Thermo Scientific
GeneJET Nucleic
Acid Purification Kits
high yields of pure DNA & RNA
from different sources
High Yields • Exceptional Purity • Incredible Value
Reliable DNA and RNA Purification
The Thermo Scientific GeneJET product line contains
essential nucleic acid purification tools to support
and facilitate your experimental workflow. With
more than 30 years of experience in developing and
manufacturing molecular biology products, we see
a growing demand for simple and reliable tools for
DNA and RNA sample preparation. The GeneJETTM
DNA and RNA purification kits address the need for
high-purity nucleic acids ready to use in downstream
applications and offer detailed protocols for routine
and difficult sample types.
• Trusted silica membrane technology in
the form of a convenient spin column for
purification of DNA and RNA
• Isolate nucleic acids with superior purity and
yields compared to other kits on the market
• Purified nucleic acids are ready to use in
common downstream applications
• GeneJET kits have been extensively tested
on a wide variety of sample types and include
specialized protocols
Product Selection Guide
Nucleic Acid
Purification
Plasmid
DNA
Miniprep
Midiprep
for details see
page 3
Genomic
DNA
Maxiprep
Plant
DNA
Blood
DNA
for details see
page 4
DNA
Fragments
Total RNA
DNA from
Cells &
Tissues
Plant
RNA
Blood
RNA
RNA from
Cells &
Tissues
for details see
page 5
From PCR
From Gels
for details see
page 6
For additional details visit www.thermoscientific.com/genejet
GeneJET Genomic DNA Purification Kits
Efficient genomic DNA purification from different sources.
GeneJET Genomic DNA Purification Kit
• Purify RNA-free gDNA from a wide variety of tissues or cells in only
20 minutes
GeneJET Whole Blood Genomic DNA Purification Mini Kit
• Rapidly purify RNA-free gDNA from whole blood and body fluids
with no separate cell lysis step
GeneJET Plant Genomic DNA Purification Mini Kit
• Purify DNA without clogging-prone lysate filtration step
• Material-specific protocols provided
• Recover gDNA greater than 30 kb in size, including nuclear,
mitochondrial and chloroplast DNA
Isolate genomic DNA
from a variety of samples:
• Mammalian cells
• Mammalian tissues:
• Muscle
• Heart
• Liver
• Whole blood
• Fresh or frozen
Spin-columns based on silica membrane technology
• Anticoagulated blood
Isolated DNA can be used directly in PCR, qPCR, and other
common enzymatic reactions
• Buffy coat
• Bone marrow
• Body fluids (urine)
Specifications for GeneJET Genomic DNA Purification Kits
Genomic DNA Purification
• Plants
• Multiple plant species
Cells & tissues
Whole Blood
Plant
Amount of sample material
Varies
200 µl
100 mg
Yield
Varies
Up to 10 µg
Up to 32 µg
Purity A260/280
1.7-1.9
1.7-1.9
1.7-1.9
• Yeasts
Column binding capacity
≥50 µg
≥50 µg
≥50 µg
• Filamentous fungi
Procedural time
20 min
20 min
30 min
• Bacteria
Spin columns, capped, preassembled with collection tubes
and individually packed. Centrifugation or vacuum protocol.
Format and protocol
High yields of genomic DNA purified from various sources with the
GeneJET Genomic DNA Purification Kit
30
Genomic DNA, µg
• Various plant tissues (leaves, roots, seed, needles)
Plant genomic DNA purified from various
sources with the GeneJET Plant Genomic
DNA Purification Mini Kit
Thermo Scientific
vendor A
vendor B
vendor C
25
20
15
10
5
0
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
rat liver
10 mg
rat spleen
5 mg
mouse heart
10 mg
HeLa
2x108 cells
E. coli mammalian blood
2x109 cells
200 µl
Yields of genomic DNA purified using the GeneJET Genomic DNA Purification Kit and kits
from other vendors.
