6th Grade SAFETY ADVANCED PREPARATION Thinking Like a 21st Century Scientist / Engineer: Laboratory Procedures and Equipment Students should follow all lab safety procedures and rules for using glassware and liquids. If a test tube is broken, do not let students clean up the broken glass. The teacher should dispose of broken glass appropriately by placing in a secure receptacle for proper disposal. Note: Even though students are using water colored with food coloring, it is important for the students to complete this lab as if they were using chemicals in order to reinforce proper lab safety practices. (e.g. wearing safety glasses, aprons) Prepare 3 containers of colored water using food coloring (red,yellow,blue) Gather supplies for Rainbow Lab. Each group will need: -test tube rack containing 6 test tubes (for holding liquids)…label A-F -3 small beakers (for RED, YELLOW, and BLUE liquids) -1 small graduated cylinder (for measuring) -1 large graduated cylinder (for measuring) -1 bottle of water and large beaker (for rinsing) -1 eye dropper/pipette (for transferring liquids) -apron -safety glasses - lab tray Objective: Students will learn about proper laboratory procedures and equipment, as well as practice following lab safety guidelines through a SpongeBob reinforcement worksheet and by completing a laboratory activity involving measuring and mixing colored liquids. ACTIVITIES (2 days) What is the teacher doing? What are the students doing? Lab Safety Reinforcement and Rainbow Lab Pre-lab (Day 1) Lab Safety Reinforcement and Rainbow Lab Pre-lab (Day 1) Distribute SpongeBob Science WS. This lesson is from http://www.sciencespot.net/ Media/scimthdsafety.pdf Distribute the Rainbow Lab Pre-Lab. Discuss lab goals and assist students as they compete the pre-lab. Columbus City Schools Curriculum Leadership and Development Science Office June 2013 1. Students read the SpongeBob Experiment Scenario. Circle all of the lab/safety tools that SpongeBob, Patrick, and Gary use. List 10 safety rules that they break. 2. Students complete the pre-lab by reading about the experiment and determining the equipment needed to complete the experiment. They will also identify lab expectations and lab safety rules for the Rainbow Lab. 1 (Day 2) Prepare 3 large containers of Red, Blue, and Yellow water using food coloring. (Note: Make sure the water is saturated with food color in order to get better results). Have all lab materials available and ready for students to gather and use for the lab. Do not give the students much guidance with the lab directions, as one of the goals for this lab is for students to be able to read directions carefully. Facilitate the Rainbow lab. Discuss lab results. Columbus City Schools Curriculum Leadership and Development Science Office June 2013 (Day 2) Students follow all lab safety precautions, guidelines, and directions for the Rainbow lab using the student lab worksheet. Students complete the lab and answer post-lab questions. Students participate in a class discussion based on lab results. 2 Name_____________________________Date____________________Period____ SpongeBob’s Science Experiment A. As you read about SpongeBob’s experiment, circle all of the science/safety tools that are mentioned in the story. B. Record 10 safety rules that have been broken. 1. _______________________________________________________________________ 2. _______________________________________________________________________ 3. _______________________________________________________________________ 4. _______________________________________________________________________ 5. _______________________________________________________________________ 6. _______________________________________________________________________ 7. _______________________________________________________________________ 8. _______________________________________________________________________ 9. _______________________________________________________________________ 10. _______________________________________________________________________ Columbus City Schools Curriculum Leadership and Development Science Office June 2013 3 Name_____________________________Date____________________Period____ The Bikini Bottom group has been learning safety rules during science class. Read the paragraphs below to find the broken safety rules and underline each one. How many can you find? SpongeBob, Patrick, and Gary were thrilled when Mr. Krabbs gave their teacher a chemistry set! Mr. Krabbs warned them to be careful and reminded them to follow the safety rules they had learned in science class. The teacher passed out the materials and provided each person with an experiment book. SpongeBob and Gary flipped through the book and decided to test the properties of a mystery substance. Since the teacher did not tell them to wear the safety goggles, they left them on the table. SpongeBob lit the Bunsen burner and then reached across the flame to get a test tube from Gary. In the process, he knocked over a bottle of the mystery substance and a little bit splashed on Gary. SpongeBob poured some of the substance into a test tube and began to heat it. When it started to bubble he looked into the test tube to see what was happening and pointed it towards Gary so he could see. Gary thought it smelled weird so he took a deep whiff of it. He didn’t think it smelled poisonous and tasted a little bit of the substance. They were worried about running out of time, so they left the test tube and