MICROSCOPE COMPONENTS GUIDE Choosing The Ideal UIS2 Optics Components For Your Equipment 2008-10 The wide range of Olympus components introduced here allows users in such diverse fields as research, inspection and production to take advantage of the quality, flexibility and outstanding optical performance of the UIS2 Optical System. That's why installing Olympus microscope components is, quite simply, the right choice for your equipment. 1 CONTENTS WELCOME TO UIS2/UIS OPTICS ---------------------------------------------- 3 — 4 EYEPIECES/FILAR MICROMETER EYEPIECE --------------------------------- 33 Widefield eyepieces SYSTEM DIAGRAM --------------------------------------------------------------- 5 — 6 Super widefield eyepieces Filar micrometer eyepiece UIS2/UIS OBJECTIVE LENSES ----------------------------------------------- 7 — 18 NEW M Plan Apochromat MPLAPON series -------------------------------8 M Plan SemiApochromat MPLFLN series -------------------------------- 9 Long WD M Plan SemiApochromat LMPLFLN series ---------------------------- 10 M Plan Achromat MPLN series --------------------------------- 11 LCD Long WD M Plan SemiApochromat LCPLFLN-LCD series -------------------------12 Super Long WD M Plan Achromat SLMPLN series -------------------------------13 IR Long WD M Plan Achromat LMPlan-IR series -----------------------------14 IR M Plan Achromat MPlan-IR -------------------------------------14 M Plan SemiApochromat BD MPLFLN-BD series ---------------------------15 M Plan SemiApochromat BDP MPLFLN-BDP series --------------------------16 Long WD M Plan SemiApochromat BD LMPLFLN-BD series --------------------------17 M Plan Achromat BD MPLN-BD series ------------------------------18 WHN10x, WHN10x-H, CROSSWHN10x, WH15x --------------------- 33 SWH10x-H, MICROSWH10x, CROSSSWH10x ------------------------------ 33 U-OSM --------------------------------------- 33 REVOLVING NOSEPIECES ---------------------------------------------------- 34 — 35 BXFM-F -------------------------------------- 19 BXFM-F+BXFM-ILH+BXFM-ILHSPU---------- 20 BXFM-F+BXFM-ILHS ------------------------ 21 SZ2-STU2 ----------------------------------- 22 U-ST ----------------------------------------- 22 SZ-STL -------------------------------------- 22 Quintuple revolving nosepiece U-5RE-2-------------------------------------- 34 Sextuple revolving nosepiece with slider slot for DIC U-D6RE--------------------------------------- 34 Sextuple revolving nosepiece with slider slot for DIC with ESD treatment U-D6RE-ESD --------------------------------- 34 Septuple revolving nosepiece with slider slot for DIC U-D7RE -------------------------------------- 34 Centerable quadruple revolving nosepiece with slider slot for DIC U-P4RE -------------------------------------- 34 Centerable sextuple revolving nosepiece with slider slot for DIC U-P6RE -------------------------------------- 34 Quintuple revolving nosepiece for BF/DF U-5BDRE ------------------------------------ 35 Quintuple revolving nosepiece for BF/DF with slider slot for DIC U-D5BDRE ----------------------------------- 35 Sextuple revolving nosepiece for BF/DF with slider slot for DIC/ U-D6BDRE ----------------------------------- 35 Centerable quintuple revolving nosepiece U-P5BDRE ----------------------------------- 35 Adapter to mount BF objectives BD-M-AD ------------------------------------ 35 ILLUMINATION UNITS --------------------------------------------------------- 23 — 25 VIDEO CAMERA ADAPTERS ------------------------------------------------- 36 — 37 NEW MICROSCOPE SYSTEM BXFM ---------------------------------------------- 19 — 22 BXFM frame BXFM BXFM-S Universal stand type 2 Compact stand Large stand C-mount video camera ports Reflected light illuminator for BF/DF BX-RLA2 ------------------------------------- 23 Universal reflected light illuminator BX-URA2 ------------------------------------ 23 Reflected light illuminators for BF BX-KMA/BX-KMA-ESD ----------------------- 24 Reflected light illuminator for BF U-KMAS ------------------------------------- 25 Video camera mount adapters Video camera port U-TV0.25xC, U-TV0.35xC-2, U-TV0.5xC-3, U-TV0.63xC ------------------- 36 U-CMAD3, U-BMAD, U-SMAD, U-TMAD, U-FMT ----------------------------- 37 U-TV1x-2 ------------------------------------ 37 LAMP HOUSING & ACCESSORIES ----------------------------------------- 26 — 28 MOTORIZED UNITS------------------------------------------------------------- 38 — 41 75W xenon apo lamp housing U-LH75XEAPO ------------------------------- 26 100W mercury apo lamp housing U-LH100HGAPO ----------------------------- 26 100W mercury lamp housing U-LH100HG ---------------------------------- 26 100W halogen lamp housings U-LH100-3/U-LH100IR/U-LH100L-3 -------- 26 External power supply TH4-100/200 -------------------------------- 27 Hand switch TH4-HS -------------------------------------- 27 Extension cord U-RMT --------------------------------------- 27 DF converter for BX-URA2 U-RCV --------------------------------------- 27 Fiber adapter for reflected light observation U-LGAD -------------------------------------- 27 Transmitted light guide adapter SZX-TLGAD ---------------------------------- 27 Light source LG-PS2 -------------------------------------- 28 Light guide LG-SF ---------------------------------------- 28 Double lamp house adapter U-DULHA ------------------------------------ 28 Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple revolving nosepiece+100W halogen lamp housing BX-RLAA+U-D6REMC+U-LH100-3 ---------- 38 Motorized universal reflected light illuminator BX-RFAA ------------------------------------- 38 Motorized sextuple BD revolving nosepiece with slider slot for DIC U-D6BDREMC ------------------------------- 39 Motorized centerable quintuple revolving nosepiece with slider slot for DIC U-P5BDREMC -------------------------------- 39 Motorized quintuple BD revolving nosepiece with slider slot for DIC U-D5BDREMC ------------------------------- 39 Motorized sextuple revolving nosepiece with slider slot for DIC U-D6REMC ---------------------------------- 39 Motorized centerable quintuple revolving nosepiece with slider slot for DIC U-P5REMC ----------------------------------- 39 Control unit BX-UCB -------------------------------------- 39 Hand switch U-HSTR2 ------------------------------------ 39 Control box for motorized nosepiece and BF/DF illuminator BX-REMCB ---------------------------------- 39 AC adapter for BX-REMCB U-ACAD4515 -------------------------------- 39 Active auto focus unit U-AFA2M-VIS -------------------------------- 40 Motorized reflected filter wheel U-FWR --------------------------------------- 40 Motorized illumination with power focus BXFMA-F ------------------------------------ 41 NEW NEW OBSERVATION TUBES -------------------------------------------------------- 29 — 30 Widefield trinocular tube U-TR30-2 ------------------------------------ 29 Widefield trinocular tube for IR U-TR30IR ------------------------------------ 29 Widefield erect image trinocular tube U-ETR-4 ------------------------------------- 29 Single port tube with lens U-TLU ---------------------------------------- 29 Single port tube with lens for IR U-TLUIR ------------------------------------- 29 Super widefield trinocular tube U-SWTR-3 ----------------------------------- 30 Super widefield erect image trinocular tube U-SWETR ------------------------------------ 30 Super widefield erect image tilting trinocular tube MX-SWETTR --------------------------------- 30 NEW DEEP ULTRAVIOLET OBSERVATION SYSTEM -------------------------------- 42 UV248 compatible intermediate tube U-UVF248IM --------------------------------- 42 UV quartz light guide U-UVF2FV/5FB ------------------------------- 42 UV248 compatible light source box + Mercury Xenon lamp housing U-UVF248LB+U-LH80HBXE ----------------- 42 INTERMEDIATE TUBES & ACCESSORIES ------------------------------- 31 — 32 Magnification changer U-CA ----------------------------------------- 31 Magnification changer 2x U-ECA --------------------------------------- 31 Trinocular intermediate attachment U-TRU --------------------------------------- 31 Dual port U-DP ----------------------------------------- 32 Dual port 1x U-DP1xC ------------------------------------- 32 Eyepoint adjuster U-EPA2 -------------------------------------- 32 Arrow pointer U-APT --------------------------------------- 32 OPTICAL TERMINOLOGY ---------------------------------------------------- 43 — 47 2 WELCOME TO UIS2/UIS OPTICS UIS2/UIS: The System That Maximizes The Advantage Of Infinity-Corrected Optics What's infinity-corrected optics? light between the objective lens and tube lens, allowing the creation of user-specific or taskspecific optical systems. To establish real flexibility with such a system, it is necessary to eliminate the occurrence of coma aberration. UIS2/UIS optics is an infinity-corrected optical system — in other words, a system in which light passes from the specimen through the objective lens without forming an image along the way. Instead, it travels in the form of infinity parallel rays to the tube lens. The tube