Product
Catalog #
Preps
GeneJET Genomic DNA Purification Kit
K0721
K0722
50
250
GeneJET Whole Blood Genomic DNA
Purification Mini Kit
K0781
K0782
50
250
GeneJET Plant Genomic DNA Purification
Mini Kit
K0791
K0792
50
250
Genomic DNA from different plant material
samples purified using the GeneJET Plant
Genomic DNA Purification Mini Kit. 1- Arabidopsis,
2- Tobacco leaves, 3- Tomato leaves, 4- Spinach
leaves, 5- Wheat leaves, 6- Grape leaves, 7- Onion
leaves, 8- Rice leaves, 9- Corn seeds, 10- Sunflower
stalk, 11- Corn roots, 12- Beef roots, 13- Cucumber
fruit, 14 - Sugar cane.
GeneJET Plasmid DNA Purification Kits
Rapid isolation of microgram to milligram quantities of highquality plasmid DNA.
GeneJET Plasmid Miniprep Kit
•Small scale isolation of up to 20 µg of high
quality plasmid DNA in 14 min
Consistent and Reproducible Miniprep Yields
Relaxed
Supercoiled
GeneJET Plasmid Midiprep Kit
•Medium scale isolation of up to 200 µg of
high quality plasmid DNA in 60 min
GeneJET Plasmid Maxiprep Kit
•Large scale isolation of up to 750 µg of
high quality plasmid DNA in 60 min
M
1
2
3
4
5
6
A pBluescript-based high copy number
plasmid was purified from E.coli culture
with 6 different GeneJET Spin Columns
Silica-based membrane technology - save
time and improve yields compared to
gravity flow columns.
M - FastRuler High Range DNA Ladder
1-6 - plasmid DNA isolated with 6 different GeneJET Spin Columns
Isolated plasmid DNA can be used directly
in FastDigest or conventional restriction digestion, sequencing, PCR, in vitro
transcription and transfection of robust cell lines.
Specifications for GeneJET Plasmid DNA Purification Kits
Plasmid Purification Kits
Bacterial culture volume
Yield
Mini
Midi
Maxi
5 ml
50 ml
250 ml
20 µg
200 µg
750 µg
≥400 ng/µl
≥400 ng/µl
≥400 ng/µl
1.75-1.9
1.75-1.9
1.75-1.9
Column binding capacity
20 µg
400 µg
1000 µg
Procedural time
14 min
60 min
60 min
Format and protocol types
Spin columns, capped, preassembled with collection tubes.
Centrifugation or vacuum protocol.
DNA concentration
Purity A260/280
High yields and high concentration plasmid DNA purified with the GeneJET Midiprep Kit.
250
1st elution
2nd elution
DNA, ng/µg
DNA, µg
200
150
100
50
0
Thermo
Scientific
vendor
A
vendor
B
vendor
C
vendor
D
Yields of high–copy plasmid DNA obtained using
GeneJET plasmid midiprep kit and other kits for
midscale plasmid DNA purification at maximal
recommended bacterial cell culture volumes.
Product
900
800
700
600
500
400
300
200
100
0
1st elution
2nd elution
Thermo
Scientific
vendor
A
vendor
B
vendor
C
vendor
D
Concentrations of eluted DNA obtained after the
purification of high–copy plasmid DNA using different
kits for midscale plasmid DNA purification at maximal
recommended bacterial cell culture volumes.
Catalog #
Preps
GeneJET Plasmid DNA Miniprep Kit
K0502
K0503
50
250
GeneJET Plasmid DNA Midiprep Kit
K0481
K0482
25
100
GeneJET Plasmid DNA Maxiprep Kit
K0491
K0492
10
25
Midi and Maxi
spin columns offer
considerably faster
procedure and higher
yields compared to
gravity flow anion
exchange columns
GeneJET Total RNA Purification Kits
High purity total RNA isolation from different sources.
