Website http://www.eduKan.org Telephone 877-­‐433-­‐8526 (toll free) FAX -­‐ 620-­‐793-­‐5821 Postal address 1105 Main Street, Suite C, Great Bend, KS 67530 Instructor:
Pre-requisites:
Course Number/Title:
Credit Hours:
Required Text:
Course Specific Technology
(software, hardware, skills):
Jason Walker, M.S.
Please consult your college of enrollment Registrar.
BI280: Principles of Microbiology
5
There is no required text for this course. It contains an
embedded text, which you do not have to purchase separately.
Your tuition for this course will include the extra $115 textbook
fee.
You will receive, as part of your tuition the following software:
Wilson, G.R. 2004. Virtual Unknown: Microbiology. 3.11. Intuitive
Systems, Inc. Abilene, Texas. You should download this program
to a machine with a printer. You will use this program to
complete the on-line laboratory portion of the course. A PC and
Windows 98, ME, 2000, and XP will run this program. Other
requirements are: 64 MB RAM, 50 MB hard drive space, 800 x
600 resolution monitor, and and 2 MB video graphics card.
Please review the computer and browser requirements at:
http://edukan.org/user-login/technical/
Also since this course has the eText embedded, you may want to
view this document that explains how to manage your cookies
and pop-up blockers: Tech Doc
The student is responsible for having all necessary
equipment and software for this course. The instructor is
not responsible for hardware and software problems.
Technical difficulties with the course web page should be
reported to helpdesk@edukan.org, calling 1-866-3658326, or logging on to http://edukan.org/userlogin/technical/.
Technical problems associated with the laboratory
program should be reported to support@intuitiveinc.com.
Course Description - This course focuses on the study of microorganisms in relation to their physiology, morphology,
taxonomy, life cycles, and economic influences. Students will acquire skills in performing lab techniques involved in
culturing and studying microorganisms. The course is designed to meet the requirements of those interested in biology
and allied health sciences. Students will complete a lab in this course as part of the course requirements.
Learner Outcomes – The “learner outcomes” found in the table below are taken from the individual chapters in the
embedded eText found under Course Home. The table also shows which specific assessments (quizzes) address these
learner outcomes.
Information subject to change. Please check with one of our Academic Advisors at www.eduKan.org. Last update: 14 February 2014 Website http://www.eduKan.org Telephone 877-­‐433-­‐8526 (toll free) FAX -­‐ 620-­‐793-­‐5821 Postal address 1105 Main Street, Suite C, Great Bend, KS 67530 Learner Outcomes
Recognize the system of scientific nomenclature that uses two names: a
genus and a specific epithet.
Differentiate the major characteristics of each group of microorganisms.
Identify the contributions to microbiology made by Needham, Spallanzani,
Virchow, and Pasteur.
Explain the importance of observations made by Hooke and van
Leeuwenhoek.
Define bacteriology, mycology, parasitology, immunology, and virology.
Name two examples of biotechnology that use recombinant DNA technology
and two examples that do not.
Define biofilm.
Identify the importance of Koch's postulates.
Compare spontaneous generation and biogenesis.
List the three domains.
Define functional group.
Diagram three basic types of chemical reactions.
Define acid, base, salt, and pH.
Describe the role of ATP in cellular activities.
Define ionic bond, covalent bond, hydrogen bond, molecular weight,
Identify the building blocks of carbohydrates.
Identify the building blocks of nucleic acids.
Differentiate simple lipids, complex lipids, and steroids.
Identify a use for darkfield, phase-contrast, differential interference contrast,
fluorescence, confocal, two-photon, and scanning acoustic microscopy, and
compare each with brightfield illumination.
Explain why each of the following is used: capsule stain, endospore stain,
flagella stain.
Identify one use for the TEM, SEM, and scanned-probe microscopes.
Compare and contrast the Gram stain and the acid-fast stain.
List the steps in preparing a Gram stain, and describe the appearance of
gram-positive and gram-negative cells after each step.
Explain the purpose of simple staining.
Define total magnification and resolution.
Differentiate an acidic dye from a basic dye.
Diagram the path of light through a compound microscope.
Compare and contrast the cell walls of gram-positive bacteria, gram-negative
bacteria, acid-fast bacteria, archaea, and mycoplasmas.
Describe the functions of the nucleus, endoplasmic reticulum, Golgi complex,
lysosomes, vacuoles, mitochondria, chloroplasts, peroxisomes, and
centrosomes.
Differentiate flagella, axial filaments, fimbriae, and pilli.