lens is where the intermediate image is formed, whereas in finitecorrected optics, this is done by the objective lens. *In UIS2/UIS objective lenses, the parfocal distance is designed at 45mm and the focal length of the tube lens is 180mm. Figure 2 Advantages of Infinity-corrected optical system Infinity-corrected optical system Finite-corrected optical system Figure 1 Infinity-corrected and finite-corrected optical system principles Infinity-corrected optical system UIS/UIS2 objective lens Tube lens Finite-corrected optical system Objective lens Objective lens Eyepiece Parallel light beam Intermediate image Tube lens Objective lens Basic dimensions of UIS2/UIS optical system Eyepiece The UIS2/UIS optical system optimally corrects aberration with a dedicated telan lens and an eyepiece so that the coma aberration and flatness are not degraded even when the telan lens exit pupil position is changed by changing the objective lens and telan distance. This makes it possible to use a distance of 50mm to 170mm from objective lens mounting position to the single port tube with lens. Intermediate image Advantages of infinity-corrected optics This system, known as "infinity-corrected optics", offers a number of advantages: *Coma aberration: refer to the optical terminology at the end of • There is no change in magnification even when the distance between the objective lens and tube lens is altered. • With the total magnification remaining constant, there is no image aberration — even when prisms or sliders are interposed between the objective lens and the tube lens. this document. Figure 3 Basic dimensions of UIS2/UIS2 optical system U-TLU (Single port tube with lens) Objective lens * 40mm 45mm As thousands of users have found by experience, these advantages are crucial to composing the ideal microscope optical system. What's more, it is even possible to freely insert or remove intermediate attachments in the parallel rays of Image * 84mm Recommended distance 50-170mm 57.6mm 102mm *Basic dimensions when our revolving nosepiece and illuminator are combined. When the position of the illuminator above is changed, illumination performance cannot be maintained. 3 WELCOME TO UIS2/UIS OPTICS Features of UIS2 objective lenses 4. Lightening Weight has been reduced to approximately 2/3 that of conventional products by using an aluminum objective lens barrel cover. This has the effect of lightening the load on the devices at objective lens up/down, suppressing vibrations by lowering the inertial force at objective lens switching, etc. (MPLFLN series, LMPLFLN series) UIS2 objective lenses ensure compatibility (screw diameter, optical performance) with the UIS optical system and have the following features compared to conventional objective lenses. 1. Wavefront aberration control The Olympus UIS2 objective lenses set a new standard, with wavefront aberration control in addition to common performance standards of N.A. and W.D. Olympus challenges farther highest order optics which has not been fulfilled by the conventional standards. We offer excellent performance objective lenses by minimizing the aberrations that lower resolution. 5. Adoption of eco-lens The glass materials of UIS2 objective lenses are all lead- and cadmium-free eco-glass. Based on our conviction that the UIS2/UIS system is the best way to maximize the advantages of infinity-corrected optical systems, we confidently recommend the UIS2/UIS-featured Olympus microscope units for all your high-precision needs in research, inspection and production equipment. *Wave front aberration: refer to the optical terminology at the end of this document. 2. Objective lenses with excellent image parcentricity High power SemiApochromatic UIS2 objective lenses make the centration tolerance between objective lenses on the microscope nosepiece keep the image within the enter of the field of view even with digital cameras. (50x or higher power in both MPLFLN and LMPLFLN series) * Refer to the Olympus home page for detailed objective lenses specifications. 3. Improvement of color reproducibility UIS2 objective lenses realize natural color reproduction without any chromatic shifts using stringently selected high transmittance glass and advanced coating technology that provides high transmittance which is flat over an ultrawide band wavelength. In addition, since the total optical system, including the tube lens is designed to reproduce a natural color, clear images faithful to the specimen are obtained even with digital imaging. 4 SYSTEM DIAGRAM BXFM SYSTEM DIAGRAM Video system Refer to pages 36-37 Video camera Video camera C-mount B mount 2/3" Video camera S mount 2/3" Video camera F mount U-FMT U-CMAD3 U-BMAD U-SMAD U-TMAD U-TV0.25xC U-TV0.35xC-2 U-TV0.5xC-3 U-TV0.63xC U-TV1x-2 Observation/single tubes and eyepieces Refer to pages 29-30 WHN Eyepieces Widefield trinocular observation tubes SWH Eyepieces Super widefield trinocular observation tubes U-TLU U-TLUIR Illumination systems and power supply U-AN360-3 U-AN360IR U-AN U-PO3 U-POIR U-25ND6, U-25ND25, U-25LBD, U-25IF550, U-25L42, U-25FR, U-BP1100IR, U-BP1200IR U-POTP3 Revolving nosepiece (Refer to pages 34-35) Refer to pages 23-28 FS FS AS 5 AS 6 SHUTTER ND BX-RLA2 BX-URA2 U-KMAS BX-KMA/ BX-KMA-ESD SZX-TLGAD U-RCV LG-SF LG-PS2 U-LGAD Focusing units Refer to pages 19-21 U-DULHA U-LH100-3 U-LH100L-3 U-LH100IR BXFM-ILHS BXFM-ILH TH4-100 TH4-HS U-LH100HGAPO U-LH100HG BXFM-ILHSPU U-LH75XEAPO BXFM-F Stands Refer to page 22 SZ-STL U-ST SZ2-STU2 *Different types may be offered in each area. 5 SYSTEM DIAGRAM MOTORIZED UNIT SYSTEM DIAGRAM Refer to pages 38-40 Observation tubes (Refer to pages 29-30) U-AN U-AN MX-AFDIC U-AN360-3 Intermediate tubes (Refer to pages 31-32) U-FWO Refer to page 26 U-PO3 U-AFA2M-VIS U-LH75XEAPO U-LH100HGAPO U-LH100HG U-POTP3 U-25ND6, U-25ND25 U-25LBD U-25IF550 U-25L42 U-25FR Mirror units U-LH100-3 U-LH100L-3 U-FWR BX-RLAA BX-RFAA U-ZPCB Z board BX-UCB* U-DICR U-D6REMC Refer to page 35 Refer to pages 7-18 See manual U-HSTR2 BF objective lenses U-DICRH BD-M-AD U-D5BDREMC U-D6BDREMC U-DICRHC PC BF/DF objective lenses analySIS FIVE Control software * BX-REMCB is also available for BX-RLAA + motorized revolving nosepiece control (refer to page 39) BXFM-A SYSTEM DIAGRAM Refer to page 41 Refer to page 26 ø32filter Video system (Refer to pages 37-38) Auxiliary lens (provided with the BXFMA-F) Observation tubes (Refer to pages 29-30) MX-AFDIC Power source U-LH100HG U-LH75XEAPO U-AN Refer to pages 27-28 U-AFA2M-VIS LG-SF LG-PS2 U-LGAD Refer to pages 26-27 BXFMA-F U-FWR U-LH100-3 U-LH100L-3 U-RMT BX-UCB U-ZPCB Z board See manual U-D6REMC U-P5REMC U-D5BDREMC U-D6BDREMC U-P6BDREMC Objective lenses U-HSTR2 U-IFFH PC analySIS FIVE Control software U-FH 6 UIS2/UIS OBJECTIVE LENSES ■ Meaning of abbreviations shown on objective lens M P L (Plan) F L N - 1 0 0 M: LM: Metallurgical (no cover) Long working distance metallurgical use SLM: Super long working distance metallurgical use LC: Observation through substrate PL: Plan/ Corrects field curvature of the periphery of the image plane ■ Objective lens notation None: Achromat/ Corrects aberration at 2 wavelengths of blue and red FL: SemiApochromat/ Corrects chromatic aberration in the visible range (violet~red) APO: Apochromat/ Optimally corrects chromatic aberration in the entire visible band (violet~red) B D None: UIS N: UIS2 Number: Objective lens magnification None: Brightfield BD: Brightfield/darkfield BDP: Brightfield/darkfield/ polarizing IR: IR LCD: LCD Objective lenses series abbreviation (PL: Plan) Magnification N.A. (Numerical Aperture) Infinity-corrected optical system For brightfield observation Field Number Cover glass thickness (no cover) ■ Objective lens series list UIS2 UIS UIS2 Series MPLAPON MPLFLN LMPLFLN MPLN LCPLFLN-LCD SLMPLN LMPlanIR/MPlanIR MPLFLN-BD MPLFLN-BDP LMPLFLN-BD MPLN-BD Magnification 50/100 BF \ 1.25/2.5 \ 5/10/20/50/100 5/10/20/50/100 5/10/20/50/100 20/50/100 20/50/100 5/10/20/50/100 *4 5/10/20/50/100/150 5/10/20/50/100 5/10/20/50/100 5/10/20/50/100 \ \ \ \ \ \ \ \ \ \ DF DIC*1 \U POL \ FL F.N. (Field Number) 26.5 1.25x: 22 /2.5x: 26.5 \U \L \ \ \*2 \ \L \ \ \ \ \U aU \L \ a \ \*2 \*2 \ 26.5 26.5 22 26.5 26.5 22 26.5 26.5 26.5 22 Remarks Use together with polarizer and analyzer recommended For LCD For near-IR observation *1 DIC prism U-DICR: UM/LM position, U-DICRHC: LM position fixed, U-DICRH: UM position fixed. *2 5~20x: U excitation also possible *3 50x: DIC observation not applicable *4 MPlanIR: available 100x only \: Responds a: Optimally responds BF: Brightfield DF: Darkfield DIC: Differential Interference Contrast POL: Polarized light FL: Fluorescence ■ Features of objective lens series a MPLAPON: Plan Apochromat — P 8 Plan Apochromat objective lenses that correct chromatic aberrations at the highest level. Olympus guarantees*1 optical performance (wavefront aberration) with a Strehl ratio*2 of 95% or better. They are also designed for use with Olympus' U-AFA2M active AF unit. *1 Definition of guaranteed values: Measurements assessed with Olympus’ Interferometer for Transmitted Wavefront Measurement under specified conditions (measurement: temp. 23°C±1°C; assessment: measurement within the 97% range of the pupil dia.). 