TCA precipitation protocol 1. 2. 3. 4. 5. Add 10% of TCA (100% w/v) to your sample. Vortex. Incubate 10 min on ice. Spin 10 min at max speed in bench-top centrifuge in cold room. Discard SN, wash pellet with acetone or ethanol, flick-spin. Discard SN, dry pellet in heat block for couple of minutes, resuspend the pellet in 1 X BJ, run the gel.