6th Grade
SAFETY
ADVANCED
PREPARATION
Thinking Like a 21st Century Scientist / Engineer:
Laboratory Procedures and Equipment
Students should follow all lab safety procedures and rules for using glassware
and liquids. If a test tube is broken, do not let students clean up the broken
glass. The teacher should dispose of broken glass appropriately by placing in a
secure receptacle for proper disposal.
Note: Even though students are using water colored with food coloring, it is
important for the students to complete this lab as if they were using chemicals
in order to reinforce proper lab safety practices. (e.g. wearing safety glasses,
aprons)
Prepare 3 containers of colored water using food coloring (red,yellow,blue)
Gather supplies for Rainbow Lab. Each group will need:
-test tube rack containing 6 test tubes (for holding liquids)…label A-F
-3 small beakers (for RED, YELLOW, and BLUE liquids)
-1 small graduated cylinder (for measuring)
-1 large graduated cylinder (for measuring)
-1 bottle of water and large beaker (for rinsing)
-1 eye dropper/pipette (for transferring liquids)
-apron
-safety glasses
- lab tray
Objective: Students will learn about proper laboratory procedures and
equipment, as well as practice following lab safety guidelines
through a SpongeBob reinforcement worksheet and by completing
a laboratory activity involving measuring and mixing colored
liquids.
ACTIVITIES
(2 days)
What is the teacher doing?
What are the students doing?
Lab Safety Reinforcement and
Rainbow Lab Pre-lab (Day 1)
Lab Safety Reinforcement and Rainbow
Lab Pre-lab (Day 1)
Distribute SpongeBob
Science WS. This lesson is
from
http://www.sciencespot.net/
Media/scimthdsafety.pdf
Distribute the Rainbow Lab
Pre-Lab. Discuss lab goals
and assist students as they
compete the pre-lab.
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Curriculum Leadership and Development
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1. Students read the SpongeBob
Experiment Scenario. Circle all of
the lab/safety tools that
SpongeBob, Patrick, and Gary
use. List 10 safety rules that they
break.
2. Students complete the pre-lab
by reading about the
experiment and determining the
equipment needed to complete
the experiment. They will also
identify lab expectations and
lab safety rules for the Rainbow
Lab.
1
(Day 2)
Prepare 3 large containers of
Red, Blue, and Yellow water
using food coloring. (Note:
Make sure the water is
saturated with food color in
order to get better results).
Have all lab materials available
and ready for students to
gather and use for the lab.
Do not give the students much
guidance with the lab
directions, as one of the goals
for this lab is for students to be
able to read directions carefully.
Facilitate the Rainbow lab.
Discuss lab results.
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Curriculum Leadership and Development
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(Day 2)
Students follow all lab safety
precautions, guidelines, and
directions for the Rainbow lab using
the student lab worksheet.
Students complete the lab and
answer post-lab questions.
Students participate in a class
discussion based on lab results.
2
Name_____________________________Date____________________Period____
SpongeBob’s Science Experiment
A. As you read about SpongeBob’s experiment, circle all of the science/safety tools that
are mentioned in the story.
B. Record 10 safety rules that have been broken.
1. _______________________________________________________________________
2. _______________________________________________________________________
3. _______________________________________________________________________
4. _______________________________________________________________________
5. _______________________________________________________________________
6. _______________________________________________________________________
7. _______________________________________________________________________
8. _______________________________________________________________________
9. _______________________________________________________________________
10. _______________________________________________________________________
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Name_____________________________Date____________________Period____
The Bikini Bottom group has been learning safety rules during science class.
Read the paragraphs below to find the broken safety rules and underline each
one. How many can you find?
SpongeBob, Patrick, and Gary were thrilled when Mr. Krabbs gave their
teacher a chemistry set! Mr. Krabbs warned them to be careful and reminded them
to follow the safety rules they had learned in science class. The teacher passed
out the materials and provided each person with an experiment book. SpongeBob
and Gary flipped through the book and decided to test the properties of a mystery
substance. Since the teacher did not tell them to wear the safety goggles, they left
them on the table.
SpongeBob lit the Bunsen burner and then reached across the flame to get a test
tube from Gary. In the process, he knocked over a bottle of the mystery substance
and a little bit splashed on Gary. SpongeBob poured some of the substance into a
test tube and began to heat it. When it started to bubble he looked into the test tube
to see what was happening and pointed it towards Gary so he could see. Gary
thought it smelled weird so he took a deep whiff of it. He didn’t think it smelled
poisonous and tasted a little bit of the substance. They were worried about running
out of time, so they left the test tube and materials on the table and moved to a
different station to try another experiment.