2 * Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actual optical system can concentrate with respect to the proportion of light concentrated in the image plane (central intensity) by an ideal, aberration-free optical system, with the latter serving as 100%. A higher percentage indicates a higher quality optical system. a SLMPLN series: Super Long WD M Plan Achromat — P 13 Plan Achromat objective lenses with high magnification and super long working distance. Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses, select from the LMPLFLN Series. a LMPlan-IR series: IR Long WD M Plan Achromat — P 14 MPlan-IR: IR M Plan Achromat — P 14 IR objective lenses which compensate for aberrations from visible to near infrared light. Ideal for the observations of semiconductor interiors and the back surface of a chip package as well as CSP bump inspection. a MPLFLN-BD series: M Plan SemiApochromat BD — P 15 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLFLN-BDP series: M Plan SemiApochromat BDP — P 16 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. The BDP series optimizing brightfield/darkfield and polarized light characteristics is perfect for Nomarski DIC and polarized light observations. a LMPLFLN-BD series: Long WD M Plan SemiApochromat BD — P 17 Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLN-BD series: M Plan Achromat BD — P 18 Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. a MPLFLN series: M Plan SemiApochromat — P 9 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 7 objective lenses ranging from 1.25x to 100x, and secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator. a LMPLFLN series: Long WD M Plan SemiApochromat — P 10 Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLN series: M Plan Achromat — P 11 Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. a LCPLFLN-LCD series: LCD Long WD M Plan SemiApochromat — P 12 Perfect objective lens series for observation of LCD panels and other samples through a glass substrate. Aberration correction matched to the glass thickness is accomplished using a correction ring. 7 UIS2 OBJECTIVE LENSES Plan Apochromat MPLAPON series Plan Apochromat objective lenses that correct chromatic aberrations at the highest level. Olympus guarantees*1 optical performance (wavefront aberration) with a Strehl ratio*2 of 95% or better. They are also designed for use with Olympus' U-AFA2M active AF unit. MPLAPON100x ø20.32 ø20.32 ø8.8 ø5.5 ø16 WD=0.35 WD=0.35 ø18.9 ø20.3 ø28.5 ø20.2 MPLAPON 50x MPLAPON 100x Numerical Aperture 0.95 0.95 Widefield eyepiece WHN10x Field Number 22 Working distance Focal distance f (mm) (mm) 0.35 0.35 Unit: mm ø28 UIS2 objective lenses Objective lens (magnification) *1 Definition of guaranteed values: Measurements assessed with Olympus’ Interferometer for Transmitted Wavefront Measurement under specified conditions (measurement: temp. 23°C±1°C; assessment: measurement within the 97% range of the pupil dia.). 2 * Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actual optical system can concentrate with respect to the proportion of light concentrated in the image plane (central intensity) by an ideal, aberration-free optical system, with the latter serving as 100%. A higher percentage indicates a higher quality optical system. 38.43 42.4 42.78 4.5 45 (44.65) 41 37.98 41.62 (44.65) 45 4.5 MPLAPON50x 3.6 1.8 Weight (g) 139 125 Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm) 500 1000 Screw: W20.32x0.706 (0.8"x1/36") 8 Super widefield eyepiece SWH10x Field Number 26.5 0.44 0.22 1.0 0.67 500 1000 0.53 0.27 1.0 0.7 UIS2 OBJECTIVE LENSES M Plan SemiApochromat MPLFLN series Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 7 objective lenses ranging from 1.25x to 100x, and secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator. MPLFLN2.5x* ø28 ø20.32 ø20.32 4.5 (34) 45 45 ø29 ø30 ø14.5 WD=11 ø20.9 WD=20 ø30 ø20.32 22.55 31.6 ø24.5 WD=10.7 ø29 ø26 MPLFLN20x MPLFLN50x MPLFLN100x ø20.32 ø20.32 ø20.32 38.4 42.4 42.61 45 (44) 36.8 40.8 41.78 45 (44) 35.1 ø10.7 ø7.8 WD=1 WD=1 WD=3.1 ø12.1 ø26 ø26 4.8 4.8 4.5 (41.9) 45 (25) 4.9 45 41.2 (34.3) 4.9 (41.5) 45 WD=3.5 ø28.6 MPLFLN10x 4.5 ø28 ø20.32 MPLFLN5x 28.41 MPLFLN1.25x* ø15.2 ø17.8 ø15.2 ø17.8 ø26 ø26 Unit: mm Widefield eyepiece WHN10x Field Number 22 UIS2 objective lenses Objective lens (magnification) MPLFLN MPLFLN MPLFLN MPLFLN MPLFLN MPLFLN MPLFLN 1.25x* 2.5x* 5x 10x 20x 50x 100x Numerical Aperture 0.04 0.08 0.15 0.30 0.45 0.80 0.90 Working distance Focal distance f (mm) (mm) 3.5 10.7 20.0 11.0 3.1 1.0 1.0 145 72 36 18 9 3.6 1.8 Weight (g) 122 106 51.5 68.1 70.4 89.9 90.9 Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm) 12.5 25 50 100 200 500 1000 Screw: W20.32x0.706 (0.8"x1/36") 9 Super widefield eyepiece SWH10x Field Number 26.5 17.6 8.8 4.4 2.2 1.1 0.44 0.22 870 220 59 15 5.2 1.3 0.73 — 25 50 100 200 500 1000 — 10.6 5.3 2.7 1.3 0.53 0.27 — 220 59 15 5.1 1.3 0.73 UIS2 OBJECTIVE LENSES Long WD M Plan SemiApochromat (WD: Working Distance) LMPLFLN series Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. LMPLFLN10x LMPLFLN20x ø20.32 ø20.32 ø20.32 ø25.4 ø17 ø26 WD=12 ø15.2 WD=21 WD=22.5 29.31 4.8 45 45 45 (33) 22.1 (24) 22.2 (22.5) 4.9 4.5 LMPLFLN5x ø22.4 ø26 LMPLFLN100x ø20.32 ø20.32 37.26 41.1 45 (41.6) 30.4 45 (34.4) 4.9 4.7 LMPLFLN50x ø26 ø12.5 WD=3.4 WD=10.6 ø18.2 ø26 ø15 ø18.1 ø26 Unit: mm Widefield eyepiece WHN10x Field Number 22 UIS2 objective lenses Objective lens (magnification) LMPLFLN LMPLFLN LMPLFLN LMPLFLN LMPLFLN 5x 10x 20x 50x 100x Numerical Aperture 0.13 0.25 0.40 0.50 0.80 Working distance Focal distance f (mm) (mm) 22.5 21.0 12.0 10.6 3.4 36 18 9 3.6 1.8 Weight (g) 50 54 73 77 94 Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm) 50 100 200 500 1000 Screw: W20.32x0.706 (0.8"x1/36") 10 Super widefield eyepiece SWH10x Field Number 26.5 4.4 2.2 1.1 0.44 0.22 70 18 6.1 2.5 0.87 50 100 200 500 1000 5.3 2.7 1.3 0.53 0.27 70 18 6.1 2.5 0.87 UIS2 OBJECTIVE LENSES M Plan Achromat MPLN series Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. MPLN10x MPLN20x ø20.32 ø20.32 ø20.32 41.3 37.2 ø6 ø11.9 ø16 ø15.8 ø24 ø24 MPLN50x MPLN100x ø20.32 ø20.32 38.7 42.8 43.16 45 ø15.8 WD=0.21 ø6 ø11.9 (44.79) 37.2 41.3 42.62 (44.62) 4.5 4.5 42.4 45 ø12 WD=1.3 ø24 (43.7) 28.8 33.6 32.71 4.5 WD=10.6 WD=20 (34.4) 45 23.4 4.5 (25) 45 ø10.5 ø21 WD=0.38 45 4.5 MPLN5x ø4.4 ø11.6 ø15.6 ø24 ø24 Unit: mm Widefield eyepiece WHN10x Field Number 22 UIS2 objective lenses Objective lens (magnification) MPLN 5x MPLN 10x MPLN 20x MPLN 50x MPLN 100x Numerical Aperture 0.10 0.25 0.40 0.75 0.90 Working distance (mm) 20.0 10.6 1.3 0.38 0.21 Focal distance f (mm) 36 18 9 3.6 1.8 Screw: W20.32x0.706 (0.8"x1/36") 11 Weight (g) 64 80 111 113 116 Total Practical field of magnifications view (mm) 50 4.4 100 2.2 200 1.1 500 0.44 1000 0.22 Depth of focus (µm) 98 18 6.1 1.4 0.73 UIS2 OBJECTIVE LENSES LCD Long WD M Plan SemiApochromat LCPLFLN-LCD series Perfect objective lens series for observation of LCD panels and other samples through a glass substrate. Aberration correction matched to the glass thickness is accomplished using a correction ring. LCPLFLN50xLCD*** LCPLFLN100xLCD*** ø20.32 ø20.32 ø20.32 20 27.5 ø17.84 ø25 ø31 ø15.2 WD=0.9 ø29.5 t=0.7 WD=2.5 t=0.7 ø25 ø25 t=0.7 41.6 43.4 45.238 * (43.638) 25 38.65 40.45 (42.038) 45.238 * ø12.77 ø15 WD=7.8 20 4.8 20 24.5 34.75 36.55 4.5 (36.738) 45.238 * 4.5 LCPLFLN20xLCD*** ø29.5 ø31 ø29.5 Unit: mm ø31 * Value at glass thickness 0.7mm observation Objective lens Corresponding glass thickness (mm) Correction ring indication Working distance (mm) Correction system 0 8.3 LCPLFLN20xLCD 0-1.2 0.7 7.8 Correction ring 1.2 7.4 0 3.0 Numerical Aperture LCPLFLN 20xLCD*** 0.45 LCPLFLN 50xLCD*** 0.70 LCPLFLN 100xLCD*** 0.85 Screw: W20.32x0.706 (0.8"x1/36") 1.2 2.2 Widefield eyepiece WHN10x Field Number 22 UIS2 objective lenses Objective lens (magnification) LCPLFLN50xLCD 0-1.2 0.7 2.5 Correction ring Working distance** Focal distance f (mm) (mm) 0 1.2 LCPLFLN100xLCD 0-0.7 0.5 0.98 Correction ring 0.7 0.9 Super widefield eyepiece SWH10x Field Number 26.5 Total Practical field Depth of Total Practical field Depth of Weight magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm) (g) 7.8 9 146 200 1.1 5.2 200 1.3 5.2 2.5 3.6 170 500 0.44 1.6 500 0.53 1.6 0.9 1.8 185 1000 0.22 0.79 1000 0.27 0.79 **The figure shown here is the value when the correction ring indication is 0.7. *** To be available in the beginning of 2007 12 UIS2 OBJECTIVE LENSES Super Long WD M Plan Achromat SLMPLN series Plan Achromat objective lenses with high magnification and super long working distance. Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses, select from the LMPLFLN series. SLMPLN20x SLMPLN50x ø20.32 SLMPLN100x 36.2 37.2 (37.4) 24.1 45 45 ø16.9 ø14.7 ø25 ø17.3 ø23.8 ø26 WD=7.6 ø16.1 WD=18 WD=25 (27) 26.3 19.3 19.8 45 (20) 4.9 ø20.32 4.9 4.9 ø20.32 ø21.4 ø26 ø26 Unit: mm UIS2 objective lenses Objective lens (magnification) Numerical Aperture SLMPLN 20x 0.25 SLMPLN 50x 0.35 SLMPLN 100x 0.6 Screw: W20.32x0.706 (0.8"x1/36") Working distance Focal distance f (mm) (mm) 25 18 7.6 9 3.6 1.8 Weight (g) 56 74 100 Widefield eyepiece WHN10x Super widefield eyepiece SWH10x Field Number 22 Field Number 26.5 Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm) 200 500 1000 13 1.1 0.44 0.22 11.4 4.2 1.3 200 500 1000 1.3 0.53 0.27 11.4 4.2 1.3 UIS OBJECTIVE LENSES IR Long WD M Plan Achromat/IR M Plan Achromat LMPlan-IR series/MPlan-IR UNIVERSAL INFINITY SYSTEM IR objective lenses which compensate for aberrations from visible to near infrared light. Ideal for the observations of semiconductor interiors and the back surface of a chip package as well as CSP bump inspection. LMPlan5xIR LMPlan10xIR LMPlan20xIR ø20.32 30.3 34.3 4.5 45 45 (36.9) 4.5 ø20.32 (26.5) 25 23 22.5 45 (25) 4.5 ø20.32 WD=8.1 ø26 ø10.4 ø12.4 WD=18.5 WD=20 ø21 ø15 ø22 ø18 ø26 ø26 LMPlan100xIR MPlan100xIR ø20.32 ø20.32 ø20.32 37 42.6 43.4 4.5 45 (44.7) 36.8 40.8 45 (41.6) ø12.5 ø15.6 ø18 ø15 ø18.12 ø26 ø4.95 ø12.2 WD=0.3 WD=3.4 36.9 37.5 (39) 32.9 ø12.5 WD=6 45 4.5 4.7 LMPlan50xIR ø14.8 ø26 ø26 Unit: mm Widefield eyepiece WHN10x Field Number 22 UIS objective lenses Objective lens (magnification) LMPlan 5xIR LMPlan 10xIR LMPlan 20xIR LMPlan 50xIR LMPlan 100xIR MPlan 100xIR Numerical Aperture 0.10 0.25 0.40 0.55 0.80 0.95 Working distance (mm) 20.0 18.5 8.1 6.0 3.4 0.3 Focal distance f (mm) 36 18 9 3.6 1.8 1.8 Screw: W20.32x0.706 (0.8"x1/36") 14 Weight (g) 73 73 110 115 122 130 Total magnifications 50 100 200 500 1000 1000 Practical field of Depth of focus view (mm) (µm) 4.4 98 2.2 18 1.1 6.1 0.44 2.2 0.22 0.87 0.22 0.67 UIS2 OBJECTIVE LENSES M Plan SemiApochromat BD (BD:Brightfield/Darkfield) MPLFLN-BD series Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. MPLFLN10xBD MPLFLN20xBD ø26 ø26 ø26 ø22 ø26.2 ø32 35.97 39 39.5 ø22 ø27.5 ø27 ø28.5 ø32 ø32 MPLFLN50xBD MPLFLN100xBD MPLFLN150xBD ø26 ø26 ø26 45 WD=1 41 4.5 ø32 ø20 ø27.2 (44) 41 (44) 45 WD=1 ø20 ø27.2 WD=1 41 4.5 4.5 45 (44) ø17 WD=3 ø29.5 ø16.8 WD=6.5 WD=12 ø16 ø22.4 (42) 45 34.1 37 36.5 (38.5) 45 45 31 30.25 31.5 (33) 4.5 4.5 4.5 MPLFLN5xBD ø32 ø20 ø27.2 ø32 Unit: mm Widefield eyepiece WHN10x Field Number 22 UIS2 objective lenses Objective lens (magnification) MPLFLN 5xBD MPLFLN 10xBD MPLFLN 20xBD MPLFLN 50xBD MPLFLN 100xBD MPLFLN 150xBD Numerical Aperture 0.15 0.30 0.45 0.80 0.90 0.90 Working distance Focal distance f (mm) (mm) 12.0 36 6.5 18 3.0 9 1.0 3.6 1.0 1.8 1.0 1.2 Weight (g) 95.5 82.8 87.7 99.8 98.9 104.8 Screw: W26x0.706 15 Super widefield eyepiece SWH10x Field Number 26.5 Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm) 50 4.4 59 50 5.3 59 100 2.2 15 100 2.7 15 200 1.1 5.2 200 1.3 5.2 500 0.44 1.3 500 0.53 1.3 1000 0.22 0.73 1000 0.27 0.73 1500 0.15 0.6 1500 0.18 0.6 UIS2 OBJECTIVE LENSES M Plan SemiApochromat BDP (BDP:Brightfield/Darkfield/Polarizing) MPLFLN-BDP series Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. The BDP series optimizing brightfield/darkfield and polarized light characteristics is perfect for Nomarski DIC and polarized light observations MPLFLN10xBDP MPLFLN20xBDP ø26 ø26 ø26 35.97 39 39.5 4.5 ø22 ø26 ø32 ø22 ø27.5 ø27 ø28.5 ø32 ø32 MPLFLN100xBDP ø26 ø26 41 45 41 (44) 4.5 MPLFLN50xBDP 4.5 (44) 45 (42) 45 34.1 37 36.5 WD=6.5 WD=12 ø29.3 ø17 ø16.8 ø16 ø22.4 WD=3 45 (38.5) 31 30.25 31.5 45 (33) 4.5 4.5 MPLFLN5xBDP ø32 ø20 WD=1 WD=1 ø20 ø27.5 ø27.5 ø32 Unit: mm Widefield eyepiece WHN10x Field Number 22 UIS2 objective lenses Objective lens (magnification) MPLFLN 5xBDP MPLFLN 10xBDP MPLFLN 20xBDP MPLFLN 50xBDP MPLFLN 100xBDP Numerical Aperture 0.15 0.25 0.40 0.75 0.90 Working distance Focal distance f (mm) (mm) 12.0 36 6.5 18 3.0 9 1.0 3.6 1.0 1.8 Weight (g) 95.5 83.3 88.5 100.5 101.5 Screw: W26x0.706 16 Super widefield eyepiece SWH10x Field Number 26.5 Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (µm) magnifications of view (mm) focus (µm) 50 4.4 59 50 5.3 59 100 2.2 18 100 2.7 18 200 1.1 6.1 200 1.3 6.1 500 0.44 1.4 500 0.53 1.4 1000 0.22 0.73 1000 0.27 0.73 UIS2 OBJECTIVE LENSES Long WD M Plan SemiApochromat BD LMPLFLN-BD series Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. LMPLFLN10xBD LMPLFLN20xBD ø26 ø26 ø26 31.3 32.2 ø22 WD=10 ø22.4 ø28 WD=12 ø15.5 ø16.2 WD=15 32.5 4.5 45 45 (33) 30.2 32 32.3 (35) 24 26.2 26.6 45 (30) 5 4.5 LMPLFLN5xBD ø21.9 ø28.2 ø23 ø28 ø29.5 ø30 ø30 ø32 ø32 ø32 LMPLFLN50xBD LMPLFLN100xBD ø26 36.5 37.3 37.7 (41.7) 45 45 31.8 33 32.7 (34.4) 4.8 4.7 ø26 ø23 ø28 ø21 WD=3.3 WD=10.6 ø20.7 ø32 ø29 ø30.3 ø32 Unit: mm Widefield eyepiece WHN10x Field Number 22 UIS2 objective lenses Objective lens (magnification) LMPLFLN 5xBD LMPLFLN 10xBD LMPLFLN 20xBD LMPLFLN 50xBD LMPLFLN 100xBD Numerical Aperture 0.13 0.25 0.40 0.50 0.80 Working distance Focal distance f (mm) (mm) 15.0 36 10.0 18 12.0 9 10.6 3.6 3.3 1.8 Weight (g) 81 84 86 85 102 Screw: W26x0.706 17 Super widefield eyepiece SWH10x Field Number 26.5 Total Practical field Depth of Total Practical field magnifications of view (mm) focus (µm) magnifications of view (mm) 50 4.4 70 50 5.3 100 2.2 18 100 2.7 200 1.1 6.1 200 1.3 500 0.44 2.5 500 0.53 1000 0.22 0.87 1000 0.27 Depth of focus (µm) 70 18 6.1 2.5 0.87 UIS2 OBJECTIVE LENSES M Plan Achromat BD MPLN-BD series Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. MPLN10xBD ø26 ø26 MPLN20xBD ø26 ø30.5 41 ø17 WD=1.3 WD=6.5 ø16.8 ø29.3 WD=12 42.5 45 ø16 (43.7) 34.01 37 36.5 45 (38.5) 31 30.25 31.5 45 (33) 4.5 4.5 (4.5) MPLN5xBD ø22 ø26 ø32 ø23.6 ø29 ø27 ø32 ø32 MPLN100xBD ø26 ø26 41 42.5 43.33 43.71 45 (44.79) 41 42.5 43.68 45 (44.62) 4.5 4.5 MPLN50xBD ø10 ø23.1 WD=0.21 WD=0.38 ø10 ø20.7 ø23 ø27 ø29 ø29 ø32 ø32 Unit: mm Widefield eyepiece WHN10x Field Number 22 UIS2 objective lenses Objective lens (magnification) MPLN MPLN MPLN MPLN MPLN 5xBD 10xBD 20xBD 50xBD 100xBD Numerical Aperture Working distance (mm) Focal distance f (mm) Weight (g) 0.10 0.25 0.40 0.75 0.90 12.0 6.5 1.3 0.38 0.21 36 18 9 3.6 1.8 137 155 162 157 160 Screw: W26x0.706 18 Total Practical field of magnifications view (mm) 50 100 200 500 1000 4.4 2.2 1.1 0.44 0.22 Depth of focus (µm) 98 18 6.1 1.4 0.73 MICROSCOPE SYSTEM BXFM BXFM frame BXFM-F Widely used system that allows use in combination with fiber illumination, motorized revolving nosepiece and telan lens unit. Can easily be integrated into other equipment. Attach to the equipment by rear bolt mounting screw or pillar mounting hole. 84 36 82 4–M4 depth9 (13) Stroke 35 0.5 0.5 4–M4 depth7 66.2 16 17 Pillar mount hole center (13) 17 80 55 124 100 17 34 23 4-M8 depth8 (Bolt mount screw) 7 58.5 36 2A00002 ø3 2H (Pi 8 llar mo un 98 th Weight: 1.9kg 19 ole ) 110 Unit: mm MICROSCOPE SYSTEM BXFM BXFM BXFM-F+BXFM-ILH+BXFM-ILHSPU 180 Accommodates the reflected light brightfield/darkfield and fluorescence illuminators. Light axis 165 Pillar axis 130 Holder mounting position Revolving nosepiece mounting position Objective lens mounting position 11 45 72 23 40 Stroke 7 3.5 Light axis 83 Specimen position Weight: 3.2kg Unit: mm BXFM combination sample BXFM-F+BXFM-ILH+BXFM-ILHSPU+TR30-2+BX-RLA2+U-LH100L-3 165 130 87.5 83 17-47(stroke) 124 45 11 40 3.5 249 ø 32 220 Specimen surface 180 587 * For installation dimensions, refer to those for the BXFM-F (page 19). Weight: 8.2kg (exclude objective lens) 20 Unit: mm MICROSCOPE SYSTEM BXFM BXFM-S BXFM-F+BXFM-ILHS 84 Compact focusing unit suitable for building into existing equipment. 141 106 Pillar axis Holder mounting position Light axis Stroke Revolving nosepiece mounting position 45 Objective lens mounting position 23 40 20 7 59 Weight: 2.4kg Unit: mm Specimen position BXFM-S combination sample BXFM-F+BXFM-ILHS+TR30-2+U-KMAS+U-LH100L-3 290 187 92.5 106 19-49 (Stroke) 169 20 45 40 124 ø32 Specimen surface 84 208 * For installation dimensions, refer to those for the BXFM-F (page 19). Weight: 5.5kg (exclude objective lens) 21 Unit: mm MICROSCOPE SYSTEM BXFM Stands A wide variety of stands are available to suit different applications and purposes. SZ2-STU2 Universal stand type 2 250 300 Major specifications Item 1 Diameter of focusing arm or fixing section of tube 2 Vertical pole diameter 3 Horizontal poles diameters 4 Stroke 5 Movement range 300 350 6 Maximum specimen weight 622 º 7 Weight ø40mm ø25mm (both upper and lower poles) Horizontal: 234mm, Vertical: 205mm Horizontal: 541 (435+106) mm max. (Vertical pole — BXFM-S optical axis) Forward: 10kg (within 90-degree area) Transverse direction: 6kg Backward direction: 7kg (at maximum stroke) 30kg 90 30 ø25 180º 214~435 Specifications ø32mm 503 Ø32 ø40 50.5 130 * The rotation angle of the horizontal arm can restrict to 90 degrees with stopper. U-ST Compact stand 160 133 320 SZ-STL Large stand 88 ø110 400 35 106 73 ø32 143 17.5 45 20 49 46 133 267 ø32 Weight: 1.8kg Weight: 5kg 22 Unit: mm ILLUMINATION UNITS Reflected light illuminator for BF/DF BX-RLA2 ND filters are linked when exchanging between brightfield and darkfield. Accessories 35 16.2 12 29.4 Revolving nosepiece mounting position Illuminator mounting position 13 11.8 17 26 79 50 130 130 130 17.5 ø75 88 107.5 46 108 7.5 38.7 56.8 41 U-DICRHC Weight (g) 20 20 20 20 20 20 71 71 84 U-AN360-3 U-AN U-DICR U-DICRH Description LBD filter slider IF550 filter slider ND filter ND filter