Isolate RNA from a
variety of samples:
• Mammalian cells
• Mammalian tissues:
• Muscle
• Heart
• Liver
• Whole blood
• Anticoagulated blood
GeneJET RNA Purification Kit
• Purify total RNA from a wide variety of sources
GeneJET Whole Blood RNA Purification Mini Kit
• Purify RNA from whole blood and body fluids with no DNase step
GeneJET Plant RNA Purification Mini Kit
• Purify RNA from various plants and plant tissues with no DNase step
Spin-columns based on silica membrane technology
Specialized and detailed protocols for different sample types
Purified RNA can be used in a wide range of downstream applications
• Buffy coat
Specifications for GeneJET RNA Purification Kits
• Bone marrow
RNA Purification Kits
Cells & tissues
Whole Blood
Plants
• Multiple plant species
Amount of sample material
Varies
500 µl
50 mg
• Various plant tissues (leaves,
roots, seed, needles)
Yield
Up to 70 µg
Up to 5 µg
Up to 70 µg
Purity A260/280
1.9-2.1
1.9-2.1
1.9-2.1
• Bacteria
Column binding capacity
>100 µg
>100 µg
>100 µg
• Yeast
Procedural time
15 min
15 min
20 min
• Plants
• Pathogens (virus)
• Filamentous fungi
Spin columns, capped, preassembled with collection tubes and
individually packed. Centrifugation protocol.
Format and protocol types
Total RNA purified from various sources with the GeneJET RNA Purification Kits.
M
1
2
3
4
5
Total RNA purified from 50 mg of tomato
leaves using the GeneJET Plant RNA
Purification Kit and kits from other vendors.
M- RiboRuler High Range RNA Ladder.
1- Thermo Scientific, 2-5 other vendors.
Product
M
1
2
3
4
5
6
Total RNA purified from various sources using the
GeneJET RNA Purification Kit.
M- RiboRuler™ High Range RNA Ladder (#SM1821)
1- mouse liver, 2- mouse spleen, 3- HeLa cells,
4- Cos-7 cells, 5- Saccharomyces cerevisiae,
6- Escherichia coli DH5α
Catalog #
Preps
GeneJET RNA Purification Kit
K0731
K0732
50
250
GeneJET Whole Blood RNA Purification Mini Kit
K0761
50
GeneJET Plant RNA Purification Mini Kit
K0801
K0802
50
250
GeneJET PCR Purification and Gel
Extraction Kits
Effective DNA fragment recovery from reaction
mixtures or agarose gels.
GeneJET Gel Extraction Kit
• DNA recovery up to 95% (25 bp – 20 kb)
• Purify DNA fragments from standard or low-melt agarose
gels run with TAE or TBE buffer.
Superior purification of DNA fragments from PCR
reaction mixtures
100
Recovery rate, %
GeneJET PCR Purification Kit
• DNA recovery up to 100% (25 bp – 20 kb)
• Effective removal of primers, unincorporated nucleotides,
enzymes and salts from PCR and other enzymatic
reaction mixtures
Thermo Scientific
vendor A
vendor B
80
60
40
20
0
100 bp
500 bp
1 kb
Size
Spin-columns based on silica membrane technology
DNA can be used in common downstream applications such as sequencing,
restriction digestion, ligation, cloning and in vitro transcription
Specifications for GeneJET
PCR Purification and Gel
Extraction Kits Kit types
PCR Purification
Gel Extraction
0.8 ml
1 g agarose gel
90-100%
70-95%
25 bp-20 kb
25 bp-20 kb
1.7-1.9
1.7-1.9
Column binding capacity
25 µg
25 µg
Procedural time
5 min
15 min
Amount of sample material
Recovery rate
DNA fragment range
Purity A260/280
Format and protocol types
Product
Spin columns, capped, preassembled with collection tubes.
Centrifugation or vacuum protocol.
Catalog #
Preps
GeneJET PCR Purification Kit
K0701
K0702
50
250
GeneJET Gel Extraction Kit
K0691
K0692
50
250
For further information visit www.thermoscientific.com/genejet
Ordering Information:
North America www.thermoscientific.com/fermentas
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Lafayette, CO 80026
Toll free: 800-340-9026
Fax: 800-472-8322
Europe
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Tel: +49 (0) 6227 35 67 90
Fax: +49 (0) 6227 5 36 94
© 2011 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
US Literature # 0012411J01U
Euro Literature # PB_2011_186
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