Patrick didn’t want to waste any time reading the directions, so he put on
some safety goggles and picked a couple different substances. He tested them
with vinegar (a weak acid) to see what would happen even though he didn’t have
permission to experiment on his own. He noticed that one of the substances did
not do anything, but the other one fizzed. He also mixed two substances together
to see what would happen, but didn’t notice anything. He saw SpongeBob and
Gary heating something in a test tube and decided to do that test. He ran over to
that station and knocked over a couple bottles that SpongeBob had left open.
After cleaning up the spills, he read the directions and found the materials he
needed. The only test tube he could find had a small crack in it, but he decided to
use it anyway. He lit the Bunsen burner and used tongs to hold the test tube over
the flame. He forgot to move his notebook away from the flame and almost
caught it on fire.
Before they could do another experiment, the bell rang and they rushed to
put everything away. Since they didn’t have much time, Patrick didn’t clean out
his test tube before putting it in the cabinet. SpongeBob noticed that he had a
small cut on his finger, but decided he didn’t have time to tell the teacher about it.
Since they were late, they skipped washing their hands and hurried to the next
class.
Adapted from the worksheet created by T. Trimpe 2003 http://sciencespot.net/
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Curriculum Leadership and Development
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Rainbow Lab - Teacher Page
Goals: Be able to identify the proper equipment needed to complete the lab activity.
Be able to measure chemicals using a graduated cylinder.
Be able to use the metric system correctly.
Be able to measure with precision and follow directions.
Be able to follow correct lab safety procedures.
Materials for each lab group:
-test tube rack containing 6 test tubes (for holding liquids)
-3 small beakers (for RED, YELLOW, and BLUE liquids)
-1 small graduated cylinder (for measuring)
-1 large graduated cylinder (for measuring)
-1 bottle of water and large beaker (for rinsing)
-1 eye dropper/pipette (for transferring liquids)
-apron
-safety glasses
-lab tray
http://www.sciencefix.com/home/2009/7/28/lesson-rainbow-volume.html
Results:
Test Tube
Color of Liquid
Amount of Liquid (mL)
A
Red
10mL
B
Orange
11mL
C
Yellow
10mL
D
Green
11mL
E
Blue
10mL
F
Purple
11mL
Total Liquid in Test Tubes A-F
63mL
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Post-Lab: (Write in full sentences) Teacher Answer Key
1. Look at your hands. Do you have any stains on your hands?
Yes
If so, those stains represent chemicals that would be on your skin right now!
No
2. Describe your results. Were there any patterns? Be specific.
The colors formed the colors of the rainbow in order (Red, Orange, Yellow, Green,
___________________________________________________________________________
Blue, Purple). Also, test tubes A, C, and E all ended with 10mL while test tubes B,D,
___________________________________________________________________________
___________________________________________________________________________
and F all ended with 11mL.
___________________________________________________________________________
63mL
2. Look at your directions for Part I. How many total mL of liquid did your start with? _______
Look at your results on your data table. How many total mL of liquid did you end with?
What is one reason why some groups may have more or less liquid than when they started?
Answers will vary. Example responses may include: Some liquid may have been
___________________________________________________________________________
___________________________________________________________________________
left in the graduated cylinder during transfer of liquid; Error in measuring; Some
___________________________________________________________________________
liquid may have spilled; .
___________________________________________________________________________
3. Is it important to read and follow directions exactly?
Yes
No
What is likely to happen if you don’t thoroughly read and follow the directions?
Answers will vary.
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
Clean-up checklist:
1. Carefully rinse your materials thoroughly
2. Place materials back in the proper places.
3. Wipe down your lab area with paper towels if needed.
4. Return to your seat and follow teacher directions.
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Name_________________________________________ Date_____________________________ Period___________
PRE-LAB: RAINBOW LAB
Goals: Be able to identify the proper equipment needed to complete the lab activity.
Be able to measure the volume of liquids using appropriate science tools.
Be able to use the metric system correctly.
Be able to measure with accuracy.
Be able to follow correct lab procedures and directions.
Read the directions for the lab activity below, and identify the materials, science tools, and lab safety equipment you will
need to complete the lab.