Frost filter slider UV-cut filter Polarizer slider for reflected light Polarizer slider for reflected light with tint plate 360° rotatable analyzer slider Analyzer slider for reflected light DIC slider for reflected light DIC slider for reflected light (high resolution type) DIC slider for reflected light (high contrast type) 3.5 Unit name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 30 31.5 30 144 45 265 335 Weight: 3.4kg Universal reflected light illuminator BX-URA2 Suitable for observations ranging from brightfield to fluorescence. Six mirror units can be attached to this reflected light illuminator simultaneously. Accessories U-MWUS3 U-MWBS3 U-MWGS3 ø84 88 (152) 11.6 11.6 12 17 76 86.8 27.2 80 Revolving nosepiece mounting position 41 80 80 80 80 84 130 3.5 U-MBF3 U-MDF3* U-MDIC3 U-MBFL3 79 50 130 130 ) U-DICRHC Weight (g) 20 20 20 20 20 20 71 71 .9° U-AN360-3 U-AN U-DICR U-DICRH Description LBD filter slider IF550 filter slider ND filter ND filter Frost filter slider UV-cut filter Polarizer slider for reflected light Polarizer slider for reflected light with tint plate 360° rotatable analyzer slider Analyzer slider for reflected light DIC slider for reflected light DIC slider for reflected light (high resolution type) DIC slider for reflected light (high contrast type) Mirror unit for reflected brightfield Mirror unit for reflected darkfield Mirror unit for reflected DIC Mirror unit for reflected brightfield, for high intensity light source Fluorescence mirror unit for reflected (U excitation) Fluorescence mirror unit for reflected (B excitation) Fluorescence mirror unit for reflected (G excitation) (17 Unit name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 5° 41 13 Illuminator mounting position 261 367 80 Weight: 3.8kg 80 * U-RCV (DF converter for BX-URA2) is needed with darkfield observation. Unit: mm 23 ILLUMINATION UNITS Reflected light illuminators for BF BX-KMA/BX-KMA-ESD Enables brightfield, Nomarski DIC and simple polarizing observations. ESD model is also available. U-DICRHC 130 Revolving nosepiece mounting position ø70 41 79 50 130 130 108 Weight (g) 20 20 20 20 20 20 71 71 84 U-AN360-3 U-AN U-DICR U-DICRH Description LBD filter slider IF550 filter slider ND filter ND filter Frost filter slider UV-cut filter Polarizer slider for reflected light Polarizer slider for reflected light with tint plate 360° rotatable analyzer slider Analyzer slider for reflected light DIC slider for reflected light DIC slider for reflected light (high resolution type) DIC slider for reflected light (high contrast type) 3.5 Unit name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 88 Accessories Cable length 260.5mm Illuminator mounting position 30 250 312.5 * Combine SZX-TLGAD when using fiber illumination. Weight: 3.1kg Unit: mm MOUNTING DIMENSIONS OF ILLUMINATORS (BX-RLA2, BX-URA2 and BX-KMA/BX-KMA-ESD) 100 (±0.1) 21 36 (±0.1) 170 (Distance to the light axis) 2 5.5 52 (Revolving nosepiece relief dimension) 4-M5 depth12 or more 62 (±0.1) (6) (11) Location face 37 ±0.1 (45° location face) 45° 2.5 (±10 ') 5 3.5 Location face ss Le an th R6 82 (Revolving nosepiece relief dimension) Convex section(2-4) for positioning Fix illuminator using four M5 screws and projection for fastening. Unit: mm 24 LAMP HOUSING & ACCESSORIES Reflected light illuminator for BF U-KMAS Very compact reflected light illuminator with reduced depth. U-DICRHC 88 Weight (g) 20 20 20 20 20 20 71 71 64 79 50 130 130 21 U-AN360-3 U-AN U-DICR U-DICRH Description LBD filter slider IF550 filter slider ND filter ND filter Frost filter slider UV-cut filter Polarizer slider for reflected light Polarizer slider for reflected light with tint plate 360° rotatable analyzer slider Analyzer slider for reflected light DIC slider for reflected light DIC slider for reflected light (high resolution type) DIC slider for reflected light (high contrast type) 6 Unit name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 ø75 Accessories 155 198 130 21 Weight: 1.2kg Unit: mm 25 LAMP HOUSING & ACCESSORIES Lamp housings Various different lamp housings are available, for use with different light sources: choose to suit the intended purpose. * If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand. U-LH75XEAPO 75W xenon apo lamp housing (148.5) 30.2 (148.5) 83.5 65 65 U-LH100HGAPO 100W mercury apo lamp housing U-LH100HG 100W mercury lamp housing (30.2) 83.5 65 93 65 93 8 8 130 70 75 75 55 20 6 20 40.8 40.8 115 30° 25° 115 30° 25° 169 (depth dimension for installation) 130 180.5 18.5 169 (depth dimension for installation) 180.5 Cable length 2,000mm Accepted lamp: UXL-75XB Weight: 3.1kg Cable length 2,000mm Accepted lamp: USH-103OL Weight: 2.7kg * Power supply unit (U-RFL-T200) and power cable (UYCP) are necessary for 100W mercury lamp housings. These items are sold separately. U-RFL-T200 dimensions: 150(W)x295(D)x200(H), weight approx. 4.8kg *Power supply unit (U-RX-T200) and power cable (UYCP) are necessary for 75W xenon lamp housing. These items are sold separately. U-RX-T200 dimensions: 115(W)x195(D)x260(H), weight approx. 3kg 107 U-LH100-3/U-LH100IR/U-LH100L-3 100W halogen lamp housings Weight: 880g Accepted lamp: 12V100WHAL (high intensity lamp) 12V100WHAL-L (long life lamp) 37 85.5 Cable length U-LH100-3: 290mm U-LH100IR: 290mm U-LH100L-3: 800mm 135(depth dimension for installation) 146.5 * External power supply (TH4-100 or TH4-200) and power cable (UYCP) are necessary for 100W halogen lamp housings. These items are sold separately. For TH4-100/200 installation dimensions, refer to the next page. Unit: mm 26 LAMP HOUSING & ACCESSORIES Lamp housing accessories For the 100W halogen lamp, the external power supply TH4-100/200 with an intensity adjustment switch and an ON/OFF switch, both are located close to the operator's hand, are provided. All Olympus reflected light illuminators can be used with fiber illumination. * If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand. TH4-100/200 External power supply TH4-HS Hand switch Cable length: 2,000mm 77 120 125 18.5 Weight: 2.2kg Weight: 140g 42 75 14.5 38 200 U-RMT Extension cord 1,700 37.5 21 Darkfield light excluding tube which is built into the BX-URA2. ø12 ø30 26 31.5 (42.5) 51 (dimensions for installation) Weight: 315g 62.5 (dimension for installation) Light guide mount hole ø12 Weight: 390g 24 44 ø49 SZX-TLGAD Transmitted light guide adapter ø32 ø67 ø59 ø75 U-LGAD Fiber adapter for reflected light observation ø59 43 U-RCV DF converter for BX-URA2 Weight:200 g (dimension for installation) * Mountable with BX-KMA/BX-KMA-ESD only. Light guide mount hole ø12 Weight: 135g Unit: mm 27 LAMP HOUSING & ACCESSORIES LG-PS2* Light source ø15 (Light guide mounting position) 8 235 10 86 130 76 10 126 251 *The types of model varies by country in use. Weight: 1.6kg LG-SF Light guide Groove: Width3, Depth1 61 ø13 ø25 30 31 ø10.1 ø12 ø15 10 25 20 1,000 Weight: 210g U-DULHA Double lamp house adapter 82 88 202 ø14 171 0 Weight: 1.2kg 28 Unit: mm OBSERVATION TUBES Widefield trinocular observation tubes Trinocular observation tubes with widefield of view. Compatible with F.N. 22. U-ETR-4 Widefield erect image trinocular tube 60.6 62.5 103.9 104.9 92 47.9 62.5 (IR: 64.5) 150.5 186.6 (IR: 188.9) 95.8 18 92.5* (IR: 93.9) 59.65 33.5 16 120 175 U-TR30-2/ Widefield trinocular tube U-TR30IR Widefield trinocular tube for IR 43.5 163.1 199.9 51.6 Unit: mm Name U-TR30-2 U-TR30IR U-ETR-4 Field Number (F.N.) Inclination angle (degree) Interpupillary distance (mm) Light path selector (eyepiece/video port) Observation image Weight (g) 22 22 22 30 30 30 50-76 50-76 50-76 100/0, 20/80, 0/100 100/0, 0/100 100/0, 0/100 Inverted Inverted Erect 1600 1600 1900 *Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62mm. Single port tube with lens When the visual observation is not needed and only video observation is required, a single port tube with a built-in telan lens can be attached directly to the video port. U-TLU Single port tube with lens U-TLUIR Single port tube with lens for IR 56 ø60 Weight: 350g • For attachable video camera adapters, refer to video camera adapters system diagram page (pages 5-6). Unit: mm 29 OBSERVATION TUBES Super widefield trinocular observation tubes Trinocular observation tubes with super widefield of view. Compatible with F.N. 26.5. U-SWETR Super widefield erect image trinocular tube 224 76.4* 55.6 79.6 98.8 68.6 173.8 220.8 73 83.4 92.9 36.5 19 14 82.3* 63.1 180.8 U-SWTR-3 Super widefield trinocular tube 49.9 98.6 62 201.9 248.9 139.400 61.981 117.223 MX-SWETTR Super widefield erect image tilting trinocular tube 88.000 95.929 101.929 149.081 2.837 70.330 20° 0' 0" 42° 0' 0 " 318.527 Name U-SWTR-3 U-SWETR MX-SWETTR Field Number (F.N.) 26.5 26.5 26.5 Inclination angle (degree) 24 24 0-42 328.338 337.718 Interpupillary distance (mm) 50-76 50-76 50-76 72.200 Unit: mm Light path selector (eyepiece/video port) 100/0, 20/80, 0/100 100/0, 0/100 100/0, 0/100 *Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62mm. 30 Observation image Inverted Erect Erect Weight (g) 2300 4200 4200 INTERMEDIATE TUBES & ACCESSORIES Intermediate tubes Various accessories for various observation need. U-ECA Magnification changer 2x Provides 1x and 2x intermediate magnifications. ø138 U-CA Magnification changer Provides 1x, 1.2x, 1.6x and 2x intermediate magnifications. 