four sections 5. The part of the owl’s stomach that produces enzymes, acids and mucus is: A. Crop; B. Ventriculus; C. Gizzard; D. Proventriculus 6. In a 24 hour period a typical Barn Owl will produce how many pellets? A. Zero; 7. B. One to two; C. Three to four; D. Five or more pH is a measure of the relative amount of _________________ in water: A. free oxygen atoms; B. free hydrogen ions; C. free hydroxide ions; D. both B and C 8. If one part of the ecosystem is removed: A. it affects the entire ecosystem; B. the affect would only involve a small portion of the ecosystem; C. the ecosystem would not be affected; D. only human life would be affected. 9. All of the following is true about a food chain except: A. it shows the relationship between producers and consumers; B. it shows the relationship between plants and animals that eat the plants or that eat other animals; C. it only goes in one direction; D. it is a more elaborate version of a food web. 10. Which of the following show how plants and animals are connected by different paths? A. an ecosystem; B. a food web; C. a food chain; D. none of the above PRE/POST TEST: BARN OWL LAB Circle the correct answer for each of the following questions: 1. The major senses an owl uses in locating prey are: A. Vision and Hearing; B. Hearing and Smell; C. Smell and Taste; D. Vision and Touch. 2. Barn Owls are found on every continent except: A. Asia; B. Artic; C. Antarctica; D. Africa 3. All raptors have all of the following except: A. Teeth; B. Hooked Beaks; C. Sharp Claws; D. Crop 4. The owl’s stomach has: A. One section; B. Two sections C. Three sections; D. Four sections 5. The part of the owl’s stomach that produces enzymes, acids and mucus is: A. Crop; B. Ventriculus; C. Gizzard; D. Proventriculus 6. In a 24 hour period a typical Barn Owl will produce how many pellets? A. Zero; 7. B. One to two; C. Three to four; D. Five or more pH is a measure of the relative amount of _________________ in water: A. free oxygen atoms; B. free hydrogen ions; C. free hydroxide ions; D. both B and C 8. If one part of the ecosystem is removed: A. it affects the entire ecosystem; B. the affect would only involve a small portion of the ecosystem; C. the ecosystem would not be affected; D. only human life would be affected. 9. All of the following is true about a food chain except: A. it shows the relationship between producers and consumers; B. it shows the relationship between plants and animals that eat the plants or that eat other animals; C. it only goes in one direction; D. it is a more elaborate version of a food web. 10. Which of the following show how plants and animals are connected by different paths? A. an ecosystem; B. a food web; C. a food chain; D. none of the above Virtual owl pellet dissection http://www.kidwings.com/owlpellets/flash/v4/index.htm FEMUR, TIBIA/FIBULA Upper – Lower Hind Leg Bones FEMUR, TIBIA/FIBULA Upper – Lower Hind Leg Bones HUMERUS, RADIUS Upper – Lower Front Leg Bones HUMERUS, RADIUS Upper – Lower Front Leg Bones JAWS (mandibles) JAWS (mandibles) PELVIC BONES PELVIC BONES RIBS RIBS SCAPULA (Front Leg Shoulder Blade) SCAPULA (Front Leg Shoulder Blade) SKULLS SKULLS VERTEBRAE VERTEBRAE OWL PELLET HANDOUT #1 What does a common barn owl eat and how much? HYPOTHESIS What do you think you will find in the owl pellet? PREDICTION OWL PELLET HANDOUT # 2 WEIGHT, LENGTH, WIDTH and NUMBER of SKULLS CHART WEIGHT, GRAMS LENGTH, WIDTH, NUMBER of NUMBER of MILLIMETERS MILLIMETERS PREDICTED OBSERVED SKULLS SKULLS PELLET 1 PELLET 2 1. Based on the relative weighs of each pellet, predict the number of skulls in your pellets and record that number above. The range is usually zero to five. 2. Describe at least three physical characteristics of the pellet: A. Smell: B. Color: C. Texture: 3. List any other materials found in your pellets other than fur/hair and bones. Examples: feathers, seeds, insect skeletons, etc. HANDOUT #3 pH ranges from 0 to 14, with 7 being neutral. pHs less than 7 are acidic while pHs greater than 7 are alkaline (basic). Normal rainfall has a pH of about 5.6— slightly acidic due to carbon dioxide gas from the atmosphere. You can see from the chart below, acid rain can be very acidic and it can affect the environment in a negative way. Circle the number on the scale above that corresponds with the pH of Pellet 1. Place a check next to the number on the scale above that corresponds with the pH of Pellet 2. Based on your testing of the pH of Pellet 1 and Pellet 2 answer the following questions: 1. Is Pellet 1 alkaline, acidic or neutral? 2. Is Pellet 2 alkaline, acidic or neutral? 3. The normal pH of an owl pellet is approximately 7.6. Based on your pH measurements of Pellet 1 and Pellet 2, do they indicate that the food source of the owls has been exposed to environmental pollution, such as acid rain? Justify your conclusions. 4. Why is it necessary to use the pH paper to determine the pH of the owl pellet? Explain your answer. OWL PELLET HANDOUT #4 GROUP # 1 2 3 4 5 6 7 8 9 10 11 12 PELLET # 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 WEIGHT, GRAMS PREDICTED # of SKULLS OBSERVED # of SKULLS WRITE Y0UR ANSWERS TO THE QUESTIONS BELOW IN THE SPACE PROVIDED: Question 1: Is there a correlation between the weight of the pellet and the number of skulls? Explain. Question 2: How might you account for the differences in the number of skulls found in each pellet? Question 3: How might you account for the absence of skulls in a pellet? OWL PELLET HANDOUT #5 Using data from the combined class data (HANDOUT #4) fill in the table below. FREQUENCY of SKULLS PER PELLET #SKULLS/PELLETS # PELLETS 0 1 2 3 4 5 6 7 OWL PELLET HANDOUT #6 Use the numbers in HANDOUT #5 to construct a graph showing the frequency distribution of animal skulls in this sample of owl pellets. Frequency Distribution Graph of Animal Skulls per Pellet Data from HANDOUT #5