Part I:
1. Check to make sure you have all of the materials.
2. Your test tubes should be labeled A-F.
3. Have your large empty beaker and bottle of water ready to rinse the graduated cylinders and eye dropper.
4. Go to the liquid station and collect 100mL of red liquid in one of the small beakers. Into another small beaker, collect
100mL of yellow liquid. Into the third small beaker, collect 100mL of blue liquid. Carefully take these back to your lab
station.
5. Into test tube A, measure 25 mL of RED liquid.
6. Into test tube C, measure 17 mL of YELLOW liquid.
7. Into test tube E, measure 21 mL of BLUE liquid.
Part II: (Work inside the green lab tray)
1. From test tube C, measure 4 mL and pour into test tube D.
2. From test tube E, measure 7 mL and pour into test tube D. Swirl carefully.
3. From test tube E, measure 4 mL and pour into test tube F.
4. From test tube A, measure 7 mL and pour into test tube F. Swirl carefully.
5. From test tube A, measure 8 mL and pour into test tube B.
6. From test tube C, measure 3 mL and pour into test tube B. Swirl carefully.
7. SAVE your results. Measure the contents of each test tube and record how many mL were found in each test tube on
the data table. Calculate the total volume of all liquids combined and record your result.
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Materials Needed:
____________________________
_____________________________
_______________________________
____________________________
_____________________________
_______________________________
____________________________
_____________________________
_______________________________
____________________________
_____________________________
_______________________________
Lab Expectations
Before Lab:
I will _____________________________________________________
_____________________________________________________
_____________________________________________________
Which lab safety precautions/rules
should we follow for this lab?
Wear safety glasses
Wear Apron/Lab Coat
Wear Gloves
Tie back long hair
During Lab:
I will _____________________________________________________
_____________________________________________________
_____________________________________________________
After Lab:
I will _____________________________________________________
_____________________________________________________
Wear closed-toe shoes
Write down 2 more lab safety rules that will
apply for this lab:
X____________________________________________
_____________________________________________
______________________________
_____________________________________________________
X____________________________________________
_____________________________________________
______________________________
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Name________________________________Date________________________Period_________
RAINBOW LAB
PROCEDURE
Part I:
1. Check to make sure you have all of the materials listed on your pre-lab.
2. Your test tubes should be labeled A-F.
3. Have your large empty beaker and bottle of water ready to rinse the graduated cylinders and
pipette.
4. Go to the liquid station and collect 100mL of red liquid in one of the small beakers. Into another
small beaker, collect 100mL of yellow liquid. Into the third small beaker, collect 100mL of blue
liquid. Carefully take these back to your lab station.
5. Into test tube A, measure 25 mL of RED liquid.
6. Into test tube C, measure 17 mL of YELLOW liquid.
7. Into test tube E, measure 21 mL of BLUE liquid.
Part II: (Work inside the green lab tray)
8. From test tube C, measure 4 mL and pour into test tube D.
9. From test tube E, measure 7 mL and pour into test tube D. Swirl carefully.
10. From test tube E, measure 4 mL and pour into test tube F.
11. From test tube A, measure 7 mL and pour into test tube F. Swirl carefully.
12. From test tube A, measure 8 mL and pour into test tube B.
13. From test tube C, measure 3 mL and pour into test tube B. Swirl carefully.
14. SAVE your results. Measure the contents of each test tube and record how many mL were
found in each test tube on the data table. Calculate the total volume of all liquids combined.
Data Table: Test Tube Results
Test Tube
Color of Liquid
Amount of Liquid (mL)
A
B
C
D
E
F
Total Liquid in Test Tubes A-F
Columbus City Schools
Curriculum Leadership and Development
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mL
9
Post-Lab: (Write in full sentences)
1. Look at your hands. Do you have any stains on your hands?
Yes
If so, those stains represent chemicals that would be on your skin right now!
No
2. Describe your results. Were there any patterns? Be specific.
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
2. Look at your directions for Part I. How many total mL of liquid did your start with? _______
Look at your results on your data table. How many total mL of liquid did you end with? _______
What is one reason why some groups may have more or less liquid than when they started?
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
3. Is it important to read and follow directions exactly?
Yes
No
What is likely to happen if you don’t thoroughly read and follow the directions?
________________________________________________________________________________
________________________________________________________________________________
_______________________________________________________________________________
Clean-up checklist:
1. Carefully rinse your materials thoroughly
2. Place materials back in the proper places.
3. Wipe down your lab area with paper towels if needed.
4. Return to your seat and follow teacher directions.
Columbus City Schools
Curriculum Leadership and Development
Science Office June 2013
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