Identify the functions of four inclusions.
Assessment(s)
Chapter 1 Quiz
Chapter 1 Quiz
Chapter 1 Quiz
Chapter 1 Quiz
Chapter 1 Quiz
Chapter 1 Quiz
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
1
1
1
1
2
2
2
2
2
2
2
2
3
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Chapter 3 Quiz
Chapter 3 Quiz
Chapter 3 Quiz
Chapter 3 Quiz
Chapter
Chapter
Chapter
Chapter
Chapter
3
3
3
3
4
Quiz
Quiz
Quiz
Quiz
Quiz
Chapter 4 Quiz
Chapter 4 Quiz
Chapter 4 Quiz
Information subject to change. Please check with one of our Academic Advisors at www.eduKan.org. Last update: 14 February 2014 Website http://www.eduKan.org Telephone 877-­‐433-­‐8526 (toll free) FAX -­‐ 620-­‐793-­‐5821 Postal address 1105 Main Street, Suite C, Great Bend, KS 67530 Compare and contrast the cell walls of gram-positive bacteria, gram-negative
bacteria, acid-fast bacteria, archaea, and mycoplasmas.
Define simple diffusion, facilitated diffusion, osmosis, active transport, and
group translocation.
Identify the functions of the nucleoid and ribosomes.
Describe the structure, chemistry, and functions of the prokaryotic plasma
membrane.
Compare and contrast the overall cell structure of prokaryotes and
eukaryotes.
Identify the three basic shapes of bacteria.
Describe the structure and function of the glycocalyx.
List and provide examples of three types of phosphorylation reactions that
generate ATP.
Compare and contrast aerobic and anaerobic respiration.
Describe how lipids and proteins undergo catabolism.
Identify the functions of the pentose phosphate and Entner- Doudoroff
pathways.
Distinguish competitive and noncompetitive inhibition.
Compare and contrast oxidative phosphorylation and photophosphorylation.
List the factors that influence enzymatic activity.
Categorize the various nutritional patterns among organisms according to
carbon source and mechanisms of carbohydrate catabolism and ATP
generation.
Explain the term oxidation-reduction.
Describe the patterns of microbial death caused by treatments with microbial
control agents.
Explain how the type of microbe affects the control of microbial growth.
Identify the methods of action and preferred uses of chemical disinfectants.
Compare the effectiveness of moist heat (boiling, autoclaving, pasteurization)
and dry heat.
Differentiate halogens used as antiseptics from halogens used as
disinfectants.
Distinguish chemically defined and complex media.
Explain four direct methods of measuring cell growth.
Compare the phases of microbial growth, and describe their relation to
generation time.
Classify microbes into five groups on the basis of preferred temperature
range.
Explain the importance of osmotic pressure to microbial growth.
Identify ways in which aerobes avoid damage by toxic forms of oxygen.
Explain three indirect methods of measuring cell growth.
Explain the regulation of gene expression in bacteria by induction, repression,
and catabolite repression.
Compare the mechanisms of genetic recombination in bacteria.
Differentiate horizontal and vertical gene transfer.
Chapter 4 Quiz
Chapter 4 Quiz
Chapter 4 Quiz
Chapter 4 Quiz
Chapter 4 Quiz
Chapter 4 Quiz
Chapter 4 Quiz
Chapter 5 Quiz
Chapter 5 Quiz
Chapter 5 Quiz
Chapter 5 Quiz
Chapter
Chapter
Chapter
Chapter
5
5
5
5
Quiz
Quiz
Quiz
Quiz
Chapter 5 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapters 6 and 7 Quiz
Chapter 8 Quiz
Chapter 8 Quiz
Chapter 8 Quiz
Information subject to change. Please check with one of our Academic Advisors at www.eduKan.org. Last update: 14 February 2014 Website http://www.eduKan.org Telephone 877-­‐433-­‐8526 (toll free) FAX -­‐ 620-­‐793-­‐5821 Postal address 1105 Main Street, Suite C, Great Bend, KS 67530 Describe the process of DNA replication.
Describe the functions of plasmids and transposons.
Describe protein synthesis, including transcription, RNA processing, and
translation.
Describe how DNA serves as genetic information.
Describe the effect of mutagens on the mutation rate.
Define genetics, genome, chromosome, gene, genetic code, genotype,
phenotype, and genomics.
Compare commensalism, mutualism, and parasitism, and give an example of
each.
Categorize diseases according to severity.
Categorize diseases according to frequency of occurrence.