materials on the table and moved to a different station to try another experiment. Patrick didn’t want to waste any time reading the directions, so he put on some safety goggles and picked a couple different substances. He tested them with vinegar (a weak acid) to see what would happen even though he didn’t have permission to experiment on his own. He noticed that one of the substances did not do anything, but the other one fizzed. He also mixed two substances together to see what would happen, but didn’t notice anything. He saw SpongeBob and Gary heating something in a test tube and decided to do that test. He ran over to that station and knocked over a couple bottles that SpongeBob had left open. After cleaning up the spills, he read the directions and found the materials he needed. The only test tube he could find had a small crack in it, but he decided to use it anyway. He lit the Bunsen burner and used tongs to hold the test tube over the flame. He forgot to move his notebook away from the flame and almost caught it on fire. Before they could do another experiment, the bell rang and they rushed to put everything away. Since they didn’t have much time, Patrick didn’t clean out his test tube before putting it in the cabinet. SpongeBob noticed that he had a small cut on his finger, but decided he didn’t have time to tell the teacher about it. Since they were late, they skipped washing their hands and hurried to the next class. Adapted from the worksheet created by T. Trimpe 2003 http://sciencespot.net/ Columbus City Schools Curriculum Leadership and Development Science Office June 2013 4 Rainbow Lab - Teacher Page Goals: Be able to identify the proper equipment needed to complete the lab activity. Be able to measure chemicals using a graduated cylinder. Be able to use the metric system correctly. Be able to measure with precision and follow directions. Be able to follow correct lab safety procedures. Materials for each lab group: -test tube rack containing 6 test tubes (for holding liquids) -3 small beakers (for RED, YELLOW, and BLUE liquids) -1 small graduated cylinder (for measuring) -1 large graduated cylinder (for measuring) -1 bottle of water and large beaker (for rinsing) -1 eye dropper/pipette (for transferring liquids) -apron -safety glasses -lab tray http://www.sciencefix.com/home/2009/7/28/lesson-rainbow-volume.html Results: Test Tube Color of Liquid Amount of Liquid (mL) A Red 10mL B Orange 11mL C Yellow 10mL D Green 11mL E Blue 10mL F Purple 11mL Total Liquid in Test Tubes A-F 63mL Columbus City Schools Curriculum Leadership and Development Science Office June 2013 5 Post-Lab: (Write in full sentences) Teacher Answer Key 1. Look at your hands. Do you have any stains on your hands? Yes If so, those stains represent chemicals that would be on your skin right now! No 2. Describe your results. Were there any patterns? Be specific. The colors formed the colors of the rainbow in order (Red, Orange, Yellow, Green, ___________________________________________________________________________ Blue, Purple). Also, test tubes A, C, and E all ended with 10mL while test tubes B,D, ___________________________________________________________________________ ___________________________________________________________________________ and F all ended with 11mL. ___________________________________________________________________________ 63mL 2. Look at your directions for Part I. How many total mL of liquid did your start with? _______ Look at your results on your data table. How many total mL of liquid did you end with? What is one reason why some groups may have more or less liquid than when they started? Answers will vary. Example responses may include: Some liquid may have been ___________________________________________________________________________ ___________________________________________________________________________ left in the graduated cylinder during transfer of liquid; Error in measuring; Some ___________________________________________________________________________ liquid may have spilled; . ___________________________________________________________________________ 3. Is it important to read and follow directions exactly? Yes No What is likely to happen if you don’t thoroughly read and follow the directions? Answers will vary. ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ Clean-up checklist: 1. Carefully rinse your materials thoroughly 2. Place materials back in the proper places. 3. Wipe down your lab area with paper towels if needed. 4. Return to your seat and follow teacher directions. Columbus City Schools Curriculum Leadership and Development Science Office June 2013 6 Name_________________________________________ Date_____________________________ Period___________ PRE-LAB: RAINBOW LAB Goals: Be able to identify the proper equipment needed to complete the lab activity. Be able to measure the volume of liquids using appropriate science tools. Be able to use the metric system correctly. Be able to measure with accuracy. Be able to follow correct lab procedures and directions. Read the directions for the lab activity below, and identify the materials, science tools, and lab safety equipment you will need to complete the lab. Part I: 1. Check to make sure you have all of the materials. 2. Your test tubes should be labeled A-F. 3. Have your large empty beaker and bottle of water ready to rinse the graduated cylinders and eye dropper. 