150 ø140 88 96 42 42 45 37 ø75 Weight: 1.3kg 52 ø70 Weight: 1.3kg U-TRU Trinocular intermediate attachment Intermediate attachment which divides the light path, allowing attachment of both digital and video cameras. 37 150 ø140 183.9 52 58.2 106.9 BI:PT=100:0/20:80 Weight: 1.3kg 31 Unit: mm INTERMEDIATE TUBES & ACCESSORIES U-DP Dual port Use this intermediate tube to divide the light path. U-DP1xC Dual port 1x Combine with U-DP to obtain a 1x image. ø140 88 ø44 ø30 151 ø44 ø25 17.53 1-32UN 4.5 170.5(mount face) 182 Weight: 500g 57 38 51(mount face) Weight: 1kg Light path selector by mirror unit Transmitted side port: side port = 70:30 (with use of U-MBF3) 21.2 89 (8°) U-EPA2 Eyepoint adjuster Raises eyepoint by 30mm. 115 U-APT Arrow pointer Projects an arrow into the field of view. Transmitted side port: side port = 100:0 (45°) 45 88 15V0.2A 30 0.92 (tolerence from light axis) 120 45.3 Weight: 1.2kg Weight: approximately 500g Unit: mm 32 EYEPIECES/FILAR MICROMETER EYEPIECE Eyepieces Eyepieces for UIS2 optical system. WHN10x Widefield eyepiece WHN10x-H CROSSWHN10x Widefield eyepieces WH15x Widefield eyepiece SWH10x-H MICROSWH10x CROSSSWH10x Super widefield eyepieces ø46.2 ø43.2 ø39 ø41 ø36.5 EP 36.8 53.2 18.7 29.6 48.6 27.8 (28.5) ø30 ø30 ø30 Name Field Number WHN10x WHN10x-H CROSSWHN10x WH15x SWH10x-H MICROSWH10x CROSSSWH10x *EP=eyepoint 22 22 22 14 26.5 26.5 26.5 Diopter adjustment range (1/m) — -8 — +5 -8 — +5 — -8 — +2 -8 — +2 -8 — +2 ø30 Weight Micrometer diameter (mm) (g) 24 24 — 24 — — — 90 170 170 90 210 210 210 69.9 (23.2) (23.1) (25) 43.7 62.6 39.4 51.2 28 39.6 48.6 EP 60.2 ø41 ø38.5 41.4 ø41 ø38.5 Remarks With adjustable diopter With cross lines and adjustable diopter With adjustable diopter With micrometer and adjustable diopter With cross lines and adjustable diopter Unit: mm Filar micrometer eyepiece U-OSM Used for precise measurement in the field of view. 23.5 Eyepiece 30 65 41 137 129.2 117.8 EP Magnification 10✕, erect image (inverted when used with erect image observation tube), F.N. 14. Diopter adjustment range: ±5 1/m. Provided with rubber eye shade. Measuring scale Scale lines graduated in increments of 1mm in the entire 10mm length. Shift of scale lines: 1mm per rotation of the shift ring, the circumference of which is divided into 100 graduations. 60.9 Measuring range 10mm/objective lens magnification Mounting position (inside) Compensation limit for objective lens magnification tolerance Actual size 29.5 ±5% by combined use of the zoom compensation ring and the provided stage micrometer. Compensation ring clamping screw. Magnification compensation scale. Actual size (mm) = Measured value (mm) Objective lens magnification 75.5 Weight: 580g Repeatability 0.007 mm A (A … Objective lens magnification) Accuracy *Measuring error (A … Objective lens magnification: L … Measured length in mm) 0.007 ±[ (0.0002✕A+0.002) L + ] mm A Repeatability error ± Unit: mm 33 REVOLVING NOSEPIECES Revolving nosepieces for BF objective lenses Choose from following 6 types. For motorized nosepieces, refer to motorized unit page. U-D7RE Septuple revolving nosepiece with slider slot for DIC 104 116.5 U-D6RE Sextuple revolving nosepiece with slider slot for DIC U-D6RE-ESD Sextuple revolving nosepiece with slider slot for DIC with ESD treatment 83 U-5RE-2 Quintuple revolving nosepiece (87.4) (125.6) 60.9 87.6 76.4 47.2 40 48.2 40 38 40.8 38 (114.4) 40 26.5 .4 ø102 ø116.5 ø84 Weight: 520g Weight: 800g Weight: 980g U-P6RE Centerable sextuple revolving nosepiece with slider slot for DIC 104 116.5 U-P4RE Centerable quadruple revolving nosepiece with slider slot for DIC (125.6) (114.4) 76.4 87.6 40 47.2 38 40 48.2 38 .4 ø102 .5 ø116 Weight: 1kg Weight: 1kg Insert the DIC dummy when not using the DIC slider 34 Unit: mm REVOLVING NOSEPIECES Revolving nosepieces for BF/DF objective lenses Choose from following 3 types. Use of adapter to mount BF objectives (BD-M-AD) enables attachment of brightfield objective lenses. For motorized nosepieces, refer to motorized unit page. U-D6BDRE Sextuple revolving nosepiece for BF/DF with slider slot for DIC/ U-P5BDRE Centerable quintuple revolving nosepiece 2A00002 JAPAN 104 U-D6BDRE U-D6BDRE 116.5 U-D5BDRE Quintuple revolving nosepiece for BF/DF with slider slot for DIC 104 U-5BDRE Quintuple revolving nosepiece for BF/DF N PA JA (125.6) 87.6 47.2 40 76.4 .4 ø102 .4 ø102 .5 Ø116 Weight: 800g Weight: 1kg Insert the DIC dummy when not using the DIC slider BD-M-AD Adapter to mount BF objectives W26✕0.706 ø28.2 +0.2 0 W20.32✕0.706 ø30 Weight: 800g 38 40 38 48.2 40 48.2 34.8 (114.4) (111.2) 76.4 Weight: 10g (4) 4 Unit: mm 8 35 VIDEO CAMERA ADAPTERS C-mount video camera ports Allows direct attachment of a C-mount video camera. Four types are provided: 0.63x, 0.5x, 0.35x and 0.25x. All models feature a focus adjustment function U-TV0.25xC C-mount video port with 0.25x lens 17.53 ø36 1-32UN 3.5 Image plane U-TV0.35xC-2 C-mount video port with 0.35x lens 1-32UN 4 12.4 22.4 147.3 156.84 17.53 Image plane ø60 ø60 ø64 Weight: 1.2kg Weight: 100g U-TV0.5xC-3 C-mount video port with 0.5x lens U-TV0.63xC C-mount video port with 0.63x lens ø30 1-32UN 3.5 17.53 Image plane 1-32UN 68.75 78.25 3.5 ø30 30.1 32.6 42.1 17.53 Image plane 2 A0 0 0 0 1 ø60 ø60 Weight: 200g Weight: 430g Unit: mm Field of view (F.N.) 2/3" CCD 1/2" CCD ]Video camera adapter (Projection lens) Projection area (F.N.) Projection magnifications 2/3" CCD 1/2" CCD 1x 11 8 6 0.63x 17.5 12.7 9.5 U-TV0.5xC-3 0.5x 22 16 12 U-TV0.35xC-2 0.35x — 22 17.1 U-TV0.25xC 0.25x — — 24 U-TV1x-2 U-TV0.63xC Practical field of view (mm) = 1/3" CCD Projection area (Field Number) Objective lens magnifications Projection area Focus the video camera adapter to prevent defocusing the eyepiece image and defocusing by magnification switching. Generally, the video camera adapter is focused by switching to a low magnification after focusing at a high magnification.objective lens. 36 VIDEO CAMERA ADAPTERS Video camera mount adapters Allows attachment to video cameras with C, Bayonet, Sony and F mounts. Use with the U-TV1x-2. Focus by amount of screwing into U-TV1x-2. U-CMAD3 C-mount adapter U-BMAD Bayonet mount adapter U-SMAD Sony mount adapter ø44.5 ø30 1-32UN Image plane Image plane M56X2 ø48 40 60 3 30 M56X2 49 60.5 80.5 20 48.7 ø42 4 48 38 4 17.53 Image plane M56X2 ø64.4 ø64 ø64 Weight: 165g Weight: 80g Weight: 90g U-TMAD T mount adapter U-FMT F/T mount adapter * Image plane 46.5 55 Image plane M42X0.75 ø45.7 23 43 13 4 ø42 ø54.7 M56X2 ø64 Weight: 70g Weight: 30g * It must be combined with U-TMAD Unit: mm Video camera port This port can be attached directly to the trinocular observation tube as well as to the single port tube with lens. U-TV1x-2 Video port 1x 28 22 ø64 ø60 Weight: 150g Unit: mm 37 MOTORIZED UNITS Motorized units Various motorized units, perfect for automation of equipment, are available. 11.8 12 115 108 107 80 BX-RLAA+U-D6REMC+U-LH100-3 Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple revolving nosepiece+100W halogen lamp housing Enables motorized exchange of objective lenses, selection between brightfield and darkfield observations as well as aperture diaphragm closing/opening. The BX-UCB control unit has an RS232C connector, allowing control via a PC. For method of attaching illuminator, refer to page 24. 86.2 (487) 400 64.8 45 81.5 40 (169) 41 211.5 84 87 Illuminator cable length: 1.800mm Weight: 5.5kg(exclude objective lens) 88 11.6 87.5 27.2 41 Revolving nosepiece mounting position 126 109.5 86.8 11.6 17 12 76 ø84 14 92 15 BX-RFAA Motorized universal reflected light illuminator Reflected light fluorescence illuminator with simultaneous attachment of six mirror units. Incorporates motorized mirror unit changeover and shutter. Illuminator mounting position Illuminator cable length: 1.800mm 135 Weight: 4.3kg 41 261 371 38 Unit: mm MOTORIZED UNITS U-D6BDREMC Motorized sextuple BD revolving nosepiece with slider slot for DIC U-P5BDREMC Motorized centerable quintuple BD revolving nosepiece with slider slot for DIC U-D5BDREMC Motorized quintuple BD revolving nosepiece with slider slot for DIC U-D6REMC Motorized sextuple revolving nosepiece with slider slot for DIC U-P5REMC* Motorized centerable quintuple revolving nosepiece with slider slot for DIC 34 115 14 50 46 115.5 46 103.5 135 150 Weight: 1.4 kg 40 *Please contact Olympus for the availability. Weight: 1.1kg BX-UCB Control unit Motorized units including motorized illuminator and auto focus unit can be totally controlled from BX-UCB U-HSTR2 Hand switch 108 32 212 216 50 68.3 40 51.1 69.8 40.5 25.5 40.5 25.5 150 105 50 14 34 74.5 83.5 103.5 135 7° 146 Cable length 2000mm Weight: 370g 310 125 332 (depth) Weight: 1.0kg * Extension cord U-RMT (1700mm) should be used to connect the lamp housing (U-LH100-3) to the BX-UCB. BX-REMCB Control box for motorized nosepiece and BF/DF illuminator BX-RLAA and U-D5BDREMC/U-D6REMC/U-P5REMC can be controlled from U-HSTR2, or direct from the computer keyboard via an RS232C connector. * BX-RFAA and U-D5BDREM/U-D6REM combination not applicable. U-ACAD4515 AC adapter for BX-REMCB 2000 +100 0 39.8 34 144 71±1 190.4 35±1 129.5±1 39 Unit: mm MOTORIZED UNITS U-AFA2M-VIS Active auto focus unit Featuring an AF laser light source in wavelength 785 nm. The multiple-spots sensor enables the high-speed and stable focusing of specimens with variable height differences. 