Define epidemiology, and describe three types of epidemiologic
investigations.
Explain three methods of disease transmission.
Define normal and transient microbiota.
List several probable reasons for emerging infectious diseases, and name one
example for each reason.
List Koch's postulates.
Differentiate a communicable from a noncommunicable disease.
Explain how nosocomial infections can be prevented.
Define nosocomial infections, and explain their importance.
Define reservoir of infection.
Define epidemiology, and describe three types of epidemiologic
investigations.
Outline the mechanisms of action of A-B toxins, membrane-disrupting toxins,
and superantigens. Classify diphtheria toxin, erythrogenic toxin, botulinum
toxin, tetanus toxin, Vibrio enterotoxin, and staphylococcal enterotoxin.
Explain how capsules and cell wall components contribute to pathogenicity.
Using examples, describe the roles of plasmids and lysogeny in pathogenicity.
Contrast the nature and effects of exotoxins and endotoxins.
Using examples, explain how microbes adhere to host cells.
Discuss the causes of symptoms in fungal, protozoan, helminthic, and algal
diseases.
Identify the principal portals of entry.
Discuss the causes of symptoms in fungal, protozoan, helminthic, and algal
diseases.
Define ID50 and LD50.
Differentiate TH1 and TH2 cells.
Distinguish a primary from a secondary immune response.
Differentiate T helper, T cytotoxic, and T regulatory cells.
Describe the function of natural killer cells.
Describe at least one function of each of the following: M cells, TH1 cells, TH2
cells, TC cells, Treg cells, CTLs, NK cells.
Chapter 8 Quiz
Chapter 8 Quiz
Chapter 8 Quiz
Chapter 8 Quiz
Chapter 8 Quiz
Chapter 8 Quiz
Chapter 14 Quiz
Chapter 14 Quiz
Chapter 14 Quiz
Chapter 14 Quiz
Chapter 14 Quiz
Chapter 14 Quiz
Chapter 14 Quiz
Chapter
Chapter
Chapter
Chapter
Chapter
Chapter
14
14
14
14
14
14
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Chapter 15 Quiz
Chapter
Chapter
Chapter
Chapter
Chapter
15
15
15
15
15
Quiz
Quiz
Quiz
Quiz
Quiz
Chapter 15 Quiz
Chapter 15 Quiz
Chapter 15 Quiz
Chapters 16 and
Chapters 16 and
Chapters 16 and
Chapters 16 and
Chapters 16 and
17
17
17
17
17
Quiz
Quiz
Quiz
Quiz
Quiz
Information subject to change. Please check with one of our Academic Advisors at www.eduKan.org. Last update: 14 February 2014 Website http://www.eduKan.org Telephone 877-­‐433-­‐8526 (toll free) FAX -­‐ 620-­‐793-­‐5821 Postal address 1105 Main Street, Suite C, Great Bend, KS 67530 Compare and contrast T-dependent and T-independent antigens.
Contrast the four types of adaptive immunity.
Describe the cause and effects of fever.
Classify leukocytes, and describe the roles of granulocytes and monocytes.
Compare and contrast the actions of IFN-? and IFN-? with IFN-?.
Define Toll-like receptors.
Describe three consequences of complement activation.
Describe the role of iron-binding proteins in innate immunity.
Identify six mechanisms of avoiding destruction by phagocytosis.
Chapters
Chapters
Chapters
Chapters
Chapters
Chapters
Chapters
Chapters
Chapters
16
16
16
16
16
16
16
16
16
and
and
and
and
and
and
and
and
and
17
17
17
17
17
17
17
17
17
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
Quiz
The complete list of Core Outcomes and Competencies may be viewed at:
http://kansasregents.org/resources/PDF/1812-2011-12KansasCoreOutcomesReport01312012.pdf, pages 21-22
The learning outcomes and competencies detailed in this syllabus meet, or exceed the learning
outcomes and competencies specified by the Kansas Core Outcomes Project for this course, as
sanctioned by the Kansas Board of Regents.
Methods of Instruction - This course is web-based, and does NOT include a hands-on laboratory component. The
method of instruction will include, but is not necessarily limited to: Power Point presentations, chapter outlines, reading
assignments, videos, tutorials/activities, threaded discussions, virtual laboratory exercises, case studies, quizzes and
examinations. The ‘outlines’ within each unit cover the chapter for that particular unit. These outlines should not be
viewed as a complete list of information necessary for the exam. These outlines are intended to serve as study guides.