4. Go to the liquid station and collect 100mL of red liquid in one of the small beakers. Into another small beaker, collect 100mL of yellow liquid. Into the third small beaker, collect 100mL of blue liquid. Carefully take these back to your lab station. 5. Into test tube A, measure 25 mL of RED liquid. 6. Into test tube C, measure 17 mL of YELLOW liquid. 7. Into test tube E, measure 21 mL of BLUE liquid. Part II: (Work inside the green lab tray) 1. From test tube C, measure 4 mL and pour into test tube D. 2. From test tube E, measure 7 mL and pour into test tube D. Swirl carefully. 3. From test tube E, measure 4 mL and pour into test tube F. 4. From test tube A, measure 7 mL and pour into test tube F. Swirl carefully. 5. From test tube A, measure 8 mL and pour into test tube B. 6. From test tube C, measure 3 mL and pour into test tube B. Swirl carefully. 7. SAVE your results. Measure the contents of each test tube and record how many mL were found in each test tube on the data table. Calculate the total volume of all liquids combined and record your result. Columbus City Schools Curriculum Leadership and Development Science Office June 2013 7 Materials Needed: ____________________________ _____________________________ _______________________________ ____________________________ _____________________________ _______________________________ ____________________________ _____________________________ _______________________________ ____________________________ _____________________________ _______________________________ Lab Expectations Before Lab: I will _____________________________________________________ _____________________________________________________ _____________________________________________________ Which lab safety precautions/rules should we follow for this lab? Wear safety glasses Wear Apron/Lab Coat Wear Gloves Tie back long hair During Lab: I will _____________________________________________________ _____________________________________________________ _____________________________________________________ After Lab: I will _____________________________________________________ _____________________________________________________ Wear closed-toe shoes Write down 2 more lab safety rules that will apply for this lab: X____________________________________________ _____________________________________________ ______________________________ _____________________________________________________ X____________________________________________ _____________________________________________ ______________________________ Columbus City Schools Curriculum Leadership and Development Science Office June 2013 8 Name________________________________Date________________________Period_________ RAINBOW LAB PROCEDURE Part I: 1. Check to make sure you have all of the materials listed on your pre-lab. 2. Your test tubes should be labeled A-F. 3. Have your large empty beaker and bottle of water ready to rinse the graduated cylinders and pipette. 4. Go to the liquid station and collect 100mL of red liquid in one of the small beakers. Into another small beaker, collect 100mL of yellow liquid. Into the third small beaker, collect 100mL of blue liquid. Carefully take these back to your lab station. 5. Into test tube A, measure 25 mL of RED liquid. 6. Into test tube C, measure 17 mL of YELLOW liquid. 7. Into test tube E, measure 21 mL of BLUE liquid. Part II: (Work inside the green lab tray) 8. From test tube C, measure 4 mL and pour into test tube D. 9. From test tube E, measure 7 mL and pour into test tube D. Swirl carefully. 10. From test tube E, measure 4 mL and pour into test tube F. 11. From test tube A, measure 7 mL and pour into test tube F. Swirl carefully. 12. From test tube A, measure 8 mL and pour into test tube B. 13. From test tube C, measure 3 mL and pour into test tube B. Swirl carefully. 14. SAVE your results. Measure the contents of each test tube and record how many mL were found in each test tube on the data table. Calculate the total volume of all liquids combined. Data Table: Test Tube Results Test Tube Color of Liquid Amount of Liquid (mL) A B C D E F Total Liquid in Test Tubes A-F Columbus City Schools Curriculum Leadership and Development Science Office June 2013 mL 9 Post-Lab: (Write in full sentences) 1. Look at your hands. Do you have any stains on your hands? Yes If so, those stains represent chemicals that would be on your skin right now! No 2. Describe your results. Were there any patterns? Be specific. ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ 2. Look at your directions for Part I. How many total mL of liquid did your start with? _______ Look at your results on your data table. How many total mL of liquid did you end with? _______ What is one reason why some groups may have more or less liquid than when they started? ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ 3. Is it important to read and follow directions exactly? Yes No What is likely to happen if you don’t thoroughly read and follow the directions? ________________________________________________________________________________ ________________________________________________________________________________ _______________________________________________________________________________ Clean-up checklist: 1. Carefully rinse your materials thoroughly 2. Place materials back in the proper places. 3. Wipe down your lab area with paper towels if needed. 4. Return to your seat and follow teacher directions. Columbus City Schools Curriculum Leadership and Development Science Office June 2013 10