108 313 45 Weight: 2.6kg Weight: 0.23kg Weight: 0.36kg length: 2m length: 3m * Consult your Olympus dealer about the motorized focus. U-FWR Motorized reflected filter wheel Accomplish maximum 6 filter position exchange 180.5 Weight: 1.0kg 24.5 58.5 130 147.9 42 Cable AFA2M-CBL2M AFA2M-CBL3M 30.5 51 62.5 2000 Unit: mm 40 MOTORIZED UNITS 133 45 56 310.5 206 77 BXFMA-F Motorized illumination with power focus A motorized microscope unit for integration with your equipment. Motorized operations such as revolving nosepiece up/down, objective lens switching, aperture diaphragm open/close, and brightfield/darkfield switching are accomplished with this component. Several microscopic operations are totally controlled from an external unit by combining this component with an auto focus unit. This is the configuration combined with Active Auto Focus Unit U-AFA2M-VIS, Single Port Tube with Lens U-TLU, a lamphousing, a motorized nosepiece and objective lenses. 36 169 32.5 Stroke Specimen surface (standard focusing position) 2 51 7 77 334 342.1 92 * Consult your Olympus dealer about the mounting dimensions. U-FH Focus adjustment knob unit Weight: 13kg (BXFMA-F frame 7.6kg) U-IFFH Focus adjustment knob interface 70 3 50 100 104 91.5 33.6 Weight: 760g 70 75.5 4 210 54 82.3 214 Weight: 1450g Unit: mm 41 DEEP ULTRAVIOLET OBSERVATION SYSTEM Deep ultraviolet observation system This module adds a deep ultraviolet (248nm) optical system to a general microscope. An ultra-high resolution observation is executed by using an extremely short wavelength ray. U-UVF2FB/5FB UV quartz light guide 2000 +200 0 or 5000 +200 0 79 69 30.4 108 34 ø8 (light guide) U-UVF248IM UV248 compatible intermediate tube Weight: U-UVF2FB 50g U-UVF5FB 80g 105.8 258.5 ø39.4 196.8 20 (39.5) 5.5 ø70 6 196 Weight: 1.9kg U-UVF248LB+U-LH80HGXE UV248 compatible light source box + Mercury Xenon lamp housing 45 180 210.3 170 150 150 30 4-ø4.5, ø8 C'bore 5 Deep 227 8 4-ø4.5, ø13 C'bore 5 Deep 170 45 (distance between mounting positions) 1-32 UNF (C Mount Thread) 157.5 102.5 ø30 240 41 Weight:6.5kg 42 Unit: mm OPTICAL TERMINOLOGY 1. Field Number (F.N.) and Practical Field of View 5. Total Magnification The field number (F.N.) is referred to as the diaphragm size of eyepiece in mm unit which defines the image area of specimen. The diaphragm diameter actually seen through eyepiece is known as the practical field of view (F.O.V.) which is determined by the formula: 5.1 Observation through eyepiece (binocular observation) F.O.V. = M(bino)=M(ob)×M(oc) M(bino): Total magnification for binocular observation M(ob): Objective lens magnification M(oc): Eyepiece magnification Eyepiece F.N. (mm) Objective lens magnification 5.2 Video monitor observation ● Total magnification for video monitor M(video monitor)=M(ob)×M(video camera adapter)×Monitor magnification* M(video monitor): Total magnification on the video monitor M(ob): Objective lens magnification M(video camera adapter): Projected magnification for video camera 2. Working Distance (W.D.) The distance between the front edge of the objective lens and the specimen surface (with the surface of the cover glass in case of the cover glass objective lens) when the specimen is focused. adapter including photo eyepiece (refer to Figure 1) * Refer to Figure 3 for "Monitor magnification" ● Practical field of view for video monitor observation 3. Parfocal Distance Practical field of view for = video monitor observation It is the distance between the objective lens mounting plane and the specimen. In UIS2/UIS objective lenses, the parfocal distance is designed at 45mm. Working distance and parfocal distance Image device size * M(ob)×M(video camera adapter) M(ob): Objective lens magnification M(video camera adapter): Projected magnification for video camera adapter including photo eyepiece Objective lens mounting position (refer to Figure 1 for projected magnifications) * Refer to Figure 2 for image device size Figure 1 Video camera adapter and projection magnifications Video camera adapter (Projection lens) U-TV1x-1 + video camera mount adapters Parfocal distance Working Distance (W.D.) Projection magnifications 1x U-TV0.63xC U-TV0.5xC-3 U-TV0.35xC-2 U-TV0.25xC 0.63x 0.5x 0.35x 0.25x Focal plane Figure 2 Imaging device size Camera format 1/3" 1/2" 2/3" For parfocal distance of the LCPLFLN-LCD series objective lenses, refer to the appropriate objective lens page. Diagonal 6.0mm 8.0mm 11.0mm Horizontal 4.8mm 6.4mm 8.8mm Vertical 3.6mm 4.8mm 6.6mm The above table is for standard image device sizes. Check your device size for precise calculation. 4. Relationship between the objective lens's focal length and magnifications Figure 3 Imaging device size and monitor magnifications Monitor size (diagonal) Camera format 10" 15" 17" 19" 1/3" 42.3x 63.5x 72.0x 80.4x 1/2" 31.8x 47.6x 54.0x 60.3x 2/3" 23.1x 34.6x 39.3x 43.9x Indicated magnifications of UIS2/UIS objective lenses are the values when the focal length of the tube lens is 180 mm. M(ob)= Focal length of tube lens f 21" 88.9x 66.7x 48.5x Example What is total magnifications for video monitor when objective lens is 50x, video camera adapter U-TV0.5xC, 2/3" video camera and 21" monitor are used ? M(ob): Objective lens magnification f: Objective lens's focal length 43 OPTICAL TERMINOLOGY •Total magnification on the video monitor: m(ob)=50×, M(video camera adapter) is 0.5× from Figure 1 and monitor magnification is 48.5× from Figure 3. M(monitor observation)=M(ob)×M(video camera adapter)×monitor magnification =50×0.5×48.5=1213× ● Resolving power formula The following formula is generally used for determing resolution. λ (Reyleigh formula) ε = 0.61 × N.A. λ: Wavelength or radiation in use (λ=0.55µm is used for visible light) N.A.: Objective lens N.A. •Practical filed of view for video observation(horizontal side): M(ob)=50×, M(video camera adapter) is 0.5× from Figure 1 and horizontal side of 2/3" imaging device is 8.8mm from Figure 2 Practical field of view = for video observation Example MPLFLN100×(N.A.=0.90), λ=0.55µm Image device size M(ob) × M(video camera adapter) ε = 0.61 × 8.8 (mm) = =352µm 50 × 0.5 λ 0.3355 0.3355 = = = 0.37µm N.A. N.A. 0.90 8. Focal depth of Microscope 6. Numerical Aperture (N.A.) The focal depth refers to the depth of the specimen layer which is in sharp focus at the same time, even if the distance between the objective lens and the specimen plane is changed when observing and shooting the specimen plane by microscope. As human eyes are individually different in the ability of their focus adjustment, each person's perception of the focal depth varies. At present, the Berek formula is generally used, because it gives a focal depth value that often coincides with that obtained through experiments. The numerical aperture is a key factor to the performance of objective lens (resolving power, focal depth and brightness). The N.A. is determined by the following formula: N.A.= n × sinθ n=Refraction rate of the medium between specimen and objective lenses. (Air: n=1, oil: n=1.515) θ: Angle which is made by the optical axis and refraction of the light farthest from the center of the lens. Focal depth formula ● Visual observation (Berek formula) The visual field brightness (B) of the microscope is determined by the following formula in relation to the objective lens magnification (M). The larger the N.A. and the lower the objective magnification, brightness will increase in the factor of the second power. ± D.O.F.= D.O.F.: Depth Of Focus N.A.2 B∝ M2 ω: Resolving power of eyes 0.0014 (when optical angle is 0.5 degrees) M: Total magnification (objective lens magnification x eyepiece magnification) Numerical aperture 350 0.275 ➔ ± D.O.F. = N.A. × M + N.A.2 (λ=0.55µm) Objective n=1 (air) ω × 250,000 λ (µm) + N.A. × M 2(N.A.) 2 θ This indicates that the focal depth becomes smaller as the numerical aperture becomes larger. Sample surface Example With MPLFLN100×(N.A.=0.90), WHN10×: ± D.O.F. = 350 0.275 + = 0.39 + 0.34 = 0.73µm 0.90 × 1,000 0.81 7. Resolving Power ● Video camera In the case of a video camera, the focal depth will vary according to number of pixels of CCD, optical magnification, and numerical aperture. The above-mentioned formula is used as a rough guide only. The resolving power of an objective lens is measured by its ability to differentiate two lines or points in an object. The greater the resolving power, the smaller the minimum distance between two lines or points that can still be distinguished. The larger the N.A., the higher the resolving power. 44 OPTICAL TERMINOLOGY characteristics of glass materials used for the optical system. “Expansion of a point image” can also be expressed by “wavefront aberration” that regards the light as “waves” and takes account of the phase to include the influence of diffraction. 