Methods of Assessment – The course will be divided into 10 units. Each unit will have an outline of the text material,
virtual laboratory (20 points), threaded discussion (20 points), and quiz (40 points). In addition, there will be two exams
(100 points each). The first exam is located within Unit 5 and is due 3 days after the end of the Unit 5 period. The
second exam is within Unit 10 and is due by three days after the end of the Unit 10 period. Final grades will be
determined based the following scale: 90-100% = A, 80-89% = B, 70-79% = C, 60-69% = D, 59% and below = F.
ALL ASSIGNMENTS ARE DUE BY MIDNIGHT ON THE DUE DATE AS OUTLINED BY THIS SYLLABUS.
Course Requirements –
Review Questions (homework):
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•
For each unit, students will be assigned selected chapter readings.
Each set of review questions will consist of 40 MC and/or T/F questions pertaining to these chapters.
Unlimited access is granted to these questions. You will not be able to take the entire assignment again, but you
will be able to take each individual Review Question as many times as it takes before go on. This activity will
close on the unit due date posted in this syllabus.
These are there for you to practice and a grade will not be taken on them.
Virtual Laboratory Exercises: Students are required to complete several virtual laboratory exercises for this course.
Information subject to change. Please check with one of our Academic Advisors at www.eduKan.org. Last update: 14 February 2014 Website http://www.eduKan.org Telephone 877-­‐433-­‐8526 (toll free) FAX -­‐ 620-­‐793-­‐5821 Postal address 1105 Main Street, Suite C, Great Bend, KS 67530 •
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•
•
•
These activities will be taken from Virtual Unknown Microbiology. Virtual Unknown Microbiology is a lab simulation
that assigns students an unknown microbe at the beginning of each activity. Over 50 microbiological tests
including Gram staining, carbohydrate tests, nitrite metabolism, blood hemolysis, etc. are included to help identify
124 bacterial species. These activities are highly realistic and will utilize the basic procedures learned in the
hands-on laboratory sessions.
You will be required to click on the "Virtual Lab" link under each unit, use the VUMIE program to answer the 10
questions found there, and then submit BOTH the questions AND the answers in a Journal entry. You need to
label the Journal entry according to the lab that you are doing, i.e. Unit 1 Lab or Lab 1.
Each unit’s virtual lab activity will be worth 20 points.
Virtual lab assignments are to be entered in your journal. Journals can be accessed by clicking on the "Journal"
tab at the top of the screen.
Journal entries must be titled as specified for each assignment. It is easiest to compose your lab report as a
separate Word document, then copy and paste to your journal.
Threaded Discussions: Threaded discussions are designed to encourage discussion, cooperation and teamwork.
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The discussions will be found under each unit.
Each unit’s discussion will either be centered on the unit’s virtual lab activity or the content found in the
chapter(s) in that unit.
The instructor will make an initial thread to which every student must respond. In addition, each student must
respond to at least two additional peer threads. Points will not be given to entries that are not contributory to the
"discussion", i.e. responses that takes the peer's post and goes more in-depth OR gives an explanation of why
the student disagrees with the reasoning of the original post. For example, stating "Good post, Jason...I agree!"
will not be given any points.
The discussion is worth 20 points (12 for the original entry, 4 for each peer response).
Discussions will not be allowed to be turned in late. If you neglect to participate in the unit's discussion, then you
will be given a zero for that discussion.
Quizzes: Each unit will end with a short quiz.
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•
•
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•
The quizzes can be found under the Mastering Assignments link under Course Home.
These quizzes will include 20 questions to test the student’s knowledge of the unit material.
Students will be allowed 20 minutes to complete each quiz. Please make sure that you study the review
questions and other materials found in the "Unit Game Plan" in each unit before attempting the quiz!
Each quiz is worth 40 points.
You are not allowed to use your notes, other people, textbook material, or any other aid when taking a quiz, i.e.
you are not allowed to use anything but your brain on the quizzes.
Exams: You will have two cumulative exams in this course.
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The exams can be found under the Mastering Assignments link under Course Home.
The first exam will cover the first half of the units (1 – 5).
The second exam will cover the second half of the units (6 – 10).
The exams will consist of multiple-choice and true/false questions (50 in all).
Students will be allowed 60 minutes to complete each exam.
Information subject to change. Please check with one of our Academic Advisors at www.eduKan.org. Last update: 14 February 2014 Website http://www.eduKan.org Telephone 877-­‐433-­‐8526 (toll free) FAX -­‐ 620-­‐793-­‐5821 Postal address 1105 Main Street, Suite C, Great Bend, KS 67530 •
•
Students will be able to access the exam only one time.
