NAME:
Virtual Fly Lab:
PARTNER NAME
(Adapted from Richard Borinsky and Kim Foglia)
Use the procedure sheet to help guide you how to use the virtual fly lab. You must show YOUR PUNNET SQUARES
FOR ALL PROBLEMS RECORD CHISQUARE RESULTS.
Which of the following applies to the problems in this exercise? Choose the appropriate
response and place it in the portion of the question labeled "Final Analysis".
a. Monohybrid inheritance. Autosomal recessive
b. Monohybrid inheritance. Autosomal dominant
c. Monohybrid inheritance. X-linked dominant
d. Monohybrid inheritance. X-linked recessive
e. Dihybrid inheritance. No linkage. Both autosomal recessive
f.
Dihybrid inheritance. Both recessive. One is X-linked and one is autosomal.
g. Two linked autosomal recessives. How many map units separate the linked genes?
h. Three nonlinked autosomal recessives.
i.
Three linked autosomal recessives. How many map units separate each of the alleles? Draw a
map showing the order of the alleles on the chromosome.
Practice Problem #1 ------------Vestigial x Wild
Phenotype - symbols
Number
Proportion
Ratio
CHI-SQUARED ANALYSIS OF RESULTS
Phenotype Symbols
Observed
Hypothesis
Expected
Chi Square
Term
F2 Female:
F2 Male:
Chi square test statistic = _______________
Degrees of freedom = __________________
Level of Significance = _________________
Recommendation:
Reject
Do not reject
Final Analysis: _______________
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Practice Problem #2 ------------Shaven Bristled x Wild
Phenotype - symbols
Number
Proportion
Ratio
Number
Proportion
Ratio
Final Analysis: _____________
Assignment Problem #1------------Brown eyes x Wild
Phenotype - symbols
:
CHI-SQUARED ANALYSIS OF RESULTS
Phenotype Symbols
Observed
Hypothesis
Expected
Chi Square
Term
F2 Female:
F2 Male:
Chi square test statistic = _______________
Degrees of freedom = __________________
Level of Significance = _________________
Recommendation:
Reject
Do not reject
Final Analysis: __________
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Assignment Problem #2------------Incomplete wing vein/purple eyes x Wild wings/wild eyes
Phenotype - symbols
Number
Proportion
Ratio
CHI-SQUARED ANALYSIS OF RESULTS
Phenotype Symbols
Observed
Hypothesis
Expected
Chi Square
Term
F2 Female:
F2 Male:
Chi square test statistic = _______________
Degrees of freedom = __________________
Level of Significance = _________________
Recommendation:
Reject
Do not reject
Final Analysis: ___________
Assignment Problem #3------------Apterous wings/sepia eyes x wild wings/wild eyes
Phenotype - symbols
Number
Proportion
Final Analysis: __________
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Ratio
Assignment Problem #4------------white eyes x Wild eyes
Phenotype - symbols
Number
Proportion
Ratio
Proportion
Ratio
Number
Proportion
Ratio
Number
Proportion
Ratio
Final Analysis: _________
Assignment Problem #5------------yellow body x Wild body
Phenotype - symbols
Number
Final Analysis: _________
Assignment Problem #6------------crossveinless-winged x Wild
Run test cross: homozygous recessive female and heterozygous male
Phenotype - symbols
Final Analysis: _________
Assignment Problem #7------------Barr eyes x Wild eyes
Phenotype - symbols
Final Analysis: _________
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Assignment Problem #8------------black body/sepia eyes x Wild body and wild eyes
Phenotype - symbols
Number
Proportion
Ratio
Final Analysis: ______________
Assignment Problem #9-------Spineless /ebony /incomplete wing vein x Wild bristles / wild body /wild wing veins
Final Analysis: _______________
Determine if the alleles for the 3 traits are linked by doing the following test crosses:
1. spineless, ebony, wild-----------------
linked
unlinked
2. ebony, incomplete, wild
linked
unlinked
3. spineless, incomplete and wild
linked
unlinked
If they are linked, how many map units apart are
1. spineless and ebony_______________
2. spineless and incomplete____________________
3. ebony and incomplete_______________________
What is the order of the 3 genes?
a. SEI
B. ESI
C. SIE
Draw the alleles on the line below showing the distances between each allele.
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Assessment Exercise: Virtual Fly
For this part of your assignment, you will use the Virtual Fly Website as before. You and a partner or two will
assigned a group of four traits(recessive, dominant, X-link, lethal, epistasis…)
You will need to develop Punnet Squares as hypotheses BEFORE you complete crosses and CHI SQUARE
analysis. A note: Parental flies in FlyLab are true breeding homozygotes. THE EXCEPTION IS IF A TRAIT IS
A LETHAL DOMINANT. It is only lethal in the HOMOZYGOTE (AA). Therefore, when you set up the cross
with a Lethal dominant trait, the FlyLab software automatically makes the breeding fly with the trait a
HETEROZYGOTE (Aa), because if the fly was homozygous, the fly would be dead. ---therefore the
heterzygote is the only way to get the trait into a breeding pair.
Number
1
Student Names
Trait 1
AP
Trait 2
L
Trait 3
Y
Trait 4
AR
2
BL
VG
W
CY
3
BW
L
T
D
4
C
AP
SN
SB
5
DP
VG
SD
ST
6
E
SS
S
ST
7
PR
L
M
SB
8
RI
VG
F
D
9
BW
AP
CV
CY
10
SS
L
Y
AR
11
SV
EY
B
AR
12
VG
L
W
CY
13
AP
L
T
D
14
EY
BW
SN
SB
15
VG
L
SD
ST
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DROSOPHILA MUTATIONS
ABBREVIATION
MUTANT PHENOTYPE
ABBREVIATION
MUTATION PHENOTYPE
AP
AR
B
BL
BW
C
CV
CY
D
E
EY
DP
F
L
APTEROUS WINGS
ARITAPEDIA ANTENNAE
BAR EYES
BLACK BODY
BROWN EYES
CURVED WINGS
CROSSEINLESS WINGS
CURLY WINGS
DICHAETE WINGS
EBONY BODY
EYELESS EYES
DUMPY WINGS
FORKED BRISTLES
LOBE EYES
M
PR
RI
S
SB
SD
SE
SN
SS
ST
SV
T
VG
W
Y
MINIATURE WINGS
PURPLE EYES
RADIUS IMCOMPLETUS WINGS
SABLE BODY
STUBBLE BRISTLE
SCALLOPED WINGS
SEPIA EYES
SINGED BRISTLES
SPINELESS BRISTLES
STAR EYES
SHAVEN BRISTLES
TAN BODY
VESTIGIAL WINGS
WHITE EYES
YELLOW BODY
For each of your assigned traits, complete the following chart to summarize your findings on
the genetic mechanisms that control their inheritance.
Abbreviation
Mutation Phenotype
DOM/REC
X-LINKED
LETHAL
EPISTATIC
For your investigation of multiple gene interactions (test for linkage…), complete the following
charts to summarize your findings.
Abbreviations
Mutation Phenotypes
Linkage
Cross-over frequency
Questions:
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Map Units (M.U.)
1. Are any of the traits on the same chromosome? _______________________
2. If yes, which traits are linked? ______________________________________
3. How do you know they are linked?
_________________________________________________________________________________________________
___________
_________________________________________________________________________________________________
___________
4. Determine the cross over frequency and determine the map units:
_________________________________________________________________________________________________
___________
_________________________________________________________________________________________________
___________
5. Which traits are independent? _____________________________________________
6. How do you know they are independent?
_________________________________________________________________________________________________
___________
_________________________________________________________________________________________________
___________
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