9. Aberrations A difference between an ideal image and an actual image that passes through an optical system is called an “aberration.” (1) Spherical aberration When light rays coming out of an axial object point enter a lens, the light rays with a larger numerical aperture (N.A.) are subjected to stronger refraction power and cross the optical axis in positions with larger differences from the ideal image formation position. The aberration caused this way by different image forming positions due to differences in N.A. of axial light rays is called “spherical aberration.” (“Spherical aberration” is proportional to the cube of N.A.) 9.1 Requirements for Ideal Image Formation The following three requirements must be satisfied to form an image with no aberration, or an ideal image. (i) All the light rays coming from a single point and passing through an image formation optical system converge on a single point. (ii) Image points, which correspond to object points on the same plane perpendicular to the optical axis, are present on the same plane. (iii) The planar shape of an object and the planar shape of an image that are on the same plane perpendicular to the optical axis have a similarity relation. Figure 9-1 Object Figure 9-3 Requirements for Ideal Image Formation (i) Image plane (ii) Specimen Spherical Aberration Aplanatic tube lens (iii) Objective lens with spherical aberration It is said that objective lenses with larger N.A. have better resolution but worsen spherical aberration. Our advanced design and manufacturing techniques have realized good optical performance even with large numerical aperture. In an actual optical system, however, it is very difficult to strictly meet the requirements for ideal image formation and this causes “aberrations” that interfere with image forming performance. (2) Coma aberration Even though spherical aberration is compensated to be very small, there are cases where light rays coming out of an off-axis object point are not condensed to a single point on the image plane but generate asymmetric blur just like a comet leaving traces. This is called coma aberration. 9.2 Classification of Aberrations Aberrations that interfere with image forming performance are classified as shown below in Figure 9-2. Seidel’s aberration = “Expansion of a point image” + “Curvature of image plane” + “Deformation” Figure 9-2 Image plane Figure 9-4 Classification of Aberrations Coma Aberration and Spot Shape on the Image Plane (1) Spherical aberration Specimen (2) Coma aberration Seidel's aberration Aplanatic tube lens (3) Astigmatism (4) Field curvature (5) Distortion Aberration Chromatic aberration Objective lens with coma aberration Image plane (6) Longitudinal (axial) chromatic aberration (7) Chromatic aberration of magnification (3) Astigmatism Even though a lens is compensated for spherical aberration and coma aberration, there are cases where an image of an off-axis object point is not focused to a single point but separated to a concentric line image and a radial line image. This is called “astigmatism.” When astigmatism is present, a point image blurs vertically and horizontally, before and after the focus position. Types (1) to (3) correspond to “expansion of a point image” that goes against requirement (i) for ideal image formation in Figure 91. Type (4) corresponds to “curvature of image plane” that goes against requirement (ii) in Figure 9-1. Type (5) corresponds to “deformation” that goes against requirement (iii) in Figure 9-1. Types (6) and (7) correspond to “color blur” of images caused by 45 OPTICAL TERMINOLOGY Figure 9-5 aberration of magnitude.” Many special glass materials are used, e.g., for apochromats (MPlanApo in Olympus), to eliminate chromatic aberration in a wide range from violet light (g-rays with wavelength of 435 nm) to red light (c-rays with wavelength of 656 nm). Astigmatism and Change in Spot Shape in Different Focus Positions 9.3 Wavefront Aberration Since a long time ago, aberrations have been used in “geometric optics,” which considers light as “light rays.” Microscope optical systems are often used for observation of very small specimens at a wavelength level, and sometimes adopt “wave optics,” which regards light as “waves” and handles the phase information, taking account of the influence of diffraction. In such a case, “wavefront aberration” is used for evaluation. As shown below, when requirements for ideal imaging are satisfied in a microscope optical system, the spherical wavefront (spherical waves) coming from a single point on an object (specimen) is converted to plane waves through an ideal objective lens. The plane waves are converted to spherical waves through an ideal tube lens, and condensed to a single point. The wavefront of these waves is called the “ideal wavefront.” (a) (b) (c) (a) (b) (c) (4) Field curvature An image plane of an object on a plane perpendicular to an optical axis does not always become a plane perpendicular to the optical axis, but it generally becomes a curved plane. This symptom is called “field curvature.” When field curvature is present, the image is more displaced as it becomes closer to the periphery of the visual field. Therefore, when the center of an image is brought into focus, blur occurs in the peripheral areas of the image. To bring the entire image, including the periphery, into clear focus, it is necessary to adequately compensate for this type of aberration. Figure 9-7 Specimen Ideal objective lens (5) Distortion When there is no similar relation between a planar shape on an object and a shape on the image plane, this is called “distortion.” When distortion is present, a square image appears in a shape of a barrel or pin-cushion as shown in Figure 9-6. Figure 9-6 Spherical wave Distortion (a) Barrel shape type Ideal Microscope Optical System Ideal tube lens Plane wave Image plane Spherical wave Based on the figure indicated for (1) spherical aberration, the behavior of the wavefront in an optical system that has an aberration is described below. (a) Pin-cushion type Figure 9-8 Illustration of Wavefront Aberration Actual wavefront Specimen The microscope optical system contains some distortion. When distortion is present, it can bring erroneous results of shape measurements. When a microscope is used for precision measurements, pay close attention to this aberration, for example, by providing it with an aberration compensation function. Ideal wavefront Objective lens with spherical aberration A difference (a degree of disagreement) between the ideal wavefront and the actual wavefront shown above is called “wavefront aberration.” (6) Chromatic aberration Glasses used for optical systems have different refractive indexes depending on the wavelength. This causes differences in focal length between wavelengths and generates displacement of image forming position. This phenomenon is called “chromatic aberration,” which is sometimes subdivided into axial displacement on the optical axis, called “axial chromatic aberration” (or lateral chromatic aberration) and displacement on the image plane, called “chromatic 46 OPTICAL TERMINOLOGY 9.4 Strehl ratio When a point light source is observed with an aberration-free optical system and an aberrated optical system , the former concentrates the focal point to a point at the image formation position. In contrast, the latter fails to produce a focal point, instead causing a spread in the intensity distribution of the point image (this is known as “point spread”). The specific appearance of such a point image (i.e. point spread) is shown in Fig. 9-9. Figure 9-9 Appearance of condensed light in the image plane (point spread) Aberration-free optical system Aberrated optical system With the proportion of light concentrated in the image plane (intensity of light concentrated in the Airy disk) by an aberrationfree optical system serving as 100%, the proportion of light concentrated by an aberrated optical system is known as the Strehl ratio. When graphed, the Strehl ratio reveals peaks in intensity as shown in Fig. 9-10. The higher the SR, the closer an optical system is to being aberration-free. Figure 9-10 Strehl ratio Intensity Aberration-free optical system 100% Aberrated optical system SR Image plane A Strehl ratio of 80% is typically called the diffraction limit, and lenses with a lower ratio lack the performance required to serve as an objective lens. A ratio of over 95% means that the lens’ performance in general observations is comparable to that of an aplanatic lens (which is corrected for spherical aberrations and coma). Note) A laser interferometer is used for actual assessment of optical performance, so assessment is done at a single wavelength. Unless otherwise noted, Strehl ratio measurements are at the e-line (544nm). 47 OLYMPUS CORPORATION has obtained ISO9001/ISO14001 Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Visit our Website for details. Specifications are subject to change without any obligation on the part of the manufacturer. Printed in Japan M1606E-0408B