Each exam is worth 100 points. For security reasons, students will NOT be allowed to view the exam after
completion.
Course Outline –
Microbial cell biology
• Structure and function of prokaryotic and eukaryotic organisms
• Structure and function of acellular infectious agents
• Growth and division
• Energy metabolism
• Regulation of cellular activities
Microbial genetics
• Inheritance and flow of information
• Causes, consequences, and significance of mutations
• Exchange and acquisition of genetic information
• Genetic engineering
• Biotechnology
Interactions of microorganisms and humans
• Host defense mechanisms and immune systems
• Pathogenicity mechanisms of cellular and acellular infectious agents
• Disease transmission
• Control of microorganisms
• Antimicrobial agents
• Epidemiology and public health
• Adaptation and natural selection
• Symbiosis
Interactions and impact of microorganisms in the environment
• Microbial recycling of resources
• Microbes transforming the environment
Assignment Policy – Students are expected to complete and turn in all assignments and exams by the due date
established by the instructor. All due dates will be posted in the syllabus by the first day of class. All late assignments will
be given 1/2 credit up up to one week late. After this time the assignment will be given a zero. Please review the due
dates on the calendar directly above this statement. All due dates are on Sundays. It is your responsibility to budget
your time and get your assignments complete by the due date as seen above.
Expectations of instructor for successful completion of the course:
Students are expected to have all necessary equipment, books, and software prior to the beginning of the class. Students
are expected to complete all course material by the due dates specified in the course outline below. Students are
expected to study appropriately for successful completion of quizzes and exams. Students are expected to participate in
the threaded discussions and laboratory activities. You are not allowed to use your notes, other people, textbook
material, or any other aid when taking an exam, i.e. you are not allowed to use anything but your brain on the exams.
Information subject to change. Please check with one of our Academic Advisors at www.eduKan.org. Last update: 14 February 2014 Website http://www.eduKan.org Telephone 877-­‐433-­‐8526 (toll free) FAX -­‐ 620-­‐793-­‐5821 Postal address 1105 Main Street, Suite C, Great Bend, KS 67530 eduKan Student Verification Policy – eduKan requires all eduKan students to complete the BioSig ID Registration and
at least one successful validation during the first week of each session. A minimum of six validations should be
completed prior to the first “gated” exam in each course. Students who refuse to complete a BioSig ID registration and
validation by the course mid-term may be required to have the final exam, quiz, or other assessment proctored.
If the student is required to proctor and fails to complete the paperwork prior to the scheduled assessment,
does not show up for the scheduled assessment or refuses to take the final, a failing grade (F) will be
assigned for the course.
eduKan Proctored Assessment Policy – The instructor has the option to randomly select or designate
students to take a proctored assessment at any time during the course. If the student is required to proctor
and fails to complete the paperwork prior to the scheduled assessment, does not show up for the scheduled
assessment or refuses to take the final, a failing grade (F) will be assigned for the course.
Interaction and Participation Policy –
Participation Policy: Students are expected to complete all assignments and exams by the due date established by the
instructor. Participation will be evaluated based on your successful completion of threaded discussions, assignments and
exams.
Email Policy: Each email correspondence with the instructor MUST include the course title (EduKan Microbiology) in the
subject box, the student’s full name, and the exact item of concern. In addition, please use language suitable for a
professional setting.
FIRST WEEK OF CLASS: Please read the syllabus carefully. The syllabus is found under the Course Home bullet that you
see to your left. Within the first two days of class, complete the Course Home discussion and syllabus quiz. These
activities are worth 10 points each. You are also required within the first week to register and validate using BioSig. To
do this click on the BioSig ID Registration/Validation link under Course Home and follow the directions. All three of these
assignments need to be completed the first week of class and will be used to determine if you are "present" or not in the
course. Those choosing not to complete these by the drop deadline run the risk of being dropped, therefore please get
these completed the very first week of the course!
The most important thing you need to remember is that I want nothing less than to help you be as successful as you
possibly can in this course. In each unit, I have placed a discussion board called "Questions for the Prof". Please do not
ever hesitate to let me know what questions you have, whether it is in regards to content, the lab, or any other aspect of
this course. Use this discussion board to ask your questions so that your classmates and I can help you the best way
possible. If you don't want to put your question/comment on the "Questions for the Prof" discussion, just email me and I
will get back to you as soon as possible.
Information subject to change. Please check with one of our Academic Advisors at www.eduKan.org. Last update: 14 February 2014